National 5 Biology Resources Guide November 2014 NATIONAL 5 BIOLOGY RESOURCES GUIDE Biology National 5 Resources Guide This resource guide has been produced in response to requests from staff who attended the NQ Sciences events at Hampden Stadium in December 2013. Those attending felt it would be useful to have a document which helped them navigate to the most relevant resources quickly. The following pages show the mandatory course key areas table from the SQA Biology National 5 Course and Unit Support Notes. An additional fourth column has been included which contains hyperlinks to useful resources. Please note: Staff are not required to use the resources listed – they are only included as helpful suggestions. Staff should also refer to the SQA website for the most up-to-date course and unit support notes. To further assist staff links to useful SQA documentation have been included at the beginning of each unit. The SQA documentation relating to the course is shown here. SQA documents Web link Course specification http://bit.ly/1m3GVXu Course assessment specification http://bit.ly/1lj7sTv Course and unit support notes (the original document which has been modified in the succeeding pages) General assessment information Specimen examination paper and marking scheme http://bit.ly/1dJVNFa Course Report 2014 http://bit.ly/1EQMSyN http://bit.ly/1mskOwK http://bit.ly/1pvEOvN Education Scotland learning materials National Qualifications Glow portal http://bit.ly/1jBggj3 Past papers guidance http://bit.ly/1jqfMAF 2 NATIONAL 5 BIOLOGY RESOURCES GUIDE Cell Biology Mandatory course key areas 1 Cell structure Cell ultrastructure and functions, including cell wall, mitochondrion, chloroplast, cell membrane, cytoplasm, vacuole, nucleus, ribosome and plasmid using examples from typical plant, animal, fungi and bacterial cells. Cell wall structure in fungal and bacterial cells is different from plant cells, ie not cellulose. Unit specification: http://bit.ly/1gV5mkK Suggested learning activities Exemplification of key areas Useful resources Examine fresh and prepared slides of a range of plant, animal and microbial cells using appropriate stains and a light microscope/bioviewer, eg cheek epithelium, onion epidermis, rhubarb epidermis, Elodea, yeast. Numeracy activities on cell size to investigate cell length and breadth. Functions of structures exemplified later in this unit. Fungal structure in terms of similarity to plant and animal cells but a different cell wall structure. Bacterial structures only to include absence of organelles and a different cell wall structure to plant and fungal cells. Chemical composition of cell walls for fungi and bacteria not required. SSERC teacher resources – Cell structure BBC Knowledge and Learning revision guide – Cell structure Biology Mad learner summary and experiment – Cellular ultrastructure PhET interactive simulation – Membrane channels University of New Mexico learner summary – Cell structure and function Twig video clip – What is a cell? Twig video clip – Different types of cell Twig video clip – Pandemic viruses Twig video clip – Pandemic viruses (SARS) 3 NATIONAL 5 BIOLOGY RESOURCES GUIDE 2 Transport across cell membranes (a) The cell membrane consists of phospholipids and proteins and is selectively permeable. Highland Council resources – Transport across cell membranes (GLOW login required) http://www.educationscotland.gov.uk/nqcou rsematerials/subjects/b/nqresource_tcm47 55164.asp Investigate the structure of the fluid mosaic model. (b) Passive transport is down a concentration gradient and does not require energy. Investigate diffusion and osmosis using visking tubing and/or mass/length of plant (c) Diffusion in cells as the movement tissue, bleeding in plant cells, of molecules down a plant cell plasmolysis, mass concentration gradient. changes in egg (shell removed by soaking in vinegar) in syrup/water. (d) Osmosis in the movement of water molecules from a high water Research examples of osmosis concentration to a lower water for, for example, power concentration through a selectively permeable membrane. generation, desalination. (e) Animal cells can burst or shrink and plant cells can become turgid or plasmolysed in different solutions. (f) 4 Active transport requires energy for membrane proteins to move molecules and ions against the concentration gradient. Differential uptake of dye in boiled and live yeast cells. Different concentrations of substances exist between cells and their environment. Diffusion in terms of concentration gradients and importance to cells could include glucose, carbon dioxide, oxygen or amino acids. SSERC teacher resources – Transport across cell membranes BBC