Overview
Product name
Description
Specificity
Tested applications
Species reactivity
Immunogen
Positive control
Anti-VAMP8 antibody
Rabbit polyclonal to VAMP8
Reacts specifically with the 10 kDa protein of human CaCo-2, rat PC12 and dog MDCK cell extracts.
WB, IP, ICC/IF, IHC-P
Reacts with: Human
Predicted to work with: Dog
N-terminal synthetic peptide (Human).
human CaCo-2, rat PC12 and dog MDCK cell extracts. This antibody also gave a positive signal in the following Human whole cell lysates: A431; HEK293; HeLa.
Properties
Form
Storage instructions
Storage buffer
Purity
Clonality
Isotype
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -
80°C. Avoid freeze / thaw cycle.
Preservative: None
Constituents: Whole Serum
Whole antiserum
Polyclonal
IgG
Applications
Our Abpromise guarantee covers the use of ab6186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
WB
IP
ICC/IF
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Application
IHC-P
Application notes
Target
Function
Tissue specificity
Sequence similarities
Cellular localization
Abreviews Notes
IF: 1/50.
IM: Use at an assay dependant dilution.
IP: Use at an assay dependant dilution.
WB: 1/200 - 1/1000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.
Platelets.
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Membrane.
Anti-VAMP8 antibody images
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Western blot - Anti-VAMP8 antibody (ab6186)
All lanes : Anti-VAMP8 antibody (ab6186) at
1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080 ) at 1/5000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 11 kDa
Observed band size : 17 kDa
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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