Product datasheet
Acetate Assay Kit (Colorimetric) ab204719
2 Images
Overview
Product name
Acetate Assay Kit (Colorimetric)
Detection method
Colorimetric
Sample type
Serum, Plasma, Tissue, Adherent cells, Suspension cells
Assay type
Quantitative
Sensitivity
< 20 µM
Species reactivity
Reacts with: Human
Product overview
Acetate Assay Kit (Colorimetric) (ab204719) acetate is an assay where free acetate is
converted to an intermediate in the presence of Acetate Enzyme Mix and Acetate Substrate Mix.
The intermediate reduces the probe to a colored product with strong absorbance at OD=450
nm. This kit is rapid, simple and sensitive. It can detect less than 20 µM free acetate in various
samples.
Notes
Acetate is an important component for biosynthesis. In living organisms,
acetylation/deacetylation of proteins is critical for posttranslational regulation of their functions.
When bound to CoA, Acetyl-CoA is central to metabolism of carbohydrates and fats. It is
also involved in the synthesis of biogenic compounds such as neurotransmitter acetylcholine.
Tested applications
Functional Studies
Properties
Storage instructions
Store at -20°C. Please refer to protocols.
Components
100 tests
Acetate Assay Buffer
1 x 27ml
Acetate Enzyme Mix (Lyophilized)
1 vial
Acetate Standard (Lyophilized)
1 vial
Acetate Substrate Mix (Lyophilized)
1 vial
ATP (Lyophilized)
1 vial
Probe (Lyophilized)
1 vial
Applications
1
Our Abpromise guarantee covers the use of ab204719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Functional Studies
Notes
Use at an assay dependent concentration.
Acetate Assay Kit (Colorimetric) images
Acetate Standard Curve.
Acetate Assay Kit (Colorimetric) (ab204719)
Measurement of Acetate in human serum (5
μl), fish liver (~100 μg) and egg (~200 μg).
Acetate Assay Kit (Colorimetric) (ab204719)
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Terms and conditions
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Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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