Product datasheet
Anti-Lactate Dehydrogenase antibody [AF14A11]
ab85326
1 Abreviews 1 References 2 Images
Overview
Product name
Anti-Lactate Dehydrogenase antibody [AF14A11]
Description
Mouse monoclonal [AF14A11] to Lactate Dehydrogenase
Tested applications
Flow Cyt, WB, IP, ELISA
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
His tagged recombinant Human Lactate Dehydrogenase protein purified from E.coli.
Positive control
HeLa, MCF7, HepG2, 293T, SK-N-MC, RD and IMR32 cell lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
Purity
Ammonium Sulphate Precipitation
Clonality
Monoclonal
Clone number
AF14A11
Isotype
IgG2b
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab85326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Flow Cyt
Abreviews
Notes
1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype
control with this antibody.
WB
1/2000 - 1/10000. Detects a band of approximately 30 kDa.
IP
Use at an assay dependent concentration. Use 0.5µg
1
Application
Abreviews
ELISA
Notes
1/2000 - 1/10000.
Target
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in disease
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A
metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm.
Anti-Lactate Dehydrogenase antibody [AF14A11] images
All lanes : Anti-Lactate Dehydrogenase
antibody [AF14A11] (ab85326) at 1/2000
dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Western blot - Lactate Dehydrogenase antibody
Lane 5 : SK-N-MC cell lysate
[AF14A11] (ab85326)
Lane 6 : RD cell lysate
Lane 7 : IMR32 cell lysate
Observed band size : 30 kDa
2
Overlay histogram showing HeLa cells
stained with ab85326 (red line). The cells
were fixed with 80% methanol (5 min) and
then permeabilized with 0.1% PBS-Tween for
20 min. The cells were then incubated in 1x
PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein
interactions followed by the antibody
Flow Cytometry-Anti-Lactate Dehydrogenase
(ab85326, 1/100 dilution) for 30 min at 22ºC.
antibody [AF14A11](ab85326)
The secondary antibody used was DyLight®
488 goat anti-mouse IgG (H+L) (ab96879) at
1/500 dilution for 30 min at 22ºC. Isotype
control antibody (black line) was mouse
IgG2b [PLPV219] (ab91366, 2µg/1x106 cells)
used under the same conditions. Acquisition
of >5,000 events was performed. This
antibody gave a positive signal in HeLa cells
fixed with 4% paraformaldehyde (10
min)/permeabilized with 0.1% PBS-Tween for
20 min used under the same conditions.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
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