Product datasheet
Anti-Metabotropic Glutamate Receptor 3 antibody
ab10309
1 Image
Overview
Product name
Anti-Metabotropic Glutamate Receptor 3 antibody
Description
Rabbit polyclonal to Metabotropic Glutamate Receptor 3
Tested applications
WB
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human
Immunogen
Synthetic peptide:
TKVDEAEYM
, corresponding to amino acids 118-126 of Rat metabotropic Glutamate Receptor 3.
Run BLAST with
Positive control
Run BLAST with
Rat Cerebellar and cortical Membrane.
General notes
L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates
both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is
involved in most aspects of normal brain function and can be perturbed in many neuropathologic
conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors,
that have been divided into 3 groups on the basis of sequence homology, putative signal
transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5
and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and
GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are
linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1.0% BSA, Tris-glycine. pH 7.8
Purity
Whole antiserum
Primary antibody notes
L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates
both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is
involved in most aspects of normal brain function and can be perturbed in many neuropathologic
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conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors,
that have been divided into 3 groups on the basis of sequence homology, putative signal
transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5
and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and
GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are
linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab10309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/1000. Predicted molecular weight: 105.2 kDa. Using standard protocols the
antibody has only been shown to work in systems using highly expressing
transfected cells.
Target
Function
Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits
adenylate cyclase activity.
Sequence similarities
Belongs to the G-protein coupled receptor 3 family.
Cellular localization
Cell membrane.
Anti-Metabotropic Glutamate Receptor 3 antibody images
Predicted band size : 105.2 kDa
ab10309 at 1/1000 dilution by Western blot.
Left image: rat cerebellar membrane. Right
Western blot - Metabotropic Glutamate Receptor
image: rat cortical membrane.
3 antibody (ab10309)
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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