Product datasheet
Anti-Calcium binding protein P22 antibody - Nterminal ab196807
1 Image
Overview
Product name
Anti-Calcium binding protein P22 antibody - N-terminal
Description
Rabbit polyclonal to Calcium binding protein P22 - N-terminal
Tested applications
WB
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen
Synthetic peptide corresponding to Human Calcium binding protein P22 (N terminal).
Database link: Q99653
Positive control
HeLa cell extract
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab196807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
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Target
Function
Required for constitutive membrane traffic. Inhibits GTPase-stimulated Na(+)/H(+) exchange.
Also inhibits calcineurin phosphatase activity. Required for activity of SLC9A1/NHE1.
Tissue specificity
Ubiquitously expressed. Has been found in fetal eye, lung, liver, muscle, heart, kidney, thymus
and spleen.
Sequence similarities
Contains 4 EF-hand domains.
Post-translational
modifications
Both N-myristoylation and calcium-mediated conformational changes are essential for its
function in exocytic traffic.
Phosphorylated; decreased phosphorylation is associated with an increase in exchange activity.
The phosphorylation state may regulate the binding to NHE1.
Cellular localization
Cytoplasm.
Anti-Calcium binding protein P22 antibody - N-terminal images
All lanes : Anti-Calcium binding protein P22
antibody - N-terminal (ab196807) at 1/500
dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with synthesized
peptide
Predicted band size : 23 kDa
Western blot - Anti-Calcium binding protein P22
antibody - N-terminal (ab196807)
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