Product datasheet
Anti-ULK1 (phospho S757) antibody ab156920
2 Images
Overview
Product name
Anti-ULK1 (phospho S757) antibody
Description
Rabbit polyclonal to ULK1 (phospho S757)
Tested applications
WB, ELISA
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Chimpanzee, Macaque Monkey, Orangutan
Immunogen
Synthetic peptide corresponding to Human ULK1 aa 700-800 (phospho S757) conjugated to
Keyhole Limpet Haemocyanin (KLH).
Database link: O75385
Positive control
This antibody gave a positive signal in A431 and A431 EGF treated (500mg) whole cell lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a
stabilising agent. If you would like information about the formulation of a specific lot, please
contact our scientific support team who will be happy to help.
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab156920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
1
Application
Abreviews
WB
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 112 kDa
(predicted molecular weight: 112 kDa).
ELISA
Use a concentration of 1 µg/ml.
Target
Function
Involved in autophagy. Required for autophagosome formation (By similarity). Target of the TOR
kinase signaling pathway that regulates autophagy through the control of phosphorylation status
of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By
similarity). Phosphorylates ATG13/KIAA0652. Involved in axon growth (By similarity). Plays an
essential role in neurite extension of cerebellar granule cells.
Tissue specificity
Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas,
brain, placenta, liver, kidney, and lung.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1
subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following
treatment with rapamycin.
Cellular localization
Cytoplasm > cytosol. Preautophagosomal structure. Under starvation conditions, is localized to
puncate structures primarily representing the isolation membrane that sequesters a portion of
the cytoplasm resulting in the formation of an autophagosome.
Anti-ULK1 (phospho S757) antibody images
2
All lanes : Anti-ULK1 (phospho S757)
antibody (ab156920) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma
cell line) Whole Cell Lysate
Lane 2 : EGF-Stimulated A431 Whole Cell
Lysate
Lane 3 : A431 (Human epithelial carcinoma
cell line) Whole Cell Lysate with Immunising
peptide at 1 µg/ml
Lane 4 : EGF-Stimulated A431 Whole Cell
Western blot - Anti-ULK1 (phospho S757)
Lysate with Immunising peptide at 1 µg/ml
antibody (ab156920)
Lane 5 : A431 (Human epithelial carcinoma
cell line) Whole Cell Lysate with Control
peptide at 1 µg/ml
Lane 6 : EGF-Stimulated A431 Whole Cell
Lysate with Control peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 112 kDa
Observed band size : 112 kDa
Additional bands at : 130 kDa (possible
non-specific binding).
Exposure time : 8 minutes
This blot was produced using a 10% Bis-tris
gel under the MOPS buffer system. The gel
was run at 200V for 50 minutes before being
transferred onto a Nitrocellulose membrane at
30V for 70 minutes. The membrane was then
blocked for an hour using 5% Bovine Serum
Albumin before being incubated with
ab156920 overnight at 4°C. Antibody binding
was detected using an anti-rabbit antibody
conjugated to HRP, and visualised using ECL
development solution.
3
ab156920 was tested using an Indirect ELISA
approach. The wells were coated with peptide
(1µg/ml at 100µl/well) overnight at 4°C,
followed by a 5% BSA blocking step for 1
hour at room temperature. The primary Ab
was then added at a dilution range of 10.00025µg/ml (100µl/well) for 1hr at room
temperature. A HRP-conjugated anti-rabbit
IgG (heavy and light chain) was used as a
secondary antibody at 1:20,000 dilution for
1hr at room temperature.
ELISA - Anti-ULK1 (phospho S757) antibody
(ab156920)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
4