Knowledge and Learning revision guide – Transport across membranes Biology Mad learner summary – Diffusion Twig video clip – The cell membrane Appropriate examples for active transport could include sodium and potassium in nerve cells, or iodine in seaweeds. These processes can be applied across relevant areas of the course. NATIONAL 5 BIOLOGY RESOURCES GUIDE 3 Producing new cells (a) Sequence of events of mitosis (including the terms chromatids, equator and spindle fibres). (b) Maintenance of diploid chromosome complement by mitosis. (c) Mitosis is required for growth and repair. (d) Cell production by cell culture requires aseptic techniques, an appropriate medium and the control of other factors. Select and present information using mitosis stage cards. Create model chromosomes. Observe prepared root tip cell slides/bioviewer. Carry out numeracy activities based on cell growth graphs/curves. Practical activity/investigation/ research into aseptic techniques, solid and liquid media in cell culture and use of fermenters. Names of mitosis phases are not needed. SSERC teacher resources – Producing new cells Diploid cells have two matching sets of chromosomes, which are replicated during mitosis. BBC Knowledge and Learning revision guide – Producing new cells Appropriate growth media include various nutrient broths and agars. Appropriate factors could include oxygen, temperature and pH. Examples of how to achieve aseptic conditions. University of Leicester learner resource – The cell cycle, mitosis and meiosis Twig video clip – Cell division: mitosis Twig video clip – Cell division: meiosis Twig video clip – FactPack: Pregnancy timeline 5 NATIONAL 5 BIOLOGY RESOURCES GUIDE 4 DNA and the production of proteins (a) Structure of DNA: double-stranded helix held by complementary base pairs. DNA carries the genetic information for making proteins. The four bases adenine, cytosine, guanine and thymine (A, C, G and T) make up the genetic code. A is always paired with T, and C is always paired with G. The base sequence determines the amino acid sequence in protein. (b) Messenger RNA (mRNA) is a molecule that carries a complimentary copy of the code from the DNA, in the nucleus, to a ribosome, where the protein is assembled from amino acids. 6 Research the relationship between chromosomes, genes, DNA and protein to illustrate that genes are located on chromosomes. Construction of 2D or 3D DNA models. Paper models of base pairing or DNA sections. Carry out numeracy activities to determine base pair numbers. Research biologists, eg Watson Knowledge of uracil as a base in and Crick, Rosalind Franklin, mRNA is not required. Maurice Wilkins, Chargaff. Further details of transcription and translation are not required. SSERC teacher resources – DNA and the production of proteins BBC Knowledge and Learning revision guide – DNA and the production of proteins Twig video clip – How does DNA make protein? Your genome.org learner activity – Origami DNA Nobelprize.org learner information – The Discovery of the Molecular Structure of DNA: The Double Helix Teacher Tube video clip – DNA to RNA to Proteins NATIONAL 5 BIOLOGY RESOURCES GUIDE 5 Proteins and enzymes (a) The variety of protein shapes Create protein models, eg Levels of protein structure such as and functions arises from the sequence haemoglobin, antibodies, secondary/tertiary not required. of amino acids. membrane proteins and enzymes. (b) Functions of proteins to include structural, enzymes, hormones, Use of appropriate software, eg antibodies and receptors. RasMol, Protein Explorer. (c) Enzymes function as biological catalysts and are made by all living cells. They speed up cellular reactions and are unchanged in the process. The shape of the active site of enzyme molecules is complementary to a specific substrate(s). Enzyme action results in product(s). Enzymes can be involved in degradation and synthesis reactions. Examples should relate enzymes to their specific substrate(s) and product(s). SSERC teacher resources – Proteins and enzymes BBC Knowledge and Learning revision guide – Proteins and enzymes Chemguide learner resource – Protein as enzymes Twig video clip – FactPack on enzymes Twig video clip – Tropisms and hormones 7 NATIONAL 5 BIOLOGY RESOURCES GUIDE (d) Each enzyme works best in its optimum conditions. Enzymes and other proteins can be affected by temperature and pH. Enzymes can be denatured, resulting in a change in their shape, which will affect the rate of reaction. 