Curriculum Vitae
Terunaga Nakagawa, MD PhD
Associate Professor,
Department of Molecular Physiology and Biophysics,
Center for Structural Biology,
Vanderbilt University, School of Medicine.
Education and Training:
INSTITUTION AND LOCATION
University of Tokyo, College of Arts
and Sciences
University of Tokyo, Graduate
School of Medicine
University of Tokyo, Graduate
School of Medicine
Massachusetts General Hospital,
Harvard Medical School
Massachusetts Institute of
Technology
DEGREE
YEAR(s)
FIELD OF STUDY
1990-1992
Arts and Sciences
MD
1992-1996
Medicine
PhD
1996-2000
Cell Biology and
Anatomy
2000-2001
Neuroscience
2001-2005
Neuroscience and
Electron Microscopy
Postdoctoral
training
Postdoctoral
training
Positions:
1997-2000
Predoctoral fellow, Graduate School of Medicine, University of Tokyo,
Tokyo, Japan, with Professor Nobutaka Hirokawa
2000-2001
Postdoctoral fellow, Massachusetts General Hospital, Harvard Medical
School, with Professor Morgan Sheng
2001-2005
Postdoctoral Fellow, Massachusetts Institute of Technology, with
Professor Morgan Sheng.
2005-2012
Assistant Professor, Department of Chemistry and Biochemistry,
University of California, San Diego.
2012-present Associate Professor, Department of Molecular Physiology and
Biophysics, Center for Structural Biology
Vanderbilt University, School of Medicine.
Honors:
1) Hajime Hagiwara travelling fellowship (DNAX research institute, Palo Alto, CA, USA)
(1993)
2) Japan Society for the Promotion of Science Research Fellowship for Young
Scientists (1997-2000)
3) Human Frontier Science Program Long-term Fellowship (2000-2003)
4) Howard Hughes Medical Institute postdoctoral research associate (2003-2005)
5) Hellman Faculty Fellow Award (2007)
6) John Merck Fund Faculty Award (2007-2010)
7) NARSAD Young Investigator Award (2008-2009)
8) NIH EUREKA (Exceptional and Unconventional Research Enabling Knowledge
Acceleration) award (2010-2014)
9) Kazato Prize (2012) (http://www.kazato.org/english/kazato_prize_info.html)
Peer reviewed primary publications:
1) Nakagawa, T., Chen, J., Zang, Z., Kanai, Y., and Hirokawa, N. (1995) Two distinct
functions of the carboxyl-terminal tail domain of NF-M upon neurofilament assembly:
cross-bridge formation and longitudinal elongation of filaments. J. Cell. Biol. 129:
411-429.
2) Nakagawa, T., Tanaka, Y., Matsuoka, E., Kondo, S., Okada, Y., Noda, Y., Kanai, Y.,
and Hirokawa, N. (1997) Identification and classification of sixteen new kinesin
superfamily (KIF) proteins in mouse genome. Proc. Natl. Acad. Sci. USA 94: 96549659.
3) Setou, M., Nakagawa, T., Seog. D.–H., and Hirokawa, N. (2000) Kinesin superfamily
motor protein KIF17 and mLin-10 in NMDA receptor-containing vesicle transport.
Science 288: 1796-1802.
4) Nakagawa, T., Setou, M., Seog, D.–H., Ogasawara, K., Dohmae, N., Takio, K., and
Hirokawa, N. (2000) A novel motor, KIF13A, transports mannose-6-phosphate
receptor to plasma membrane through direct interaction with AP-1 complex. Cell
103: 569-581.
5) Zhao, C., Takita, J., Tanaka, Y., Setou, M., Nakagawa, T., Takeda, S., Yang, H. W.,
Terada, S., Nakata, T., Takei, Y., Saito, M., Tsuji, S., Hayashi, Y., and Hirokawa, N.
(2001) Charcot-Marie-Tooth disease type 2A caused by mutation in a microtubule
motor KIF1Bβ. Cell 105: 587-597.
6) Nakajima, K., Takei, Y., Tanaka, Y., Nakagawa, T., Nakata, T., Noda, Y., Setou, M.,
and Hirokawa, N. (2002) Molecular motor KIF1C is not essential for mouse survival
and motor-dependent retrograde Golgi apparatus-to-endoplasmic reticulum
transport. Mol. Cell Biol. 22: 866-873.
7) Passafaro, M., Nakagawa, T., Sala, C., and Sheng, M. (2003) Induction of dendritic
spines by an extracellular domain of AMPA receptor subunit GluR2. Nature 424:
677-681.
8) Nakagawa, T., Engler, J. A., and Sheng, M. (2004) The dynamic turnover and
functional roles of α-actinin in dendritic spines. Neuropharmacology 47: 734-745.
