Product datasheet 11-dehydro-TXB2 ELISA Kit ab133054 1 Image Overview Product name 11-dehydro-TXB2 ELISA Kit Detection method Colorimetric Precision Intra-assay Sample n Mean Low conc 16 67pg/ml 13.2% High conc 16 318pg/ml 8.1% Sample type Cell culture supernatant, Urine Assay type Competitive Sensitivity 4.31 pg/ml Range 9.8 pg/ml - 10000 pg/ml SD CV% Recovery Sample specific recovery Sample type Average % Range Urine 112.2 %-% Tissue Culture Media 104.8 %-% Assay time 3h 0m Assay duration Multiple steps standard assay Species reactivity Reacts with: Human Predicted to work with: a wide range of other species Product overview Abcam’s 11-dehydro-TXB2 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 11-dehydro-TXB2 in tissue culture media and urine. A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-11dehydro-TXB2 antigen and a polyclonal rabbit antibody specific to 11-dehydro-TXB2. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 11-dehydro-TXB2 captured in the plate. 1 Notes 11-dehydro-Thromboxane B2 (11-dehydro-TXB2) is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma. It is produced by the dehydrogenation of the alcohol group on the C11 position of TXB2 by the enzyme 11-OH-dehydrogenase. The detection of 11-dehydroTXB2 is often used to measure thromboxane production in vivo. Monitoring levels of this metabolite is a helpful tool in the study of several diseases, such as liver cirrhosis, cystic fibrosis, mastocytosis, systemic lupus erythematosus, thrombosis diseases, and other diseases involving platelet activation.11-dehydro-TXB2 has also been used in diabetes and asthma studies. Cross reactivities: Compound Cross Reactivity 11-dehydro-TXB2100% PGE2 1.85% TXB2 0.4% PGF2α 0.2% 6-keto-PGF1α 0.17% PGD2 0.1% 2,3-dinor TXB2 0.1% 8-iso-PGF2α 0.07% 11-b-PGF2α 0.05% Tested applications Competitive ELISA Platform Microplate Properties Storage instructions Please refer to protocols. Components 1 x 96 tests 11-dehydro-Thromboxane B2 Standards 1 x 500µl 11-dehydro-TXB2 Alkaline Phosphatase Conjugate 1 x 6ml 11-dehydro-TXB2 Antibody 1 x 6ml 20X Wash Buffer Concentrate 1 x 30ml Assay Buffer 1 x 30ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml Applications 2 Our Abpromise guarantee covers the use of ab133054 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Competitive ELISA Notes Use at an assay dependent concentration. 11-dehydro-TXB2 ELISA Kit images Representative Standard Curve using ab133054. Typcial Standard Curve Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3