Elisabeth Huff-Lonergan Degrees Held Ph.D. Muscle Biology and Meat Science, Minor in Biochemistry Iowa State University 1995 M.S. Meat Science Iowa State University 1991 B.S. Food Science and Nutrition University of Missouri/Columbia 1988 Cum Laude Summary of Professional Experience July 2008-Present - Iowa State University, Ames, Iowa, Professor, Meat Science and Muscle Biology, Animal Science Department. Appointment: 75% Research, 25% Teaching (20082012) Current -50% Research, 25% Teaching, 25% Administration (Administration as Faculty fellow- 2012-2015) July 2004 – June 2008 - Iowa State University, Ames, Iowa, Associate Professor, Meat Science and Muscle Biology, Animal Science Department. Appointment: 75% Research, 25% Teaching Oct. 1998 – June 2004 - Iowa State University, Ames, Iowa, Assistant Professor, Meat Science and Muscle Biology, Animal Science Department. Appointment: 75% Research, 25% Teaching 1995 to 1998 - Auburn University, Auburn, Alabama, Assistant Professor, Meat Science and Muscle Biology. Department of Animal and Dairy Sciences. Appointment: 70% research, 30% teaching 1989 to 1995 - Iowa State University, Ames, Iowa, Research Assistant, Animal Science Department. 1989 to 1994 - Iowa State University, Ames, Iowa, Teaching Assistant, Animal Science Department ISU Personnel Record Member of the Emerging Leaders Academy 2013-2014 ADVANCE Faculty Fellow – Iowa State University – 2012-2015. In charge of University-wide initiatives to assist in the recruitment and retention of women and under-represented minorities in the faculty ranks on campus. 25% Appointment in Provost’s Office ADVANCE Professor for the Department of Animal Science-August 2008-10 (NSF funded initiative) – responsible for facilitating the identification and implementation of new policies and practices to enhance departmental communication and workplace satisfaction and to work with College and University level administrators/researchers in evaluating the effectiveness of the changes. Member of College of Agriculture and Life Sciences Promotion and Tenure Committee – 2010-2012 Chair – Fall 2012 Member of the Animal Science Graduate Faculty Council Member of the Animal Science Undergraduate Advising group Member of the Animal Science Graduate Faculty Member of Meat Science Faculty Group Member of Muscle Biology Faculty Group 2 Professional Affiliations and Activities American Meat Science Association – Member – 1989-present Publication Task force – Chair – 2006-2008; member 2006-presnet Board of Directors – Member – 2004-2006 Research Award Committee – 2001-2003 Achievement Award Committee Chairman, 2003 International Relations Committee Member, 2002 Graduate Student Poster Committee - Chairman - 1997 Coordinated the Graduate Student Poster Competition (Master’s and Ph.D. level) at the Association’s Annual Meeting Annual Meeting Program Committee-1996-1997 - Carcass Composition/Fresh Meat – Member Annual Meeting Program Committee-1995-1996 - Biochemistry Committee – Member Annual Meeting Program Committee-1993-1994 - Graduate Student Programs Committee - Member Annual Meeting Program Committee-1992-1993 - Tenderness and Textural Analysis Invited Poster Committee – Member Annual Meeting Program Committee-1990-1991 - Muscle Biochemistry Committee – Member American Society of Animal Science – Member – 1989-present Board of Directors for the American Society of Animal Science – Member, 2013-2016 Publications Committee – Chair, 2013-2016 International Committee – Chair, 2013-2016 Cell Biology Symposium Committee – Member 2007-2009 Meat Science and Muscle Biology Program Committee Member, 2002-2004 Chair 2005 – Organized a symposium “Novel Technologies in Muscle Biology/Fresh Meat Research” Meats Research Award Committee, 2000 – 2003, 2011-12 Midwest Section of the American Society of Animal Science – Member – 1989-present Academic Quadrathlon Committee, 2001-2004. Institute of Food Technologists – Member – 1988-present Muscle Foods Division – Member Muscle Foods Division Committee Member-at-Large. 1999-2001 Iowa Section – Member Sigma Xi – Member –1992-2004 Elisabeth Huff Lonergan 3 Scholarship Awards Iowa State University College of Agriculture and Life Sciences Diversity Award – 2015. Signal Service Award –American Meat Science Association – 2014. This award is the Fellow award for the American Meat Science Association. Given to those members who over their careers have made a significant impact on the association and on the discipline both nationally and internationally. Institute of Food Technologists (IFT) Fellow – 2013 The award is given to only the top 0.3% (maximum) of the membership. There are currently over 4000 professional members in IFT Iowa State University – University Award for Mid-Career Research Achievement - 2010 American Meat Science Association – Distinguished Research Award – Highest research focused award this international society gives. (first woman to be awarded this recognition) - 2009 American Society of Animal Science – Meat Science Research Award (International Career Award) – July 2007 (first woman to be awarded this recognition) American Society of Animal Science. –Outstanding Young Scientist Award in Research, Midwestern Section – 2005 American Meat Science Association - Achievement Award, 2002 Iowa State University College of Agriculture - Early Achievement in Research Award, 2001 Professional Recognitions/Key Society Service Appointments Meat Science (Elsevier Press – and official journal of the American Meat Science Association) – Associate Editor. Handle approximately 300 articles per year. 2008-2014 Animal Frontiers – (International Animal Science Review/Current Events Journal) member of the management board 2013-2015 Animal Frontiers – (International Animal Science Review/Current Events Journal) Invited Guest Editor (with Steven Lonergan) for the October 2012 issue on meat. FASEB Summer Conference – Biology of Calpains in Health and Disease. Invited to chair the session titled “Calpains in Muscle” –held July 21-26, 2013 Journal of Animal Science – Editorial Board Member, 1997-2000, 2008-2010 Fleischwirtschaft – Editorial Board Member – 2008-2012 Elisabeth Huff Lonergan 4 Journal of Muscle Foods – Editorial Board Member, 2000-2011 FASEB Summer Conference – Biology of Calpains in Health and Disease. Invited to chair the session titled “Calpains in Animal Science –held July14-19, 2007 3 x USDA/NRI Grant Review Panel Member – Panel 71.1 Improving Food Quality and Value – 2005, 2007, 2011 Iowa State University Graduate Council – Elected as Biological and Agricultural Sciences representative – 2006-2009. American Meat Science Association – Elected to serve on the Board of Directors 2004-2006 Cold Spring Harbor Labs – Selected to be one of 16 participants in a 2 week, intensive workshop on cutting-edge technologies in Proteomics. Selected from a pool of over 200 applicants world-wide in 2004 Elisabeth Huff Lonergan 5 Publications and Research Presentations Cited over 2520 times, cited over 2200 times without self citations, average citations per article is 13.63, h-index = 24: Source – ISI Web of Science, Accessed January 12, 2015) Peer-Reviewed Publications 1. Huff, E.J., and F.C. Parrish, Jr. 1993. Bovine longissimus muscle tenderness as affected by postmortem aging time, animal age and sex. J. Food Sci. 58:713-716. (approximate percentage contribution – 70%). Elisabeth Huff Lonergan was responsible for designing the study, collecting the data, writing the manuscript and responding to reviewers’ comments. 2. Huff-Lonergan, E., F.C. Parrish, Jr., and R.M. Robson. 1995. Effects of postmortem aging time, animal age, and sex on degradation of titin and nebulin in bovine longissimus muscle. J. Anim. Sci. 73:1064-1073. (approximate percentage contribution – 70%). Elisabeth Huff Lonergan was responsible for designing the study, collecting the data, writing the manuscript and responding to reviewers’ comments. In June of 2007 this paper was the most frequently read meat science article in the Journal of Animal Science and was number 12 overall. 3. Huff-Lonergan, E., T. Mitsuhashi, D.D. Beekman, F.C. Parrish, Jr., D.G. Olson, and R.M. Robson. 1996. Proteolysis of specific muscle structural proteins by µ-calpain at low pH and temperature is similar to degradation in postmortem bovine muscle. J. Anim. Sci. 74:993-1008. (approximate percentage contribution – 70%). Elisabeth Huff Lonergan was responsible for designing the study, collecting the data, writing the manuscript and responding to reviewers’ comments. In February of 2007 this paper was the most frequently read meat science article in the Journal of Animal Science. In July of 2007 this paper was in the top 50 most cited papers in the Journal of Animal Science across all subjects (rank = 47). 4. Huff-Lonergan, E., T. Mitsuhashi, F.C. Parrish, Jr., and R.M. Robson. 1996. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting comparisons of purified myofibrils and whole muscle preparations for evaluating titin and nebulin in postmortem bovine muscle. (approximate percentage contribution – 70%). J. Anim. Sci. 74:779-785. Elisabeth Huff Lonergan was responsible for designing the study, collecting the data, writing the manuscript and responding to reviewers’ comments. 5. Montgomery, J.L., F.C. Parrish, Jr., D.C. Beitz, R.L. Horst, E.J. Huff-Lonergan, and A.H. Trenkle. 2000. The use of vitamin D3 to improve beef tenderness. J. Anim. Sci. 78:2615-2621. (approximate percentage contribution – 30%). This paper reports work done by Jayden Montgomery for his Ph.D. under the direction of F.C. Parrish, Jr. Dr. Huff Lonergan was responsible for designing the methods for collecting the protein biochemistry data and for directing the collection and interpretation of the protein biochemistry data. 6. Harris, S.E., E. Huff-Lonergan, S.M. Lonergan, W.R. Jones, and D. Rankins. 2001. Antioxidant status affects color stability and tenderness of calcium chloride injected beef. J. Anim. Sci. 79:666677. (approximate percentage contribution – 50%). This paper reports the work done by Scott Harris for his Master of Science degree under the direction of Elisabeth Huff Lonergan. Scott received his degree in the Spring of 1999. Dr. Huff Lonergan was responsible for obtaining the funding (grant # 4) and for directing the design, collection and interpretation of the data and overseeing the writing of the paper. Dr. Huff Lonergan served as the senior author. 7. Lonergan, S.M., E. Huff-Lonergan, L.J.Rowe, D.L. Kuhlers, and S.B. Jungst. 2001. Selection for lean growth efficiency in Duroc pigs: Influence on pork quality. J. Anim. Sci. 79:2075-2085. Elisabeth Huff Lonergan 6 (approximate percentage contribution – 40%). Dr. Huff Lonergan was responsible for obtaining funding for the project (grant # 6) and for collecting and interpreting the protein degradation data. Dr. Huff Lonergan also served as senior author on the paper. 8. Lonergan, S.M., E. Huff-Lonergan, B.R. Wiegand, and L.A. Kriese-Anderson. 2001. Postmortem proteolysis and tenderization of top loin steaks from Brangus cattle. J. Muscle Foods 12:121-136. (approximate percentage contribution – 30%).This paper reports work done with beef quantitative geneticist Lisa Kreise-Anderson at Auburn University. Dr. Huff Lonergan contributed to the development of the proposals that funded the work (grants # 1 and 7) and aided in collection and interpretation of the protein biochemistry data. 9. Malek, M., J.C.M. Dekkers, H.K. Lee, T.J. Baas, K. Prusa, E. Huff-Lonergan, and M. Rothschild. 2001. A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. II. Meat and muscle composition. Mammalian Genome 12:637-645. (approximate percentage contribution – 20%). This paper reports work done as a collaborative effort with the Iowa State University animal breeding and genetics group. Dr. Huff Lonergan collected and interpreted the protein biochemistry data. 10. Wiegand, B.R., F.C. Parrish, Jr., D.G. Morrical, and E. Huff-Lonergan. 2001. Feeding high levels of vitamin D3 does not improve the tenderness of callipyge lamb loin chops. J. Anim. Sci. 79:2086-2091. (approximate percentage contribution – 30%). This paper reports work done by Bryon Wiegand while working on his Ph.D. at Iowa State University under the guidance of F.C. Parrish, Jr. Dr. Huff Lonergan was involved in directing the collection and interpretation of the protein biochemistry data in the paper. 11. Chiba, L.I., D.L. Kuhlers, L.T. Frobish, S.B. Jungst, E.J. Huff-Lonergan, S.M. Lonergan, and K.A. Cummins. 2002. Effect of dietary restrictions on growth performance and carcass quality of pigs selected for lean growth efficiency. Livest. Prod. Sci. 74:93-102 (approximate percentage contribution – 40%). This paper reports work done in collaboration with swine nutritionists and swine quantitative geneticists at Auburn University. Dr. Huff Lonergan was involved in obtaining partial funding for the project (grant #6), collection of data and in interpretation of pork quality data. 12. Huff-Lonergan, E., T.J. Baas, M. Malek, J. Dekkers, K. Prusa, and M.F. Rothschild. 2002. Correlations among selected pork quality traits. J. Anim. Sci. 80:617-627. (approximate percentage contribution – 40%). This paper reports work done as a collaborative effort with the Iowa State University animal breeding and genetics group. Dr. Huff Lonergan collected and interpreted the protein biochemistry data, wrote the paper and served as senior author on the paper. 13. Helman, E.E., E. Huff-Lonergan, G.M. Davenport, and S.M. Lonergan. 2003. Effect of dietary protein on calpastatin in canine skeletal muscle. J. Anim. Sci. 81:2199-2205. (approximate percentage contribution – 40%). This paper reports work done by Emily Helman for her Master of Science degree that she completed in the spring of 2001 under the direction of Steven Lonergan. Dr. Huff Lonergan was responsible for obtaining the funding for the projcect (grant # 9) and aided in interpretation of the data. 14. Davis, K.J., J.G. Sebranek, E. Huff-Lonergan, and S.M. Lonergan. 2004. The effects of aging on moisture-enhanced injected pork loins. Meat Science. 66:519-524. (approximate percentage contribution – 40%) This paper reports some of the work done by Kathy Davis for her Master of Science degree. Kathy was co-advised by Elisabeth Huff-Lonergan and Joe Sebranek and graduated in December of 2001. Dr. Huff Lonergan played a significant role in assisting the development of the proposal (grant # 15) and interpreting some of the data for the paper. Elisabeth Huff Lonergan 7 15. Davis, K.J., J.G. Sebranek, E. Huff-Lonergan, and S.M. Lonergan. 2004. The effects of irradiation on quality of injected fresh pork loins. Meat Science. 67:395-401. (approximate percentage contribution – 40%). This paper reports some of the work done by Kathy Davis for her Master of Science degree. Kathy was co-advised by Elisabeth Huff-Lonergan and Joe Sebranek and graduated in December of 2001. Dr. Huff Lonergan played a significant role in assisting the development of the proposal (grant # 15) and interpreting some of the data for the paper. 16. Foote, M.R., R. L. Horst, E.J. Huff-Lonergan, A.H. Trenkle, F.C. Parrish, Jr., and D.C. Beitz. 2004. The use of vitamin D3 and its metabolites to improve beef tenderness. J. Anim.Sci. 82:242249. (approximate percentage contribution – 40%). This paper reports work done by Monica Foote for her Master of Science under Don Beitz and was done in collaboration with the ruminant nutrition group at Iowa State University and with Ron Horst of the USDA National Animal Disease Center. Dr. Huff Lonergan played a role in developing the ideas for the grant proposal that funded the work (grant #12) and in data collection and interpretation of the protein biochemistry and meat quality data. 17. Melody, J.L., S.M. Lonergan, L.J. Rowe, T.W. Huiatt, M.S. Mayes, and E. Huff-Lonergan. 2004. Early postmortem biochemical factors influence tenderness and water-holding capacity of three porcine muscles. J. Anim.Sci. 82: 1195-1205. (approximate percentage contribution – 50%). This paper reports work done by Jamie (Dodge) Melody for her Master of Science degree that she competed in the summer of 2002 under the direction of Elisabeth Huff Lonergan. Dr. Huff Lonergan obtained the funding for the project (grant # 14) assisted in some data collection, and guided the collection, analysis and interpretation of the data. Dr. Huff Lonergan served as the senior author on the paper. 18. Rowe, L.J., K.R. Maddock, S.M. Lonergan, and E. Huff-Lonergan. 