DNA Extraction
DNA Extraction from Plant Material
DNA is extracted onto the
paper matrix.
The FTA card is a chemically
treated paper matrix for the
safe collection, transport and
storage of DNA.
Before PCR, inhibitors of the
reaction have to be removed
from the card.
DNA Extraction from Plant Material
When a plant sample is pressed onto the FTA
matrix, moisture in the sample activates
chemicals in the FTA card and these chemicals
lyse the cells, denature enzymes, inactivate
pathogens and immobilise the DNA.
Once completely dry, such plant extracts can be
stored for several years at room temperature.
This method is not suitable for fibrous leaves
such as cabbage or pine, which are difficult to
crush.
DNA Extraction from Plant Material
Place a
backing board
between the
back cover of
the card and
the absorbent
layer.
DNA Extraction from Plant Material
Place a piece of
fresh plant
material onto
one box on the
FTA card.
Ensure it does
not extend
outwith the box.
Close cover.
DNA Extraction from Plant Material
Using a pestle, squash the leaf onto the
card until moisture has soaked through
to the back of the absorbent layer.
Discard squashed plant material.
Try to keep the sample within the box.
DNA Extraction from Plant Material
Repeat extraction for a
second plant material
in a different box.
Try to ensure each
sample stays within
its own box.
DNA Extraction from Plant Material
Make sure that
moisture from
the leaf has
soaked through
to the back of
the paper.
DNA Extraction from Plant Material
Repeat extraction for
the third and fourth
plant materials in the
remaining boxes.
Try to ensure there is no
‘escape’ of extracted
materials to other boxes.
DNA Extraction from Plant Material
Label
your
samples.
DNA Extraction from Plant Material
Leave cards
open to dry.