6 Genetic engineering Genetic information can be transferred from one cell to another naturally or by genetic engineering. Stages of genetic engineering to include identifying section of DNA that contains required gene from source chromosome, extracting required gene, inserting required gene into vector/bacterial plasmid, inserting plasmid into host cell and growing modified cells to produce a genetically modified (GM) organism. Use of enzymes in this process. 8 Enzyme experiments with, for example, pepsin, lipase, amylase and catalase to investigate the influence of temperature and pH on activity. Effect of temperature and pH on egg white as a model for effect on other proteins. Research current genetic foods/issues such as golden rice, less toxic rape seed oil, bird resistance to bird flu, tomatoes with longer shelf life, blight-resistant potatoes, production of medicines for human use, eg insulin and growth hormone. DNA can be transferred naturally between cells by either bacterial plasmids or viruses. Details of these processes not required. Names of particular enzymes are not required. Links with Life on Earth unit. SSERC teacher resources – Genetic engineering BBC Knowledge and Learning revision guide – Genetic engineering Twig video clip – Genetic modification NATIONAL 5 BIOLOGY RESOURCES GUIDE 7 Photosynthesis Photosynthesis is a two-stage process. 1. Light reactions: the light energy from the sun is trapped by chlorophyll in the chloroplasts and is converted into chemical energy in the form of ATP. Water is split to produce hydrogen and oxygen. Hydrogen attaches to hydrogen acceptor molecules. Excess oxygen diffuses from the cell. 2. (a) Carbon fixation: a series of enzyme-controlled reactions that use hydrogen and ATP (produced by light reactions) with carbon dioxide to produce sugar. (b) The chemical energy in sugar is available for respiration or can be converted into other substances such as starch (storage) and cellulose (structural). Oxygen production, carbon dioxide uptake or rate of photosynthesis can be investigated through the use of floating leaf discs, Elodea, Cabomba or immobilised algae. These can also be used to investigate limiting factors. Use IT simulations of photosynthesis experiments, eg Multimedia Science Summary word equation for photosynthesis: Light energy Carbon + water → sugar +oxygen dioxide Chlorophyll SSERC teacher resources – Photosynthesis BBC Knowledge and Learning revision guide – Photosynthesis Twig video clip – Photosynthesis Virginia Tech animation – Photosynthesis: light reactions Encylopedia Britannica article – Carbon fixation and reduction Factors affecting starch production can be investigated through iodine testing in leaves. (c) Limiting factors: carbon dioxide concentration, light intensity and temperature, and their impact on photosynthesis and plant growth. Analysis of limiting factors graphs. 9 NATIONAL 5 BIOLOGY RESOURCES GUIDE 8 Respiration (a) The chemical energy stored in glucose must be released by all cells through a series of enzymecontrolled reactions called respiration. Practical investigations on burning food. Practical investigations of DCPIP/ Cabomba and hydrogen carbonate indicator. SSERC teacher resources – Respiration BBC Knowledge and Learning revision guide – Respiration Twig video clip – Respiration (b) The energy released from the breakdown of glucose is used to generate ATP from ADP and inorganic phosphate (Pi). The chemical energy stored in ATP can be released by breaking it down to ADP and inorganic phosphate. This energy can be used for cellular activities including muscle cell contraction, cell division, protein synthesis and transmission of nerve impulses. ATP can be regenerated during respiration. The breakdown of each glucose molecule via pyruvate to carbon dioxide and water in the presence of oxygen yields 38 molecules of ATP. The breakdown of each glucose molecule via the fermentation pathway yields two molecules of ATP when oxygen is not present. 10 Use immobilised yeast and hydrogen carbonate indicator, resazurin or gas sensors and data loggers to investigate rate of respiration. Cells such as muscle, companion, sperm, neurone will have a high number of mitochondria as they require a lot of energy. Twig video clip – Anaerobic respiration PBS Learning video clip – From DNA to protein Summary word equations for Royal Society of Chemistry learner Investigate respiration indirectly respiration: through breathing rates before glucose + oxygen ca rbo resource – Respiration and during exercise in humans. dioxide + water glucose e tha PhET interactive simulation – Gene carbon dioxide expression: the basics glucose la ctic a c Links with Multicellular Organisms unit. NATIONAL 5 BIOLOGY RESOURCES GUIDE Breakdown of glucose to lactic acid via pyruvate in animal cells. Breakdown of glucose to alcohol/ethanol and carbon dioxide via pyruvate in plant and yeast cells. (c) Respiration begins in the cytoplasm. The process of fermentation is completed in the cytoplasm. Aerobic respiration starts in the cytoplasm and is completed in the mitochondria. (d) Use of respirometers to measure rate of respiration. 