2004. Influence of early postmortem protein oxidation on beef quality. J. Anim. Sci. 82:785-793. (approximate percentage contribution – 45%). This paper reports some of the work done by Laura Rowe for her Master of Science degree under the guidance of Elisabeth Huff-Lonergan. Laura graduated in the fall of 2002. Dr. Huff Lonergan obtained the funding through USDA/ National Research Initiative Competitive Grants Program (grant #16), assisted in data collection guided the research and writing of the paper and served as senior author on the paper. 19. Kuber, P.S., J.R. Busboom, E. Huff-Lonergan, S.K. Duckett, P.S. Mir, Z. Mir, R.G. McCormick, M.V. Dodson, C.T. Gaskins, J.D. Cronrath, D. J. Marks, and J.J. Reeves. 2004. Effects of biological type and dietary fat treatment on factors associated with tenderness: I. Measurements on beef longissimus muscle. J. Anim. Sci. 82:770-778. (approximate percentage contribution – 15%). This paper represents collaboration with Paul Kuber. Dr. Huff-Lonergan assisted in developing and interpreting the biochemical assays. She also reviewed the manuscript and made editorial changes prior to submission. 20. Rowe, L.J., K.R. Maddock, S.M. Lonergan, E. Huff-Lonergan. 2004. Oxidative environments decrease tenderization of beef steaks through inactivation of µ-calpain. J. Anim. Sci. 82:3254-3266. (approximate percentage contribution – 45%). This paper reports some of the work done by Laura Rowe for her Master of Science degree under the guidance of Elisabeth Huff-Lonergan. Laura graduated in the fall of 2002. Dr. Huff Lonergan obtained the funding through USDA/ National Research Initiative Competitive Grants Program (grant #16), assisted in data collection guided the research and writing of the paper and served as senior author on the paper. 21. Cummins, K.A., S.M. Lonergan, and E. Huff-Lonergan. 2004. Effect of dietary protein depletion and repletion on skeletal muscle calpastatin during early lactation. J. Dairy Sci. 87:1428-1431. (approximate percentage contribution – 30%). This paper reports work done in a collaborative effort between Iowa State University and Auburn University. Dr. Huff Lonergan played a role in data collection and interpretation of some of the protein biochemistry data that was fundamental to the paper. Elisabeth Huff Lonergan 8 22. Wertz, A.E., T. Knight, A. Trenkle, R. Sonon, R.L. Horst, E.J. Huff-Lonergan, and D.C. Beitz. 2004. Feeding 25-Hydroxyvitamin D3 to improve beef tenderness. J. Anim. Sci. 82:1410-1418. (approximate percentage contribution – 40%). This paper reports work done in collaboration with the ruminant nutrition group at Iowa State University and with Ron Horst of the USDA National Animal Disease Center. Dr. Huff Lonergan played a role in developing the ideas for the grant proposals that funded the work (#12 and #17) and in data collection and interpretation of the protein biochemistry and meat quality data. 23. Maddock, K. R., E. Huff-Lonergan, L. J. Rowe, and S. M. Lonergan. 2005. Effect of pH and ionic strength on µ- and m-calpain inhibition by calpastatin. J. Anim. Sci. 83:1370-1376. (approximate percentage contribution – 40%). Dr. Huff-Lonergan was the PI on the grants that funded the work (#16 and #26). She also aided in developing the assays and in editing the manuscript. 24. Huff-Lonergan, E., and S.M. Lonergan. 2005. Mechanisms of water-holding capacity of meat: The role of postmortem biochemical and structural changes. Meat Sci. 71:194-204. (approximate percentage contribution – 90%). This paper was an invited review. Dr. Huff-Lonergan was the invited author and wrote the majority of the manuscript. In the period of 2005-2009, this paper was the fourth most downloaded paper for the journal Meat Science. 25. Gardner, M.A., E. Huff-Lonergan, L.J. Rowe, C.M. Schultz-Kaster, and S.M. Lonergan. 2006. Influence of harvest processes on pork loin and ham quality. J. Anim. Sci. 84:178-184. (approximate percentage contribution – 40%). This paper reports work done by Matt Gardner for his Master of Science degree under Steven Lonergan. Matt graduated in the spring of 2003. Dr. Huff Lonergan played a role in assisting in developing the proposal that funded the work, (grant # 20), collecting and interpreting the data for the paper. 26. Bee, G., C. Biolley, G. Guex, W. Herzog, S.M. Lonergan, and E. Huff-Lonergan. 2006. Effects of available dietary carbohydrate and pre-slaughter treatment on glycolytic potential, protein degradation and quality traits of pig muscles. J. Anim. Sci. 84:191-203. (approximate percentage contribution – 15%). This paper represents collaboration with Dr. Bee. Dr. Huff-Lonergan assisted in developing and interpreting the biochemical assays. She also reviewed the manuscript and made editorial changes prior to submission. She was also involved in responding to reviewers’ comments. 27. Carlin, K. R., E. Huff-Lonergan, L. J. Rowe, and S. M. Lonergan. 2006. Effect of oxidation, pH, and ionic strength on calpastatin inhibition of µ- and m-calpain. J. Anim. Sci. 84:925-937. (approximate percentage contribution – 40%). Dr. Huff-Lonergan was the PI on the grants that funded the work (#16 and #26). She also aided in developing the assays and in editing the manuscript. 28. Zhang, W.G., E. Huff-Lonergan, and S.M. Lonergan. 2006. The role of integrin and desmin in water-holding capacity in pork. Meat Science 74:578-585. (approximate percentage contribution – 70%). Dr. Huff-Lonergan was the PI on the grants that funded the work (#21 and #22). She also aided in developing the assays and in writing and editing the manuscript as well as taking the lead in responding to reviewers’ comments. 29. Aryeetey, R., G.S. Marquis, E. Colcraft, O. Sakyi-Dawson, A. Lartey, B. Ahunu, E. Canacoo, L.M. Butler, M.B. Reddy, H.H. Jensen, and E. Huff-Lonergan. 2006. Constraints on the use of animal source foods for young children in Ghana: A participatory rapid appraisal approach. Ecology of Food and Nutrition 45:351-377. (approximate percentage contribution – 10%). Dr. Huff-Lonergan was a co-PI on the grants that funded this work (grants #25 and # 29). She was also involved in editing the manuscript and in responding to reviewers comments. Elisabeth Huff Lonergan 9 30. Custodio, M. G., W. J Powers, E. Huff-Lonergan, M.A. Faust, J. Stein. 2006. Growth, pork quality, and excretion characteristics of pigs fed Bt corn or non-transgenic corn. Can. J. Anim. Sci 86:461-469. (approximate percentage contribution – 30%). Dr. Huff-Lonergan contributed to data collection (color data) and interpretation of the data from the entire project She was on the student’s (Custodio) graduate committee. She was co-PI on the grant that funded the project (grant #27) She also contributed to the editing of the manuscript and in responding to reviewers’ comments. 31. Bee, G., A.L. Anderson, S.M. Lonergan, and E. Huff-Lonergan. 2007. Rate and extent of pH decline affect proteolysis of cytoskeletal proteins and water-holding capacity in pork. Meat Science 76:359-365. (approximate percentage contribution – 40%). Dr. Huff-Lonergan was the PI on the grants that funded the work (#21 and #22). She also aided in developing the assays and in editing the manuscript. 32. Lonergan, S. M., K. J. Stalder, T. J. Knight, E. Huff-Lonergan, R. N. Goodwin, K.J. Prusa, and D. C. Beitz. 2007. Influence of lipid content on pork sensory quality within pH classification. J. Anim. Sci. 85:1074-1079. (approximate percentage contribution – 20%). Dr. Huff-Lonergan contributed to the data interpretation and to the editing and preparation of the manuscript. 33. Huff-Lonergan, E. and S.M. Lonergan. 2007. New frontiers in understanding drip loss in pork: Recent insights on the role of postmortem muscle biochemistry. (Invited Review). (approximate percentage contribution – 90%). J. Anim. Breed. Genet. 124 (supplement 1):19-26. This paper was an invited review. Dr. Huff-Lonergan was the invited author and wrote the majority of the manuscript. 34. Carnagey, K.M., E.J. Huff-Lonergan, A. Trenkle, A.E. Wertz-Lutz, R.L. Horst, and D.C. Beitz. 2008. Use of 25-hydroxyvitamin-D2 and vitamin E to improve tenderness of beef from Longissimus dorsi of heifers. J. Anim. Sci. 86:1649-1657. (approximate percentage contribution – 40%). Dr. Huff-Lonrgan was involved in writing and obtaining the grants that funded the project (grants #30 and #32). She aided in developing, implementing and interpreting the biochemical tenderness measurement that was used in the project. She also served on the student’s graduate committee and aided in editing the manuscript for submission. 35. Patton, B.S. E. Huff-Lonergan, M.S. Honeyman, B.J.Kerr, and S.M. Lonergan. 2008. Effects of space allocation within a deep bedded finishing system on swine growth performance, fatty acid composition and pork quality. Animal: 2:471-478. (approximate percentage contribution – 20%). Dr. Huff-Lonergan contributed to data collection (color data) and interpretation of the data from the entire project. She also contributed to the editing of the manuscript. 36. Patton, B.S. E. Huff-Lonergan, M.S. Honeyman, B.J.Kerr, and S.M. Lonergan. 2008. Effects of deep bedded finishing system on market swine performance, compostion and pork quality. Animal: 2:459-470. (approximate percentage contribution – 20%). Dr. Huff-Lonergan contributed to data collection (color data) and interpretation of the data from the entire project. She also contributed to the editing of the manuscript. 37. Barbut, S., A.A. Sosnicki, S.M. Lonergan, T. Knapp, D.C. Ciobanu, L.J. Gatcliffe, E. HuffLonergan, E.W. Wilson. 2008. Progress in reducing the pale, soft and exudative (PSE) problem in pork and poultry meat. Meat Science 79:46-63. (approximate percentage contribution – 20%). Research that was conducted by Dr. Huff-Lonergan and was from grants that she was PI or Co-PI on was used extensively in this paper (grants # 11, #14, #15, #16, #21, #22, #35). She also contributed figures and was involved in editing the manuscript. Elisabeth Huff Lonergan 10 38. Carnagey, K.M., E.J. Huff-Lonergan, S.M. Lonergan, A. Trenkle, R.L. Horst, D.C. Beitz. 2008. Use of 25-hydroxyvitamin D3 and dietary calcium to improve tenderness of beef from the round of beef cow. J. Anim. Sci. 86:1637-1648. (approximate percentage contribution – 35%). Dr. HuffLonrgan was involved in writing and obtaining the grants that funded the project (grants #30 and #32). She aided in developing, implementing and interpreting the biochemical tenderness measurement that was used in the project. She also served on the student’s graduate committee and aided in editing the manuscript for submission. 39. Lametsch, R., S. Lonergan, E. Huff-Lonergan. 2008. Disulfide bond within µ-calpain active site inhibits activity and autolysis. Biochemica et Biohphysica Acta – Proteins and Proteomics. 1784:1215-1221. (approximate percentage contribution – 35%) Dr. Huff-Lonergan was responsible for obtaining the funding, developing assays and assisted in writing the manuscript 40. Cupp, A.S., J. Matthews, E. Huff-Lonergan, D.M. Spurlock, and D. McLean. 2008 Cell biology symposium: The role of microRNA in cell function. J. Anim. Sci 87: E19-E20. Dr. Huff-Lonergan was a coordinator of the symposium, aided in writing the overview and was instrumental in obtaining funding for the symposium. 41. Kim, Y-H., Lonergan, S.M., Sebranek, J.G, and Huff-Lonergan, E. 2010. High-oxygen modified atmosphere packaging system induces lipid and myoglobin oxidation and protein polymerization. Meat Science 85:759-767. Dr. Huff-Lonergan assisted in the collection and interpretation of the data and consulted on manuscript preparation. 42. Kim, Y-H., Lonergan, S.M. and Huff-Lonergan, E., 2010. Protein denaturing conditions in beef deep semimembranosus muscle results in limited µ-calpain activation and protein degradation. Meat Science. 86:883-887. Dr. Huff-Lonergan assisted in the collection and interpretation of the data and consulted on manuscript preparation. 43. Huff-Lonergan, E., Zhang, W., Lonergan, S.M. 2010. Biochemistry of postmortem muscle – Lessons on mechanisms of meat tenderization. Meat Science 86:184-195. Dr. Huff-Lonergan assisted in the collection and interpretation of the data and wrote 80% of the manuscript preparation. 44. Kim, Y-H., Huff-Lonergan, E., Sebranek, J.G., and Lonergan, S.M. 2010. Effects of lactate/phosphate injection enhancement on oxygen stability and protein degradation in early postmortem beef cuts packaged in high oxygen modified atmosphere. Meat Science. 86:852-858. Dr. Huff-Lonergan assisted in the collection and interpretation of the data and consulted on manuscript preparation. 45. Fan, B., Lkhagvadorj, S., Cai, W., Young, J., Smith, R.M., Dekkers, J.C.M., Huff-Lonergan, E., Lonergan, S.M., Rothschild, M.F. 2010. Identification of genetic markers associated with residual feed intake and meat quality traits in the pig. Meat Science. 84:645-650. Dr. Huff-Lonergan assisted in the collection and interpretation of the data and consulted on manuscript preparation. 46. Smith, R. M., N. K. Gabler, J.M. Young, W. Cai, N. J. Boddicker, M. J. Anderson, E. HuffLonergan, J.C.M. Dekkers, S.M. Lonergan. 2010. Effects of selection for decreased residual feed intake on composition and quality of fresh pork. J. Animal Science. 89:192-200. Dr. Huff-Lonergan assisted in the collection and interpretation of the data and consulted on manuscript preparation. 47. Anderson, M.J., S.M. Lonergan, and E. Huff-Lonergan. 2012 (First published on line in August 2011). Myosin Light Chain 1 release from myofibrillar fraction during postmortem aging is a Elisabeth Huff Lonergan 11 potential indicator of proteolysis and tenderness of beef. Meat Science. 90:345-351. Dr. HuffLonrgan was involved in writing and obtaining the grants that funded the project. She aided in developing, implementing and interpreting the biochemical tenderness measurement that was used in the project. She is the student’s major professor and aided in editing the manuscript for submission. 48. Kim, Y.H. E. Huff-Lonergan, and S.M. Lonergan. 2012. Effect of calcium lactate on m-calpain activity and protein degradation under oxidizing conditions. Food Chemistry. 131:73-78 (Published on-line August, 2011). Dr. Huff-Lonergan assisted in the collection and interpretation of the data and consulted on manuscript preparation. 49. Anderson, M.J., Lonergan, S.M., Fedler, C., Prusa, K., Binning, J., and Huff-Lonergan, E. 2012. Profile of biochemical traits influencing tenderness of muscles from the beef round. Meat Sci 91:247-254 Dr. Huff-Lonrgan was involved in writing and obtaining the grants that funded the project. She aided in developing, implementing and interpreting the biochemical tenderness measurement that was used in the project. She is the student’s major professor and aided in editing the manuscript for submission. 50. Lonergan, S.M. and E. Huff-Lonergan. 2012 Application of science, technology, and art in producing meat: A recipe for success. Animal Frontiers. 2:5. doi:10.2527/af.2012-0055 Co wrote the article. 51. Grubbs, J, Fritchen, A., Huff-Lonergan, E., Gabler, N., Lonergan, S.M. 2013. Selection for residual feed intake alters the mitochondria protein profile in pigs. J. Proteomics 80:334-345. Dr. Huff-Lonergan assisted in the collection and interpretation of the data and consulted on manuscript preparation. 52. Grubbs, J, Fritchen, A., Huff-Lonergan, E., Gabler, N., Lonergan, S.M. 2013. Divergent genetic selection for residual feed intake impacts mitochondrial reactive oxygen species production. J. Anim.Sci 91:2133-2140. Dr. Huff-Lonergan assisted in the collection and interpretation of the data and consulted on manuscript preparation. 53. Kim, Y.H., Lonergan, S.M., Grubbs, J.H., Cruzen, S.M., Fritchen, A.N., Marino, A. 2013. Effect of low voltage electrical stimulation on protein and quality changes in bovine muscles during postmortem aging. Meat Science 94:289-296. Dr. Huff-Lonergan assisted in the collection and interpretation of the data and consulted on manuscript preparation. 54. Cruzen, S.M., A.J. Harris, K. Hollinger, R.M. Punt, J.K. Grubbs, J.T. Selsby, J.C.M. Dekkers, N.K. Gabler, S.M. Lonergan, E. Huff-Lonergan. 2013. Evidence of decreased muscle protein turnover in gilts selected for low residual feed intake. Journal of Animal Science 91:2133-2140. Dr. HuffLonergan was involved in writing and obtaining the grants that funded the project. She aided in developing, implementing and interpreting the biochemical measurements that were used in the project. She is the student’s major professor and aided in editing the manuscript for submission. 55. Ros-Freixedes, R., L.J. Sadler, S.K. Onteru, R.M. Smith, J.M. Young, A.K. Johnson, S.M. Lonergan, E. Huff-Lonergan, J.C.M. Dekkers, and M.F. Rothschild. 