Product datasheet
Substance P ELISA Kit ab133029
2 Images
Overview
Product name
Substance P ELISA Kit
Detection method
Colorimetric
Precision
Intra-assay
Sample
n
Mean
SD
CV%
Low conc
24
101pg/ml
6.7%
Medium conc
24
1116pg/ml
4.5%
High conc
24
6257pg/ml
5.2%
Inter-assay
Sample
n
Mean
SD
CV%
Medium conc
8
1120pg/ml
7.3%
High conc
8
6402pg/ml
7.3%
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Plasma
Assay type
Competitive
Sensitivity
8.04 pg/ml
Range
9.8 pg/ml - 10000 pg/ml
Recovery
Sample specific recovery
Sample type
Average %
Range
Saliva
108.3
%-%
Urine
105.8
%-%
Serum
97.7
%-%
Tissue Culture Media
81
%-%
Heparin Plasma
109.3
%-%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human, Pig
Predicted to work with: a wide range of other species
Product overview
Abcam’s Substance P in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is
designed for the accurate quantitative measurement of Substance P in biological fluids.
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A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test
samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Substance
P antigen and a polyclonal rabbit antibody specific to Substance P. After incubation the excess
reagents are washed away. pNpp substrate is added and after a short incubation the enzyme
reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow
coloration is inversely proportional to the amount of Substance P captured in the plate.
Notes
Substance P is a undecapeptide that displays a number of biological activities. The peptide was
first discovered in 1931 by von Euler and Gaddum. They reported that extracts of equine brain
and intestine contained a hypotensive and spasmogenic factor. The preparation, termed
preparation P, was later found to be proteinaceous. The isolation and characterization of
Substance P was carried out by Leeman’s group in 1970. The structure is shown below.
Substance P: H-Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2
Substance P is synthesized in the ribosomes as a larger protein and then enzymatically
converted into the active peptide. The peptide is widely distributed in the peripheral and central
nervous systems of vertebrates, where it is thought to act as a neurotransmitter. In the peripheral
system, Substance P is localized in the primary sensory neurons and neurons intrinsic to the
gastrointestinal tract.
Cross Reactivity
Compound
% Cross Reactivity
Substance P
100
Substance P (3-11)
85.9
Physalaemin
75.3
Substance P (4-11)
11.7
Substance P (7-11)
5.9
α-Neurokinin
0.8
β-Neurokinin
0.2
Somatostatin
<0.001
Substance P (1-4)
<0.001
Tested applications
Competitive ELISA
Platform
Microplate
Properties
Storage instructions
Please refer to protocols.
Components
1 x 96 tests
20X Wash Buffer Concentrate
1 x 30ml
Assay Buffer
1 x 30ml
Goat anti-rabbit IgG Microplate (12 x 8 wells)
1 unit
Plate Sealer
1 unit
pNpp Substrate
1 x 20ml
Stop Solution
1 x 5ml
Substance P Alkaline Phosphatase Conjugate
1 x 6ml
Substance P Antibody
1 x 6ml
Substance P Standard
1 x 500µl
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Applications
Our Abpromise guarantee covers the use of ab133029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Competitive ELISA
Notes
Use at an assay dependent concentration.
Substance P ELISA Kit images
Substance P measured in human, rat and pig
biological fluids; background signal
subtracted (duplicates +/- SD).
Competitive ELISA - Substance P ELISA Kit
(ab133029)
Representative Standard Curve using
ab133029.
Typical Standard Curve
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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