11 NATIONAL 5 BIOLOGY RESOURCES GUIDE Multicellular Organisms Mandatory course key areas 1 Cells, tissues and organs Specialisation of cells, in animals and plants, leads to the formation of a variety of tissues and organs. Groups of organs which work together form systems. 2 Stem cells and meristems (a) Stem cells in animals can divide and have the potential to become different types of cell. Stem cells are involved in growth and repair. (b) Meristems are the sites of production of non-specialised cells in plants and are the sites for mitosis in a plant. These cells have the potential to become other types of plant cell and they contribute to plant growth. 12 Unit specification: http://bit.ly/OQJmBN Suggested learning activities Exemplification of key areas Useful resources Examine a variety of cells from different tissues to relate their structure to function. Multicellular organisms have more than SSERC teacher resources – Multicellular one cell type and are made up of organisms tissues and organs. Organs perform different functions. The cells in organs BBC Knowledge and Learning revision guide are specialised for their function. – Cells, tissues and organs Specialisation can be applied to all named tissues in this unit. Biotopics learner guide – Cell structure and specialisation Use a variety of media to investigate the potential uses of stem cells and discuss ethical issues associated with their use. SSERC teacher resources – Stem cells and meristems Carry out practicals on root tip/shoot tip stain. Twig video clip – Stem cells BBC Knowledge and Learning revision guide – Stem cells and meristems EuroStemCell teacher resources – Stem cell toolkit NATIONAL 5 BIOLOGY RESOURCES GUIDE 3 Control and communication (a) Nervous control (i) Nervous system consists of central nervous system (CNS) and nerves. CNS consists of brain and spinal cord. Structure and function of brain to include cerebrum, cerebellum and medulla. Neurons are of three types: sensory, relay and motor. Receptors detect sensory input/stimuli. Electrical impulses carry messages along neurons. A synapse occurs between neurons. Chemicals transfer these messages across synapses. (ii) Structure and function of reflex arc. (b) Hormonal control (i) Endocrine glands release hormones into the bloodstream. Hormones are chemical messengers. Target tissues have cells with receptor proteins for hormones, so only some tissues are affected by specific hormones. Investigate examples of where communication pathways are used, eg pain receptors. Internal communication is required for survival of a multicellular organism. Cells in multicellular organisms do not work independently. Sensory neurons pass the information to the CNS. The CNS processes the information from our senses which needs a response. Motor neurons enable a response to occur, which can be a rapid action from a muscle or a slower response from a gland. SSERC teacher resources – Control and communication InnerBody interactive resource – Nervous system: Anatomy explorer BBC Knowledge and Learning revision guide – Control and communication Twig video clip – The nervous system Twig video clip – Neurons as cells Twig video clip – Cortisol and chronic stress Investigate examples of human reflex activities, eg blinking, iris reflex, response to pain. Research the role of hormones in the body. Diabetes as a communication pathway that has failed due to a fault in release or a failure to respond to insulin, and consequences and treatment. Estrella Mountain Community College learner resource – The endocrine system Insulin production when blood glucose levels are higher than normal and production of glucagon when levels are below normal. 13 NATIONAL 5 BIOLOGY RESOURCES GUIDE (ii) Blood glucose regulation including the role of insulin, glucagon, glycogen, pancreas and liver. 4 Reproduction (a) The structure of gametes and the sites of their production in plants and animals. Cells are diploid, except gametes, which are haploid. Investigate the causes and treatment of both type 1 and type 2 diabetes with reference to trends in Scottish health statistics. Detail of negative feedback is not required. Compare and contrast male and female animal and plant gametes, gonads and organs from micrographs, models, reference materials, photographs and dissection of flowers. Knowledge of polyploidy organisms is not required. EndocrineWeb learner resource – Insulin regulation of blood glucose Twig video clip – Insulin and diabetes SSERC teacher resources – Reproduction BBC Knowledge and Learning revision guide – Reproduction Twig video clip – Sperm Twig video clip – Egg (b) Fertilisation is the fusion of the nuclei of the two haploid gametes to produce a diploid zygote. 