Product datasheet
Anti-Muscarinic Acetylcholine Receptor M4 antibody Cytoplasmic domain ab150518
1 Image
Overview
Product name
Anti-Muscarinic Acetylcholine Receptor M4 antibody - Cytoplasmic domain
Description
Rabbit polyclonal to Muscarinic Acetylcholine Receptor M4 - Cytoplasmic domain
Specificity
BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
Tested applications
IHC-P
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Hamster, Cow, Dog, Pig, Gorilla
Immunogen
Synthetic peptide, corresponding to 20 amino acids from the 3rd cytoplasmic domain of Human
Muscarinic Acetylcholine Receptor M4 (NP_000732.2).
Positive control
Human brain, neurons and glia tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C.
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 99% PBS
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab150518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with
citrate buffer pH 6 before commencing with IHC staining protocol.
Target
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Relevance
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors.
The functional diversity of these receptors is defined by the binding of acetylcholine to these
receptors and includes cellular responses such as adenylate cyclase inhibition,
phosphoinositide degeneration, and postassium channel mediation. Muscarinic receptors
influence many effects of acetylcholine in the central and peripheral nervous system. The clinical
implications of this receptor are unknown; however, mouse studies link its function to adenylyl
cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in
many regions of the rat brain, particularly the striatum and hippocampus.
Cellular localization
Cell Membrane
Anti-Muscarinic Acetylcholine Receptor M4 antibody - Cytoplasmic domain images
Immunohistochemical analysis of Formalinfixed, Paraffin-Embedded Human brain,
neurons and glia tissue labeling Muscarinic
Acetylcholine Receptor M4 with ab150518 at
3 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Muscarinic
Acetylcholine Receptor M4 antibody (ab150518)
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