Product datasheet Anti-p39 antibody [EPR5075(2)] ab129196 2 Images Overview Product name Anti-p39 antibody [EPR5075(2)] Description Rabbit monoclonal [EPR5075(2)] to p39 Tested applications WB, IP, ICC/IF Species reactivity Reacts with: Mouse, Rat, Human Immunogen A synthetic peptide corresponding to residues in Human p39. Positive control Neuro 2a, HeLa, SH-SY5Y, U-87 MG, and Human oligodendroglioma lysates. General notes This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. Properties Form Liquid Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. Dissociation constant (KD) KD = 5.40 x 10 -12 M Learn more about KD Storage buffer pH: 7.20 Preservative: 0.01% Sodium azide Constituents: 49% PBS, 50% Glycerol, 0.05% BSA Purity Tissue culture supernatant Clonality Monoclonal Clone number EPR5075(2) Isotype IgG Applications 1 Our Abpromise guarantee covers the use of ab129196 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews WB Notes 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa). IP 1/10 - 1/100. ICC/IF 1/250 - 1/500. Application notes Is unsuitable for Flow Cyt or IHC-P. Target Function Activator of CDK5/TPKII. Tissue specificity Brain and neuron specific. Sequence similarities Belongs to the cyclin-dependent kinase 5 activator family. Anti-p39 antibody [EPR5075(2)] images All lanes : Anti-p39 antibody [EPR5075(2)] (ab129196) at 1/1000 dilution Lane 1 : Neuro 2a lysate Lane 2 : HeLa lysate Lane 3 : SH-SY5Y lysate Lane 4 : U-87 MG lysate Lane 5 : Human oligodendroglioma lysate Lysates/proteins at 10 µg per lane. Western blot - Anti-p39 antibody [EPR5075(2)] Secondary (ab129196) Goat anti-Rabbit HRP at 1/2000 dilution Predicted band size : 39 kDa Equilibrium disassociation constant (KD) Learn more about KD Click here to learn more about KD Other-Anti-p39 antibody [EPR5075(2)](ab129196) 2 Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3