Product datasheet
Anti-Lactate Dehydrogenase antibody (HRP) ab7639
5 Abreviews 10 References 2 Images
Overview
Product name
Anti-Lactate Dehydrogenase antibody (HRP)
Description
Goat polyclonal to Lactate Dehydrogenase (HRP)
Conjugation
HRP
Conjugation notes
Horseradish Peroxidase (HRP)
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as
well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].
Tested applications
IHC-P, Dot Blot, ELISA, Immunomicroscopy, WB
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Human
Immunogen
Full length native Lactate Dehydrogenase (purified from rabbit muscle).
Positive control
WB: purified enzyme from rabbit muscle (Worthington Biochemicals) IHC-P: non-small-cell lung
cancer (see Koukourakis et al)
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Purity
IgG fraction
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step
process which includes delipidation, salt fractionation and ion exchange chromatography
followed by extensive dialysis against the buffer stated above.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab7639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-P
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Application
Abreviews
Notes
Dot Blot
ELISA
Immunomicroscopy
WB
Application notes
Dot: Use at an assay dependent dilution.
ELISA: 1/1000 - 1/5000.
IHC-P: 1/200. Quench peroxidases with methanol and H2O2 3% for 15 min. Perform heat
mediated antigen retrieval via the microwave method (3x5 min).before commencing with IHC
staining protocol. Incubate antibody overnight. A modified streptavidin technique was used (Kwik
kit) (see Koukourakis MI et al reference).
IM: Use at an assay dependent dilution.
WB: 1/1000-1/10000 (see Abreview). Please note: The non-conjugated version of this antibody
(ab2101) has been used in WB in the reference Zaman K et al (protocol available on the
datasheet for ab2101).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in disease
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A
metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm.
Anti-Lactate Dehydrogenase antibody (HRP) images
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All lanes : Anti-Lactate Dehydrogenase
antibody (HRP) (ab7639) at 1/2000 dilution
Lane 1 : U2OS whole cell lysate (Hu)
Lane 2 : 293T whole cell lysate (Hu)
Western blot - Lactate Dehydrogenase antibody
(HRP) (ab7639)
Lane 3 : Cos-7 whole cell lysate (AGMk)
Lane 4 : CHO whole cell lysate(Hm)
Lane 5 : PC12 whole cell lysate (Rat)
Lane 6 : 3T3 whole cell lysate (Ms)
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Observed band size : 35 kDa
This image is courtesy of an anonymous
Abreview
Anti-Lactate Dehydrogenase antibody (HRP)
(ab7639) at 1/1000 dilution + Lactate
Dehydrogenase protein (ab77857) at 0.01 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L - PreAdsorbed (HRP) (ab65486) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Western blot - Anti-Lactate Dehydrogenase
Exposure time : 10 seconds
antibody (HRP) (ab7639)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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