Product datasheet
Anti-KCNIP4 antibody [EPR18314] ab203831
6 Images
Overview
Product name
Anti-KCNIP4 antibody [EPR18314]
Description
Rabbit monoclonal [EPR18314] to KCNIP4
Tested applications
WB, IP
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Recombinant fragment within Human KCNIP4 aa 1 to the C-terminus. The exact sequence is
proprietary.
Database link: Q6PIL6
Run BLAST with
Run BLAST with
Positive control
WB: Human fetal brain lysate; Mouse and rat brain lysates; Rat and mouse cerebellum lysates;
Human cerebellum and midbrain lysates. IP: Mouse brain whole cell lysate.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Purity
Protein A purified
Clonality
Monoclonal
Clone number
EPR18314
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab203831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 29
kDa).
IP
1/50.
Target
Function
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium
channels. Probably modulates channels density, inactivation kinetics and rate of recovery from
inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates
KCND2/Kv4.2 and KCND3/Kv4.3 currents.
Tissue specificity
Predominantly expressed in brain.
Sequence similarities
Belongs to the recoverin family.
Contains 4 EF-hand domains.
Domain
The KIS (K-channel inactivation suppressor) domain is required for converting A-type Kv4
current to a slowly inactivating delayed rectifier potassium current.
Cellular localization
Cell membrane.
Anti-KCNIP4 antibody [EPR18314] images
All lanes : Anti-KCNIP4 antibody
[EPR18314] (ab203831) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
Western blot - Anti-KCNIP4 antibody [EPR18314]
Anti-Rabbit IgG (HRP), specific to the non-
(ab203831)
reduced form of IgG at 1/1000 dilution
Predicted band size : 29 kDa
Observed band size : 28 kDa
Exposure time : 1 minute
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-KCNIP4 antibody
[EPR18314] (ab203831) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat kidney lysate
Western blot - Anti-KCNIP4 antibody [EPR18314]
Lane 8 : Rat spleen lysate
(ab203831)
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
at 1/50000 dilution
Predicted band size : 29 kDa
Observed band size : 28 kDa
Exposure time : 15 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-KCNIP4 antibody
[EPR18314] (ab203831) at 1/1000 dilution
Lane 1 : Rat cerebellum lysate
Lane 2 : Mouse cerebellum lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the nonreduced form of IgG at 1/1000 dilution
Western blot - Anti-KCNIP4 antibody [EPR18314]
(ab203831)
Predicted band size : 29 kDa
Observed band size : 28 kDa
Exposure time : 15 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
3
Anti-KCNIP4 antibody [EPR18314]
(ab203831) at 1/10000 dilution + Human
cerebellum lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the nonreduced form of IgG at 1/10000 dilution
Predicted band size : 29 kDa
Observed band size : 28 kDa
Western blot - Anti-KCNIP4 antibody [EPR18314]
(ab203831)
Exposure time : 1 second
Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-KCNIP4 antibody [EPR18314]
(ab203831) at 1/10000 dilution + Human
midbrain lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the nonreduced form of IgG at 1/10000 dilution
Predicted band size : 29 kDa
Observed band size : 28 kDa
Western blot - Anti-KCNIP4 antibody [EPR18314]
(ab203831)
Exposure time : 15 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
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KCNIP4 was immunoprecipitated from 1mg
of Mouse brain whole cell lysate with
ab203831 at 1/50 dilution. Western blot was
performed from the immunoprecipitate using
ab203831 at 1/1000 dilution. Anti-Rabbit IgG
(HRP), specific to the non-reduced form of
IgG, was used as secondary antibody at
1/1500.
Lane 1: Mouse brain whole cell lysate 10ug
(Input). Lane 2: ab203831 IP in Mouse brain
Immunoprecipitation - Anti-KCNIP4 antibody
whole cell lysate. Lane 3: Rabbit monoclonal
[EPR18314] (ab203831)
IgG (ab172730) instead of ab203831 in
Mouse brain whole cell lysate.
Blocking and dilution buffer and
concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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