Product datasheet
Anti-beta 2 Adrenergic Receptor antibody ab61778
3 Abreviews 4 Images
Overview
Product name
Anti-beta 2 Adrenergic Receptor antibody
Description
Rabbit polyclonal to beta 2 Adrenergic Receptor
Specificity
ab61778 detects endogenous levels of total beta 2 Adrenergic Receptor protein.
Tested applications
WB, ELISA, ICC/IF, IHC-P, Flow Cyt
Species reactivity
Reacts with: Human
Immunogen
Synthetic non-phosphopeptide derived from human beta 2 Adrenergic Receptor around the
phosphorylation site of serine 346.
Positive control
Human brain tissue. HeLa cells.
General notes
Based on immunogen sequence homology data ab61778 is predicted to react with mouse
species however customers has provided us mixed feedback which is why we no
longer support its cross reactivity with mouse samples.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Purity
Immunogen affinity purified
Purification notes
ab61778 was affinity-purified from rabbit antiserum by affinity-chromatography using epitopespecific immunogen.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab61778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/1000. Predicted molecular weight: 46 kDa. (see Abreview)
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Application
Abreviews
Notes
ELISA
1/5000.
ICC/IF
1/500 - 1/1000.
IHC-P
1/50 - 1/100.
Flow Cyt
1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control
with this antibody.
Target
Function
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase
through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an
approximately 30-fold greater affinity than it does norepinephrine.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 subsubfamily.
Post-translational
modifications
Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the
plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345
and Ser-346 phosphorylation.
Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous
desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate
phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the
lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling
and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively
associated and are dissociated immediately after agonist stimulation.
Cellular localization
Cell membrane.
Anti-beta 2 Adrenergic Receptor antibody images
ab61778, at 1/50 - 1/100 dilution, staining
beta 2 Adrenergic Receptor in paraffinembedded human brain tissue by
immunohistochemistry, in the absence (left
panel) or presence (right panel) of the
immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - beta 2 Adrenergic
Receptor antibody (ab61778)
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ab61778, at 1/500 - 1/1000 dilution, staining
beta 2 Adrenergic Receptor in HeLa cells by
immunofluorescence, in the absence (left
panel) or presence (right panel) of the
immunising peptide.
Immunocytochemistry/ Immunofluorescence beta 2 Adrenergic Receptor antibody (ab61778)
ab61778 staining the beta 2 adrenergic
receptor in Human peripheral blood
monomuclear cells by Flow Cytometry. Cells
was prepared by Ficoll gradient separation in
PBS/BSA and was fixed with
paraformaldehyde. The sample was
incubated with the primary antibody (1/50 in
PBS + 0.5% BSA) for 1 hour at 18°C.
Ab7007 (1/100) was used as secondary
antibody.
Gating Strategy: Monocytes
Flow Cytometry - beta 2 Adrenergic Receptor
antibody (ab61778)
This image is courtesy of an anonymous Abreview
All lanes : Anti-beta 2 Adrenergic Receptor
antibody (ab61778)
Lane 1 : NIH-3T3 cell lysate.
Lane 2 : NIH-3T3 cell lysate with blocking
peptide.
Predicted band size : 46 kDa
Observed band size : 48 kDa
Western blot - beta 2 Adrenergic Receptor
antibody (ab61778)
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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