Product datasheet
Anti-Mast Cell Tryptase antibody [AA1] ab2378
29 Abreviews 12 References 8 Images
Overview
Product name
Anti-Mast Cell Tryptase antibody [AA1]
Description
Mouse monoclonal [AA1] to Mast Cell Tryptase
Specificity
This antibody reacts with mast cells distributed in skin, synovium, lung and heart. It does not bind
with any other cell type. Recognises human mast cell tryptase, both alpha and beta isoforms
Tested applications
ICC, WB, IHC-P, ICC/IF, IHC-R, ELISA
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cat, Dog, Monkey
Immunogen
Purified human lung Tryptase
Positive control
This antibody gave a positive signal in the following human tissue lysates: Lung; Tonsil; Skin.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20ºC.
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Purity
Protein G purified
Clonality
Monoclonal
Clone number
AA1
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Applications
Our Abpromise guarantee covers the use of ab2378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
ICC
Abreviews
Notes
Use at an assay dependent concentration.
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Application
Abreviews
WB
Notes
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 35-37 kDa
(predicted molecular weight: 31 kDa).
We recommend using 3% milk as the blocking agent for Western blot.
IHC-P
Use a concentration of 0.1 µg/ml.
ICC/IF
1/100.
IHC-R
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Target
Relevance
Mast cells contain a number of preformed chemical mediators such as histamine, chymase,
carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an
important marker of mast cell activation as well as an important mediator of inflammation.
Cellular localization
Secreted. Note: released from the secretory granules upon mast cell activation.
Anti-Mast Cell Tryptase antibody [AA1] images
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All lanes : Anti-Mast Cell Tryptase antibody
[AA1] (ab2378) at 5 µg/ml
Lane 1 : Lung (Human) Whole Cell Lysate normal adult 40-65 yrs (ab30281)
Lane 2 : Tonsil (Human) Whole Cell Lysate adult normal tissue (ab29615)
Lane 3 : Skin (Human) Tissue Lysate - adult
normal tissue (ab30166)
Lysates/proteins at 20 µg per lane.
Western blot - Anti-Mast Cell Tryptase antibody
[AA1] (ab2378)
Secondary
Goat Anti-Mouse IgG H&L (HRP)
preadsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 35-37 kDa
Exposure time : 20 minutes
Mast Cell Tryptase contains an 18-residue
signal sequence and a 12-residue activation
peptide. The protein also has two potential
glycosylation sites (Swissprot data). These
post-translational modifications might explain
the banding pattern observed. Abcam used
5% milk in TBS-T as a blocking agent for this
blot. We found that this blocking agent
removed more non-specific bands than BSA.
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ab2378 staining Mast Cell
Tryptase in Human Tonsil tissue sections by
Immunohistochemistry (IHC-P paraformaldehyde-fixed, paraffin-embedded
sections). Tissue was fixed with formaldehyde
and blocked with 1% BSA for 10 minutes at
21°C; antigen retrieval was by heat mediation
in a citric acid. Samples were incubated with
primary antibody (1/7500 in TBS/BSA/azide)
Immunohistochemistry (Formalin/PFA-fixed
for 2 hours at 21°C. A Biotin-conjugated Goat
paraffin-embedded sections) - Anti-Mast Cell
anti-mouse IgG polyclonal (1/250) was used
Tryptase antibody [AA1] (ab2378)
as the secondary antibody.
Image is courtesy of an AbReview submitted by Carl
Hobbs
ab2378 staining Mast Cell Tryptase in Human
nasal polyp and tonsil tissue sections by
Immunohistochemistry (resin-embedded
sections). Tissue was fixed with acetone and
inhibitors and embedded in GMA resin.
Samples were incubated with primary
antibody (1/2000 in Tris buffered saline) for
18 hours at 21°C. A Biotin-conjugated Rabbit
anti-mouse IgG polyclonal (1/1000) was used
Immunohistochemistry (Resin sections) - Mast
as the secondary antibody.
Cell Tryptase antibody [AA1] (ab2378)
This image is courtesy of an anonymous Abreview
ab2378 staining Mast Cell Tryptase in Mouse
RAW 264.7 cells by ICC/IF
(Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde,
permeabilized with 0.1% Triton X-100 and
blocked with 10% Super block for 1 hour at
22°C. Samples were incubated with primary
antibody (1/150 in PBS + 10% super block)
for 14 hours at 4°C. An Alexa Fluor® 568conjugated Goat anti-mouse IgG polyclonal
(1/500) was used as the secondary antibody
Immunocytochemistry/ Immunofluorescence Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
Image courtesy of an anonymous AbReview
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ab2378 staining Mast Cell Tryptase in Rat
Lung tissue sections by
Immunohistochemistry (IHC-P paraformaldehyde-fixed, paraffin-embedded
sections). Tissue was fixed with formaldehyde
and antigen retrieval was by heat mediation in
a citrate buffer. Samples were incubated with
primary antibody (1/200) for 16 hours at 4°C.
A Biotin-conjugated Goat anti-mouse IgG
Immunohistochemistry (Formalin/PFA-fixed
polyclonal (1/500) was used as the secondary
paraffin-embedded sections) - Anti-Mast Cell
antibody.
Tryptase antibody [AA1] (ab2378)
Image is courtesy of an AbReview submitted by Dr
Fancois Daubeuf
ab2378 staining Mast Cell
Tryptase in Mouse Lung (LPS
inflammation) tissue sections by
Immunohistochemistry (IHC-P paraformaldehyde-fixed, paraffin-embedded
sections). Tissue was fixed with
paraformaldehyde and blocked
with 4% M.O.M Ig blocking for 1 hour at 20°C;
antigen retrieval was by heat mediation in a
Immunohistochemistry (Formalin/PFA-fixed
citric acid. Samples were incubated with
paraffin-embedded sections) - Anti-Mast Cell
primary antibody (1/200 in blocking buffer)
Tryptase antibody [AA1] (ab2378)
for 16 hours at 4°C. A Biotin-
Image courtesy of an AbReview submitted by Dr
Francois Daubeuf
conjugated Rabbit anti-mouse IgG polyclonal
(1/100) was used as the secondary antibody.
ab2378 staining formalin fixed paraffinembedded human colon tissue sections cut
at 1 micron. The section was subjected to
heat mediated antigen retrieval in Dako
Target Retrieval Solution and permeabilized
in Triton-X prior to blocking in 6% BSA for 2
hours at 24°C. The primary anitbody was
diluted 1/250 and incubated with the sample
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Mast Cell Tryptase
antibody [AA1] (ab2378)
for 16 hours at 5°C. The secondary antibody
was ab7064 (diluted 1/2000) and the slide
was counterstained with DAPI.
This image is courtesy of an Abreview submitted by
Dr Tom Donndelinger
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ab2378 staining Mast Cell Tryptase in Human
mast cells by ICC/IF
(Immunocytochemistry/immunofluorescence).
Cells were fixed with methanol, permeabilized
with Saponin 0.1% in PBS and blocked with
4% serum for 30 minutes at 25°C. Samples
were incubated with primary antibody (1/100
in blocking buffer) for 14 hours at 4°C. A
Texas Red®-conjugated Goat anti-mouse
Immunocytochemistry/ Immunofluorescence -
polyclonal (1/100) was used as the secondary
Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
antibody.
This image is courtesy of an anonymous Abreview
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