2014. Relationship between gilt behavior and meat quality using principal component analysis. Meat Science 96:264-269. Dr. Huff-Lonergan assisted in the collection and interpretation of the data and consulted on manuscript preparation. 56. Cruzen, S. M., P.V.R. Paulino, S M. Lonergan, E. Huff-Lonergan. 2014. Postmortem proteolysis in three muscles from growing and mature beef cattle. Meat Science 96:854-861. Dr. Huff-Lonergan was involved in writing and obtaining the grants that funded the project. She aided in developing, implementing and Elisabeth Huff Lonergan 12 interpreting the biochemical tenderness measurement that was used in the project. She is the student’s major professor and aided in editing the manuscript for submission. 57. Mohrhauser, D.A. S.M. Lonergan, E. Huff-Lonergan, K.R. Underwood, and A.D. Weaver. 2014. Calpain-1 activity in bovine muscle is primarily influenced by temperature, not pH decline. J. Anim. Sci. Published ahead of print, Jan 2014. Dr. Huff-Lonergan assisted in the interpretation of the data and consulted on manuscript preparation. 58. Anderson, M., Lonergan, S.M., Huff-Lonergan. 2014. Differences in phosphorylation of phosphoglucomutase 1 in beef steaks from the longissimus dorsi with high or low star probe values. Meat Science 96:379-384. Dr. Huff-Lonergan was involved in writing and obtaining the grants that funded the project. She aided in developing, implementing and interpreting the biochemical tenderness measurement that was used in the project. She is the student’s major professor and aided in editing the manuscript for submission. 59. Ros-Freixedes, R., Sadler, L., Onteru, S.K., Smith, R., Young, J.M., Johnson, A.K., Lonergan, S.M. Huff-Lonergan, E., Rothschild, M.F. 2014. Relationship between gilt behavior and meat quality using principal component analysis. Meat Science 96:264-269. Dr. Huff-Lonergan assisted in the interpretation of the data and consulted on manuscript preparation. 60. Grubbs, J., Huff-Lonergan, E., Gabler, N., Dekkers, J.C.M., Lonergan, S.M. 2014. Liver and skeletal muscle mitochondria proteomes are altered in pigs divergently selected for residual feed intake. Journal of Animal Science. 92:1995-2007. Dr. Huff-Lonergan assisted in the interpretation of the data and consulted on manuscript preparation. 61. Marquis, G.S., Colecraft, E.K., Owuraku, S-D., Lartey, A., Ahunu, B.K., Birks, K.A., Butler, L.M., Reddy, M.B., Jensen, H.H., Huff-Lonergan, E. 2015. An integrated microcredit, entrepreneurial training and nutrition education intervention is associated with better weight – but not heightrelated indicators among pre-school-aged children in rural Ghana. The Journal of Nutrition. 145:335-343. Dr. Huff-Lonergan assisted in the collection and interpretation of the data and consulted on manuscript preparation. 62. Arkfeld, E., Young, J., Johnson, R., Fedler, C., Prusa, K., Patience, J., Dekkers, J., Lonergan, S., Gabler, N., Huff-Lonergan, E., 2015. Compositon and quality characteristics of carcasses from pigs divergently selected for residual feed intake on high or low energy diets. Journal of Animal Science. (Accepted with revisions). Dr. Huff-Lonergan was involved in writing and obtaining the grants that funded the project. She aided in developing, implementing and interpreting quality and biochemical traits that were evaluated in the project. She was the student’s major professor and aided the student in editing the manuscript for submission 63. Grubbs, J., Tuggle, C., Dekkers, J., Boddicker, N., Nguyen, Y., Huff-Lonergan, E., Nettleton, D., Lonergan, S., 2015. Investigation of the efficacy of albumin removal procdures on porcine serum proteome profile. Journal of Animal Science. (Accepted with revisions). Dr. Huff-Lonergan assisted in the collection and interpretation of the data and consulted on manuscript preparation. Elisabeth Huff Lonergan 13 Peer-Reviewed, Invited Book Chapters 1. Lonergan, E.H., D.D. Beekman, and F.C. Parrish, Jr. 1994. Protein separation and analysis of certain skeletal muscle proteins: Principles and techniques. In: N.S. Hettiarachy and G.R. Ziegler (Ed.) Protein Functionality in Food, Marcel Dekker, Inc. New York. pp. 79-119. 2. Huff-Lonergan, E.J., and S.M. Lonergan. 1999. Postmortem mechanisms of meat tenderization: The roles of the structural proteins and the calpain system. In: Quality attributes of muscle foods. Y.L. Xiong, C-T. Ho, and F. Shahidi (eds.) Kluwer Academic/Plenum Publishers, New York. pp 229-251. 3. Huff-Lonergan, E., S. M. Lonergan, and D. Beermann. 2003. Growth and meat quality. In: Biology of Growth of Domestic Animals. C. Scanes (ed.) Iowa State University Press/Blackwell, Ames, Iowa. pp 220-232. 4. Hopkins, D.L., and E. Huff-Lonergan. 2004. Tenderising mechanisms: Chemical and enzymatic. In: Encyclopedia of Meat Sciences. Elsevier Science. pp. 1363-1369. 5. Huff-Lonergan, E. and S.M. Lonergan. 2009. Water-holding Capacity. In: Improving the Sensory and Nutritional Quality of Fresh Meat. J. Kerry and D. Ledward (eds). Woodhead Publishing Co., London, UK. 6. Ciobanu, D. C., S.M. Lonergan, and E. Huff-Lonergan. 2011. Genetics of meat quality and carcass traits. In: Genetics of the Pig. Second Edition. A. Ruvinsky and M.F. Rothschild (eds). CABI International. Oxfordshire, UK. 7. E. Huff-Lonergan. 2014 Tenderising mechanisms: Enzymatic. In: Encyclopedia of Meat Sciences. Elsevier Science. In press. U.S. Patents 1. Use of Vitamin D, its Metabolites and Analogs to improve tenderness of meat and meat products. Patent No. 6,042,588. Issue Date; 3/29/2000 Donald Beitz, Ronald Horst, Jayden Montgomery, Frederick Parrish, Allen Trenkle, Elisabeth Huff-Lonergan. Published Conference Proceedings 1. Huff, E.J., F.C. Parrish, Jr. and R.M. Robson. 1992. Changes in myofibrillar/cytoskeletal proteins of bovine longissimus muscle differing in tenderness. Proceedings 38th International Congress of Meat Science and Technology. pp. 371-374. 2. Huff-Lonergan, E., F.C. Parrish, Jr., D.G. Olson, and R.M. Robson. 1995. Degradation of muscle structural proteins by µ-calpain under conditions simulating postmortem pH, temperature and ionic strength. Proceedings 41st International Congress of Meat Science and Technology. pp. 545-546. Elisabeth Huff Lonergan 14 3. Huff-Lonergan, E. 1997. Methods to monitor/quantify postmortem changes in the muscle proteins. Proc. Recip. Meat Conf. 50:141-143. 4. Robson, R.M., E. Huff-Lonergan, F.C. Parrish, Jr., C.-Y. Ho, M.H. Stromer, T.W. Huiatt, R.M. Bellin, and S.W. Sernett. 1997. Postmortem changes in the myofibrillar and other cytoskeletal proteins in muscle. Proc. Recip. Meat Conf. 50:43-52. 5. Huff-Lonergan, E., and J. K. Page. 2000. Chilling effects on quality. Pork Quality and Safety Summit Proceedings. National Pork Producers Council. pp. 251-260. 6. Huff-Lonergan, E., S.M. Lonergan, and L. Vaske. 2000. pH relationships to quality attributes: Tenderness. Proceedings of the workshop "pH: An objective predictor of pork quality". American Meat Science Association and the National Pork Producers Council (http://www.meatscience.org/porkph/). 7. Huff-Lonergan, E. S.M. Lonergan, J. Dodge, and L. Rowe. 2002. Biochemical aspects of meat tenderness. Proceedings of the workshop on pork measures. American Meat Science Association and the National Pork Board. Published on CD-ROM. 8. Huff-Lonergan, E., J. Melody, R. Klont, and A. Sosnicki. 2003. Pork quality: Current and future needs of industry and academia. Proc. Recip. Meat Conf. 56:23-29. 9. Lonergan, S. M., E. Huff-Lonergan. 2004. Influence of protein nutrition on calpastatin in skeletal muscle. FASEB Summer Research Conference; “ Biology of the Calpains in Health and Disease”. June 12-17, 2004. Tucson, AZ. 10. Maddock, K. R., E. Huff-Lonergan, L. J. Rowe, and S. M. Lonergan. 2004. Activity of µ-and mcalpain inhibition by calpastatin is affected by oxidation, pH, and ionic strength. FASEB Summer Research Conference; “ Biology of the Calpains in Health and Disease”. June 12-17, 2004. Tucson, AZ. 11. Huff-Lonergan, E. and S. M. Lonergan. 2004. Oxidation of calpain limits proteolysis and meat tenderization. FASEB Summer Research Conference; “ Biology of the Calpains in Health and Disease”. June 12-17, 2004. Tucson, AZ. 12. Weaver, A., S.M. Lonergan. E. Huff-Lonergan. 2007. Techniques in gel elecrophoresis and western blotting. Proc. Recip. Meat Conf. (on-line publication) http://www.meatscience.org/Pubs/rmcarchv/2007/index.html 13. Zhang, W-G., S.M. Lonergan, E. Huff-Lonergan. 2007. S-nitrosoglutathione influences the activiy of µ and m-calpain. International Congress of Meat Science and Technology. August 4-10, 2007. Beijing, China. 14. Lametsch, R., E. Huff-Lonergan, S.M Lonergan. 2007. Disulfide bond within µ-calpain active site inhibits activity and autolysis. FASEB Summer Research Conference. “Biology of Calpain in Health and Disease”. Snowmass Village, Colorado. July 19, 2007 15. Huff-Lonergan, E., S.M. Lonergan, K. Maddock Carlin. 2007. New insights on calpain regulation in postmortem skeletal muscle. FASEB Summer Research Conference. “Biology of Calpain in Health and Disease”. Snowmass Village, Colorado. July 19, 2007 16. Huff-Lonergan, E., S.M. Lonergan. 2008. Relationship of postmortem changes in myofibrillar protein to meat quality. Part of an international symposium titled “Postmortem Changes in Elisabeth Huff Lonergan 15 17. 18. 19. 20. 21. 22. Myofibrillar Protein and the Associated Contribution to Meat Quality.” American Society of Animal Science Annual Meeting. July, 2008. Huff-Lonergan, E. 2008. Interaction between the muscle contraction system and proteolytic tenderization in beef. 54th International Congress of Meat Science and Technology (ICoMST). August 14, 2008. Huff-Lonergan, E., M.J. Anderson, and S.M. Lonergan. 2011. Proteomic applications for meat tenderness. American Meat Science Association. 64th RMC Proceedings. http:/www.meatscience.org/ Weaver, A., S.M. Lonergan. E. Huff-Lonergan. 2007. Techniques in gel electrophoresis and western blotting. Proc. Recip. Meat Conf. (on-line publication) http://www.meatscience.org/Pubs/rmcarchv/2007/index.html Huff-Lonergan, E. M. J. Anderson, and S. M. Lonergan. 2011. Proteomic applications for meat tenderness. American Meat Science Association. 64th RMC Proceedings. http://www.meatscience.org/Page.aspx?ID=7497. Huff-Lonergan, E., S.M. Lonergan, S. Cruzen. 2013. Biochemisty of muscle: Relevance of postmortem changes to aging. FASEB Summer Research Conference Proceedings. “Biology of Calpain in Health and Disease”. Saxtons River Vermont. July 25, 2013. Huff-Lonergan, E., S.M. Lonergan, S.M. Cruzen, K. Grubbs, J. Dekkers, N. Gabler, J. Selsby, L. Baumgard. 2014. Use of proteomics for livestock improvement. American Society of Animal Science Annual Meeting. Kansas City, Mo. July 23, 2014. Elisabeth Huff Lonergan 16 Published Abstracts 1. Huff, E.J., and F.C. Parrish, Jr. 1991. The effects of animal age, sex and postmortem aging time on selected palatability attributes of beef steaks. J. Anim. Sci.(Suppl. 1) 69:97. 2. Huff, E.J., F.C. Parrish, Jr., and R.M. Robson. 1991. The effects of sex, animal age and postmortem aging time on selected characteristics of bovine longissimus muscle. Proc. Recip. Meat Conf. 44:206. 3. Huff, E.J., D.D. Beekman, F.C. Parrish, Jr., and R.M. Robson. 1993. Postmortem aging and injection treatments to enhance beef tenderness. Proc. Recip. Meat Conf. 46:67. 4. Kahtri, Y., E.J. Huff, F.C. Parrish, Jr., and R.M. Robson. 1993. Effect of postmortem pH on the physical, chemical and sensory characteristics of bovine longissimus thoracis muscle. J. Anim. Sci.(Suppl. 1) 71:51. 5. Thompson, V.F., R.G. Taylor, D.E. Goll, E.J. Huff, and R.M. Robson. 1993. Effect of µ- and mcalpain on bovine skeletal muscle myofibrils and IZI brushes. Mol. Biol. Cell. 4:386a. 6. Beekman, D.D., E.J. Huff-Lonergan, F.C. Parrish, Jr., R.M. Robson, and T. Mitsuhashi. 1994. The effect of sodium chloride, tripolyphosphate, and calcium injections on the tenderness and water holding capacity of beef. Proc. Recip. Meat Conf. 47:87. 7. Huff-Lonergan, E., T. Mitsuhashi, F.C. Parrish, Jr., R.M. Robson, and D.D. Beekman. 1995. Protein degradation in postmortem aged beef longissimus and calpain digested myofibrils. J. Anim. Sci.(Suppl. 1) 73:62. 8. Huff-Lonergan, E., D.L. Kuhlers, S.M. Lonergan, W.B. Mikel, S.B. Jungst, S. Dale, and V.D. Reed. 1997. Pork quality decline in response to selection for lean growth efficiency in the absence of the halothane gene. J. Anim. Sci.(Suppl. 1) 75:176. 9. Huff-Lonergan, E., S.M. Lonergan, L.A. Kriese, and B.R. Wiegand. 1997. Postmortem protein degradation and calpastatin activity in top loin steaks from Brangus cattle. J. Anim. Sci.(Suppl. 1) 75:176. 10. Huff-Lonergan, E., S. Harris, D. Kuhlers, S. Jungst, S. Lonergan and W.B. Mikel. 1997. Selection for lean growth efficiency: Effects on selected fresh and processed pork quality attributes. Proc. Recip. Meat Conf. Volume 50:160. 11. Liu, E., R.L. Kelly, E. Huff-Lonergan, C.H. Rahe, and D.R. Mulvaney. 1997. Effects of IGF’s on Rb protein in primary skeletal muscle culture. J. Anim. Sci. (Suppl. 1) 75:166. 12. Lonergan, S.M., E. Huff-Lonergan, and D.M. Payne. 1997. Identification and purification of a high molecular weight bovine skeletal muscle calpastatin. Proc. Recip. Meat Conf. 50:169. 13. Lonergan, S.M., E. Huff-Lonergan, and D.M. Payne. 1997. Purification and partial characterization of a high molecular weight bovine skeletal muscle calpastatin. FASEB J. 11:337. Elisabeth Huff Lonergan 17 14. Lonergan, S.M., E. Huff-Lonergan, B.R. Wiegand, and L.A. Kriese. 1997. The relationship of calpastatin activity to Warner-Bratzler shear force during aging of top loin steaks from Brangus cattle. J. Anim. Sci. (Suppl. 1) 75:12. 15. Montgomery, J.L., F.C. Parrish, Jr., D.C. Beitz, R.L. Horst, E.J. Huff-Lonergan, and A.H. Trenkle. 1997. The use of vitamin D3 to improve beef tenderness. Proc. Recip. Meat Conf. 50:168. 16. Huff-Lonergan, E., D.L. Kuhlers, S.M. Lonergan, and S.B. Jungst. 1998. Characterization of pork quality in response to five generations of selection for lean growth efficiency. J. Anim. Sci.(Suppl. 1) 76:151. 17. Huff-Lonergan, E., S.E. Harris, D.L. Kuhlers, S.M. Lonergan, and S.B. Jungst. 1998. Processing characteristics of cured products from Duroc pigs selected for lean growth efficiency. J. Anim. Sci.(Suppl. 1) 76:152. 18. Kuhlers, D.L., S.B. Jungst, E. Huff-Lonergan, S.M. Lonergan, B.L. Anderson, and B.E. Gamble. 1998. Carcass characteristics of Landrace pigs from a line selected for loin eye area using real-time ultrasound technology. J. Anim. Sci.(Suppl. 1) 76:52. 19. Lonergan, S.M., E. Huff-Lonergan, and L.A. Kriese. 1998. Characterization of factors associated with the postmortem tenderization in top loin steaks from Brangus cattle. J. Anim. Sci.(Suppl. 1) 76:154. 20. Lonergan, S.M., E. Huff-Lonergan, L.A. Kriese-Anderson, and R.L. Kelley. 1998. Examination of sire breed differences for postmortem protein degradation, calpastatin activity, and WarnerBratzler shear force in top loin steaks from cattle finished in the southeastern United States. J. Anim. Sci.(Suppl. 1) 76:155. 21. Huff-Lonergan, E., S.M. Lonergan. 1998. Postmortem mechanisms of meat tenderization: The roles of the structural proteins and the calpain system. Abstracts of papers of the American Chemical Society. 215: U13-U13, Part 1. 22. Chiba, L.I., D.L. Kuhlers, L.T, Frobish, S.B. Jungst, E.J. Huff-Lonergan, S.M. Lonergan, and B.L. Anderson. 1999. Dietary restrictions and growth performance and carcass traits of pigs selected for lean growth efficiency. J. Anim. Sci.(Suppl 1) 77:192. 23. Harris, S.E., E. Huff-Lonergan, and S.M. Lonergan. 1999. Color stability and tenderness properties of beef from vitamin E fed cattle. J. Anim. Sci.(Suppl 1) 77: 46. 24. Huff-Lonergan, E.J. 1999. Possible role and regulation of the calpain/calpastatin system in meat quality. J. Anim. Sci.(Suppl. 1) 77:120. 25. Huff-Lonergan, E., S.M. Lonergan, E. Helman, S.Gaasch, K.A. Cummins, M.G. Hayek, and G.M. Davenport. 1999. Impact of age and diet on calpastatin and troponin-T in young and old dogs. FASEB J. 13:A938. 26. Huff-Lonergan, E., S.M. Lonergan, S.B. Jungst, and D.L. Kuhlers. 1999. Selection for lean growth efficiency in Duroc pigs: Impact on pork quality. Muscle Biochemistry Invited Poster Session. Proc. Recip. Meat Conf. 52:127. Elisabeth Huff Lonergan 18 27. Lonergan, S.M., E. Huff-Lonergan, L.I. Chiba, L.T. Frobish, S.B. Jungst, and D.L. Kuhlers. 1999. Influence of amino acid content of grower and finisher diets on quality characteristics of pork from Duroc pigs selected for lean growth efficiency. J. Anim. Sci.(Suppl 1) 77:45. 28. Tyler, P.J., S. Lonergan, E. Huff-Lonergan, and K.A. Cummins. 