Product datasheet
Anti-Alpha SNAP antibody [EPR7372] ab133673
4 Images
Overview
Product name
Anti-Alpha SNAP antibody [EPR7372]
Description
Rabbit monoclonal [EPR7372] to Alpha SNAP
Tested applications
WB, IHC-P, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to a region within Human Alpha SNAP. (P54920)
Positive control
HeLa cell lysate, Molt4 cell lysate, HepG2 cells and cell lysate, Human kidney tissue.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
KD = 9.30 x 10 -11 M
Learn more about KD
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR7372
Isotype
IgG
Applications
1
Our Abpromise guarantee covers the use of ab133673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular
weight: 33 kDa).
IHC-P
1/50 - 1/100. Perform antigen retrieval before commencing with IHC staining
protocol
ICC/IF
Application notes
1/100 - 1/250.
Is unsuitable for Flow Cyt or IP.
Target
Function
Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.
Sequence similarities
Belongs to the SNAP family.
Cellular localization
Membrane.
Anti-Alpha SNAP antibody [EPR7372] images
All lanes : Anti-Alpha SNAP antibody
[EPR7372] (ab133673) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Molt4 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP conjugated antibody at
Western blot - Anti-Alpha SNAP antibody
1/2000 dilution
[EPR7372] (ab133673)
Predicted band size : 33 kDa
2
Immunohistochemical analysis of paraffin
embedded Human kidney tissue labelling
Alpha SNAP with ab133673 at 1/50.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Alpha SNAP
antibody [EPR7372] (ab133673)
Immunofluorescent staining of HepG2 cells
labelling Alpha SNAP with ab133673 at
1/100.
Immunocytochemistry/ Immunofluorescence Anti-Alpha SNAP antibody [EPR7372] (ab133673)
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Other-Anti-Alpha SNAP antibody [EPR7372]
(ab133673)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
3
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
4