Product datasheet
Anti-Neuroligin 2 antibody ab36602
2 Abreviews 2 Images
Overview
Product name
Anti-Neuroligin 2 antibody
Description
Rabbit polyclonal to Neuroligin 2
This product is a fast track antibody. It has been affinity purified and shows high
titre values against the immunizing peptide by ELISA.
Read the terms of use »
Species reactivity
Predicted to work with: Mouse, Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human
Neuroligin 2.Read Abcam's proprietary immunogen policy(Peptide available as ab36601.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully
characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA).
Fast track terms of use
Application
ELISA
Abreviews
Notes
Use at an assay dependent concentration. This antibody gave a positive result in
ELISA against the immunizing peptide (ab36601).
IHC-FoFr
1/300.
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Target
Relevance
Neuroligins are cell adhesion proteins that are thought to instruct the formation and alignment of
synaptic specializations. The three known rodent neuroligin isoforms share homologous
extracellular acetylcholinesterase-like domains that bridge the synaptic cleft and bind betaneurexins. All neuroligins have identical intracellular C-terminal motifs that bind to PDZ domains
of various target proteins. Neuroligin 2 is exclusively localized to inhibitory synapses in rat brain
and dissociated neurons. In immature neurons, neuroligin 2 is found at synapses and also at
GABAA receptor aggregates that are not facing presynaptic termini.
Cellular localization
Cell Membrane
Anti-Neuroligin 2 antibody images
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
ab36602 staining Neuroligin 2 in mouse brain
tissue sections by Immunohistochemistry
(PFA perfusion fixed frozen sections). Tissue
samples were fixed by perfusion with
paraformaldehyde, cut into 20 micron slices,
permeablized with 0.1% Triton-X, blocked
with 5% serum and 2.5%BSA for 60 minutes
at 24°C. The sample was incubated with
primary antibody (1/400) at 4°C for 16 hours.
Immunohistochemistry (PFA perfusion fixed
frozen sections) - Anti-Neuroligin 2 antibody
(ab36602)
An Alexa Fluor® 568-conjugated Goat antirabbit polyclonal (H+L) (1/1000) was used as
the secondary antibody.
This image is courtesy of an anonymous Abreview.
IHC-FoFr image of Neuroligin 2 (ab36602)
staining on Rat Spinal Cord. The sections
used came from animals perfused fixed with
Paraformaldehyde 4%, in phosphate buffer
0.2M. Following postfixation in the same
Immunohistochemistry (PFA perfusion fixed
fixative overnight, the tissues were
frozen sections) - Neuroligin 2 antibody (ab36602)
cryoprotected in sucrose 30% overnight.
Sophie Pezet, ESPCI, France
Tissues were then cut using a cryostat and the
immunostainings were preformed using the
‘free floating’ technique. Image A shows the
staining observed at the level of the lateral
part of the dorsal horn, showing stained
lamina II neurons (arrows). Image B shows the
staining observed lamina X.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Terms and conditions
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Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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