Product datasheet
Anti-Robo1 antibody ab111726
2 Abreviews 2 Images
Overview
Product name
Anti-Robo1 antibody
Description
Goat polyclonal to Robo1
Specificity
ab11726 does not cross react with Robo2 or Robo3
Tested applications
WB
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Dog
Immunogen
Synthetic peptide:
QKARPAKKQKHQ
by a Cysteine residue linker, corresponding to internal sequence amino acids 1417-1428 of
Mouse Robo1 (NP_062286.2)
Run BLAST with
Positive control
Run BLAST with
Mouse Fetal brain lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity
chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab111726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
WB
Notes
Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 200
kDa (predicted molecular weight: 181 kDa).
Target
Function
Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular
migration, including axonal navigation at the ventral midline of the neural tube and projection of
axons to different regions during neuronal development. In axon growth cones, the silencing of
the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex.
May be required for lung development.
Tissue specificity
Widely expressed, with exception of kidney.
Sequence similarities
Belongs to the immunoglobulin superfamily. ROBO family.
Contains 3 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Post-translational
modifications
Ubiquitinated. May be deubiquitinated by USP33.
Cellular localization
Membrane.
Anti-Robo1 antibody images
Anti-Robo1 antibody (ab111726) at 0.05
µg/ml + Mouse Fetal brain lysate at 35 µg
developed using the ECL technique
Predicted band size : 181 kDa
Western blot - Robo1 antibody (ab111726)
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All lanes : Anti-Robo1 antibody (ab111726)
at 500 µg/ml
Lane 1 : Blank
Lane 2 : HEK293 cells expressing Robo1
Lane 3 : HEK293 cells expressing Robo2
Lane 4 : HEK293 cells expressing Robo3
Lysates/proteins at 20 µg per lane.
Secondary
Western blot - Anti-Robo1 antibody (ab111726)
Anti-goat IgG conjugated to HRP at 1/10000
dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 181 kDa
Observed band size : 250 kDa
Exposure time : 3 minutes
Alain Chedotal, Dépt. of Dev, Institut Vision,
France
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