AN ABSTRACT OF THE THESIS OF in Donald A. Horneck for the degree of Soil Science presented on Doctor of Philosophy April 25, 1994 Title: NUTRIENT MANAGEMENT AND CYCLING IN GRASS SEED CROPS Redacted for Privacy Abstract approved: John Hart Grass seed production in Oregon's Willamette Valley traditionally relied on open field burning for straw residue disposal and nutrient recycling. Changes in residue management from open field burning to methods that remove straw coincided with rapidly declining K soil test values. A survey of grass seed fields showed that many fields had low soil pH. In contrast, P and K fertilization continued in spite of soil test values 3 times critical levels. These findings raised questions about fertilization and nutrition of grass grown for seed. In an effort to answer questions about plant nutrient demand, soil nutrient supply and liming for grass seed production, three field studies were implemented. The first study measured nutrient and dry matter accumulation by 5 grass species. Nitrogen, phosphorus, and potassium were taken up in advance of dry matter production in perennial ryegrasses and orchard grass. In contrast nutrient accumulation by Kentucky bluegrass, tall and fine fescues coincided with dry matter production. The second study examined K nutrition of grass grown for seed. Fertilizer K or K soil test level did not influence seed yield. High K soil test levels produced high straw K concentration. Tall fescue accumulated a maximum of 300 kg K ha1 where as perennial ryegrass accumulated only 125 kg K ha'. The difference in K uptake of these grasses was a function of both dry matter and K concentration. The third study investigated P and lime applications to grass grown for seed. P applications had no effect on seed yield or tissue P, hut increased inorganic P in soil solution and conventional soil tests. Cool season grasses took up between 17 and 34 kg P ha. Lime increased soil pH but did not increase seed yield. Lime increased soil solution pH and bases while decreasing metallic cation concentration. This study did not provide a justification for P fertilization when soil test P is above 25 mg kg P. Lime had little effect on Pi and conventional soil tests. NUTRIENT MANAGEMENT AND CYCLING IN GRASS SEED CROPS Donald A. Horneck A THESIS submitted to Oregon State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy Completed April 25, 1994 Commencement June 1995 APPROVED: Redacted for Privacy fessor of Crop and Soil Science in charge of major Redacted for Privacy Chairman of department of Crop and Soil Science Redacted for Privacy I )ean of Graduate S Date thesis presented: Typed by: April 25. 1994 Donald A. Horneck ACKNOWLEDGEMENTS I would like to thank first of all John Hart my advisor, friend, supervisor... He has withstood my continued harassment and given me needed guidance. The few non gray hairs his kids left I was more than willing to convert. Not only did we finish my degree, but the soil testing laboratory moved, merged and survived. We conquered a quadrathon, ate lots of Joan's goodies after races, survived videos and an insurmountable quantity of noon hour bulishit. We had lots of fun, which is what life is about. I would also like to thank my committee members. Neil Christensen for his availability and willingness to act as a sounding board for some of my crazier ideas. Bill Young for his cooperation on several articles that make up this thesis. His interest and help greatly assisted my work. Mike Schulyer and Pete Nelson for the quality of their classes and willingness to assist graduate students outside their department. The Soil testing crew, currently Barb, Robert and Glenda, have been good friends and a pleasure to work with. Their dedication and standards of quality have made my tenure at OSU rew arding. Their work in the laboratory was the basis from which my