Product datasheet
Anti-Robo2 antibody ab75014
3 Abreviews 2 Images
Overview
Product name
Anti-Robo2 antibody
Description
Rabbit polyclonal to Robo2
Specificity
ab75014 has shown to not cross react with human Robo1 or Robo3 protein.
Tested applications
WB
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1250 - 1350 of Mouse
Robo2.Read Abcam's proprietary immunogen policy(Peptide available as ab75013.)
Positive control
This antibody gave a positive signal in the following tissue lysates: Rat Liver Mouse Liver
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab75014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 171 kDa
(predicted molecular weight: 161 kDa).Can be blocked with Mouse Robo2
peptide (ab75013).
Target
1
Function
Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in
cellular migration, including axonal navigation at the ventral midline of the neural tube and
projection of axons to different regions during neuronal development.
Involvement in disease
Defects in ROBO2 are the cause of vesicoureteral reflux type 2 (VUR2) [MIM:610878]. VUR is a
complex, genetically heterogeneous developmental disorder characterized by the retrograde
flow of urine from the bladder into the ureter and is associated with reflux nephropathy, the cause
of 15% of end-stage renal disease in children and young adults.
Note=A chromosomal aberration involving ROBO2 is a cause of multiple congenital
abnormalities, including severe bilateral VUR with ureterovesical junction defects. Translocation
t(Y;3)(p11;p12) with PCDH11Y. This translocation disrupts ROBO2 and produces dominantnegative ROBO2 proteins that abrogate SLIT-ROBO signaling in vitro.
Sequence similarities
Belongs to the immunoglobulin superfamily. ROBO family.
Contains 3 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localization
Membrane.
Anti-Robo2 antibody images
Lanes 1 - 3 : Anti-Robo2 antibody (ab75014)
at 2 µg/ml
Lanes 4 - 6 : Mouse Monoclonal to V5 at
1/1000 dilution
Lane 1 : HEK293 cells expressing Robo1
Lane 2 : HEK293 cells expressing Robo2-V5
Lane 3 : HEK293 cells expressing Robo3
Lane 4 : HEK293 cells expressing Robo1
Lane 5 : HEK293 cells expressing Robo2-V5
Western blot - Robo2 antibody (ab75014)
Lane 6 : HEK293 cells expressing Robo3
Image Courtsey Alain Chedotal, INSTITUT DE LA
VISION, France
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 3 : Anti-Rabbit HRP at 1/20000
dilution
Lanes 4 - 6 : Anti-Mouse HRP
Predicted band size : 161 kDa
Image Courtsey Alain Chedotal, INSTITUT
DE LA VISION, France
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All lanes : Anti-Robo2 antibody (ab75014) at
1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
at 1/10000 dilution
Western blot - Anti-Robo2 antibody (ab75014)
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 161 kDa
Exposure time : 20 minutes
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