Peptide factors: Urocortin
brane
Cell mem
Cytokines,
Growth factors
Cardiac stress:
I/R, ROS, MI
GPCR
Cardioprotective Signaling
in the Stress-Induced
Myocardium
JAK
JAK
ROS
Tyrosine kinases
P
P
PKCε
P
iPLA2
GTP
Ras
SHP2
?
STAT
STAT
EGCG
P
LPC
Cytokines and peptide growth factors including interferons and urocortin, for example,
transmit through intracellular signaling pathways which mediate transcriptional activity
and regulate cardioprotection. Transcriptional activity is regulated through a number
of intermediates in the cardiac myocyte and includes PKC and iPLA2/LPC pathways.
These two pathways can converge on the mitochondrion and help regulate
mitochondrial permeability either through the MPTP or the Kir6.1 channels (potassium
inward rectifier). Through phosphorylation activity, the AKT/mTor pathway regulates
protein synthesis (and subsequently cell volume - hypertrophy) and the MAPK kinase
pathway controls transcription of cardiac survival genes.
www.abcam.com/cardiovascular
Produced in collaboration with Dr Paul Townsend (University of Southampton) and Abcam
PIAS,
SOCS1/2,
PTPs
Serine kinase
PKCε
MPTP
MEK
AKT
U0126,
PD98059
Kir6.1
P P
STAT
mTor
ERK
STAT degradation
STAT
P P
Cardiac stress such as I/R injury, ROS, or MI mediate transcriptional responses via the
STAT factors. STAT1 mediates cell death pathways, whereas STAT3 mediates
cytoprotection. Some inhibitors of the STAT pathways are highlighted: 1) U0126 and
PD98059 are pharmacological ERK inhibitors; 2) EGCG, the main constituent of green
tea can inhibit STAT1 tyrosine and serine phosphorylation; and 3) PIAS, SOCS and PTPs
are endogenous cellular inhibitors of the signaling routes critical in cardioprotection.
Abbreviations:
EGCG: Epigallocatechin-3-gallate; GPCR: G protein coupled receptor; I/R:
ischemia/reperfusion; JAK: Janus activated kinase; MI: Myocardial infarction; MPTP:
Mitochondrial permeability pore; PIAS: Protein inhibitor of activated STATs; PKC:
protein kinase C; PTPs: protein tyrosine phosphatases; ROS: reactive oxygen
species; SOCS: Silencers of cytokine signaling; STAT: Signal transducer and
activator of transcription; and TF: transcription factor
Raf
PI3K
?
4EBP1
Cytoplasm
Importins
p70S6K
Protein synthesis
modulation
ERK
TF
TF
p53
P
P P
p300
Nucleus
Bag1, IAP, HSP27, HSP70, STAT3
Cell death
via STAT1
STAT
STAT
Cardiac survival genes:
Transcription
P P
p73
Cardioprotection
via STAT3
Cardiovascular antibodies from Abcam:
Product
Clonality Applications
Host
A RAF
AKT
AKT1 (phospho S473)
AKT1 [Y89]
AKT2
P
P
P
M
P
ELISA, IHC-P, WB
ICC, IHC-P, WB
ELISA, IHC-P, WB
Flow Cyt, ICC, IHC-P, IP, WB
ELISA, IHC-P, IP, WB
Rb
Rb
Rb
Rb
Rb
AKT3
B Raf [EP152Y]
Bag1 [Y166]
eIF4EBP1 [Y329]
ERK1 + ERK2 (phospho T183 + Y185)
[MAPK-YT]
ERK1 + ERK2
ERK1 [Y72]
ERK2 [E460]
Hsp27 (phospho S86)
Hsp27 [IAP-28]
P
M
M
M
ELISA, IP, WB
Flow Cyt, IHC-P, IP, WB
ICC/IF, IHC-P, IP, WB
Flow Cyt, ICC, IHC-P, IP, WB
Rb
Rb
Rb
Rb
M
P
M
M
P
M
ELISA, ICC/IF, IHC-P, IP, WB
IHC-Fr, IHC-P, WB
Flow Cyt, ICC, IHC-P, IP, WB
Flow Cyt, ICC, IHC-P, IP, WB
ICC/IF, IHC-Fr, WB
I-ELISA, ICC/IF, IHC-Fr, RIA, WB
Ms
Rb
Rb
Rb
Rb
Ms
Hsp70 [C92F3A-5]
M
ELISA, Flow Cyt, ICC/IF, IHC-P, IP, WB
Ms
KAT3B / p300 [NM11]
MEK1 (phospho S221)
MEK1 [E342]
M
P
M
ICC/IF, IHC-P, IP, WB
ELISA, IHC-P, WB
Flow Cyt, ICC/IF, IHC-P, IP, WB
Ms
Rb
Rb
Species
Reactivity
See more products at: www.abcam.com
Datasheet
www.abcam.com/ab…
Hu, Ms, Rat
Hu, Ms, Rat, Ck*
Hu, Ms
Hu, Ms, Rat. Cow*