14 Twig video clip – Fertilisation NATIONAL 5 BIOLOGY RESOURCES GUIDE 5 Variation and Inheritance (a) Comparison of discrete and continuous variation. (b) Most features of an individual phenotype are polygenic and show continuous variation. (c) Genetic terms including gene, allele, phenotype, genotype, dominant, recessive, homozygous, heterozygous and P1, F1 and F2. Carry out monohybrid crosses from parents through to F2. Reasons why predicted ratios are not always achieved. Investigate a variety of discrete and continuous characteristics in organisms eg ear lobes, tongue-rolling and height. Research polygenic inheritance. Combining genes from two parents contributes to variation within a species. Single gene inheritance of characters showing discrete variation where measurements fall into distinct groups. Education Scotland resources – Inheritance SSERC teacher resources – Variation and inheritance BBC Knowledge and Learning revision guide – Variation and inheritance Research Mendel’s work on peas. Continuous variation shows a range of values between a minimum and a maximum. Use Punnett squares to explain inheritance. BBC Learning Zone video clip – Dominant Family trees can be used to identify the and recessive characteristics phenotype and genotype of individuals. Times Educational Supplement learner activity – Genetic crosses worksheet (Free registration with TES connect required) YouTube screencast – Continuous and discontinuous variations Times Educational Supplement learner activity – Create a kid (Free registration with TES connect required) Twig video clip – Mendel and inheritance Twig video clip – Variation 15 NATIONAL 5 BIOLOGY RESOURCES GUIDE 6 The need for transport (a) Plant transport systems (i) Leaf structure to include upper epidermis, palisade mesophyll, spongy mesophyll, vein, lower epidermis, guard cells and stomata. Set up stomatal models, use leaf peels and microscopes to view stomata. SSERC teacher resources – The need for transport BBC Knowledge and Learning revision guide – The need for transport Twig video clip – Plant transport (ii) Other parts of the plant involved in water transport, including root hairs and xylem vessels. Water and minerals are transported in xylem vessels. Xylem vessels are dead and contain lignin for support. Water is required for transporting materials and for photosynthesis. Investigate the germination of seeds to show root hairs. (iii) The process of transpiration. Transpiration experiments to show water loss. Transpiration is the loss of water through leaves. Water is lost by evaporation through stomata, whose opening and closing is controlled by guard cells, which are found in the leaf epidermis. Mesophyll cells of the leaf require water for photosynthesis. (iv) Sugar is transported up and down the plant in living phloem cells. Structure of phloem tissue. Examine slides showing phloem structure. Details of transpiration pull and the forces involved are not required. 16 Stain xylem vessels using dye/celery. Examine slides showing xylem and phloem structure. Xylem cells are lignified to withstand the pressure changes as water moves through the plant. Biology Mad learner resources – Transport in plants NATIONAL 5 BIOLOGY RESOURCES GUIDE (b) Animal transport and exchange systems (i) In mammals, nutrients, oxygen and carbon dioxide are transported in the blood. National STEM Centre practical – Gas exchange and transport in plants and animals Twig video clip – Why is blood red? (ii) Pathway of oxygenated and deoxygenated blood through heart, lungs and body. Heart structure to include right and left atria and ventricles, and location of valves. Blood vessels to include aorta, vena cava, pulmonary arteries and veins. Coronary arteries and their function. Investigate heart structure, eg dissection, models, films. Names of valves not required. Ivy Rose diagram – The structure of the heart Twig video clip – Heart 17 NATIONAL 5 BIOLOGY RESOURCES GUIDE (iii) Arteries have thick muscular walls, a narrow central channel and carry blood under high pressure away from the heart. Veins carry blood under low pressure, have thinner walls and a wide channel. Veins contain valves to prevent backflow of blood and carry blood towards the heart. Capillaries form networks at organs and tissues, are thin walled and have a large surface area, allowing exchange of materials. (iv) Red blood cells are specialised by being biconcave in shape, having no nucleus and containing haemoglobin. This allows them to transport oxygen efficiently in the form of oxyhaemoglobin. 