Product datasheet
Anti-Neurexin II alpha antibody ab34245
1 Abreviews 2 References 3 Images
Overview
Product name
Anti-Neurexin II alpha antibody
Description
Rabbit polyclonal to Neurexin II alpha
Tested applications
IHC-P, WB, ICC/IF
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Rat Neurexin II
alpha.Read Abcam's proprietary immunogen policy(Peptide available as ab34449.)
Positive control
normal adult human cerebral cortex SKNSH cell line
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab34245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
1/700. Perform heat mediated antigen retrieval via the pressure cooker method
before commencing with IHC staining protocol.
WB
1/400.
ICC/IF
Use a concentration of 5 µg/ml.
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Target
Relevance
Neurexin 2 alpha belongs to the Neurexin family, a group of neuronal proteins that function as cell
adhesion molecules during synaptogenesis and intracellular signaling. Mammalian genomes
contain just three neurexin genes. However, many (>200) neurexin isoforms may be expressed
through the use of two alternative promoters and alternative splicing at up to five different
positions.
Cellular localization
Membrane; single-pass type I membrane protein.
Anti-Neurexin II alpha antibody images
(Image courtesy of Human Protein Atlas)
ab34245 staining Neurexin II alpha protein in
normal adult human cerebral cortex. Brown
color indicates presence of protein, blue color
shows cell nuclei. Paraffin embedded human
cerebral cortex tissue was incubated with
ab34245 (1/700 dilution) for 30 mins at room
temperature. Antigen retrieval was performed
by heat induction in citrate buffer pH 6.
.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Neurexin II alpha
antibody (ab34245)
Anti-Neurexin II alpha antibody (ab34245) at
1/400 dilution + whole cell lysate prepared
from murine N2A cells at 20 µg
Secondary
Goat polyclonal conjugated to Dylight 800 at
1/20000 dilution
Performed under reducing conditions.
Predicted band size : 185 kDa
Western blot - Neurexin II alpha antibody
Observed band size : 160 kDa
(ab34245)
Image courtesy of an anonymous Abreview.
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ICC/IF image of ab34245 stained SKNSH
cells. The cells were 4% formaldehdye fixed
(10 min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab34245, 1µg/ml) overnight
at +4°C. The secondary antibody (green) was
Immunocytochemistry/ Immunofluorescence -
ab96899, DyLight® 488 goat anti-rabbit IgG
Anti-Neurexin II alpha antibody (ab34245)
(H+L) used at a 1/250 dilution for 1h. Alexa
Fluor® 594 WGA was used to label plasma
membranes (red) at a 1/200 dilution for 1h.
DAPI was used to stain the cell nuclei (blue)
at a concentration of 1.43µM.
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