Product datasheet
Anti-beta Crystallin A3 antibody ab151786
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Overview
Product name
Anti-beta Crystallin A3 antibody
Description
Rabbit polyclonal to beta Crystallin A3
Tested applications
WB, ICC/IF
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Dog, Monkey
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-215 of Human beta
Crystallin A3 (UniProt: P05813).
Positive control
293T, A431, H1299, HeLa, Molt4 and Raji whole cell lysates, HeLa cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab151786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 25 kDa.
ICC/IF
1/100 - 1/1000.
Target
Function
Crystallins are the dominant structural components of the vertebrate eye lens.
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Involvement in disease
Defects in CRYBA1 are the cause of cataract congenital zonular with sutural opacities (CCZS)
[MIM:600881]. A form of zonular cataract. Zonular or lamellar cataracts are concentric opacities,
broad or narrow, usually consisting of powdery white dots affecting one lamella or zonule of an
otherwise clear lens.
Sequence similarities
Belongs to the beta/gamma-crystallin family.
Contains 4 beta/gamma crystallin 'Greek key' domains.
Domain
Has a two-domain beta-structure, folded into four very similar Greek key motifs.
Post-translational
modifications
Specific cleavages in the N-terminal arm occur during lens maturation and give rise to several
truncated forms. Cleavages do not seem to have adverse effects on solubility.
S-methylation and glutathionylation occur in normal young lenses and do not seem to be
detrimental.
Isoform A1 initiator methionine is removed. The new N-terminal amino acid is then N-acetylated.
Anti-beta Crystallin A3 antibody images
Anti-beta Crystallin A3 antibody (ab151786)
at 1/1000 dilution + Molt4 whole cell lysate at
30 µg
Predicted band size : 25 kDa
Western blot - Anti-beta Crystallin A3 antibody
(ab151786)
Immunofluorescent analysis of methanol-fixed
HeLa cells labeling beta Crystallin A3 with
ab151786 at 1/200. Lower panel co-stained
with Hoechst 33342.
Immunocytochemistry/ Immunofluorescence Anti-beta Crystallin A3 antibody (ab151786)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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