18 Use of diagrams/models to illustrate the structure of arteries, veins, capillaries and blood cells. InnerBody interactive resource – Crosssection of artery and vein Medline Plus video clip – Red blood cell production Royal Society of Chemistry teacher resource – Transport of oxygen in the blood NATIONAL 5 BIOLOGY RESOURCES GUIDE (v) Rings of cartilage keep main airways open. Oxygen and carbon dioxide are exchanged through the alveolar walls. Alveoli have a large surface area, thin walls and a good blood supply for more efficient diffusion of gases. Mucus traps dirt and microorganisms, and cilia moves this up and away from the lungs. Investigate lungs, eg dissection, model, films. (vi) Food is moved through the digestive system by peristalsis. Villi in the small intestine have a large surface area, thin walls and a good blood supply to aid absorption of glucose and amino acids. The lacteals absorb fatty acids and glycerol (the products of fat digestion). Peristalsis model. Model gut. BBC Learning Zone video clip – Anatomy and physiology of the lungs Comparisons can be made between cartilage in airways and lignin in xylem. Penn University animation – Peristalsis 19 NATIONAL 5 BIOLOGY RESOURCES GUIDE 7 Effects of lifestyle choices on human transport and exchange systems Use health promotion and reference materials to identify how healthier lifestyle choices can directly and indirectly improve the physical and mental health of an individual. Take physiological measurements. Investigate the effect of moderate exercise on these measurements. Possible examples of lifestyle choices could be high-fat or high-salt diet, lack of exercise, use of tobacco or alcohol, or high-stress experiences. These directly and indirectly increase the chances of fatty deposits in blood vessels, blood clots, heart attacks, strokes, diabetes and stress. Education Scotland resources – Health and disease (Glow login required) SSERC teacher resources – Effects of lifestyle choices on human transport and exchange systems PhET interactive simulation – Eating and exercise Twig video clip – Balanced diet Twig video clip – Alcohol and the brain Twig video clip – What’s in a cigarette? Twig video clip – Obesity 20 NATIONAL 5 BIOLOGY RESOURCES GUIDE Life on Earth Unit specification: http://bit.ly/1m3HxMQ Mandatory course key areas Suggested learning activities Exemplification of key areas Useful resources 1 Biodiversity and the distribution of life (a) Biotic and abiotic factors affect biodiversity in an ecosystem. Human activities can also have an impact on biodiversity. Investigate a range of human influences that affect national and global environments, such as pollution of air and water, habitat destruction, eg by deforestation (tropical rain forest), desertification, endangered species, overfishing and lichen studies. Various factors can increase or decrease the biodiversity of an ecosystem. SSERC teacher resources – Biodiversity and the distribution of life (b) Competition for resources, disease, food, grazing and predation are biotic factors. Light intensity, moisture, pH and temperature are abiotic factors. (c) Biomes are the various regions of our planet as distinguished by their climate, fauna and flora. Global distribution of biomes can be influenced by temperature and rainfall. BBC Knowledge and Learning revision guide – Biodiversity and the distribution of life Twig video clip – What is an ecosystem? Twig video clip – Oceans: The abyss Interpret predator–prey interaction graphs. Analyse barn owl pellets from Barn Owl Trust website. Research a range of biomes, eg marine, tundra, prairie, polar, temperate deciduous forest and tropical rain forest. Twig video clip – Symbiosis: Parasitism Barn Owl Trust learner activity – Barn owl pellets Names of individual biomes are not required. Missouri Botanical Garden learner information – What’s it like where you live? Enchanted Learning learning resources – Biomes: Habitats 21 NATIONAL 5 BIOLOGY RESOURCES GUIDE (d) An ecosystem consists of all the organisms (the community) living in a particular habitat and the non-living components with which the organisms interact. Case studies/fieldwork on, for example, Caledonian forests, sea lochs, heather moorland, tropical rainforests, Arctic tundra, coral reefs and deserts. (e) A niche is the role that an organism plays within a community. It includes the use the organism makes of the resources in its ecosystem, including light, temperature and nutrients, and its interactions with other organisms in the community, including competition, parasitism and predation. Investigate examples of niches of Scottish wildlife, eg wildcat, red squirrel, red grouse, Scottish crossbill, brown trout, and bracken. Analyse data related to distribution of barnacles on rocky shores, native woodland and red deer numbers, and distribution of ptarmigan. 