9) Schulz, T. W., Nakagawa, T., Licznerski, P., Pawlak, V., Kolleker, A., Rozov, A.,
Kim, J., Dittgen, T., Köhr, G., Sheng, M., Seeburg, P. H., and Osten, P. (2004)
Actin/α-actinin-dependent transport of AMPA receptors in dendritic spines: role of the
PDZ-LIM protein RIL. J. Neurosci. 24: 8584-8594.
10) Nakagawa, T., Futai, K., Lashuel, H. A., Lo, I., Okamoto, K., Walz, T., Hayashi, Y.,
and Sheng, M. (2004) Quaternary structure, protein dynamics and synaptic function
of SAP97 controlled by L27 domain interactions. Neuron 44: 453-467.
11) Nakagawa, T., Cheng, Y., Ramm, E., Sheng, M., and Walz, T. (2005) Structure and
different conformational states of native AMPA receptor complexes. Nature, 433:
545-549.
12) Takao, K., Okamoto, K., Nakagawa, T., Neve, R. L., Nagai, T., Miyawaki, A.,
Hashikawa, T., Kobayashi, S., and Hayashi, Y. (2005) Visualization of synaptic Ca2+
/calmodulin-dependent protein kinase II activity in living neurons. J. Neurosci. 25:
3107-3112.
13) Nakagawa, T., Feliu-Mojer, M. I., Wulf, P., Lois, C., Sheng, M., and Hoogenraad, C.
C. (2006) Generation of lentiviral transgenic rats expressing glutamate receptor
binding protein 1 (GRIP1) in brain, spinal cord, and testis. J. Neurosci. Methods
152: 1-9.
14) Chen, X., Vinade, L., Leapman, R. D., Petersen, J.D., Nakagawa, T., Phillips, T. M.,
Sheng, M., and Rees, T. S. (2005) Mass of the post synaptic density and
enumeration of three key molecules. Proc. Natl. Acad. Sci. USA 102: 11551-11556.
15) Nakagawa, T., Cheng, Y., Sheng, M., and Walz, T. (2006) Three-dimensional
structure of an AMPA receptor without associated stargazin/TARP proteins. Biol.
Chem. 387: 179-187.
16) Cheng, D., Hoogenraad, C. C., Rush, J., Ramm, E., Schlager, M. A., Duong, D. M.,
Xu, P., Wijayawardana, S. R., Hanfelt, J., Nakagawa, T., Sheng, M., and Peng, J.
(2006) Relative and absolute quantification of postsynaptic density proteome isolated
from rat forebrain and cerebellum.Mol. Cell. Proteomics 5: 1158-1170.
17) Fischer, A., Garcia-Rodriguez, C., Geren, I., Lou, J., Marks, J. D., Nakagawa, T.*,
and Montal, M.* (* both senior authors contributed equally to this work)
(2008)
Molecular architecture of Botulinum neurotoxin E revealed by single particle electron
microscopy.
J. Biol. Chem. 283: 3997-4003.
18) Shanks, N. F., Maruo, T., Farina, A. N., Ellisman, M. H., and Nakagawa, T. (2010)
Contribution of the global subunit structure and stargazin on the maturation of AMPA
receptors. J. Neurosci. 30: 2728-2740.
19) Comoletti, D., Miller, M., Jeffries, C. M., Wilson, J., Demeler, B., Taylor, P., Trewhella,
J., and Nakagawa, T. (2010) The macromolecular architecture of the extracellular
domain of a-neurexin-1: domain organization, flexibility and insights into transsynaptic association. Structure 18: 1044-1053.
20) Farina, A. N., Blain, K. Y., Maruo, T., Kwiatkowski, W., Choe, S., and Nakagawa, T.
(2011) Separation of domain contacts is required for heteromeric assembly of
functional NMDA receptors. J. Neurosci. 31: 3565-3579.
21) Shanks, N. F., Savas, J. N., Maruo, T., Cais, O., Hirao, A., Oe, S., Ghosh, A., Noda,
Y., Greger, I. H., Yates III, J. R., Nakagawa, T. (2012) Differences of AMPA and
kainate receptor interactomes facilitate identification of AMPA receptor auxiliary
subunit, GSG1L. Cell Reports 1: 590-598.
Other publications:
1) Nakagawa, T., and Sheng, M. (2000) A stargazer fortells the way to the synapse.
Science 290: 2270-2271. (invited review)
2) Sheng, M., and Nakagawa, T. (2002) Neurobiology: glutamate receptors on the
move. Nature 417: 601-602. (invited review)
3) Nakagawa, T. (2010) The biochemistry, ultrastructure, and subunit assembly
mechanism of AMPA receptors. Mol. Neurobiol. 42: 161-184.