1999. Effect of reduced dietary protein intake on calpastatin content of skeletal muscle in lactating dairy cows. Dairy Sci.(Suppl. 1) 82:68. 29. Huff-Lonergan, E., T.J. Baas, M. Malek, J. Dekkers, K Prusa, and M.F. Rothschild. 2000. Correlations among selected pork quality traits in a Berkshire by Yorkshire F2 population. J. Anim. Sci.(Suppl. 1) 78:160. 30. Malek, M., J.C.M. Dekkers, H.K. Lee, T.J. Baas, K. Prusa, E. Huff-Lonergan, and M.F. Rothschild. 2000. A molecular genome scan analysis to identify chromosomal regions influencing meat quality in the pig. J. Dairy Sci.(Suppl. 1) 83:77. 31. Malek, M., J.C.M. Dekkers, H.K. Lee, T.J. Baas, K. Prusa, E. Huff-Lonergan, and M.F. Rothschild. 2000. A quantitative trait loci analysis for growth and meat quality traits in the pig. In: J.A.M. Van Arendonk (Editor), Book of abstracts of the 51st Annual Meeting of the European Association for Animal Production. p. 89. Wageningen Pers, Wageningen, The Netherlands. 32. Helman, E.E., E. Huff-Lonergan, S.M. Lonergan and G.M. Davenport. 2001. The effect of dietary treatments on the endogenous calpain/calpastatin proteolytic system in canine skeletal muscle. J. Anim. Sci.(Suppl. 1) 79:430. 33. Rowe, L.J., S.M. Lonergan, M.F. Rothschild, and E. Huff-Lonergan. 2001. Desmin degradation influences water-holding capacity and tenderness of fresh pork. J. Anim. Sci.(Suppl. 1), 79:20. 34. Rowe, L.J., E. Huff-Lonergan, G.H. Rouse, D.E. Wilson, and S.M. Lonergan. 2001. Relationship between a measure of troponin-T degradation and beef tenderness. J. Anim. Sci.(Suppl. 1) 79:443. 35. Rowe, L. J., S.M. Lonergan, M.F. Rothschild, and E. Huff-Lonergan. 2001. Relationship between porcine longissimus dorsi pH decline and µ-calpain activity/autolysis and protein degradation. J. Anim. Sci.(Suppl. 1) 79:442. 36. Ciobanu, D.C., R.E. Klont, S.M. Lonergan, J.W.M. Bastiaansen, J.R. Woolard, M. Malek, E.J. Huff Lonergan, G.S. Plastow, A. Sosnicki, and M.F. Rothschild. 2002. Associations of new calpastatin alleles with meat quality traits in pigs. Proc. 48th International Congress of Meat Science and Technology. Vol. II: pp. 614-615. 37. Ciobanu, D. C., S. M. Lonergan, J. Bastiaansen, J. R. Woolard, M. Malek, E. J. Huff-Lonergan, G. S. Plastow, and M. F. Rothschild. 2002. Discovery of new genetic markers affecting meat quality. Plant, Animal & Microbe Genomes X, January 12-16, 2002, San Diego. 38. Ciobanu, D.C., S.M. Lonergan, J. Bastiaansen, J.R. Woollard, M. Malek, E. Huff-Lonergan, G.S. Plastow, and M.F. Rothschild. 2002. Evidence for new alleles in calpastatin associated with meat quality traits in pigs. World Congress of Genetics Applied in Livestock Production. 31:355-358. 39. Dodge, J., E. Huff-Lonergan, S. . Lonergan, L.J. Rowe. 2002. µ-Calpain autolysis and calpastatin activity influences drip loss and tenderness of three porcine muscles. J. Anim. Sci.(Suppl. 2) 80:52. Elisabeth Huff Lonergan 19 40. Gardner, M.A., S.M. Lonergan, E. Huff-Lonergan, L.J. Rowe, and C.M. Schultz-Kaster. 2002. Influence of harvest processes on pork loin and ham quality. J. Anim. Sci.(Suppl. 2) 80:52. 41. Lonergan, S.M., E. Huff-Lonergan, and M. F. Rothschild. 2002. Characterization of muscle glycogen storage and utilization: Influence on pork quality. J. Anim. Sci.(Suppl. 2) 80:54. 42. Asmus, A., E. P. Berg, J. L. Melody, S. M. Lonergan, and E. Huff-Lonergan. 2003. Degradation of calcium regulating and intermediate filament proteins is related to fresh pork quality. J. Anim. Sci.(Suppl. 1) 81:74. 43. Maddock, K.R., E. Huff-Lonergan, L.J. Rowe, and S.M. Lonergan. 2003. Effect of pH and ionic strength on calpastatin inhibition of µ- and m-calpain. J. Anim. Sci.(Suppl. 1) 81:74. 44. Maddock, K.R., L.J. Rowe, E. Huff-Lonergan, and S.M. Lonergan. 2003. Effects of oxidation on inactivation of calpastatin in beef. J. Anim. Sci.(Suppl. 1) 81:74. 45. Rowe, L.J., K.R. Maddock, A. Trenkle, S.M. Lonergan, and E. Huff-Lonergan. 2003. Effects of oxidation on beef tenderness and calpain activity. J. Anim. Sci.(Suppl. 1) 81:74. 46. Rowe, L.J., K. Maddock, S.M. Lonergan, and E. Huff-Lonergan. 2003. Influence of early postmortem protein oxidation on beef quality. J. Anim. Sci.(Suppl. 1) 81:73. 47. Melody, J. L., L. J. Rowe, T. W. Huiatt, S. M. Lonergan, and E-Huff-Lonergan. 2003. Relationship between µ-calpain autolysis and immunolocalization in pork myofibrils. Proc. Recip. Meat Conf. 48. Mendez, G. Ch., E. Huff-Lonergan, J. O. Matthews, C. M. Schultz-Kaster, and S. M. Lonergan. 2004. The Effect of duration of carcass scalding on pork quality. J. Anim. Sci. 82 (Suppl. 2):56. 49. Bee, G., S. M. Lonergan, E. Huff-Lonergan. 2004. Early postmortem pH influences proteolysis of cytoskeletal proteins during aging in porcine longissimus muscle. J. Anim. Sci. 82 (Suppl.1):13. 50. Maddock, K. R., E. Huff-Lonergan, L. J. Rowe, and S. M. Lonergan. 2004. Oxidation, ionic strength, and pH affect porcine skeletal muscle calpain and calpastatin activity. J. Anim. Sci. 82 (Suppl.1):14. 51. Bee, G., S. M. Lonergan, E. Huff-Lonergan. 2004. Early postmortem pH influences proteolysis of cytoskeletal proteins during aging in porcine longissimus muscle. J. Anim. Sci. 82 (Suppl.1):13. 52. Ciobanu, D. C., S. M. Lonergan, J. W. M. Bastiaansen, A. Mileham, B. Miculinich, C. SchultzKaster, A. A. Sosnicki, G. S. Plastow, and M. F. Rothschild. 2004. Association of new calpastatin alleles with meat quality traits of commercial pigs. Proceedings, International Congress of Meat Science and Technology, Helsinki, Finland. August 8-13. p 19. 53. Custodio, M.G., W.J. Powers, E. Huff-Lonergan, M.A. Faust, and J. Stein. 2004 Growth and carcass characteristics of pigs fed biotechnologically derived and non-biotechnologically derived corn and harvested at different weights. J. Anim. Sci 82(Suppl 1):456 Elisabeth Huff Lonergan 20 54. Carnagey, K.M., S.M. Lonergan, E.J. Huff-Lonergan, R.L. Horst, A.H. Trenkle and D.C. Beitz. 2005. Effect of 25-hydroxyvitamin D3 and dietary calcium on calpastatin activity in beef muscles. FASEB J. 19 (5): A991-A991 Part 2 Suppl. S 55. Aryeetey, R. G.S. Marquis, E.K. Colecraft, M.B. Reddy, L.M. Butler, H.H. Jensen, E. HuffLonergan, O. Sakyi-Dawson; B.K. Ahunu; E.A. Canacoo; A. Lartey. 2005. Identifying constraints in using animal source foods in children's diets in Ghana FASEB J. 19 (4) A430 Part 1 Suppl. 56. Carnagey, K.M., A. E. Wertz, T.J. Knight, R.L. Horst, E.J. Huff-Lonergan, A.H. Trenkle, and D. C. Beitz. 2005. Effects of orally administered 25-hydroxyvitamin-D3 and vitamin E on calcium and vitamin D metabolites in plasma and on vitamin D metabolite in beef longissimus dorsi muscle. J. Anim. Sci.83(Suppl. 1): 16. 57. Lonergan, S. M. K. J. Stalder, T. J. Knight, E. Huff-Lonergan, R. N. Goodwin, K.J. Prusa, and D. C. Beitz. 2006. Classification of pork loin based on intramuscular lipid content to predict sensory and texture of fresh pork. J. Anim. Sci. 84 (Suppl.2): 58-59. 58. Patton, B. S., E. Huff-Lonergan, M.S. Honeyman, B.J. Kerr, J.D. Crouse, and S.M. Lonergan. 2005. Deep bedded finishing of pigs: Effects on swine performance, pork quality and adipose tissue composition. Proceedings, International Congress of Meat Science and Technology, Baltimore, Maryland. August 6-13. 59. Patton, B. S., E. Huff-Lonergan, M.S. Honeyman, B.J. Kerr, J.D. Crouse, and S.M. Lonergan. 2005. The effect of space allocation on swine performance, pork quality and adipose tissue composition. Proceedings, International Congress of Meat Science and Technology, Baltimore, Maryland. August 6-13. 60. Maddock, K. R., E. Huff-Lonergan, and S. M. Lonergan. 2005. The effect of oxidation on the interaction of calpastatin with µ-calpain. Proceedings, International Congress of Meat Science and Technology, Baltimore, Maryland. August 6-13. 61. Gardner, M., E. Huff-Lonergan, and S. M. Lonergan. 2005. Prediction of fresh pork quality using indicators of protein degradation and calpain activation. Proceedings, International Congress of Meat Science and Technology, Baltimore, Maryland. August 6-13. 62. Carnagey, K., E. Huff-Lonergan, A. H. Trenkle, R. L. Horst, S. M. Lonergan, D. C. Beitz. 2006. Effect of 25-hydroxyvitamin D3 and dietary calcium on calpastatin activity in beef muscles. FASEB J. 20: A171. 63. Huff-Lonergan, E., and S. M. Lonergan. 2006. Crossing the line between muscle and meat: The influence of muscle cell physiology on meat quality. Midwest ASAS/ADSA meeting March 20, 2006. Des Moines IA. J. Anim. Sci. 84 (Suppl.2): 72. 64. Guimaraes, S. E. F., M. F. Rothschild, E. Huff-Lonergan, A. A. Sosnicki, S.B. Jungst, M.Yu, and S. M. Lonergan. 2006. Interaction of MC4R and PRKAG3 genotypes with genetic potential for growth on meat quality traits. J. Anim. Sci. 84 (Suppl.1): 113-114. Elisabeth Huff Lonergan 21 65. Wagner, C. E., E. Huff-Lonergan, M. F. Rothschild, A. A. Sosnicki, S. B. Jungst, K. J. Prusa, and S. M. Lonergan. 2006. Selection for improvement in pig growth rate does not alter fresh pork quality. J. Anim. Sci. 84 (Suppl.1): 113. 66. Zhang. W.G., S. M. Lonergan and E. Huff-Lonergan. 2006. The role of integrin and desmin in water-holding capacity in pork. J. Anim. Sci. 84 (Suppl.1): 114. 67. Steadham, E.M., K.R. Maddock Carlin, E. Huff-Lonergan, and S.M. Lonergan. 2006. Response of - and m-calpains in the presence of calpastatin after hydrogen peroxide-induced oxidation. Anim. Sci. 84 (Suppl.1): 38. 68. Guimaraes, S. E. F., M. F. Rothschild, E. Huff-Lonergan, A. A. Sosnicki, S.B. Jungst, M.Yu, and S. M. Lonergan. 2006. Association analyses of MC4R and PRKAG3 genes in commercial pig population with different genetic potential for growth. World Congress of Genetics Applied in Livestock Production. Belo Horizonte, MG, Brazil. 69. Lonergan, S. M. K. J. Stalder, T. J. Knight, E. Huff-Lonergan, R. N. Goodwin, K.J. Prusa, and D. C. Beitz. 2006. Influence of lipid content on pork sensory texture and star probe within pH classification. Proceedings, 59th Reciprocal Meat Conference, June 18-22, 2006 - University of Illinois, Champaign-Urbana. 70. Lonergan, S. M., and E. Huff-Lonergan. 2006. Molecular factors that contribute to development of pale soft and exudative pork. Presented at IFT Annual Expo, June 24, 2006, Orlando, FL. 71. Wagner, C. E., E. Huff-Lonergan, M. F. Rothschild, A. A. Sosnicki, S. B. Jungst, K. J. Prusa, and S. M. Lonergan. 2006. Effects of selection for rapid growth on fresh pork quality. 2006 Allen D. Leman Swine Conference Proceedings. 72. Lonergan, S., K. Stalder, E. Huff-Lonergan, T. Knight, R.Goodwin, K. Prusa, D. Beitz. 2006. Classification of pork loin based on intramuscular lipid content to predict sensory and texture of fresh pork. J. Anim. Sci. 84:58-59. 73. Anderson , M.J., K. Mou, C. Gregorich, E. Steadham, S.M. Lonergan, and E. Huff-Lonergan. 2007. Proteolysis, calpastatin activity, and µ-calpain autolysis in specific muscles from the beef round. J. Anim. Sci. 85: (Suppl. 1) 74. Wagner, C.E., E. Huff-Lonergan, A.A. Sosnicki, S.B. Jungst, S.M. Lonergan. 2007. Effect of pig age at slaughter on postmortem muscle protein degradation and fresh pork quality. J. Anim.Sci. 85 (Suppl 1). 75. Knock, R.C., A.H. Trenkle, E.J. Huff-Lonergan, S.M. Lonergan, J.R. Russell, P.M. Dixon, K.M. Carnagey, and D.C. Beitz. 2007. Carcass and meat quality characteristics of distiller’s co-productsupplemented pasture and feedlot finished steers. J. Anim. Sci. 85 (Suppl.1) Elisabeth Huff Lonergan 22 76. Colecraft, E.K., G.S. Marquis, A.A. Lartey, O. Sakyi-Dawson, B. Ahunu, L.M. Butler, H.H. Jensen, M. B. Reddy, E. Lonergan. 2007 The magnitude and pattern of purchased ready-to-eat foods in the diets of rural Ghanaian children. FASEB Journal 21: A55-A55. 77. Wagner, C.E., E. Huff-Lonergan, A.A. Sosnicki, S.B. Jungst, S.M. Lonergan. 2007. Effect of sire Estimated Breeding Value (EBV) for growth rate on correlated pork quality traits. Proceedings, 60th Reciprocal Meat Conference, June 17-20 South Dakota State University, Brookings, SD. 78. Anderson, M.J., K. Mou, E. Steadham, C. Fedler, K. Prusa, S.M. Lonergan, and E. Huff-Lonergan. 2007. Round Muscle Profiling: Influence of aging on palatability of specific wholesale round cuts. Proceedings, 60th Reciprocal Meat Conference, June 17-20 South Dakota State University, Brookings, SD. 79. Knock, R.C., A.H. Trenkle, E.J. Huff-Lonergan, S.M. Lonergan, J.R. Russell, P.M. Dixon, K.M. Carnagey, D.C. Beitz. 2007 Use of 25-hydroxyvitamin D3 to improve tenderness of beef from distiller’s co-product-supplemented pasture- and feedlot-finished beef steers. Proceedings, 60th Reciprocal Meat Conference, June 17-20 South Dakota State University, Brookings, SD. 80. Anderson, M.J., S.M. Lonergan, E. Huff-Lonergan. 2007. Round muscle profiling of tenderness and postmortem proteolysis. J. Anim. Sci. 85:380-381 (Suppl. 1). 81. Lametsch, R., E. Huff-Lonergan, and S. M. Lonergan. 2008. Oxidation results in formation of an intramolecular disulfide bond in µ-calpain J. Anim. Sci.(E-supplement) 86:317 82. Zhang,W. S. M. Lonergan and E. Huff-Lonergan. 2008. Nitrosylation affects the autolysis of µcalpain. J. Anim. Sci.(E-supplement) 86:570. 83. Anderson, M. J., R. M. Smith, E. Huff-Lonergan, S. M. Lonergan. 2008. Correlation of pork texture characteristics determined using different instrumental texture analysis methods. J. Anim. Sci .(E-supplement) 86:318 84. R. M. Smith, M. J. Anderson, J. Viguera, E. Huff-Lonergan, S. M. Lonergan. 2008. Mapping quality attributes within the pork loin. J. Anim. Sci. J. Anim. Sci.(E-supplement) 86:570. 85. Anderson, M. J., E. Steadham, C. Fedler, K. Prusa, S. M. Lonergan, E. Huff-Lonergan. 2008. Influence of fiber type on palatability of beef round muscles. J. Anim. Sci.(E-supplement) 86:43. 86. Huff-Lonergan, E. and S.M. Lonergan. 2008. Relationship of postmortem changes in myofibrillar protein to meat quality. J. Anim. Sci.(E-supplement) 86:520-521. 87. Smith, R.M., J.M. Young, M.J. Anderson, R.C. Johnson, E. Huff-Lonergan, J. Dekkers, S.M. Lonergan. 2009. Influence of selection for reduced residual feed intake on pork quality. J. Anim Sci. 87 (E-Suppl. 3) 75. 88. Kim, Y. H., E. D. Steadham, S. M. Lonergan, E. Huff-Lonergan. 2009. Antioxidant capacity of calcium lactate on m-calpain activity in vitro J. Anim Sci. 87 (E-Suppl. 3) 75. Elisabeth Huff Lonergan 23 89. Zhang, W. S. M. Lonergan and E. Huff-Lonergan.2009. Protein nitrosylation in postmortem beef longissimus dorsi and psoas major muscles. J. Anim Sci. 87 (E-Suppl. 3) 78. 90. Binning, J. M., M. J. Anderson, S. M. Lonergan, E. Huff-Lonergan. 2009. Postmortem degradation of desmin in specific muscles from the beef round. J. Anim Sci. 87 (E-Suppl. 3) 134. 91. Marquis, G.S., Harding, K.B., Colecraft, E.K., Lartey, A., Sakyi-Dawson, O., Ahunu, B., Reddy, M.B., Jensen, H.H., Butler, L.M., (Huff-)Lonergan, E. 2009. Integrating economic and educational intervention activities leads to improved child nutritional status in rural Ghana. FASEB J. 92. Fritchen, N. , Kim, Y. H. , Hosch, J. J. Lonergan, S. M. Huff-Lonergan, E. 2010. Protein denaturing condition of beef inside semimembranosus muscle caused less protein degradation and µ-calpain autolysis than outside semimembranosus. J. Anim. Sci 88 (E-Supplement 3):144 93. Y. H. Kim, S. M. Lonergan, E. Huff-Lonergan. 2010. Lactate/phosphate injection enhancement improved oxidation stability and tenderness of postmortem beef cuts packaged in high oxygen modified atmosphere. J. Anim. Sci 88 (E-Supplement 3):79. 94. Kim, Y. H., S. M. Lonergan, and E. Huff-Lonergan. 2010. Influence of high-oxygen and lactate/phosphate enhancement on proteolysis, protein polymerization and tenderness of postmortem beef muscles. International Congress of Meat Science and Technology. August 16-20, 2010. South Korea. 95. Kim, Y. H., A. Fritchen, J. K. Grubbs, M. J. Anderson, S. M. Lonergan, and E. Huff-Lonergan. 2010. Protein denaturation of beef deep semimembranosus muscle negatively affects meat tenderness by limiting protein degradation and µ-calpain autolysis. International Congress of Meat Science and Technology. August 16-20, 2010. South Korea. 96. Grubbs, J.K., A.N. Fritchen, Y.H. Kim, S.M. Cruzen, S.M. Lonergan, and E. Huff-Lonergan. 2011. The use of calcium lactate to improve quality in beef round muscles in high-oxygen modified atmosphere packaging. Meat Science 89 335 97. Grubbs, J.K., A.N. Fritchen, Y.H. Kim, S.M. Lonergan, and E. Huff-Lonergan. 2011.Use of electrical stimulation to promote tenderness in the beef round muscles. J. Anim. Sci. 89 (E-Suppl 2):90. 98. Cruzen S.M., P.V. R. Paulino, E. Steadham, E. Huff-Lonergan and S. M. Lonergan. 2011. Calpain and calpastatin activity in porcine longissimus and the red and white portions of the semitendinosus. 