22 Red squirrel.org learner information – Red squirrels in south Scotland NATIONAL 5 BIOLOGY RESOURCES GUIDE 2 Energy in ecosystems (a) Definitions of other ecological terms, including species, population, producer, consumer, herbivore, carnivore and omnivore. (b) In transfers from one level to the next in a food chain, 90% of energy is lost as heat, movement or undigested materials. 10% is used for growth. (c) Definitions and comparisons of pyramids of biomass, energy and numbers. (d) Competition in ecosystems. Interspecific competition occurs when individuals of different species require similar resources in an ecosystem. Intraspecific competition occurs when individuals of the same SSERC teacher resources – Energy in ecosystems BBC Knowledge and Learning revision guide – Energy in ecosystems Investigate examples of pyramid of energy (as measured in kJ/m2/year), pyramid of biomass (g/m2) and pyramid of numbers. Investigate irregular pyramids of number, eg a tree as a producer, presence of parasites. Irregular shapes of pyramids of numbers based on different body sizes are represented as true pyramids of energy and of biomass. Examples of competition can include the need for food, light and water. Investigate interspecific competition, eg red and grey squirrels, brown and rainbow trout. Investigate intraspecific competition, eg cress seedling density, trees of the same species growing close together, feeding in grasshoppers, territorial behaviour in birds, eg robins, red grouse. National STEM Centre interactive resource – Energy transfer in ecosystems Times Educational Supplement worksheet – Pyramids of number and biomass (TES Connect free registration required) BBC Knowledge and Learning video clip – Ecosystems and species diversity Forestry Commission learner information – Habitat use by red and grey squirrels The Fuse School animation – Interspecific and intraspecific interactions Competition examples can include food, light and water. Times Educational Supplement learner activity – Nitrogen cycle construction activity (TES Connect free registration required) 23 NATIONAL 5 BIOLOGY RESOURCES GUIDE species require the same resources. Intraspecific competition is therefore more intense than interspecific competition. (e) Nitrogen in ecosystems Plant proteins are produced using nitrogen from nitrates. The roles of nitrifying, denitrifying, root nodule and free-living nitrogen-fixing soil bacteria. Decomposers, such as fungi and bacteria, convert proteins and nitrogenous wastes to ammonium compounds These are converted to nitrites and then to nitrates. Animals obtain the nitrogen required to produce protein by consuming plants. 24 YouTube animation – Nitrogen cycle YouTube video clip – Nitrogen cycle 1 Construct simple flow diagrams that illustrate the cyclical activities in the nitrogen cycle. Fertilisers supply nitrates to increase yield. NATIONAL 5 BIOLOGY RESOURCES GUIDE 3 Sampling techniques and measurement of abiotic and biotic factors (a) Sampling of plants and animals using techniques including quadrats and pitfall traps. Techniques for biotic factors, eg quadrats, transect, pitfall trap, Tullgren funnel, pooters, tree beating/sweep net, pond netting. Representative sampling and adequate Education Garden learner resource – replication. Investigating an ecosystem (b) Evaluation of limitations and sources of error in the use of quadrats and pitfall traps. BBC Knowledge and Learning revision guide – Sampling techniques and measurement of abiotic and biotic factors (c) Using and constructing paired-statement keys to identify organisms. (d) Measuring abiotic factors, including light intensity, temperature, pH and soil moisture. Possible sources of error and how to minimise them. (e) The effect of abiotic factors on the distribution of organisms. SSERC teacher resources – Sampling techniques and measurement of abiotic and biotic factors SAPS teacher resource – Making and using keys Techniques for abiotic factors: temperature using thermometer or temperature probes, light using light meters, moisture using moisture meters, pH using pH meters or chemical test. Use of probes linked to appropriate data-logging software. YouTube video clip – Abiotic and biotic factors YouTube video clip – Measuring abiotic components of the system 25 NATIONAL 5 BIOLOGY RESOURCES GUIDE 4 Adaptation, natural selection and the evolution of species (a) A mutation is a random change to genetic material. Mutations may be neutral or confer an advantage or a disadvantage. Mutations are spontaneous and are the only source of new alleles. Environmental factors, such as radiation, high temperatures and some chemicals, can increase the rate of mutation. (b) New allelles produced by mutation allow plants and animals to adapt to their environment. These adaptations can be structural or behavioural. Variation within a population makes it possible for a population to evolve over time in response to changing environmental conditions. 