Hu, Ms, Rat, Chmp*, Cow*,
Dog*, RMk*
Hu, Ms, Rat
Hu, Ms
Hu, Rat
Hu, Ms, Rat
Hu, Ms, Rat, Cow, Dm, Sc, Xl*, Zfsh*
Hu, Ms, Rat, Chk*, Xl*, Zfsh*
Hu, Ms
Hu, Ms, Rat
Hu, Ms, Rat
Hu, Ms, Rat, Chk, Cow, Dm,
Gpig, Rb, Trky, Xl
Hu, Ms, Rat, Ce, Carp, Chk, Cow,
Dog, Dm, Gpig, Hm, Mk, Pig, Rb, Shp
Hu, Ms, Rat, Mink, Mk, Pig
Hu, Ms, Rat
Hu, Ms, Rat, Cow
59343
8805
28821
32505
66129
5922
33899
32109
32024
50011
76163
32537
32081
17938
49919
47455
3164
47291
32091
pan-AKT antibody (ab8805)
Clonality
P
Applications
ICC, IHC-P, WB
Species cross reactivity
Hu, Ms, Rat, Ck*
p73 antibody [EP436Y] (ab40658)
Applications
ICC/IF, IHC-P, IP, WB
Clonality Applications
MEK2 (phospho T394)
MEK2
mTor
mTor
p53 (phospho S15)
p53
P
P
M
P
P
M
P70 S6 Kinase beta (phospho T229)
P70 S6 Kinase beta
p73 (phospho Y99)
p73 [EP436Y]
PI 3 Kinase p85 alpha (phospho Y607)
PI 3 Kinase p85 alpha [ep380y]
PKC
P
P
P
M
P
M
M
PKC epsilon
Ras [F132-62]
SHP2
SOCS1 - Carboxyterminal end
SOCS2
STAT1 (phospho Y701)
STAT1
STAT3 [E121-21]
P
M
P
P
P
P
P
M
Host
ELISA, IHC-P, IP, WB
ELISA, IHC-P, IP, WB
Flow Cyt, ICC/IF, IHC-P, IP, WB
ICC, IHC-Fr, IP, WB
ELISA, ICC/IF, IHC-P, IP, WB
ELISA, Flow Cyt, ICC/IF, IHC-Fr,
IHC-P, IP, WB
ELISA, IHC-P, WB
ELISA, IHC-P
ELISA, IHC-P, WB
ICC/IF, IHC-P, IP, WB
ELISA, IHC-P, WB
Flow Cyt, ICC/IF, IHC-P, IP, WB
IHC-P, ICC, WB, ELISA, RIA,
IP, IHC-FoFr
WB, ELISA, ICC/IF
ICC/IF, IHC-Fr, IHC-P, IP, WB
ELISA, IHC-P, WB
ICC, IHC-P, WB
ELISA, IP, WB
ELISA, IHC-P, IP, WB
ELISA, IHC-P, IP, WB
IHC-Fr, IHC-P, IP, WB
Species
Reactivity
Datasheet
www.abcam.com/ab…
Rb
Rb
Rb
Rb
Rb
Hu,
Hu,
Hu,
Hu,
Hu
Ms, Rat
Ms
Ms, Rat
Rat
30622
28834
32028
2732
38497
Ms
Rb
Rb
Rb
Rb
Rb
Rb
Hu
Hu,
Hu,
Hu,
Hu,
Hu,
Hu,
Ms
Rb
Ms
Rb
Rb
Rb
Rb
Rb
Rb
Hu, Ms, Rb
Rat, Hu*, Ms*, Rb*
Hu, Ms, Rat
Hu, Ms, Rat
Hu, Ms, Rat, Rb
Hu, Ms, Rat
Hu, Ms, Rat
Hu, Ms, Rat
Hu, Rat, Cow*, Hrs*
1101
59208
52169
38457
40658
61801
40755
Ms, Rat
MS, Rat
Ms, Rat
Ms, Rat
Ms, Rat
Rat
31
74274
16907
51162
65989
3692
30643
31369
32500
Hsp27 antibody [G3.1] (ab2790)
Host
Rb
The Akt antibody (ab8805) is used at 1/80 on cultured neonatal rat cardiomyocytes that express a nucleartargeted Akt construct. The green is Akt antibody, the red is Texas-red™ phalloidin that labels actin filaments.
Clonality
M
Product
Host
Rb
Species cross reactivity
Hu, Ms, Rat
Paraffin-embedded human urinary bladder carcinoma using ab40658 at a 1/100 dilution.
Clonality
M
Applications
ELISA, ICC/IF, IHC-P, IP, WB
Host
Ms
Species cross reactivity
Hu, Ms*
Cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of
5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer
was removed and primary antibody was added at a dilution of 1/200 and incubated overnight at 4°C.
PI 3 Kinase p85 alpha antibody [ep380y] (ab40755)
Clonality
M
Applications
Flow Cyt, ICC/IF, IHC-P, IP, WB
Host
Rb
Species cross reactivity
Hu, Rat
Staining of PI 3 Kinase p85 alpha on paraffin-embedded human breast carcinoma, using ab40755 at a 1:100 dilution.
Clonality
P
Applications
ICC, IHC-Fr, IP, WB
Bag1 antibody [Y166] (ab32109)
Host
Rb
Species cross reactivity
Hu, Rat
ab2732 at a 1:100 dilution confocally staining mTOR (red) in L6 myotubes, alongside a nuclear
antigen antibody (green).
*predicted to react based on sequence homology
Clonality
M
Applications
ICC/IF, IHC-P, IP, WB
Host
Rb
Immunofluorescent staining of HeLa cells using ab32109 at 1/100 dilution.
Species cross reactivity
Hu, Rat
042-03/09-PW
mTOR antibody (ab2732)