Product datasheet
Anti-TRAF5 antibody ab47539
3 Images
Overview
Product name
Anti-TRAF5 antibody
Description
Rabbit polyclonal to TRAF5
Tested applications
WB, IHC-P, IP
Species reactivity
Reacts with: Mouse, Rat, Human, Gerbil
Immunogen
Synthetic peptide:
MAHSEEQAAVPCAFIRQNSG
, corresponding to N terminal amino acids 1-20 of Mouse TRAF5
Run BLAST with
Positive control
Run BLAST with
Spleen, lymphoid, many cancer cell lines.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab47539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/1000 - 1/2000. Detects a band of approximately 65 kDa (predicted molecular
weight: 64 kDa).
IHC-P
Use at an assay dependent concentration.
IP
1/50 - 1/200.
1
Target
Function
Adapter protein and signal transducer that links members of the tumor necrosis factor receptor
family to different signaling pathways by association with the receptor cytoplasmic domain and
kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in
apoptosis.
Tissue specificity
Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood.
Sequence similarities
Belongs to the TNF receptor-associated factor family. A subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 2 TRAF-type zinc fingers.
Domain
The MATH/TRAF domain binds to receptor cytoplasmic domains.
Cellular localization
Cytoplasm.
Anti-TRAF5 antibody images
All lanes : Anti-TRAF5 antibody (ab47539)
at 1/2000 dilution
Lane 1 : Normal human testis tissue
Lane 2 : Normal human kidney tissue
Lane 3 : Normal human pancreas tissue
Lane 4 : Normal human liver tissue
Lane 5 : Normal human prostate tissue
Lane 6 : Normal human breast tissue
Western blot - TRAF5 antibody (ab47539)
Lane 7 : Normal human colon tissue
Lane 8 : Normal human thymus tissue
Lane 9 : Normal human lymph node tissue
Lane 10 : Normal human spleen tissue
Lane 11 : Normal human lung tissue
Lane 12 : Normal human aorta tissue
Lane 13 : Normal human epidermis tissue
Predicted band size : 64 kDa
Observed band size : 64 kDa
Formalin-fixed, paraffin-embedded mouse
spleen stained for TRAF5 expression using
ab47539 at 1:2000. Hematoxylin-eosin
counterstain. A1 is a higher magnification of
Immunohistochemistry (Formalin/PFA-fixed
A.
paraffin-embedded sections) - TRAF5 antibody
(ab47539)
2
Formalin-fixed, paraffin-embedded mouse
tissue stained for TRAF5 expression using
ab47539 at 1:2000. Hematoxylin-eosin
counterstain.
A, thymus.
B, bone marrow.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - TRAF5 antibody
(ab47539)
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