57th International Congress of Meat Science and Technology, 7-12 August 2011, Ghent-Belgium. 99. della Malva, A., R. Marino, J. K. Grubbs, A. N. Fritchen, S. M. Lonergan, and E. Huff-Lonergan. 2011. Effect of low voltage electrical stimulation on changes in proteome of bovine longissimus muscle during postmortem aging. 57th International Congress of Meat Science and Technology, 712 August 2011, Ghent-Belgium 100. Huff-Lonergan, E. and S. M. Lonergan. 2011. The role of the muscle cell microenvironment on postmortem proteolysis. J. Anim. Sci. Vol. 89, (E-Suppl. 1):678. Punt, R. M., R.M. Smith, J.K. Grubbs, E. Huff-Lonergan, S. M. Lonergan. 2012. Relationship between Elisabeth Huff Lonergan 24 proteolysis and oxidation in longissimus dorsi pork loin chops. J. Anim. Sci. 90 (suppl.2):99. 101. Punt, R. M., R.M. Smith, J.K. Grubbs, E. Huff-Lonergan, S. M. Lonergan. 2012. Relationship between proteolysis and oxidation in longissimus dorsi pork loin chops. J. Anim. Sci. 90 (suppl.2):99. 102. Grubbs, J. K, A. N. Fritchen, A. Harris, E. Huff-Lonergan, N.K. Gabler, S.M. Lonergan. 2012. Reactive oxygen species production in mitochondria of pigs selected for residual feed intake. FASEB J. 26:887.5 103. Grubbs, J.K., A.N. Fritchen, A. Harris, E. Huff-Lonergan, N.K. Gabler, S.M. Lonergan. 2012. Protein profile in mitochondria of pigs selected for residual feed intake. FASEB J. 26:888.5 104. Gabler, J.K., N.K., Grubbs, A. Harris, S. Cruzen, E. Huff-Lonergan, J.F. Patience, J.C.M. Dekkers, S.M. Lonergan. 2012. The physiological bases defining differences in the efficiency of dietary energy utilization in pigs selected for residual feed intake. J. Anim. Sci. 90 (Suppl. 3):455 105. Paulino, P.V. R., S M. Cruzen, E. Steadham, T. Nerilson, S. M. Lonergan, and E. HuffLonergan. 2012. Calpastatin activity and muscle protein degradation in weanling calves and cull cows. Proceedings International Congress of Meat Science and Technology. Montreal, Canada. 106. Arkfeld, E. K., E. R. Benedict, R. C. Johnson, J. M. Young, J. F. Patience, J. C. M. Dekkers, N. K. Gabler, S. M. Lonergan, E. Huff-Lonergan. 2013. Divergent selection for residual feed intake impacts carcass composition of pigs on high or low energy diets. J. Anim. Sci. 91(Suppl.2):9. 107. Cruzen, S.M., P.V. R. Paulino, S. M. Lonergan, and E. Huff-Lonergan. 2013. Muscle protein degradation and activities of calpain and calpastatin in three muscles from growing and mature beef animals. Meat Science 93:35. 108. Arkfeld, E. K., E. R. Benedict, R. C. Johnson, J. M. Young, D. M. Thekkoot, J. K. Grubbs, S.M. Cruzen, N. K. Gabler, J. C. M. Dekkers, S. M. Lonergan, E. Huff-Lonergan. 2013. Composition and quality of carcasses from pigs selected for divergent residual feed intake. Meat Science 93:25. 109. Arkfeld, E. K., E. R. Benedict, R. C. Johnson, J. M. Young, D. M. Thekkoot, J. K. Grubbs, S.M. Cruzen, N. K. Gabler, J. C. M. Dekkers, S. M. Lonergan, E. Huff-Lonergan. 2013. Composition and quality of carcasses from pigs selected for divergent residual feed intake. Meat Science 93:25. 110. Cruzen, S. M., A. J. Harris, K. Hollinger, J. T. Selsby, N. K. Gabler, S. M. Lonergan and E. HuffLonergan. 2012. Gilts selected for low residual feed intake have potential for decreased protein degradation. Proceedings International Congress of Meat Science and Technology. Montreal, Canada. 111. Arkfeld, E. K., E. R. Benedict, R. C. Johnson, J. M. Young, J. F. Patience, J. C. M. Dekkers, N. K. Gabler, S. M. Lonergan, E. Huff-Lonergan. 2013. Divergent selection for residual feed intake impacts carcass composition of pigs on high or low energy diets. J. Anim. Sci. 91(Suppl.2):9. 112. Mohrhauser, D., S. Lonergan, E. Huff-Lonergan, K. Underwood, A. Weaver. 2013. Investigation of protease activity in early post- mortem muscle subjected to alternative chilling conditions. J. Anim. Sci. 91(Suppl.2):101. 113. Arkfeld, E.K., E. R. Benedict, R. C. Johnson, J. M. Young, J. F. Patience, J.C.M. Dekkers, N. K. Gabler, S. M. Lonergan, and E. Huff-Lonergan. 2014. Effects of divergent selection for residual feed intake and diets varying in energy and fiber content on pork loin protein degradation and sensory quality. Meat Science 96:470(doi:10.1016/j.meatsci.2013.07.102). Elisabeth Huff Lonergan 25 114. Punt, R. M. A. M. Blakely, J. K. Grubbs, S. M. Cruzen , E. Huff-Lonergan, S. M. Lonergan. Relationship between residual feed intake, protein oxidation and protein degradation in pigs. Meat Science (Submitted) 96:470-471 (doi:10.1016/j.meatsci.2013.07.104). 115. Cruzen, S.M., S. M. Lonergan, E. Huff-Lonergan. 2013. Analysis of calpastatin pools separated using ion exchange chromatography. Meat Science 96:491-492 (doi:10.1016/j.meatsci.2013.07.150). 116. Grubbs, J.K., N.K. Gabler, E. Huff-Lonergan, and S.M. Lonergan. 2013. Posttranslational modification differences in the mitochondria protein profile of pigs selected for low and high residual feed intake. Meat Science Meat Science 96:492-493 (doi:10.1016/j.meatsci.2013.07.152). 117. Bigi, M., J. K. Grubbs, E. Huff-Lonergan, K. J. Prusa, R. Davoli, S. M. Lonergan. 2014. Changes in pork loin sarcoplasmic protein profile in response to chilling method. Meat Science (In Press). 118. Arkfeld, E.K., J. E. Berger, E. R. Hamman, J. M. Young, R. C. Johnson, C. A. Fedler, K. Prusa, J. F. Patience, J. C. M. Dekkers, N. K. Gabler, S. M. Lonergan, E. Huff-Lonergan. 2014. Effect of diet and selection for residual feed intake on sensory quality and postmortem proteolysis of pork loin. Meat Science (In Press). 119. Arkfeld, E. K., J. E. Berger, J. M. Young, R. C. Johnson, J. F. Patience, J. C. M. Dekkers, N. K. Gabler, S. M. Lonergan, E. Huff-Lonergan. 2013. Low energy-high fiber diets increase iodine values in pigs irrespective of residual feed intake selection. Meat Science (In Press) 120. Blakely, A., G. B. Sherrard, K. J. Prusa, C. A. Fedler, E. K. Arkfeld, E. M. Steadham, K. D. Forsythe, E. Huff-Lonergan, R. M. Punt, T. Goodman, S. M. Lonergan. 2014. Effects of postmortem rapid chilling on sensory attributes of fresh pork cuts from the longissimus dorsi, poas major, triceps brachii, and semimembranosus. Meat Science (In Press) 121. Arkfeld E. A., J. E. Berger, E. R. Hamman, J. M. Young, R. C. Johnson, J. F. Patience, J. C. M. Dekkers, N. K. Gabler, S. M. Lonergan, E. Huff-Lonergan. 2014. Pork quality characteristics of loins from pigs divergently selected for residual feed intake on high or low energy diets. Articles in Non-Refereed Publications Extension Reports 1. Chiba, L.I., D.L. Kuhlers, L.T. Frobish, S.B. Jungst, E.J. Huff- Lonergan, S.M. Lonergan, and B. L. Anderson. 1999. Amino acid restrictions and pigs selected for lean growth efficiency. Auburn University Animal Agricultural Conference Proceedings. 2. Huff-Lonergan, E., S.M. Lonergan, S.E. Harris, D.L. Kuhlers, S.B. Jungst. 1999. Genetic factors influencing pork quality. Auburn University Animal Agricultural Conference Proceedings. 3. Harris, S.E., E. Huff-Lonergan, S.M. Lonergan, D.L. Rankins, W.R. Jones. 1999. Color stability and tenderness properties of beef from vitamin E fed cattle. Auburn University Animal Agricultural Conference Proceedings. 4. Lonergan, S.M., E. Huff-Lonergan, and L.A. Kriese-Anderson. 1999 Molecular factors influencing beef tenderness. Auburn University Animal Agricultural Conference Proceedings. 5. Lonergan, S., E. Huff-Lonergan, G. Rouse, L.Vaske. 2000. Biochemical indicators of beef tenderness. Rhodes Research Farm Annual Report. 6. Ciobanu, D.C., S. M. Lonergan, M. Malek, J.R. Woolard, E.J. Lonergan, and M.F. Rothschild. 2002. New alleles in the calpastatin gene associated with improved tenderness in pork. Iowa State University Swine Report. 7. Ciobanu, D.C., S.M. Lonergan, M. Malek, J.R. Woollard, E.J. Lonergan, M.F. Rothschild. 2002. New Alleles in the Calpastatin Gene Associated with High Tenderness in Pigs. Iowa State University Swine Report. Elisabeth Huff Lonergan 26 8. Davis, K. J., J. G. Sebranek, E. Huff-Lonergan and S. M. Lonergan. 2004. Effect of aging on moisture enhanced pork loins. AS Leaflet. 9. Gardner, M. A., E. Huff-Lonergan and S. M. Lonergan. 2004. Influence of harvest processes on pork loin and ham quality. AS Leaflet. 10. Carnagey, K.M., E.J. Huff-Lonergan, R.L. Horst, A.H. Trenkle, D.C. Beitz. 2004. Use of 25-hydroxyvitamin D3 to improve tenderness of beef. AS Leaflet. 11. Huff-Lonergan E., S. M. Lonergan and L. Rowe. 2005. Irradiation-Induced Protein Oxidation Influences Meat Tenderness. Feedinfo News Service Scientific Reviews. July 2005. Available from URL: http://www.feedinfo.com. 12. Maddock, K. R., E. Huff-Lonergan, and S. M. Lonergan. 2005. The effect of pH on µ-calpain activity and implications in meat tenderness. AS Leaflet 1988. 13. Mendez, G., E. Huff-Lonergan and S. M. Lonergan. 2005. Processing factors influence fresh pork color and water holding capacity. AS Leaflet 1989. 14. Patton, B. S., E. Huff-Lonergan, M. Honeyman, and S. M. Lonergan. 2006. The effect of space allocation in hoop structures on swine performance and pork quality. ISU Animal Industry Report. A. S. Leaflet 2161. 15. Patton, B. S., E. Huff-Lonergan, M. Honeyman, and S. M. Lonergan. 2006. Effects of finishing pigs in hoop structures on swine performance, pork composition and pork quality. ISU Animal Industry Report. A. S. Leaflet 2162. 16. S. M. Lonergan, R. Goodwin, E. Huff-Lonergan, K. Stalder, K. J. Prusa, T. Knight and D. Beitz. 2006. Classification of pork loin based on lipid composition to predict sensory and textural properties. ISU Animal Industry Report. A. S. Leaflet 2052 17. Carnagey, K. M., E. J. Huff-Lonergan, S. M. Lonergan, R. L. Horst, A. H. Trenkle, and D. C. Beitz. 2006. Use of 25-hydroxyvitamin D3 and dietary calcium manipulations to improve tenderness of beef. ISU Animal Industry Report. A. S. Leaflet 2055. 18. Lonergan, S. M., K. Stalder, E. Huff-Lonergan, R. Goodwin, K. J. Prusa, T. Knight and D. Beitz. 2007. Contributions of pH and intramuscular lipid to sensory quality of fresh pork loin. ISU Animal Industry Report A.S. Leaflet 2241. 19. Knock, R., A. Trenkle, D. Beitz, E. Huff-Lonergan, S. Lonergan, J. Russell. 2007. Comparison of gain and carcass traits from pasture- and feedlot-finished beef steers supplemented with distillers’ grain co-products. ISU Western Research and Demonstration Farm Report. 20. Anderson, M. J., K. Mou, E. Steadham, C. Fedler, K. Prusa, S. M. Lonergan, and E. Huff-Lonergan. 2008. Round muscle profiling: Influence of aging on the palatability of specific wholesale round cuts. ISU Animal Industry Report, AS Leaflet R2266. 21. Anderson, M. J., S. M. Lonergan, and E. Huff-Lonergan. 2008. Round muscle profiling: Management of tenderness and sensory improvement of specific muscles with aging. ISU Animal Industry Report, AS Leafet R2265. 22. Wagner, C., E. Huff-Lonergan, A. Sosnicki, and S. M. Lonergan. 2008. Effect of pig age at slaughter on fresh pork quality. ISU Animal Industry Report, AS Leaflet R2260. 23. Lonergan S. M., E. Huff-Lonergan, J. G. Sebranek. 2008. Undergraduate minor in meat science. ISU Animal Industry Report, AS Leaflet R2367. 24. Anderson, M. J., E. Steadham, C. Fedler, K. J. Prusa, S. M. Lonergan, E. Huff-Lonergan.2009. Influence of Fiber Type on Palatability Attributes of Beef Round. ISU Animal Industry Report, AS Leaflet R2393 25. Anderson, M. J., J. Binning, S. M. Lonergan, E. Huff-Lonergan. 2009. Postmortem Degradation of Desmin in Specific Muscles from the Beef Round. ISU Animal Industry Report, AS Leaflet R2395 26. Kim, Y. H., E. Steadham, S. M. Lonergan, E. Huff-Lonergan. 2009. Antioxidant capacity of calcium lactate on m-calpain activity In Vitro. ISU Animal Industry Report, AS Leaflet R2396 Elisabeth Huff Lonergan 27 27. Smith, R. M., J. Young, M. J. Anderson, R. Johnson, E. Huff-Lonergan, J.C.M. Dekkers, S. M. Lonergan. 2009. Effect of selection for reduced residual feed intake on pork carcass composition and fresh pork quality. ISU Animal Industry Report, AS Leaflet R2394 28. Kim, Y. S. M. Lonergan, E. Huff-Lonergan. 2010. High oxygen packaging system negatively affects color stability and sensory attributes of beef cuts. ISU Animal Industry Report, AS Leaflet-R2489 29. Anderson, M. J., T. Grevengoed, S. M. Lonergan, and E. Huff-Lonergan. 2010. Proteomic Analysis of Bovine Muscles during Aging. ISU Animal Industry Report, AS Leaflet-R2490 30. Kim, Y. H., S. M. Lonergan, E. Huff-Lonergan. 2010. Lower oxygen or addition of antioxidants: High oxygen MAP system diminishes quality through oxidation. FLEISCHWIRTSCHAFT International. No. 1. 2010:30-31. 31. Arkfeld, E., E. Benedict, R. Johnson, J. Young, J. Patience, J. Dekkers, N. Gabler, S. Lonergan, and E. Huff-Lonergan. Composition and quality characteristics of pigs selected for divergent RFI on high or low energy diets. ISU Animal Industry Report, AS Leaflet 2767. 32. Arkfeld, E., E. Hamman, J. Berger, R. Johnson, J. Young, C. Fedler, K. Prusa, J. Patience, J. Dekkers, N. Gabler, S. Lonergan, E. Huff-Lonergan. 2014. Sensory Characteristics of Loins from Pigs Divergently Selected for Residual Feed Intake and Fed Diets Differing in Energy. AS-Leaflet R289. 33. Arkfeld, E., E. Hamman, J. Berger, R. Johnson, J. Young, C. Fedler, K. Prusa, J. Patience, J. Dekkers, N. Gabler, S. Lonergan, E. Huff-Lonergan. 2014. Diets Differing in Energy and Fiber Content Affected Adipose Tissue Quality of Carcasses from Pigs Divergently Selected for Residual Feed Intake. AS-Leaflet 2905. 34. Arkfeld, E., E. Hamman, J. Berger, R. Johnson, J. Young, C. Fedler, K. Prusa, J. Patience, J. Dekkers, N. Gabler, S. Lonergan, E.Huff-Lonergan. 2014. Divergent Selection for Residual Feed Intake Impacts Carcass Composition of Pigs on High or Low Energy Diets. AS-Leaflet 2906. 35. Arkfeld, E., E. Hamman, J. Berger, R. Johnson, J. Young, C. Fedler, K. Prusa, J. Patience, J. Dekkers, N. Gabler, S. Lonergan, E.Huff-Lonergan. 2014. The Impact of Divergent Selection for Residual Feed Intake on Meat Quality Traits of Loins from Pigs on High or Low Energy Diets. AS-Leaflet 2907. 36. Cruzen, S. R. Boddicker, K.Graves, T. Johnson, E. Arkfeld, L. Baumgard, J. Ross, S.Lonergan, T. Safranski, M. Lucy. 2014. Carcass Composition of Market Weight Pigs Subjected to Heat Stress in utero or during Growth. AS-Leaflet 2921. 37. Grubbs, K., N. Gabler, J. Dekkers, E. Huff-Lonergan, S. Lonergan. 2014. Selection for Residual Feed Intake Alters the Protein Profile of the Mitochondria in Pigs. AS-Leaflet 2923. Articles in Trade Publications 1. Huff-Lonergan, E., and J. Page. 2000. Chilling and Pork Quality. Meat Processing. August 2000. pp 54-59. 2. Huff-Lonergan E., S. M. Lonergan and L. Rowe. 2005. Irradiation-Induced Protein Oxidation Influences Meat Tenderness. Feedinfo News Service Scientific Reviews. July 2005. Available from URL: http://www.feedinfo.com. Elisabeth Huff Lonergan 28 Training Modules and Fact Sheets for the Meat Industry 1. Huff-Lonergan and J.K. Page. 2000. Effects of Chilling on Pork Quality. American Meat Science Association. 2. Page, J.K., E. Huff-Lonergan, and D. Meisinger. 1999. Chilling: Impact on Pork Quality. Annotated slide set/PowerPoint presentation designed for education and training of meat industry professionals and students. Summarizes the National Pork Producers Chilling Workshop held in November, 1999. 3. Huff-Lonergan, E. 2000. Pork Quality. Annotated PowerPoint presentation designed for education and training of meat industry professionals and students. Like the updated version (2003), this PowerPoint presentation was provided to all presenters of Pork 101 conferences across the nation. Copies of these slides were made available to all participants in Pork 101 conferences nationwide. Annually, this talk was presented as many to as many as 500 meat industry professionals attending at lest one of 8 conferences held nationwide. Since this version was used for 3 years (2000, 2001, 2002) a minimum of 1500 pork industry professionals were reached with this presentation nationwide. 4. Huff-Lonergan, E. 2003. Pork Quality. Annotated PowerPoint presentation designed for education and training of meat industry professionals and students. This PowerPoint presentation was updated to include information on the influence of genetics on fresh pork quality. Like the first edition, this presentation has been provided to all presenters of Pork 101 conferences across the nation. Copies of these slides are made available to all participants in Pork 101 conferences nationwide. Annually, this talk will be presented as many to as many as 300 meat industry professionals attending at least one of 4 conferences held nationwide. 5. Huff-Lonergan, E. 2003. Water-Holding Capacity of Fresh Meat – Fact Sheet. American Meat Science Association and National Pork Board. Invited Presentations 1. Parrish, F.C., Jr., E.J. Huff, and R.D. Johnson. 1992. Postmortem technologies to reduce meat quality variation. American Society of Animal Science Annual Meeting. Aug. 1992. 2. Huff, E.J., D.D. Beekman, and F.C. Parrish, Jr. 1993. Protein separation and analysis: Principles and techniques. Institute of Food Technologists Annual Meeting Basic Symposium. June, 1993. 3. Huff-Lonergan, E.J. 1994. Review of calpain/calpastatin system in muscle and meat. Iowa State University Animal Science Departmental Seminar. Spring, 1994. 4. Huff-Lonergan, E.J. 1996. Poster Summary-Muscle Tenderness Poster Session. 49th Annual Reciprocal Meat Conference. June 11, 1996. 5. Huff-Lonergan, E.J. 1996. Pork Quality: Current issues and research. Auburn Swine Conference. November 15, 1996. 6. Huff-Lonergan, E.J. 1997. Methods to monitor/quantify postmortem changes in the muscle proteins. 50th Annual National Meeting of the Reciprocal Meat Conference. Ames, IA. July 1, 1997. 7. Huff-Lonergan, E.J. 1997. Postmortem strategies to improve beef tenderness. Alabama Cattlemen’s Summer Meeting. July 23, 1997. Elisabeth Huff Lonergan 29 8. Huff-Lonergan, E.J. 1997. The calpains : Structure, function and utility in food animal production. Auburn University Reproductive Biology Forum. May 20, 1997. 9. Huff-Lonergan, E.J., S.M. Lonergan, and D.L. Kuhlers. 1998. Identification of biological mechanisms influencing pork quality and efficient production of pork in the absence of the Halothane gene. Presented to the National Pork Producers Council Pork Quality Solutions Committee. Des Moines, IA. July, 1998. 10. Huff-Lonergan, E.J., and S.M. Lonergan 1998. Postmortem mechanisms of meat tenderization: The roles of the structural proteins and the calpain system. American Chemical Society, Agricultural and Food Chemistry Division. Symposium: Quality attributes of muscle foods. Dallas TX. March 29-April 2, 1998. 11. Huff-Lonergan, E.J. 1999. Factors influencing pork tenderness. National Pork Producers’ Council Targeted Pork Quality Research Initiative Meeting. Nov. 12, 1999. 12. Huff-Lonergan, E.J. 1999. Pork Quality. National Pork Producers’ Council. Presented to Japanese Media Representatives. Oct. 13. 1999. 13. Huff-Lonergan, E.J. 1999. Possible role and regulation of the calpain/calpastatin system in meat quality. American Society of Animal Science National Meeting. Symposium: Roles of the Calpain/Calpastatin System in Muscle Growth and Meat Quality. Indianapolis, IN. July 21-23, 1999. 14. Huff-Lonergan, E.J., S.M. Lonergan, S.B. Jungst and D.L. Kuhlers. 1999. Selection for lean growth efficiency: Impact on pork quality. 52nd Annual Reciprocal Meat Conference. June 20-23, 1999. 15. Huff-Lonergan, E., and J. Page. Chilling effects on pork quality. Pork Quality and Safety Summit. July 12, 2000. Des Moines, IA. 16. Huff-Lonergan, E.J. 2000. Tenderness. Presented at a workshop entitled, “pH: An objective predictor of pork quality”. Sponsored by the American Meat Science Association and the National Pork Producers Council. June 22, 2000. Columbus OH. 17. Huff-Lonergan, E.J. Meat Science at Iowa State University. University of Nebraska Meat Science Seminar Invited Speaker, April 10, 2000. Lincoln, NE. 18. Huff-Lonergan, E.J., 2000. The role of protein oxidation in calpain inhibition: Can we unlock the secrets of the calpain system? Muscle Biology Seminar, Spring 2000. 19. Huff-Lonergan, E.J. 2000. Postmortem regulation of the calpain system. Animal Science Department Spring Seminar. March 10, 2000. 20. Huff-Lonergan, E., A. Asmus, and S.M. Lonergan. 2002. Antioxidants and Meat Quality: Novel approaches to understanding water-holding capacity and tenderness. Pork Quality and Safety Summit. June 18, 2002. Des Moines, IA. Elisabeth Huff Lonergan 30 21. Huff-Lonergan, E., S.M. Lonergan, J. Dodge, and L. Rowe. 2002. Biochemical Aspects of Meat tenderness. Workshop on Pork Measures. American Meat Science Association and the National Pork Board. August 1, 2002. East Lansing Michigan. 22. Huff-Lonergan, E. 2004. Structural changes influencing meat tenderization and water-holding capacity. April 28, 2004. Meat Science Graduate Students. University of Nebraska, Lincoln, NE 23. Huff-Lonergan, E. 2004. Recent developments in calpain regulation: Are they keys to understanding variation in meat quality? April 28, 2004. Animal Science Department, University of Nebraka, Lincoln, NE 24. Huff-Lonergan, E., S.M. Lonergan, A. Ostendorf, and R. Klont. 2004. Impact of proteolysis on pH decline and water-holding capacity of fresh meat. National Pork Board Pork Quality and Safety Summit. August, 17, 2004. Des Moines, IA 25. Huff-Lonergan, E., S.M. Lonergan, and A. Ostendorf. 2004. Role of muscle cytoskeletal and intermediate filament proteins in the development of soft and exudative pork. National Pork Board Pork Quality and Safety Summit. August, 18, 2004. Des Moines, IA 26. Huff-Lonergan, E. 2005. Mechanisms of water-holding capacity of meat. 51st International Congress of Meat Science Technology. August 12, 2005. Baltimore, MD. 27. Huff-Lonergan, E., and S.M. Lonergan. 2005. New frontiers in understanding drip loss: recent insights on the role of postmortem muscle biochemistry. Workshop “Drip Loss and Water Holding Capacity of Porcine Meat”. University of Bonn, Germany. December 5-6. 28. Lonergan S. M., E. Huff-Lonergan and A. K. Johnson. 2006. Influence of swine handling on pork quality. International Meat Animal Welfare Conference. Sponsored by American Meat Institute, American Meat Science Association and the Federation of Animal Science Societies. Kansas City MO. February 22, 2006. 29. Lonergan S. M., E. Huff-Lonergan and A. K. Johnson. 2006. How does swine handling influence pork quality and value? . American Meat Institute Animal Care and Handling Conference. Kansas City MO. February 23, 2006 30. Lonergan S. M., and E. Huff-Lonergan. 2006. Molecular factors that contribute to development of pale soft and exudative pork. Institute of Food Technologists Annual meeting. Orlando FL. June 24, 2006. 31. Lonergan S. M. and E. Huff-Lonergan. 2006. Molecular factors that contribute to variation in water holding capacity in pork. Finland National Meat Symposium. October 19, 2006. 32. Huff-Lonergan, E., and S.M. Lonergan. 2006. Crossing the line between muscle and meat: The influence of muscle cell physiology on meat quality. Symposium : Components and practical aspects of meat quality Midwest American Society of Animal Science/American Society of Dairy Science Annual Meeting. Des Moines, IA March 21, 2006. 33. Huff-Lonergan, E. 2006. Recent developments in calpain regulation: Are they keys to understanding variation in meat quality? Meat Science Seminar - Animal Science Department, University of Nebraska, Lincoln, NE. April 10, 2006. Elisabeth Huff Lonergan 31 34. Huff-Lonergan, E. 2006. Structural changes influencing meat tenderization and water-holding capacity. Meat Science Graduate Students. University of Nebraska, Lincoln, NE April 10, 2006. 35. Huff-Lonergan, E., S.M. Lonergan, and K Maddock Carlin. 2007. New insights on calpain regulation in skeletal muscle. FASEB Conference: The Biology of Calpains in Health and Disease. Held July 14-19, 2007, Snowmass, CO. Presented July 19, 2007. 36. Weaver, A., S.M. Lonergan. E. Huff-Lonergan. 2007. Techniques in gel electrophoresis and western blotting. Proc. Recip. Meat Conf. (on-line publication) http://www.meatscience.org/Pubs/rmcarchv/2007/index.html 37. Huff-Lonergan, E., S.M. Lonergan, K. Maddock Carlin. 2007. New insights on calpain regulation in postmortem skeletal muscle. FASEB Summer Research Confernce. “Biology of Calpain in Health and Disease”. Snowmass Village, Colorado. July 19, 2007 38. S.M. Lonergan and E. Huff-Lonergan. 2007. The role of muscle physiology on meat quality. Beijing Agricultural University. Beijing, China. August 8, 2007. 39. E. Huff-Lonergan. 2007 Ghana – Enhancing child nutrition through Animal Resource Food Management (ENAM): An animal scientitst’s perspective of the role of animal source foods in the diets of young children in rural Ghana. Fall 2007 Ensminger International Program Seminar Series. September 20, 2007. 40. E. Huff-Lonergan and S.M. Lonergan. 2007. The influence of muscle structure on the waterholding capacity of fresh meat. To be presented at the Meat Research Institute in Roskilde, Denmark on November 28, 2007. 41. E. Huff-Lonergan and S.M. Lonergan. 2007. New insights on calpain regulation in postmortem muscle; Impacts on meat quality. To be presented to the Department of Food Science, University of Copenhagen, Denmark on November 30, 2007. 42. Lonergan, S. M., E. Huff-Lonergan, B. Kerr, and K. J. Prusa. 2008. Contribution of fatty acid composition to fresh meat quality. Midwest ASAS/ADSA meeting March 2008. Des Moines IA. 43. E. Huff-Lonergan and S.M. Lonergan. 2008. Relationship of postmortem changes in myofibrillar protein to meat quality. Centennial Meeting of the American Society of Animal Science, Indianapolis, Indiania, July 7-11, 2008. 44. Huff-Lonergan, E. and S.M. Lonergan 2008. Interaction between the muscle contraction system and proteolytic tenderization in beef. International Congress of Meat Science and Technology. Cape Town, South Africa. August 10-15, 2008. 45. Huff-Lonergan, E., Zhang, W., Lonergan, S.M. 2010. Biochemistry of postmortem muscle – Lessons on mechanisms of meat tenderization. Internatioal Congress of Meat Science and Technology - JeJu Island, South Korea August 2010. 46. Huff-Lonergan, E., M.J. Anderson, and S.M. Lonergan. 2011. Proteomic applications for meat tenderness. American Meat Science Association. 64th Reciprocal Meats Conference, Manhattan KS. June 2011. Elisabeth Huff Lonergan 32 47. Weaver, A., S.M. Lonergan. E. Huff-Lonergan. 2007. Techniques in gel electrophoresis and western blotting. Proc. Recip. Meat Conf. 48. Huff-Lonergan, E. M. J. Anderson, and S. M. Lonergan. 2011. Proteomic applications for meat tenderness. American Meat Science Association. 64th RMC Proceedings. 49. Huff-Lonergan, E., S.M. Lonergan, S. Cruzen. 2013. Biochemisty of muscle: Relevance of postmortem changes to aging. FASEB Summer Research Conference Proceedings. “Biology of Calpain in Health and Disease”. Saxtons River Vermont. July 25, 2013 Elisabeth Huff Lonergan 33 Teaching (25% of Appointment) Courses Taught – Auburn University Animal and Dairy Science 110 (Auburn University) - “Orientation to Animal and Dairy Science” 1 credit - 1997. Freshman level class that serves as an introduction to departmental programs and personnel, describes job opportunities for individuals trained in Animal Sciences. Guest lecturer on the muscle biology and meat science curriculum and career opportunities. (Dr. Dale Coleman - class coordinator) Animal and Dairy Science 370 (Auburn University) “Meat Science” 4 credits. - 1996, 1997, 1998. Approximately 40 students per year. Biochemical and physiological factors affecting fresh meat and processed meat quality. Fundamentals of ante- and postmortem muscle biology, slaughter, processing, storage and merchandising of meat and meat products. (Responsibilities -100% - Instructor) Animal and Dairy Science 490 (Auburn University) “Special Problems” 3 credits. - 1997. Co-directed (with Dr. Steven M. Lonergan) a senior undergraduate student majoring in Molecular Biology at Auburn University in a research project involving purifying and characterizing three monoclonal antibodies that were developed by our laboratories. Animal and Dairy Science 671 (Auburn University) - “Advanced Meat Science” 5 credits - 1996, 1998. Approximately 8 students per year. Revised and updated this graduate level course. The course covers muscle microanatomy, biochemistry of muscle proteins and lipids, biochemistry of skeletal muscle contraction, lipid/protein interactions, antemortem and postmortem factors affecting fresh and processed meat quality. Class Format - Formal lectures by the instructors, student and instructor led discussions of current literature, individual student development of research proposals. Co-taught with Dr. Steven M. Lonergan (1996, 1998) and Dr. William B. Mikel (1996). [Responsibilities - 40% of the lectures 1996 and 50% of the lectures in 1998 and class coordinator (1996 and 1998)] Animal Science 645/Chemistry 645 - dual listed. (Auburn University) - “Biochemical Research Techniques” 5 credits - 1996. Graduate level survey of modern biochemical laboratory techniques. Class format consists of lectures covering theoretical aspects of the techniques and laboratory exercises demonstrating the application of specific techniques. Guest lecturer on immunoblotting techniques. (Dr. Frank [Skip] Bartol -class coordinator). Muscle Biology/Meat Science Journal Club (Auburn University) - 1997. Established and coordinated a weekly journal club in the Animal and Dairy Science Department at Auburn University. Elisabeth Huff Lonergan 34 Courses Taught – Iowa State University Animal Science 211 (Iowa State University) taught for two semesters – “Issues Facing Animal Science” 1 credit. Spring 2000 and Spring 2001. 80 students/40 per section both semesters. Dr. Huff Lonergan oversaw one section both semesters. Spring 2001 she coordinated the class. This course presents an overview of the factors that define contemporary ethical and scientifically based issues facing animal agriculture. The course also promotes life skill development (including interaction skills, communication ability, organization, information gathering and leadership skills) emphasized in the context of issues study. (Responsibilities – 50% - shared with Dr. Jack Dekkers as Co-Instructor in 2000, 75% responsibility Co-Instructor with Dr. David Topel ; Huff-Lonergan was course coordinator in 2001) Animal Science 360 (Iowa State University) taught for 10 semesters – “Fresh Meats” 3 credits. Fall 1999. 12 junior and senior level animal science students; Fall 2000 16 junior and senior level animal science students. Fall 2001 20 junior and senior level animal science students. Fall 2002 16 junior and senior level animal science students, 2 Meat Science Ph.D. students and 1 Meat Science M. S. student also audited the class. Fall 2003 15 junior and senior level animal science students, 1 Ph.D. student and 1 visiting scientist sat in on the class. Fall 2004. 16 junior and senior level students from animal science, agricultural education, agricultural studies and health and human performance, 1 animal science M.S. student sat in on the class. Fall 2005 (co-taught with Dr. Dennis Olson who was preparing to take the class over). 11 junior and senior level students from animal science, agricultural education, agricultural studies. Fall 2012 – 13 students (12 undergraduate students, 1 graduate student). Fall 2013 – 23 students (22 undergraduate students and 1 graduate student). Fall 2014 – 17 students (16 undergraduate students, 1 graduate student)This course covers the impact of muscle structure, composition, rigor mortis, inspection, fabrication, handling, packaging and cooking on the palatability, nutritional value, yields, market value, and safety of fresh meat. Includes preparation and presentation of a research paper by the students. (Responsibilities -100% - Instructor) Animal Science 345– (Iowa State University) – “Animal Growth and Development” – 3 credits. Spring 2005. Dr. Huff-Lonergan was responsible for 2 lectures covering the role of muscle growth in meat quality. Animal Science 324x – “Food processing and nutrition for carnivorous companion animals”. 3 credits. Taught for 3 semesters (2011, 2013, 2014). Taught for the first time in Fall 2011-7 students. (responsible for 70% of the lectures and for course development, design and management. Lance Baumgard taught 30% of the lectures). Fall 2013 – 13 students. (responsible for 70% of the lectures and for course development, design and management. Cheryl Morris taught 30% of the lectures). Fall 2014 -13 students – Responsible for managing the course. Taught 30 % of the lectures, Dr. Kurt Rosentrater – ABE taught 30)% of the lectures and Dr. Cheryl Morris taught 40% of the lectures) Topics covered include meat processing and meat preservation for companion animal diets, regulatory standards, cutting edge technologies for processing meat/diets for companion animals, dietary needs of carnivorous companion animals and the effects of different processing methods on safety and nutrient bioavailability. Animal Science 451 – (Iowa State University) – “Animal Molecular Biology” 3 credits Fall 1999, 2000, 2001, 2002, 2004. Guest lecturer on SDS-PAGE and immunoblotting techniques. Animal Science 490 - (Iowa State University) – “Independent Study in Meat Science” 3 credits. Spring 2000. 1 senior Animal Science student. Directed a senior undergraduate student in an independent laboratory project in meat science/muscle biology. (Responsibilities -100% - Instructor) Elisabeth Huff Lonergan 35 Animal Science 590 - (Iowa State University) – “Special Topics in Animal Science” 3 credits. Fall 1999. 2 Ph.D students. Fall 2003. 1 Meat Science Ph.D. student and 2 Meat Science M. S. students. Directed the study of graduate students on muscle structure, composition, rigor mortis, inspection, fabrication, handling, packaging and cooking on the palatability, nutritional value, yields, market value, and safety of fresh meat. (Responsibilities -100% - Instructor) Animal Science 590. (Iowa State University) – “Special Topics in Animal Science” 2 credits. Spring 2002. 1 M.S. student in Ruminant Nutrition. Directed the study of a master's level student on muscle structure, composition, and rigor mortis. (Responsibilities – 100% - Instructor) Food Science/Human Nutrition 590C (Iowa State University) - "Supervised Teaching" Fall 2002 2012, 2013, 2014). Guided a Food Science and Animal Science graduate students in teaching the laboratory portion of Animal Science 360 "Fresh Meats". (Responsibilities -100% - Instructor) Food Science/Human Nutrition 613 (Iowa State University) “Food Proteins”. 3 credits. Fall 2005. 10 students. Taught the lectures and gave the exam over meat proteins. Animal Science 684 (Iowa State University) “Meat Science Seminar.” 1 credit. Spring 2003, 2004, 2005, 2006 Coordinated the graduate seminar. This involves grading written abstracts/summaries of their presentations, working with the students prior to their “public” presentation, grading the final presentation, and arranging for other speakers to fit around a “theme”. The spring 2003 theme was centered on laboratory techniques and their applications. Several speakers were from outside the meat science discipline. (Responsibilities -100% - Instructor) Animal Science 570 – (Iowa State University) – “Applied Muscle Biology” 3 credits. Instructor Spring 2005. 14 M.S. and Ph.D. students in animal science. Spring 2007. 8 M.S. and Ph.D. students in Animal Science. Spring 2008., 5 M.S. and 3 Ph.D. students in Animal Science. Spring 2010. Spring 2012, 9 students. Spring 2014 – 7 students. Course Description: Ante and postmortem factors impacting composition, structure, and chemistry of red meat and poultry muscle/meat, the conversion of muscle to meat, and the sensory and nutritional attributes of fresh meats. Oral research reports and a research proposal. (Responsibilities -100% - Instructor) Supervision of Graduate Students and Postdoctoral Researchers Supervision of Graduate Students at Auburn University Graduate Students Advised or Co-Advised Student Scott Edward Harris Degree /Date M.S. Spring 1999 Major Role Placement Meat Science Co-Advisor Product Development Specialist – Cryovac Sealed Air, Duncan, S.C. Graduate Student Committees at Auburn University Student Eru Liu Degree M.S. – Summer 1997 Major/Advisor Muscle Biology/Don Mulvaney Susan Gaasch M.S.- Summer 1999 Nutrition/Keith Cummins Elisabeth Huff Lonergan 36 Supervision of Postdoctoral Researchers at Iowa State University Researcher Period of time at ISU Project area Dr. Yuan (Brad) Kim September 2008 – May Novel methods to prevent protein oxidation in 2010 postmortem muscle Current Position – Asst. Professor, Purdue University Supervision of Graduate Students at Iowa State University Graduate Students Advised or Co-Advised Student Degree/ Date M.S. Fall 2001 Major Role Placement Meat Science Co-Advisor Product Development Specialist – Burke Manufacturing, Nevada IA Jamie Dodge Melody M.S. Summer 2002 Meat Science Advisor Research Scientist –College of Veterinary Medicine - Iowa State University Laura Rowe M.S. Fall 2002 Meat Science Advisor Senior Research Associate Pioneer Aaron Asmus M.S. Summer 2003 Meat Science Advisor Director of Research and Development – Jennie-O Products, Willmar MN Abby Ostendorf M.S. Spring 2004 Meat Science Advisor Product Applications Specialist Elanco, Greenfield, IN Graciela Mendez M.S. Spring 2005 Meat Science Co-Advisor Product Applications Specialist Kerry Ingredients, Chicago, IL Wan-Gang Zhang Ph.D. Received Meat Science Advisor Associate Professor – Nanjing Agricultural University Kathy Hadley Davis Elisabeth Huff Lonergan 37 Spring 2009 Assistant Professor of Animal Science –Sam Houston State University Ph.D. Spring 2011 Meat Science (Minor in Statisitcs) Advisor Shannon Cruzen Ph.D. Summer 2013 Meat Science (Minor in Statistics) Advisor Postdoctoral Research Assistant – Iowa State University – Muscle Biology. Supervisors – Steven Lonergan and Joshua Selsby Emily Arkfeld MS. Fall 2013 Meat Science Advisor PhD Student – University of Illinois Mark Anderson Graduate Student Committees at Iowa State University Student Emily Helman Degree M.S.- Summer 2001 Major/Advisor Muscle Biology/Steven Lonergan Monica Foote M.S. - Summer 2001 Nutrition/Don Beitz Jill Sherwood M.S. - Fall 2001 Interdepartmental Genetics/Max Rothschild Stephanie Seiler Lex M.S. - Spring 2002 Biochemistry/Richard Robson Cheryl (Warrick) Dikeman Morris Jason Ellis M.S. - Fall 2002 Nutrition/Allen Trenkle M.S. - Spring 2003 Meat Science/Joe Sebranek Maareen Custodio M.S. Summer 2004 Nutrition/Wendy Powers Trevor Lutz Ph.D. - Fall 2003 Nutrition/Tim Stahly Kasey Maddock Ph.D. Summer 2005 Meat Science/Steven Lonergan Kyung Yuk Ko Ph.D. Summer 2007 Meat Science/Dong Ahn Kristen M. Carnagey Ph.D Summer 2006 Nutrition/Don Beitz Hehsam A. Ismail Ph.D. Summer 2007 Meat Science/Dong Ahn Ryan Husak M.S. Spring 2007 Meat Science/Joe Sebranek Xingqui Lou Ph.D. Expected Fall 2008 Meat Science/Steven Lonergan Cory Wagner M.S. Summer 2007 Meat Science/Steven Lonergan Ye Cheng Ph.D. Fall 2010 Animal Breeding and Genetics/James Reecy Rachel Smith M.S. Summer 2009 Meat Science/Steven Lonergan Elisabeth Huff Lonergan 38 Judson Kyle Grubbs Ph.D. Fall 2012 Meat Science/Steven Lonergan Katrin Hollinger PhD. Spring 2014 Physiology/Josh Selsby Sarah Pierce PhD Summer 2014 Animal Nutrition/Nick Gabler Olivia Genther PhD Summer 2014 Animal Nutrition/Stephanie Hansen Charlwit Kulchaiyawat PhD Expected Spring 2015 Food Science/Tong Wang Jason Russell PhD Expected Fall 2016 Animal Nutrition/Stephanie Hansen Congmu Zhang MS Expected Summer 2015 Ag and Biosystems Engineering/Kurt Rosentrater Allyson Arrick MS Expected Fall 2015 Ag and Biosystems Engineering/Kurt Rosentrater Cayla Iske MS Expected Fall 2015 Animal Nutrition/Cheryl Morris Kelsey Carlson MS Expected Spring 2016 Meat Science/Steven Lonergan Graduate Student Committees at International Institutions Student Degree Marianne Nissen Lund PhD –November 29, 2007 Julie Marsh PhD – Biological Sciences - 2005 Institution/Major Professor University of Copenhagen, Demark/Dr. Leif Skibsted, professor of Food Chemistry University of Waikato – New Zealand/ Dr. Nicholas Ling and Dr. Clyde Daly Undergraduate Advising at Iowa State University Currently advise 30 undergraduates in the Department of Animal Science. Elisabeth Huff Lonergan 39 Research (75% of Appointment – 1998-2011, 50% of Appointment 2012-2015) Grants Directed or Co-Directed (names in bold identify the Principal Investigator) Grants Received Prior to Appointment at Iowa State University Investigators Title 1. S. M. Lonergan Characterization of variations in E. Huff-Lonergan calpastatin expression and postmortem changes in beef. Amount $11,200 Source Alabama Cattlemen’s Association Year 96-97 2. W.B. Mikel, W.R. Jones, L.S. Mikel, E. HuffLonergan, S.M. Lonergan, and D.R. Mulvaney Various methods of value added merchandising for low quality cuts of beef in the retail market. $11,298 Alabama Cattlemen’s Association 96-97 3. E. Huff-Lonergan S.P. Schmidt Instructional technology equipment grant. $23,700 Auburn University 1996 4. E. Huff-Lonergan S. M. Lonergan W. R. Jones Color stability and tenderness properties of processed and fresh product from beef from vitamin E fed cattle. $11,000 Alabama Cattlemen’s Association 97-98 5. E. Huff-Lonergan S. M. Lonergan Biochemical characterization of the proteolytic enzyme, calpain and its endogenous inhibitor, calpastatin. $52,264 Auburn University Competitive Biogrants Program 96-98 6. E. Huff Lonergan S. M. Lonergan D. Kuhlers S. Jungst Identification of biological mechanisms influencing pork quality and efficient production of pork in the absence of the halothane gene. $24,500 97-98 7. S. M. Lonergan E. Huff-Lonergan Identification of factors contributing to variation in tenderness of beef top loin steaks from Brangus cattle. $13,500 National Pork Producer’s Council Competitive Research Program Alabama Cattlemen’s Association 8. E. Huff-Lonergan S. M. Lonergan Role of endogenous calpain/calpastatin proteolytic system in muscle maintenance of geriatric and young beagles. $16,845 The Iams Company 1998 Total $ funded at Auburn University (6/95 – 9/98) $164,307 Total $ of proposals funded as PI at Auburn University (6/95 – 9/98) $128,309 97-98 Elisabeth Huff Lonergan 40 Grants Received While on Faculty at Iowa State University Investigators Title 9. E. Huff-Lonergan The effect of dietary treatments S.M. Lonergan on the endogenous calpain/calpastatin proteolytic system in canine skeletal muscle. 10. E. Huff-Lonergan J. Page Development of a chilling module for pork carcasses. 11. S. M. Lonergan, E. Huff-Lonergan 12. Amount Source $20,835 The Iams Company Year 1999 $4,005 National Pork Producer’s Council 1999 Characterization of muscle glycogen storage and utilization: Influence on pork quality. $10,600 National Pork Producers Council Competitive Grants Program 99-00 D. Beitz, A. Trenkle, E. Huff-Lonergan, F.C. Parrish, and R. Horst Use of vitamin D3 and its metabolites to improve beef tenderness. $20,000 National Cattlemen’s Beef Association 99-00 13. E. Huff-Lonergan Efficacy of AquAir treatment in maintaining the shelf-life of fresh meat. 14. E. Huff-Lonergan S.M. Lonergan Influence of early postmortem factors on water-holding capacity and tenderness of fresh pork. $19,332 National Pork Producers’ Council 00-01 15. S. M. Lonergan E. Huff-Lonergan J.G. Sebranek Improving shelf life and quality traits of minimally processed pork loins. $24,795 Iowa Pork Producers Association 00-01 16. E. Huff-Lonergan S. M. Lonergan *Influence of protein oxidation on mechanisms governing meat tenderness. *Funded on first submission $112,000 USDA, CSREES National Research Initiative Competitive Grants Program 17. D. Beitz,. A. Trenkle, E. Huff-Lonergan, F.C. Parrish, and R. Horst Use of 25-hydroxyvitalmin D to improve tenderness of beef. $25,000 Iowa Cattlemens’ Beef Association 01-02 18. E. Huff-Lonergan S. M. Lonergan E. P. Berg Novel Approach to Controlling Water-Holding Capacity and pH Decline in Pork: The Role of $28,832 National Pork Board 01-02 $7,323 Applied Humidity Technologies and Boldt Marketing Group 2000 00-04 Dietary Lipoic Acid in Calcium Regulation and Glycogen Storage. Elisabeth Huff Lonergan 41 19. J. Briggs E. Huff-Lonergan Optimizing chilling using numerical modeling. $25,860 National Pork Board 01-02 20. S. M. Lonergan E. Huff-Lonergan C. Schultz-Kaster Influence of harvest processes on loin and ham quality $18,162 National Pork Board 01-02 21. E. Huff-Lonergan S. M. Lonergan Impact of proteolysis on pH decline and water holding capacity of fresh pork. $31,600 National Pork Board 02-03 22. E. Huff-Lonergan S. M. Lonergan Role of muscle cytoskeletal and intermediate filament proteins in the development of soft and exudative pork. $24,500 National Pork Board 02-03 23. S. M. Lonergan E. Huff-Lonergan C. Schultz-Kaster Optimization of commercial harvest processes that affect pork water holding capacity and color. $30,460 National Pork Board 02-03 24. J. Sebranek, D. Marple, S.M. Lonergan, K. Prusa, D. Ahn, E. HuffLonergan D. Buege, M. Doumit Pork Muscle Profiling Study2002. $235,150 National Pork Board 02-03 25. G. Marquis, D. Acker, L Butler, E. Huff-Lonergan H Jensen, E. Colecraft, M Reddy, O Sakyi-Dawson, A Lartey, E Canacoo, B Ahunu Eliminating the contsraints on the incorporation of animal source proteins in the diets of Ghanaian children; a planning grant. $49,997 Global Livestock Collaborative Research Support Program. 2003 26. E. Huff-Lonergan S. M. Lonergan *Influence of protein oxidation on mechanisms governing the calpain system and meat tenderness. 03-07 27. W. Powers, E. HuffLonergan Comparison of growth and carcass traits for pigs fed Bt (Bt11) and conventional corn $170,000 USDA, CSREES National Research Initiative Competitive Grants Program $33,534 Sygenta 28 E. Huff-Lonergan Travel to attend Cold Spring Harbor Workshop on Proteomics 29 GS Marquis, E Colecraft, L Butler, H Jensen, M Reddy, E HuffLonergan,O SakyeDawson, A Lartey, B. Ahunu, E Canacoo ENAM in Africa: Enhancing child nutrition through animal source food management *Funded on first submission $1,000 Center for Integrated Animal Genomics (CIAG) – ISU $747,985 USAID/CRSP 03-04 2004 04-07 Elisabeth Huff Lonergan 42 30 D. Beitz, E. HuffLonergan, S. Lonergan, A. Trenkle Novel strategies to improve tenderness of underutilized round muscle from cows $45,552 National Cattlemen’s Beef Association $15,500 Special Research Initiation Grant, Iowa State University $27,650 National Cattlemen’s Beef Association 31 E. Huff-Lonergan and James Reecy Identification of differentially expressed proteins in a model of enhanced skeletal muscle growth 32 D. Beitz, E. HuffLonergan, S. Lonergan, A. Trenkle Novel strategies to improve tenderness of underutilized round muscle from cows – Addendum 33 J. Reecy and E. HuffLonergan Molecular mechanisms controlling cardiac and skeletal muscle growth and development 34 M.F. Rothschild, S. Lonergan, E. HuffLonergan, J. Dekkers Development and characterization of the ninth generation of a pig resource population for fine mapping of quantitative trait loci 35 S. M. Lonergan, E. Huff-Lonergan, M.F. Rothshchild, and A. Sosnicki Continued selection for rapid growth: Implications for pork quality $90,412 National Pork Board 2005 36 J.F. Baker and E. HuffLonergan Novel technologies in muscle biology/fresh meat research: A symposium $10,000 USDA/NRI Program 42 Animal Growth and Nutrient Utilization 2005 37 J. Reecy, E. HuffLonergan, and J. Dekkers Identification of Modifier Genes of Myostatin Affecting Growth, Body Composition, and Gene Expression. $398,254 USDA/NRI Program 43.0 Animal Genome 06-08 38 N. M. Ellinwood, G.J. McLellen, K.L. Kline, and E. Huff-Lonergan Support for establishment and characterization of large animals of human ocular, cranio-facial, neurological and muscle diseases. $10,000 Center for Integrated Animal Genomics 06-07 39 E. Huff-Lonergan, J. Reecy, S.M. Lonergan, N.M. Ellinwood Acquisition of high-throughput 2-D gel electrophoresis equipment $8,376 2006 40 E. Huff-Lonergan and S. M. Lonergan Round muscle profiling: Management of tenderness and sensory improvement of specific muscles with aging. $41,570 Center for Integrated Animal Genomics National Cattlemen’s Beef Association $8,480 Center for Integrated Animal Genomics $10,000 Center for Integrated Animal Genomics 04-05 2005 2005 04-05 04-05 06-07 Elisabeth Huff Lonergan 43 41 D.C. Beitz, E. HuffLonergan, S.M. Lonergan, A. Trenkle, and J. Russell A novel approach to improving tenderness of underutilized muscles from the round from pasture-fed beef steers $86,018 National Cattlemen’s Beef Association 06-07 42 E. Huff-Lonergan and S.M. Lonergan *Protein Oxidation and Nitration: Novel mechanisms governing meat quality $320,474 USDA, CSREES National Research Initiative Integrated Program – Program 71 06-11 *Funded on first submission – ranked in Highest Priority Category – received full funding 43 GS Marquis, E Colecraft, L Butler, H Jensen, M Reddy, E HuffLonergan,O SakyeDawson, A Lartey, B. Ahunu, E Canacoo Daycare Center Feeding Programs And Their Influence On Dietary Quality Among Poor Ghanaian Children $179,785 Global Livestock CRSP, UC Davis (US-AID Funds) 06-08 44 S.M. Lonergan, E. Huff-Lonergan, C.R. Calkins Identification of protein markers for beef tenderness in enhanced beef $20,091 National Cattlemen’s Beef Assn. 07-08 45 E. Huff-Lonergan and S.M. Lonergan National Cattlemen’s Beef Assn. 07-08 46 N.M. Ellinwood, E. Huff-Lonergan, and D.L Dobbs $34,389 Round muscle profiling: Novel markers to indicate tenderness potential $28,500 Structural and functional studies of Naglu: Development of Pharmacopin based therapy for MPS IIIB Center for Integrated Animal Genomics 07-08 47 J.C.M.Dekkers, A.K. Butters-Johnson, M. Spurlock, C.K. Tuggle, S. Lonergan, E. HuffLonergan, L. Anderson Identification of biological factors responsible for differences in feed efficiency between selection lines for residual feed intake National Pork Board 07-08 48 S.M. Lonergan, E. Huff-Lonergan, and J. Sebranek Influence of High Oxygen $60,274 Packaging Systems and Enhancement on Tenderness Development and Appearance of Beef Cuts from the Round Iowa Beef Industry Council. 