4) Nakagawa, T., Hoogenraad, C. C. (2011) Lentiviral transgenesis. Methods Mol.
Biol. 693: 117-142. In Transgenic Mouse Methods and Protocols. 2nd Edition.
Chapter 8. (Editors Hofker, M., van Deursen, J.).
Presentations:
2012:
May 14 Kazato Prize Lecture (The Japanese Society for Microscopy), Tsukuba, Japan
Lecture: The molecular mechanism of subunit assembly of glutamate receptors.
May 17 JEOL, Akishima, Tokyo, Japan
Lecture: Single particle electron microscopy in neuroscience
Feb 16 Vanderbilt University Medical School, Department of Molecular Physiology and
Biophysics
Seminar: Ionotropic glutamate receptors: assembly mechanism and interactions with
auxiliary subunits
2011:
Mar 28-30 The 88th Annual Meeting of the Physiological Society of Japan
The 116th Annual Meeting of the Japanese Association of Anatomists
Symposium Speaker: The molecular mechanism of subunit assembly of glutamate
receptors. (Meeting cancelled because of earthquake)
2010:
Dec 17 MRC Laboratory for Molecular Biology, Cambridge, UK
Seminar: Molecular requirement for the subunit assembly of functional NMDA receptors.
Apr 15 San Diego State University
Seminar: The structures of dimer intermediates of AMPA receptors during subunit
assembly.
2009:
Jun 8 CNR Institute of Neuroscience, Cellular and Molecular Pharmacology, Milan, Italy
Seminar: The structures of dimer intermediates of AMPA receptors during subunit
assembly.
Jun 4 ENP meeting (The Dutch Neuroscience Meeting), The Netherlands
Symposium: Hot topics in AMPA-receptor trafficking
Seminar: Molecular anatomy of AMPA receptors during subunit assembly.
Jun 3 Neuroscience Department, Erasmus Medical Center, Rotterdam, The
Netherlands
Seminar: Molecular structures of mature and immature AMPA type glutamate receptors.
May 30 UCSD Neuroscience Graduate Programs Retreat
Seminar: Molecular anatomy of AMPA receptor complexes
2008:
Dec 26 Global COE Special Seminar, Gunma University School of Medicine, Japan
Seminar: Molecular anatomy of AMPA receptor complexes during maturation
2007:
Dec 27 Kyoto University, Department of Biophysics, Japan
Seminar: Ultrastructure of protein complexes in synapses.
Dec 26 Keio University, Medical School, Japan
Seminar: Ultrastructure of protein complexes in synapses.
Dec 26 University of Tokyo, Graduate School of Medicine, Japan
Seminar: Ultrastructure of protein complexes in synapses
Sept 13 Japan/US Nanotechnology Forum, Hillsboro, Oregon.
Seminar: Detecting nanostructures in synapses.
2005:
Nov 19 UCSD Molecular Biophysics Training Grant Retreat
Seminar: Three-dimensional structure of an AMPA receptor without associating
stargazin/TARP proteins.
Nov 14 Salk Institute
Seminar: Structure and different conformational states of native AMPA receptor
complexes. (host: Senyon Choe)
Nov 7-8 Osaka University, Osaka, Japan
COE International Symposium: Structure of AMPA receptor complexes.
Seminar: Assembly and reconstitution of membrane proteins and cellular molecular
machineries.
Oct 20 Forum at Scripps Institute: Automated Molecular Imaging (AMI)
Seminar: Structure of AMPA subtype of glutamate receptors.
Jun 6-8 Picower Center for Learning and Memory Retreat, MIT
Seminar: Structure and different conformational states of native AMPA receptor
complexes.
May 23 Tokyo Medical and Dental University, Tokyo, Japan
Seminar: Structure and different conformational states of native AMPA receptor
complexes.
Apr 27 Harvard Medical School, Single Molecule Meeting
Seminar: Structure and different conformational states of native AMPA receptor
complexes.
Mar 14-16 Howard Hughes Medical Institute, Janelia Farm Symposium
Seminar: Imaging of sub-synaptic modular structures by cryo-EM and single particle
analysis.
Feb 9 Harvard University, Department of Molecular and Cellular Biology
Seminar: Structure and different conformational states of native AMPA receptor
complexes.
Jan 11 Brandeis University, Department of Biology
Seminar: Structure and different conformational states of native AMPA receptor
complexes.
Jan 3 University of California, San Diego, Department of Chemistry and Biochemistry
Seminar: Structure and different conformational states of native AMPA receptor
complexes.
2004:
Dec 10 Washington University, St. Louis, Department of Anatomy and Neurobiology
Seminar: 3D structure and different conformational states of native AMPA receptor
complexes.