Product datasheet
Anti-ITGB6 antibody [EPR15740] ab187155
4 Images
Overview
Product name
Anti-ITGB6 antibody [EPR15740]
Description
Rabbit monoclonal [EPR15740] to ITGB6
Tested applications
WB, IP
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within
Human ITGB6 aa 100-200. The exact sequence is proprietary.
Database link: P18564
Positive control
Human fetal skin and fetal kidney lysate. 293 and A549 cell lysate. C6, Raw264.7, PC12 and
NIH3T3 cell lysate.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Purity
Protein A purified
Clonality
Monoclonal
Clone number
EPR15740
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab187155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 86 kDa.
IP
1/30 - 1/50.
Target
Function
Integrin alpha-V/beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence RG-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis
promotes carcinoma cell invasion.
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 VWFA domain.
Cellular localization
Membrane.
Anti-ITGB6 antibody [EPR15740] images
All lanes : Anti-ITGB6 antibody [EPR15740]
(ab187155) at 1/10000 dilution
Lane 1 : Human fetal skin lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the nonreduced form of IgG at 1/1000 dilution
Western blot - Anti-ITGB6 [EPR15740] antibody
(ab187155)
Predicted band size : 86 kDa
Blocking/ Dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-ITGB6 antibody [EPR15740]
(ab187155) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase
conjugated at 1/1000 dilution
Western blot - Anti-ITGB6 [EPR15740] antibody
(ab187155)
Predicted band size : 86 kDa
Blocking/ Dilution buffer: 5% NFDM /TBST.
All lanes : Anti-ITGB6 antibody [EPR15740]
(ab187155) at 1/10000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Western blot - Anti-ITGB6 [EPR15740] antibody
Goat Anti-Rabbit IgG, (H+L), Peroxidase
(ab187155)
conjugated at 1/1000 dilution
Predicted band size : 86 kDa
Blocking/ Dilution buffer: 5% NFDM /TBST.
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Lysate from 293 cells was
immunoprecipitated with ab187155 at a 1/50
dilution. For the subsequent blot, ab187155
used at a 1/10000 dilution with Goat AntiRabbit IgG, (H+L), Peroxidase conjugated at
a 1/1000 dilution for the secondary. Blocking/
Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-ITGB6 [EPR15740]
antibody (ab187155)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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