The OREGIN Diversity Demonstration Field Trials
http://www.oregin.info/
C.J. Hopkins1, S.J. Welham1, G.R.Teakle2, K‐S. Peplow2, D.A.C. Pink3, P.W.C .Carion1, G.J. King4 & J.H.A. Barker1
1Rothamsted Research (RRES), Harpenden, Herts, AL5 2JQ, UK; 2University of Warwick, Wellesbourne, CV35 9EF, UK; 3Harper Adams University College, Shropshire, TF10 8NB (formerly at
Univ. Warwick); 4Southern Cross University, PO Box 157, Lismore, NSW 2480, Australia (formerly at RRES)
*Additional contributors involved in the success of this project are acknowledged below
05/05/2010
The
project (funded by Defra) has assembled key genetic resources to enable researchers and breeders to explore the
relevant genepool for enhanced traits to incorporate into breeding programmes. This includes establishing Diversity Fixed Foundation
Sets for B. napus (BnaDFFS); accessions listed at: www.brassica.info/resource/plants/diversity_sets.php). The founder lines within the
BnaDFFS were compiled to represent a structured sampling of the genetic diversity across the global B. napus genepool, and to
encompass winter and spring OSR, swedes, and fodder, forage and salad kales. UK oilseed breeders are assisting with completing
single seed descent (SSD) fixation and bulking of fixed lines.
Winter Trial 2009/2010 (yr1); 2 reps; high/low N; 61 genotypes Replicate 1
‐N
‐N
Replicate 2
14/07/2010
+ N
+ N
In 2009,
established
small-scale demonstration trials
over two seasons (2009/10;
2010/11) at Rothamsted Research
to observe, sample and collect
baseline information describing
plant performance and properties
of the BnaDFFS.
A key feature of the OREGIN Diversity Demonstration Trials is that they are available for use by the research community..
This was highly successful for the 2009/2010 trial; >100 people visited and >60 traits were scored collaboratively.
Trait assessments from the Winter Trial 2009/2010 (yr1) included: •Simple photographic records
•Sampling, mineral analyses & archive of early/canopy leaves
•Pigeon damage
•Flowering phenology
•Pollen beetle numbers •Beneficial insect use
•Disease severity assessment of ‐Phoma leaf spot & stem canker (Leptosphaeria maculans)
‐Light leaf spot (Pyrenopeziza brassicae)
‐Sclerotinia stem rot (Sclerotinia sclerotiorum)
‐Powdery mildew (Erisiphe polygoni)
•Pod senescence & dehiscence
•Plant height, maturity and stem stiffness •Straw composition analyses
•Seed & silique size, number & seed composition
Trait data will be secured in ___________ and made available via
the ______ website. Raw data for 2009/10 have been collated
and analysed in GenStat® providing REML means, scatter plots,
distribution of line means, heritability values and outputs for other
software. We are currently processing post-harvest traits and
further examining trait variation and patterns of co-variation across
traits. GxE interactions will be investigated with 2010/11 trial data
and other relevant datasets.
Groups of variables show strong correlations
OREGIN 2009/10: Correlation matrix for line predictions for all pre‐harvest variables (transformed scale)
Winter/Spring Trial 2010/2011 (yr2); 2 reps; low N only; 205 genotypes 21/03/2011
We strongly encourage anyone who wishes to view,
score or sample the current Winter/Spring Trial to contact:
jackie.barker@bbsrc.ac.uk
Acknowledgements: For additional assessments: Jon West, Yongju Huang, James Townsend, Sam Cook, Andrew Ferguson, Matthew Skellern, Nigel Watts, Andrew Stoute, summer
students (Rothamsted Research); Will Spracklen, Rory Hayden (WHRI), Lei Shi, Jing Wang (Huazhong Agricultural University, China); Colin Morgan, Andrea Harper, summer students (John
Innes Centre); Jerry Roberts, John Hammond (Univ. Nottingham); Carol Wagstaff, Emma Bennett, Leo Shum (Univ. Reading); Simon Kightley, Jane Thomas, Amanda Cottage, Sandra
Chapman, Simon Griffiths (NIAB). For general advice &/or seed production (UK OSR Breeders) : Jo Bowman (Nickerson, Limagrain UK Ltd); Richard Jennaway (Saaten Union UK Ltd.); Mark
Nightingale (Elsoms Seeds Ltd.); Peter Werner (KWS UK Ltd.); Ian Bancroft (John Innes Centre); Pete Berry, Peter Gladders (ADAS); RRES Farm Staff & all other members of the OREGIN
Management Committee.
In collaboration with: