Product datasheet
Anti-FOG1 antibody ab86281
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Overview
Product name
Anti-FOG1 antibody
Description
Rabbit polyclonal to FOG1
Tested applications
WB
Species reactivity
Reacts with: Human
Predicted to work with: Cow
Immunogen
Synthetic peptide corresponding to a region within the internal amino acids 755-804
(PPPPPGHAPA PESPRPGSGS GSGPGLAPAR SPGPAADGPI DLSKKPRRPL) of Human
FOG1 (NP_722520).
Run BLAST with
Positive control
Run BLAST with
Fetal muscle lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab86281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 104 kDa
(predicted molecular weight: 104 kDa). Good results were obtained when
blocked with 5% non-fat dry milk in 0.05% PBS-T.
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Target
Function
Transcription regulator that plays an essential role in erythroid and megakaryocytic cell
differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription
factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or
repress transcriptional activity, depending on the cell and promoter context. The heterodimer
formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B,
alpha- and beta-globin, while it represses expression of KLF1. May be involved in regulation of
some genes in gonads. May also be involved in cardiac development, in a non-redundant way
with ZFPM2/FOG2.
Tissue specificity
Mainly expressed in hematopoietic tissues. Also expressed in adult cerebellum, stomach, lymph
node, liver and pancreas. Expressed in fetal heart, liver and spleen.
Sequence similarities
Belongs to the FOG (Friend of GATA) family.
Contains 4 C2H2-type zinc fingers.
Contains 5 C2HC-type zinc fingers.
Domain
The CCHC-type zinc fingers 1, 5, 6 and 9 directly bind to GATA-type zinc fingers. The Tyr
residue adjacent to the last Cys of the CCHC-type zinc finger is essential for the interaction with
GATA-type zinc fingers.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Anti-FOG1 antibody images
Anti-FOG1 antibody (ab86281) at 1 µg/ml
(5% skim milk/ PBS buffer) + fetal muscle
lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000
dilution
Predicted band size : 104 kDa
Observed band size : 104 kDa
Western blot - FOG1 antibody (ab86281)
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