Product datasheet
Anti-NFIB / NF1B2 antibody [NFI5I299] ab51352
2 Abreviews 1 Image
Overview
Product name
Anti-NFIB / NF1B2 antibody [NFI5I299]
Description
Mouse monoclonal [NFI5I299] to NFIB / NF1B2
Tested applications
Dot Blot, IP, WB
Species reactivity
Reacts with: Human
Immunogen
Recombinant fragment (Human)
Positive control
HeLa cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Protein G purified
Purification notes
Purified using protein G column chromatography from culture supernatant of hybridoma cultured
in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered
through a 0.22 micrometer membrane.
Clonality
Monoclonal
Clone number
NFI5I299
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab51352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
Dot Blot
Use at an assay dependent dilution.
IP
Use at 20 µg/mg of lysate.
WB
1/100. Detects a band of approximately 49 kDa (predicted molecular weight: 47
kDa).
1
Target
Function
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral
and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are
individually capable of activating transcription and replication.
Sequence similarities
Belongs to the CTF/NF-I family.
Contains 1 CTF/NF-I DNA-binding domain.
Cellular localization
Nucleus.
Anti-NFIB / NF1B2 antibody [NFI5I299] images
ab51252 used at 10µg/500µg RIPA lysate of
HeLa cells. Western blot labelled with
ab51352 at 1/100 dilution followed by
TrueBlot anti Mouse IgG at 1/1000 dilution.
Developed using the ECL technique.
Immunoprecipitation - NFIB / NF1B2 antibody
[NFI5I299] (ab51352)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
2