26 Research different types of mutation: neutral, advantageous or disadvantageous. SSERC teacher resources – Adaptation, natural selection and the evolution of species Research mutagenic agents. BBC Knowledge and Learning revision guide – Adaptation, natural selection and the evolution of species Twig mindmap – Adaptation Twig mindmap – Extinction Twig mindmap – Evolution of man Investigate examples of adaptations such as desert mammals and plants, and Galapagos finches. An adaptation is an inherited characteristic that makes an organism well suited to survival in its environment/niche. Twig video clip – Adaptation Twig video clip – Natural selection Twig video clip – Mechanisms of evolution Twig video clip – Evolution: the evidence Twig video clip – Origin of species NATIONAL 5 BIOLOGY RESOURCES GUIDE (c) Species produce more offspring than the environment can sustain. Natural selection or survival of the fittest occurs when there are selection pressures. The best-adapted individuals survive to reproduce, passing on the favourable alleles that confer the selective advantage. Research consequences of overprescription of antibiotics. Case study on insect resistance to ‘built-in’ insecticides in GM crops, eg bollworm moth in cotton, European corn borer in maize. (d) Speciation occurs after part of a population becomes isolated by an isolation barrier. This can be geographical, ecological or reproductive. Mutations occur in each sub-population. Natural selection selects for different mutations in each group due to different selection pressures. Each sub-population evolves until they become so genetically different they are two different species. Examples of types of barriers are Research Scottish examples of isolation leading to speciation, eg acceptable, eg river, mountain. Arran Whitebeam, St Kilda Wren, Arctic Char. Research examples of rapid natural selection, eg MRSA, insect resistance to GM crop toxins. BBC Learning Zone video clip – Natural selection and survival of the fittest PhET interactive simulation – Natural selection BBC Learning Zone video clip – Variation 27 NATIONAL 5 BIOLOGY RESOURCES GUIDE 5 Human impact on the environment (a) Increasing human population requires an increased food yield. Research GM crops, monoculture and intensive farming. There are opportunities throughout this topic for learners to investigate and debate ethical issues. (b) Fertilisers can leach into fresh water, increasing algal blooms. This reduces light levels, killing aquatic plants. These dead plants as well as dead algae become the food for bacteria, which increase greatly in number. The bacteria use up large quantities of oxygen, reducing the oxygen availability for other organisms. (c) Indicator species are species that by their presence or absence indicate environmental quality/levels of pollution. (d) Pesticides sprayed onto crops can accumulate in 28 SSERC teacher resources – Human impact on the environment BBC Knowledge and Learning revision guide – Human impact on the environment Twig video clip – Algae Survey local area for different varieties of lichen and assess local air quality. Sample freshwater invertebrates from local water courses/ponds and assess water quality. Freshwater Biological Association activities – Collecting freshwater macroinvertebrate samples Twig video clip – Lichen indicator species BBC GCSE Bitesize revision guide – Bioaccumulation and eutrophication: Higher tier NATIONAL 5 BIOLOGY RESOURCES GUIDE the bodies of organisms over time. As they are passed along food chains, toxicity increases and can reach lethal levels. (e) Biological control may be an alternative to the use of pesticides. GM crops may be an alternative to the use of fertilisers. Investigate bioaccumulation, eg DDT. James Hutton Institute pesticides game – You choose the news Royal Horticultural Society learner information – Biological pest control Investigate biological control, eg using a virus (myxomatosis) to kill rabbits, using ladybirds to kill aphids and scale insects, using caterpillar moth (Cactoblastis) to kill cacti (Opuntia). Investigate GM rice plants that take up nitrogen more efficiently. 29