08-09 49 E. Huff-Lonergan and S.M. Lonergan Development of a white paper: Round muscle profiling and tenderness National Cattlemen’s Beef Assn. 08-09 $101,949 $4,500 Elisabeth Huff Lonergan 44 markers in beef 50 E. Huff-Lonergan and S.M. Lonergan *Protein Oxidation and Nitration: Novel mechanisms governing meat quality (supplemental award to the original grant) $10,000 USDA, CSREES National Research Initiative Integrated Program – Program 71 06-09 51 E.Huff-Lonergan, S.M. Lonergan, and Y.H. Kim Improving the Quality of the Beef Round: What is the role of Electrical Stimulation? $50,833 09-10 52 E. Huff-Lonergan,S.M. Lonergan, J. Sebranek, Y.H. Kim, and D. Visser $66,956 53. S.M. Lonergan, E. Huff-Lonergan and 5 PIs Improving Quality of Beef Round Muscles Packaged in High-Oxygen Modified Atmosphere through Early Postmortem Calcium Lactate Enhancement Upgrade of high performance chemiluminescence and fluorescence imaging system National Cattlemen’s Beef Association National Cattlemen’s Beef Association 09-10 54 S.M. Lonergan, E. Huff-Lonergan $14, 341 55 E. Huff Lonergan Method to improve meat tenderness with added calpain proteinases. Development of a Pet Food Processing and Nutrition course in the College of Agriculture and Life Sciences Center for Integrated Animal Genomics Johnsonville, LLC College of Agriculture and Life Sciences – Iowa State University 10-11 56 Project Director Dr. John F. Patience, Iowa State University Collaborating Investigators (CoPDs) Dr. Lloyd Anderson, Dr. Roger Campbell, Pork Cooperative Research Center (Australia) Dr. Jack Dekkers, Dr. Joel DeRouchey, Kansas State University Dr. Nicholas Gabler, Dr. Helene Gilbert, Institut National de la Recherche Agronomique, (France) Dr. Anna Johnson, Dr. Brian Kerr, National Laboratory for Agriculture and the Environment, U.S.D.A. Dr. Peng Liu, Iowa State University Dr. Elisabeth Huff Lonergan, 10% Dr. Steven Lonergan, , Dr. John Mabry, Dr. Dan Nettleton, Dr. Max Rothschild, Improving nutrient utilization and feed efficiency through research and extension to enhance pig industry sustainability and competitiveness $8,000 $7,500 $5,000,000 AFRI 09-10 09-10 11-15 Elisabeth Huff Lonergan 45 Dr. Raymond R.R. Rowland, Kansas State University Dr. Mike Tokach, Kansas State University Dr. Chris Tuggle, Iowa State University Dr. Andrew van Kessel, University of Saskatchewan (Canada) Dr. Tom Weber, Laboratory for Agriculture & the Environment, U.S.D.A. 57 E. Huff-Lonergan S.M. Lonergan Novel markers to identify tenderness in early postmortem beef $27,276 National Cattlemen’s Beef Assn. 10-11 58 E. Huff-Lonergan (40%) S. M. Lonergan S.M. Cruzen Molecular diversity of calpastatin: Implications for muscle protein turnover and beef quality. $29, 937 ISU Burroughs Fund 59 S.M. Lonergan E. Huff-Lonergan – 25% J.G. Sebranek K.J. Prusa Influence of commercial deep chilling processes on early postmortem events in muscle that influence ultimate fresh pork tenderness and processing quality 60 S.M. Lonergan E. Huff-Lonergan Determination of protein markers for beef tenderness in lean beef: Critical information towards improving beef cattle management and enterprise profitability $24,995 ISU Burroughs Fund 2012 61 S.M.Lonergan E. Lonergan Determination of protein markers for beef tenderness in U. S. Select Beef $48,394 Iowa Beef Industry Council 2014 11-12 $135, 173 National Pork Board Total $ of proposals funded as PI or Co-PI during her career (1996-2014) 11-13 $8,758,046 Elisabeth Huff Lonergan 46 Visiting Scientists Co-Hosted Scientist Institution Year Time Frame Dr. Giuseppe Bee Swiss Federal Research Station for Animal Production 2003 2005 7 months 1 month Javier Peinado del Pino Imasde (private agricultural research consulting firm from Spain) 2003 3 weeks Dr. Jin Hyoung Kim National Livestock Research Institute in the Republic of Korea. 2003-2004 10 months Dr. René Lametsch KVL University, Fall 2006 Copenhagen, Denmark 3 months Javier Viguera Imasde (private agricultural research consulting firm from Spain) Summer 2007 3 weeks Dr. Pedro Veiga Paulino Universidade Federal de Vicosa – Vicosa, Brazil 2011 1 year Leonardo Oliveira PhD Student Federal University of Goiás - Brazil 2014-2015 1 year Reviewing Editing and Professional Service Activities in Research Meat Science (Elsevier Press – and official journal of the American Meat Science Association) – Associate Editor. Handle approximately 300 articles per year. 2008-2015 Journal of Muscle Foods - Editorial Board Member: 2000-2011. Journal of Animal Science (the official Journal of the American Society of Animal Science) - Editorial Board Member: 1997-2000, Ad-Hoc Reviewer: 2001-2007 Editorial Board Member: 2008-2010. AdHoc Reviewer: 2011-2015. Fleischwirtschaft – Editorial Board Member – 2008-2015 National Research Initiative Competitive Grants Program (USDA/CSREES/NRICGP) Value Added Products Research - Ad Hoc Reviewer-1997, 1998, 2001, 2003, 2004, 2006 Panel Member – Panel 71.1. Improving Food Quality and Value – 2005, 2007 Journal of Food Science - Ad Hoc Reviewer - 2001-2015 Food Science and Technology – Ad Hoc Reviewer – 2000, 2001 Institute of Food Technologists. Muscle Foods Division Committee Member-at-Large. 1999-2001 Journal of Agricultural and Food Chemistry – Ad Hoc Reviewer – 2003-2015. Elisabeth Huff Lonergan 47 Extension/Professional Practice Professional Outreach and Service – Specific Activities Iowa Meat Processors Annual Convention Processed Meats Contest – Judge – 1999, 2000, 2006. World Pork Expo – National Pork Producers Council – Member of a national committee to plan and implement and staff an interactive display showcasing Pork 101. 1999 and 2000. Targeted Pork Quality Research Consortium Planning Committee Member – National Pork Producers’ Council. – Invited member, invited Nov. 1999, member 1999 - 2000. Pork Industry Meeting on GMO’s – Invited meat science expert. Nov. 22, 1999. National Pork Producers Council Pork Quality Solutions Committee – Invited member, invited 1999, member 1999 - 2003. Unified Research Meeting, National Pork Producers Council. – Member of the Meat Quality Solutions Team – purpose of the meeting was to review research proposals and set programs for 2001. December 4-5, 2000. Unified Research Meeting, National Pork Producers Council. – Member of the Meat Quality Solutions Team – purpose of the meeting was to review research proposals and set programs for 2002. December 4-6, 2001. Pork 101- National Pork Producers Association – Instructor, Pork Quality, 1999-2004. Benchmarking Value in the Pork Chain – American Meat Science Association – Nov. 20 2003. Pork Quality and Safety Summit, National Pork Board. August 2004 American Society of Animal Science – Chair of the Muscle Biology/Meat Science Committee - 2005 American Society of Animal Science – Organized a Muscle Biology/Meat Science Symposium titled “Novel Technologies in Muscle Biology/Fresh Meat Research” - this symposium was attended by approximately 250 people. The presentations of 3 of the speakers were also written as papers and submitted to the Journal of Animal Science for publication in 2006. Titles of the talks and the names of the speakers included: Use of transgenic models to understand skeletal muscle function - Dr. Melissa Spencer - UCLA Contribution of microarray data in understanding muscle development - Dr. Jim Reecy – Iowa State University Use of molecular technologies in understanding skeletal adipose tissue development and maintaince -Dr. Mike Spurlock – Purdue University Role of proteomics in meat science research - Dr. Rene Lamtesch - Danish Institute of Agricultural Sciences, Tjele, Denmark. 4-H “Beef Blast” – March 21, 2007. Provided a demonstration on tenderness differences of different beef cuts to 74 4-H members ranging in age from 12-17 years. FASEB Summer Conference – Biology of Calpains in Health and Disease. Chair of the session titled “Calpains in Animal Science” –July 14-19, 2007. National Cattlemen’s Media Training – conducted by Daren Williams, Executive Director NCBA Communications. Iowa Cattlemen’s Beef Association Headquarters-Ames, IA. Jan. 5, 2009. Elisabeth Huff Lonergan 48 FASEB Summer Conference – Biology of Calpains in Health and Disease. Chair of the session titled “Calpains in Muscle” –July 21-26, 2013. Outreach on Campus Sigma Alpha Sorority. September 13, 1999. Careers in the Meat Industry. Invited talk (Approx. 40 students in attendance). ISU Block and Bridle – Meats Interest Group. Oct 1999. Pork Quality. Invited talk (Approx. 10 students in attendance) Iowa State University/ConAgra Industry Shortcourses Lecturer on Meat Color Level 1 – 3 lectures in 2001, 2 lectures in 2002, 2 lectures in 2003, 2 lectures 2004., 1 lecture in 2005 Iowa State University/ConAgra Industry Shortcourses Lecturer on Meat Color Level 2 – 2 lectures in 2001, 2 lectures in 2002, 2 lectures in 2003, 2 lectures 2004. ISU Block and Bridle – Joint meeting of Swine Interest Group and Meats Interest Group. Oct 2000, 2001. Future Trends in Pork Quality – Realities for the producer and packer. Invited talk (Approx. 25 students in attendance). International Activities Spanish Sausage and Processed Meats Shortcourse in July 2000. There were approximately 75 attendees from South and Central America in attendance. Member of Pork Trade Mission to China - March 8-16, 2002. Trip organizer - Mark Fisher of the Iowa Department of Economic Development. Team included members of the Iowa Department of Economic Development, the Iowa Pork Producer's Council (President of the Council and Executive Vice President), Iowa Farm Bureau, and the National Pork Board. Meat Export Federation. Approximately 20 lectures on pork quality to different international groups over the past 3 years for a total of about 300 international visitors reached. USAID/CRSP (see grants # 26 and 29 and 43 )Project leader is in Food Science (Grace Marquis) and other faculty involved are from Agricultural Economics (Helen Jensen), Food Science (Manju Reddy), Global Agriculture (Lorna Butler) and faculty members from the University of Ghana. Traveled to Ghana to work on the project in July 2006 International Congress of Meat Science and Technology. Chaired a session on fresh meat biochemistry August 5-10, 2007 in Beijing, China. China Agricultural University – Invited to visit with the faculty of Animal Science about research and tour their facilities. August 8, 2007. Food Science Department of the University of Copenhagen, Denmark. - Member of the assessment committee of a PhD. student, Marianne Nissen Lund. Final exam is scheduled for November 29, 2007 in Copenhagen. Nanjing University, Nanjing, China – Invited to give two lectures to the Animal Science faculty and staff and to explore research opportunities. September 2011 Elisabeth Huff Lonergan 49 Diversity Activities at Iowa State University Provost’s Office – Faculty Fellow – ADVANCE – August 2012 – present (25% Administrative appointment). Responsible for coordinating the activities related to the advancement and faculty satisfaction of all faculty on campus with a particular emphasis on women and underrepresented groups on campus. ADVANCE Professor for the Department of Animal Science-August 2008-December 2010 (NSF funded initiative) – responsible for facilitating the identification and implementation of new policies and practices to enhance departmental communication and workplace satisfaction and to work with College and University level administrators/researchers in evaluating the effectiveness of the changes. PWISE (Program for Women in Science and Engineering) Mentor- Summer 2005 and 2006 Mentor for Abbey (Avery) Canon in 2005 and Kathy Mou in 2006. Institutional Service Institutional Service at Auburn University York Lecture Committee - member Animal and Dairy Sciences Dept. Building Space Utilization Committee -member Animal and Dairy Sciences Dept. Semester Transition Muscle Biology/Meat Science Curriculum Committee - member Animal and Dairy Sciences Dept. Budget Committee - member Auburn University Traffic Appeals Board - member Animal and Dairy Sciences Dept. Visibility and Outreach Effectiveness Committee-member 1995 1996-97 1997 1997 1997-98 1998 Institutional Service at Iowa State University Departmental Level Member Chuckwagon Breakfast committee, 1999-2000, 2004, 2006-2009, 2011-2014 Member of the Animal Science Department Strategic Plan Committee, 2000. The purpose of this committee was to develop and write the new strategic plan for the department Member of the Search Committee for the Animal Science Department Interim Chair, 2001 Faculty supervisor for a secretary for three faculty members in the department, 2002-present Member of the Animal Science Department Seminar Committee, 2002-2005 Chair of the Animal Science Department Graduation Breakfast Committee, 2003-present Member of the Search Committee for the Animal Science Department Chair, 2003 Chair of the Animal Science Seminar Committee, 2003-2004 Faculty Advisor for the Meat Science Graduate Student Organization, 2001-current Chair of the Search Committee for the Food/Meat Safety position in Animal Science. 2005 Animal Science Department Undergraduate Curriculum Committee – 2006, 2007 Member of the Search Committee for a faculty member in Beef Feedlot Nutrition – 2008 Chair of the Search Committee for a lecturer (faculty) in Companion Animal Science. – 2008 Chair of the Peer Review of Teaching development committee – 2010-2011 Mentor to 3 faculty – 2 Associate Professors in Animal Science (Dr. Anna Butters Johnson and Dr. Lance Baumgard) and 1 Assistant Professor in Animal Science (Dr. Stephanie Hansen) Mentor to a Preparing Future Faculty student – Katrin Hollinger (2011-present) Member of the Search Committee for the Animal Science Department Chair, 2013-14 Mentor to a Graduate College Emerging Leader – Trey Kellner (2015) Elisabeth Huff Lonergan 50 College Level Member of the Iowa State University College of Agriculture Professional Development Committee, 2000-2002. The role of this committee was to evaluate travel grants for activities related to outreach and teaching and to aid in defining professional development activities/conferences for the college. Faculty Advisor for Sigma Alpha, Delta Chapter, 2003-2006. Sigma Alpha is a professional agricultural sorority for undergraduate women in agriculture. The Delta chapter is the Iowa State University chapter of this national sorority. Member of the College of Agriculture and Life Sciences Promotion and Tenure Committee – 2010, 2011 Chair - College of Agriculture and Life Sciences Promotion and Tenure Committee - 2012 University Level Member of the Iowa State University Faculty Senate Committee on Appeals, 2000 – 2001. The role of this committee was to hear grievance cases from faculty and students. Member of the Iowa State University Nutritional Sciences Council Current Issues in Nutrition Conference committee, 2001-2005. The role of this committee was to plan and host the annual nationwide satellite conference for dieticians and health professionals. Co-Chair of the Iowa State University Nutritional Sciences Council Current Issues in Nutrition Conference committee, 2004-2005. The role of this committee was to plan and host the annual nationwide satellite conference for dieticians and health professionals. Member of the Iowa State University Nutritional Sciences Council Research Committee, 20012005. The role of this committee is to review grant proposals and recommend funding for seed grants administered by the Nutritional Sciences Council. Member of the Iowa State University Bioethics Committee, 2001-2007. committee is to assist in planning university wide bioethics programming. The charge of this Member of the Iowa State University Graduate Council, 2006-2009. The Graduate Council serves as the executive committee of the graduate faculty. The Graduate Council represents the Graduate Faculty, serves in an advisory capacity to the Dean of the Graduate College, approves new graduate programs, and establishes educational policies that govern graduate education throughout the College. The Council is elected and meets at least monthly during the academic year. ADVANCE Professor 2008-2010 – embedded in the Department of Animal Science Elisabeth Huff Lonergan