Product datasheet
Anti-Kir7.1 antibody - N-terminal ab170631
2 Images
Overview
Product name
Anti-Kir7.1 antibody - N-terminal
Description
Rabbit polyclonal to Kir7.1 - N-terminal
Tested applications
IHC-P, WB
Species reactivity
Reacts with: Human
Predicted to work with: Guinea pig
Immunogen
Synthetic peptide within Human Kir7.1 aa 74-103 (N terminal) conjugated to Keyhole Limpet
Haemocyanin (KLH). The exact sequence is proprietary.
Database link: O60928
Positive control
Human stomach tissue; MDA-MB435 cell line lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Purity
Immunogen affinity purified
Purification notes
ab170631 is purified through a protein A column, followed by peptide affinity purification.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab170631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6
before commencing with IHC staining protocol.
WB
1/100 - 1/500. Predicted molecular weight: 41 kDa.
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Target
Function
Inward rectifier potassium channels are characterized by a greater tendency to allow potassium
to flow into the cell rather than out of it. Their voltage dependence is regulated by the
concentration of extracellular potassium; as external potassium is raised, the voltage range of
the channel opening shifts to more positive voltages. The inward rectification is mainly due to the
blockage of outward current by internal magnesium. KCNJ13 has a very low single channel
conductance, low sensitivity to block by external barium and cesium, and no dependence of its
inward rectification properties on the internal blocking particle magnesium.
Tissue specificity
Predominantly expressed in small intestine. Expression is also detected in stomach, kidney, and
all central nervous system regions tested with the exception of spinal cord.
Involvement in disease
Defects in KCNJ13 are the cause of snowflake vitreoretinal degeneration (SVD) [MIM:193230].
SVD is a developmental and progressive hereditary eye disorder that affects multiple tissues
within the eye. Diagnostic features of SVD include fibrillar degeneration of the vitreous humor,
early-onset cataract, minute crystalline deposits in the neurosensory retina, and retinal
detachment.
Sequence similarities
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ13 subfamily.
Cellular localization
Membrane.
Anti-Kir7.1 antibody - N-terminal images
Anti-Kir7.1 antibody - N-terminal (ab170631)
at 1/100 dilution + MDA-MB435 cell line
lysate at 35 µg
Predicted band size : 41 kDa
Western blot - Anti-Kir7.1 antibody (ab170631)
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Immunohistochemical analysis of formalinfixed, paraffin-embedded Human stomach
tissue labeling Kir7.1 with ab170631 at 1/10
dilution, followed by peroxidase-conjugated
secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Kir7.1 antibody
(ab170631)
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