Product datasheet
Anti-Metabotropic Glutamate Receptor 2+3 antibody
ab6438
18 Abreviews 3 References 2 Images
Overview
Product name
Anti-Metabotropic Glutamate Receptor 2+3 antibody
Description
Rabbit polyclonal to Metabotropic Glutamate Receptor 2+3
Tested applications
IHC-P, IHC-Fr, WB, IP
Species reactivity
Reacts with: Mouse, Rat, Chicken, Cat
Predicted to work with: all Mammals
Immunogen
This antibody was raised against a synthetic peptide corresponding to the 13 c-terminus amino
acids of mGluR2/3 with an N-appended lysine coupled to thyroglobulin using glutaraldehyde.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Tris - glycine, 0.1% sodium azide
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab6438 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr
1/500.
WB
1/1000. Detects a band of approximately 100, 200 kDa.
IP
1/100.
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Target
Relevance
L glutamate is the major excitatory neurotransmitter in the central nervous system and activates
both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is
involved in most aspects of normal brain function and can be perturbed in many neuropathologic
conditions. The metabotropic glutamate receptors are a family of G protein coupled receptors,
that have been divided into 3 groups on the basis of sequence homology, putative signal
transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5
and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and
GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. The activity of GRM2 and
GRM3 receptors are mediated by a G-protein that inhibits adenylate cyclase activity.
Cellular localization
Integral membrane protein.
Anti-Metabotropic Glutamate Receptor 2+3 antibody images
ab6438 staining the Metabotropic Glutamate
Receptor 2+3 in Chicken brain tissue
sections by IHC-Fr (Frozen sections). Tissue
samples were fixed with paraformaldehyde
and blocked with 5% serum for 1 hour 30
minutes at 20°C. The sample was incubated
with primary antibody (1/100) at 4°C for 8
hours. Ab6720 (1/400) was used as
secondary antibody.
Immunohistochemistry (Frozen sections) Metabotropic Glutamate Receptor 2+3 antibody
(ab6438)
This image is courtesy of an anonymous Abreview
ab6438 staining Metabotropic Glutamate
Receptor 2+3 in rat retinal tissue by
Immunohistochemistry (Frozen sections).
Tissue was fixed in paraformaldehyde,
blocked using 10% serum for 20 minutes at
24°C, then incubated with ab6438 at a 1/100
dilution for 16 hours at 4°C. The secondary
used was an Alexa-Fluor 488 (green)
conjugated donkey anti-rabbit polyclonal,
Immunohistochemistry (Frozen sections) - Anti-
used at a 1/1000 dilution. Counterstained with
Metabotropic Glutamate Receptor 2+3 antibody
Hoechst 33258 (blue).
(ab6438)
Image courtesy of an anonymous Abreview.
GCL - ganglion cell layer
IPL - inner plexiform layer
INL - inner nuclear layer
OPL - outer plexiform layer
ONL - outer nuclear layer
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