Product datasheet
Anti-Laminin gamma 1 antibody [A5] - BSA and Azide
free ab80580
3 References 2 Images
Overview
Product name
Anti-Laminin gamma 1 antibody [A5] - BSA and Azide free
Description
Rat monoclonal [A5] to Laminin gamma 1 - BSA and Azide free
Specificity
This antibody uniformly stains all human and murine basement membranes.
Tested applications
IP, IHC-P, IHC-Fr, ICC/IF
Species reactivity
Reacts with: Mouse, Human
Immunogen
Murine EHS laminin preparation.
Positive control
LS174T cells. Normal colon or colon carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
Constituents: 10mM PBS, pH 7.4
Purity
Protein G purified
Clonality
Monoclonal
Clone number
A5
Isotype
IgG2a
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab80580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IP
IHC-P
IHC-Fr
1
Application
Abreviews
Notes
ICC/IF
Application notes
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 µg/ml.
IHC Acetone-fixed Fr: Use at a concentration of 2 µg/ml.
IP: Use at 2µg/mg of lysate. (Native and denatured)
Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine
testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37°C.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment,
migration and organization of cells into tissues during embryonic development by interacting with
other extracellular matrix components.
Tissue specificity
Found in the basement membranes (major component).
Sequence similarities
Contains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a
coiled coil structure.
Domains VI and IV are globular.
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane.
Anti-Laminin gamma 1 antibody [A5] - BSA and Azide free images
Frozen human tonsil stained with ab80580 at
2µg/ml using peroxidase-conjugate and DAB
chromogen. Note basement membrane and
cell surface staining of blood vessels.
Immunohistochemistry (Frozen sections) - AntiLaminin gamma 1 [A5] antibody - BSA and Azide
free (ab80580)
2
Native IP using LS174T cells:
IP 2µg/mg lysate.
WB at 2µg/ml.
Immunoprecipitation - Anti-Laminin gamma 1 [A5]
antibody - BSA and Azide free (ab80580)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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