Product datasheet
Anti-Neurexin 1 antibody ab77596
2 Images
Overview
Product name
Anti-Neurexin 1 antibody
Description
Goat polyclonal to Neurexin 1
Specificity
This antibody will detect Neurexin 1 beta and Neurexin 1 alpha (isoform 1a, 2a and 3a).
Tested applications
WB, ELISA, ICC/IF
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog
Immunogen
Synthetic peptide corresponding to Human Neurexin I beta (C terminal). The immunogen
sequence is aa419-432 of Neurexin 1 beta (P58400), aa1454-1467 of Neurexin 1 alpha
(Q9ULB1), aa1476-1489 of Neurexin 1 alpha (Q9ULB1-2), and aa1524-1537 of Neurexin 1
alpha (Q9ULB1-3).
Sequence:
KEKQPSSAKSSNKN
Run BLAST with
Positive control
Run BLAST with
Human brain (cerebellum) lysate IF/ICC: SKNSH cell line.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity
chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab77596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
WB
ELISA
ICC/IF
Application notes
ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/32000
WB: Use at a concentration of 0.05 - 0.1 µg/ml. Predicted molecular weight: 162 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Cell surface protein involved in cell-cell-interactions, exocytosis of secretory granules and
regulation of signal transmission. Function is isoform-specific. Alpha-type isoforms have a long
N-terminus with six laminin G-like domains and play an important role in synaptic signal
transmission. Alpha-type isoforms play a role in the regulation of calcium channel activity and
Ca(2+)-triggered neurotransmitter release at synapses and at neuromuscular junctions. They
play an important role in Ca(2+)-triggered exocytosis of secretory granules in pituitary gland.
They may effect their functions at synapses and in endocrine cells via their interactions with
proteins from the exocytotic machinery. Likewise, alpha-type isoforms play a role in regulating
the activity of postsynaptic NMDA receptors, a subtype of glutamate-gated ion channels. Both
alpha-type and beta-type isoforms may play a role in the formation or maintenance of synaptic
junctions via their calcium-dependent interactions (via the extracellular domains) with neuroligin
family members, CBLN1 or CBLN2. In vitro, triggers the de novo formation of presynaptic
structures. May be involved in specification of excitatory synapses. Alpha-type isoforms were
first identified as receptors for alpha-latrotoxin from spider venom.
Tissue specificity
Brain.
Sequence similarities
Belongs to the neurexin family.
Contains 3 EGF-like domains.
Contains 6 laminin G-like domains.
Post-translational
modifications
N-glycosylated.
O-glycosylated.
Cellular localization
Cell membrane. Cell junction, synapse. Localized on the pre-synaptic membrane.
Form
The neurexin genes use alternate promoters, splice sites and exons to encode potentially
hundreds or thousands of mRNA transcripts.
Anti-Neurexin 1 antibody images
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ab77596 stained SKNSH cells. The cells
were 100% methanol fixed for 5 minutes at 20°C and then incubated in 1%BSA / 0.3M
glycine in 0.1% PBS-Tween for 1hour at room
temperature to permeabilise the cells and
block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab77596 at 10µg/ml)
overnight at +4°C. The secondary antibody
Immunocytochemistry/ Immunofluorescence -
(pseudo-colored green) was
Anti-Neurexin I beta antibody (ab77596)
Donkey Anti-Goat IgG H&L (Alexa Fluor®
488) preadsorbed (ab150133) used at a
1/1000 dilution for 1hour at room temperature.
Alexa Fluor® 594 WGA was used to label
plasma membranes (pseudo-colored red) at
a 1/200 dilution for 1hour at room
temperature. DAPI was used to stain the cell
nuclei (pseudo-colored blue) at a
concentration of 1.43µM for 1hour at room
temperature.
Anti-Neurexin 1 antibody (ab77596) at 0.05
µg/ml + human brain (cerebellum) lysate in
RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 162 kDa
Observed band size : 150 kDa
Western blot - Neurexin I beta antibody (ab77596)
Primary antibody incubation: 1 hour
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