Product datasheet
Anti-Zyxin antibody [EPR4302] ab109316
1 References 4 Images
Overview
Product name
Anti-Zyxin antibody [EPR4302]
Description
Rabbit monoclonal [EPR4302] to Zyxin
Tested applications
WB, IP, IHC-P, Flow Cyt, ICC/IF
Species reactivity
Reacts with: Mouse, Human
Immunogen
A synthetic peptide corresponding to residues in Human Zyxin.
Positive control
WB: HeLa, MCF7 and C2C12 cell lysates IHC-P: Human kidney tissue ICC/IF: HeLa cells
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these
species. Please contact us for more information.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR4302
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab109316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
WB
Notes
1/10000 - 1/50000. Detects a band of approximately 82 kDa (predicted
molecular weight: 61 kDa).
IP
1/10 - 1/100.
IHC-P
1/250 - 1/500. Perform antigen retrieval before commencing with IHC staining
protocol.
Flow Cyt
1/1000 - 1/10000. ab172730-Rabbit monoclonal IgG, is suitable for use as an
isotype control with this antibody.
ICC/IF
1/100 - 1/250.
Target
Relevance
Zyxin binds alpha-actinin and the CRP proteins and is implicated in the regulation and
modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal
transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. It has been
suggested that zyxin is involved in gene transcription by its association with transcription factors
in the nucleus.
Cellular localization
Cytoplasmic, cytoskeletal and Nuclear.
Anti-Zyxin antibody [EPR4302] images
Overlay histogram showing HeLa cells
stained with ab109316 (red line). The cells
were fixed with 80% methanol (5 min) and
then permeabilized with 0.1% PBS-Tween for
20 min. The cells were then incubated in 1x
PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein
interactions followed by the antibody
(ab109316, 1/10000 dilution) for 30 min at
Flow Cytometry - Anti-Zyxin antibody [EPR4302]
(ab109316)
22°C. The secondary antibody used was
Alexa Fluor® 488 goat anti-rabbit IgG (H&L)
(ab150077) at 1/2000 dilution for 30 min at
22°C. Isotype control antibody (black line)
was rabbit IgG (monoclonal) (0.1μg/1x106
cells) used under the same conditions.
Unlabelled sample (blue line) was also used
as a control. Acquisition of >5,000 events
were collected using a 20mW Argon ion laser
(488nm) and 525/30 bandpass filter.
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All lanes : Anti-Zyxin antibody [EPR4302]
(ab109316) at 1/10000 dilution
Lane 1 : HeLa lysate
Lane 2 : MCF7 lysate
Lane 3 : C2C12 lysate
Lysates/proteins at 10 µg per lane.
Western blot - Zyxin antibody [EPR4302]
(ab109316)
Predicted band size : 61 kDa
Observed band size : 82 kDa
Immunohistochemical analysis of Zyxin in
paraffin embedded Human kidney tissue,
using ab109316 at a 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Zyxin antibody
[EPR4302] (ab109316)
Immunofluorescent staining of Zyxin in HeLa
cells, using ab109316 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence Zyxin antibody [EPR4302] (ab109316)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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Terms and conditions
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