Product datasheet
Anti-Nicotinic Acetylcholine Receptor alpha 1 + 3 + 5
antibody [210] ab24719
1 References 1 Image
Overview
Product name
Anti-Nicotinic Acetylcholine Receptor alpha 1 + 3 + 5 antibody [210]
Description
Rat monoclonal [210] to Nicotinic Acetylcholine Receptor alpha 1 + 3 + 5
Specificity
This antibody was generated in rats against bovine and mouse muscle nicotinic acetylcholine
receptors (AChRs). It binds to the main immunogenic region (MIR) on the extracellular surface of
muscle AChR a1 subunit. This antibody binds to the alpha 3 and alpha 5 subunits of human
neuronal AChRs. The antibody binds the alpha 1 subunit weakly when the protein is denatured. It
can detect the alpha 3 and alpha 5 subunit native forms only, and therefore does not detect these
two subunits in immunoblotting.
Tested applications
Flow Cyt, WB, IP, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Chicken, Hamster, Dog, Human, Ferret, Hedgehog, Chinese
Hamster
Does not react with: Xenopus laevis
Immunogen
Full length protein: bovine and mouse muscle nicotinic acetylcholine receptors.
Positive control
Transfected QT6 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None.
Constituents: Ascites.
Purity
Ascites
Clonality
Monoclonal
Clone number
210
Isotype
IgG1
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab24719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1
Application
Abreviews
Flow Cyt
Notes
Use at an assay dependent concentration. PubMed: 18825779ab18407-Rat
monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
1/5000. Predicted molecular weight: 51 kDa.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Target
Relevance
The Nicotinic Acetylcholine Receptors (AChRs) are members of a superfamily of ligand gated
ion channels that mediate fast signal transmission at synapses. These receptors are thought to
be hetero pentamers composed of homologous subunits. The proposed structure for each
subunit is a conserved N terminal extracellular domain followed by three conserved
transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane
domain, and a short C terminal extracellular region. After binding acetylcholine, the Nicotinic
Acetylcholine Receptor alpha responds by an extensive change in conformation that affects all
subunits and leads to opening of an ion conducting channel across the plasma membrane.
Cellular localization
Integral membrane protein.
Anti-Nicotinic Acetylcholine Receptor alpha 1 + 3 + 5 antibody [210] images
Clustered acetylcholine receptors in
transfected QT6 cells, visualised using
ab24719 at a 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence Anti-Nicotinic Acetylcholine Receptor alpha 1 + 3
+ 5 antibody [210] (ab24719)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
2
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
3