Product datasheet
Anti-PGP9.5 antibody [BH7] ab64722
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Overview
Product name
Anti-PGP9.5 antibody [BH7]
Description
Mouse monoclonal [BH7] to PGP9.5
Tested applications
WB, ICC/IF
Species reactivity
Reacts with: Rat, Cow, Human
Immunogen
Recombinant full length human UCHL1 purified from E. coli.
Positive control
Rat spinal cord, brain, SHSY-5Y, HEK293 cell extract or whole cell homogenate of bovine brain.
General notes
This antibody is made in an Integra CL350 flask, with total protein content about 10mg/ml, of
which about 1mg/ml is antibody.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term.
Storage buffer
Preservative: 10mM Sodium Azide
Constituents: Tissue culture supernatant
Purity
Ascites
Purification notes
Ascites fluid
Clonality
Monoclonal
Clone number
BH7
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab64722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/20000. Detects a band of approximately 24 kDa (predicted molecular weight:
25 kDa).
ICC/IF
1/2000.
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Target
Anti-PGP9.5 antibody [BH7] images
Lanes 1 - 2 : A monoclonal antibody reactive
with PGP9.5
Lane 3 : Anti-PGP9.5 antibody [BH7]
(ab64722) at 1/10000 dilution
Lane 1 : Whole cell homogenate of bovine
brain
Lane 2 : Whole cell homogenate of bovine
brain
Lane 3 : Whole cell homogenate of bovine
brain
Western blot - PGP9.5 antibody [BH7] (ab64722)
Predicted band size : 25 kDa
Observed band size : 24 kDa
Immunofluorescent staining of rat spinal cord
with ab64722 (1:1000, red). These large cells
are a-motorneurons and UCHL1 protein is a
major component of the perikarya and
dendrites of these cells.
Immunocytochemistry/ Immunofluorescence PGP9.5 antibody [BH7] (ab64722)
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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