Product datasheet
Anti-Calbindin antibody [CB-955] ab82812
3 Abreviews 8 References 2 Images
Overview
Product name
Anti-Calbindin antibody [CB-955]
Description
Mouse monoclonal [CB-955] to Calbindin
Specificity
This antibody is specific to a protein of 28 kDa. Calbindin-D-28K is also known as vitamin Ddependent calcium-binding protein or cholecalcin. Calbindin D-28K has a broad tissue
distribution; however it exhibits a cell type-specific expression pattern. This antibody reacts
specifically with calbindin-D-28K in brain and kidney tissues from human, bovine, goat, porcine,
rabbit, dog, cat, rat, mouse, sheep, and guinea pig sources. It binds weakly to chicken calbindinD-28K.
Tested applications
ICC/IF, IHC-FoFr, IHC-P
Species reactivity
Reacts with: Mouse, Rat, Sheep, Rabbit, Goat, Guinea pig, Cow, Cat, Human, Pig
Immunogen
Purified bovine kidney calbindin-D-28K
Positive control
Kidney
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze /
thaw cycle.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Ascites
Purity
Ascites
Clonality
Monoclonal
Clone number
CB-955
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab82812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
ICC/IF
Abreviews
Notes
Use at an assay dependent concentration. PubMed: 20542987
1
Application
Abreviews
Notes
IHC-FoFr
1/2800.
IHC-P
1/15 - 1/30. Perform heat mediated antigen retrieval before commencing with
IHC staining protocol.
Target
Function
Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic
nucleotide phosphodiesterase.
Sequence similarities
Belongs to the calbindin family.
Contains 5 EF-hand domains.
Domain
This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for
calcium.
Anti-Calbindin antibody [CB-955] images
ab82812 staining rat brain (corpus
callosum) sections by IHC-FoFr. The animal
was perfused with 4% paraformaldehyde and
further post fixed with 4% paraformaldehyde
overnight. The tissues were cryoprotected
with 30% sucrose and sectioned using a
cryostat. Staining with ab82812 at a 1/2800
dilution in PBS-Triton (0.3%) with 0.02%
azide was performed for 18h at 25°C. A
donkey anti-mouse Alexa488 polyclonal
antibody at 1/1000 was used as the
Immunohistochemistry (PFA perfusion fixed
secondary antibody.
frozen sections) - Anti-Calbindin antibody [CB955] (ab82812)
This image is courtesy of an Abreview submitted by
Karine Thibault
2
ab82812 staining Calbindin in Mouse brain
tissue by Immunohistochemistry (PFA
perfusion fixed frozen sections).
Sections were fixed with paraformaldehyde,
permeabilized with 0.2% Triton X-100 and
blocked with 10% normal donkey serum + 0.3
M Glycine for 1 hour at 25°C; antigen retrieval
was by heat mediation in citrate buffer.
Immunohistochemistry (PFA perfusion fixed
Samples were incubated with primary
frozen sections) - Anti-Calbindin antibody [CB-
antibody (1/2500 in 5% serum + 0.2% Triton
955] (ab82812)
X-100 in PBS) for 18 hours at 4°C. An
This image is courtesy of an Abreview submitted by
Mr Lars Riecken
AlexaFluor® 488-conjugated Donkey antimouse IgG polyclonal (1/1000) was used as
the secondary antibody. Counterstained with
DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
3