Product datasheet
Anti-alpha B Crystallin (phospho S45) antibody
ab62254
1 Image
Overview
Product name
Anti-alpha B Crystallin (phospho S45) antibody
Description
Rabbit polyclonal to alpha B Crystallin (phospho S45)
Specificity
ab62254 detects endogenous levels of alpha B Crystallin only when phosphorylated at serine
45.
Tested applications
ELISA, IHC-P
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human alpha B Crystallin around the phosphorylation
site of serine 45 (S-L-SP-P-F).
Positive control
Human heart tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Purity
Immunogen affinity purified
Purification notes
ab62254 was affinity-purified from rabbit antiserum by affinity-chromatography using epitopespecific phosphopeptide. The antibody against non-phosphopeptide was removed by
chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab62254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
ELISA
Abreviews
Notes
1/10000.
1
Application
Abreviews
IHC-P
Notes
1/50 - 1/100.
Target
Function
May contribute to the transparency and refractive index of the lens.
Tissue specificity
Lens as well as other tissues.
Involvement in disease
Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB)
[MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb
muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy,
and cataract in a subset of patients.
Sequence similarities
Belongs to the small heat shock protein (HSP20) family.
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear
structures known as SC35 speckles or nuclear splicing speckles.
Anti-alpha B Crystallin (phospho S45) antibody images
ab62254 at 1/50 - 1/100 dilution staining
alpha B Crystallin in Human heart by
Immunohistochemistry, Paraffin-embedded
tissue, in the absence or presence of the
immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - alpha B Crystallin
(phospho S45) antibody (ab62254)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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