reputation in the fertilizer industry was built, thanks. The Soils department, secretaries, faculty and fellow graduate students have been helpful and a joy to work with. Most importantly, I would like to thank my wife Vicki. She has patently endured moves, family stress and long hours so that I could fulfill a dream and finish my degree. Between getting my M.S. at Illinois and a Phd at OSU, I'm sure she felt my attendance at school would never end. Her love, dedication and understanding has made my stay at OSU possible. During the process three children, Amethyst. Abigail and Brian. were born and accumulated 10 years. Their demands have been enjoyable and a constant challenge. Through it all, I am yet reminded of a quote by Mark Twain, "I have never let schooling interfere with my education", to which I still subscribe. TABLE OF CONTENTS CHAPTER 1. INTRODUCTION References CHAPTER 2. THE INFLUENCE OF SAMPLING INTENSITY, LIM[NG, P-RATE AND METHOD OF P APPLICATION ON P SOIL TEST VALUES Introduction Materials and Methods Results and Discussion Summary and Conclusions References CHAPTER 3. UPTAKE OF N, P, K, 5, Ca, Mg, Zn, Mn, Cu, AND B BY Cool-season GRASSES Introduction Materials and Methods Results Discussion Summary and Conclusions References 1 8 11 11 13 14 16 26 27 27 30 32 39 42 56 (HAPTER 4. EFFECT OF SOIL K ON TISSUE K IN PERENNIAL RYFGRASS AND TALL FESCUE Introduction Materials and Methods Results and Discussion Summary and Conclusions References 58 (I'HAPTER 5. SOLUTION P IN TWO SOILS AMENDED WITH LIME AND P Introduction Materials and Methods Results and Discussion Summary and Conclusions References 77 CHAPTER 6. FURTHER ACTION 94 BIBLIOGRAPHY 95 58 60 62 68 75 77 79 81 85 92 Page APPENDICES Appendix 1. Data from chapter 2 Appendix 2. Data from chapter 3 Appendix 3. Data from chapter 4 Appendix 4. Data from chapter 5 100 101 108 121 140 LIST OF FIGURES FIGURE Page 2.1. Method of random sampling. 16 2.2. Relationship between Bray-Kurtz and sampling error 17 with sampling intensity for the unlimed plots. 2.3. Effect of soil pH and sampling error by sampling intensity 18 for unlimed plots. 2.4. Relationship of Bray-Kurtz and Olsen-P with random and 19 systematic sampling for banded plots. 2.5. Relation of Bray-Kurtz and Olsen P with P application method. 20 2.6. Relationship of Bray-Kurtz and Olsen P with P rate. 21 2.7. Relationship between Bray-Kurtz and sampling error with 22 sampling intensity for limed plots. 2 8. Effect of liming on Olsen and Bray-Kurtz levels. 23 2 9. Correlation of Olsen with Bray-Kurtz. 24 3.1. Dry matter accumulation for five grass species by heat unit. 43 3.2. Normalized uptake of N, P, K and S for Fawn, tall fescue by heat unit. 44 3.3. Normalized uptake of N, P, K and S for Rebel, tall fescue by heat unit. 45 3.4. Normalized uptake of N, P, K and S for Newport, Kentucky bluegrass by heat unit. 46 3.5. Normalized uptake of N, P, K and S for Penniawn. fine fescue by heat unit. 47 3.6. Normalized uptake of N, P, K and S for Linn, perennial ryegrass by heat unit. 48 3.7. Normalized uptake of N, P, K and S for Pennfine, perennial ryegrass by 49 heat unit. pg 3.8. Normalized uptake of N, P, K and S for Potomac, orchardgrass by heat unit. 50 4.1. Effect of soil K on straw and seed K for tall fescue and perennial ryegrass. 69 4.2. Predicted straw K for tall fescue and perennial ryegrass from soil K. 70 4.3. Rate of change for straw K for tall fescue and perennial ryegrass 71 due to soil K. 4.4. Effect of soil K on straw K uptake for perennial ryegrass and tall fescue. 72 5.1. Effect of soil pH on Zn, Mn, Cu, Fe, and Al concentrations in 86 soil solution at Hyslop. 5.2. Effect of lime application on Soil test P, TP and P in soil solution 87 and P in soil solution 88 at Hyslop and Saddle Butte. 5.3. Effect of P application on soil test P, TP, at Hyslop and Saddle Butte. LIST OF TABLES Page 2.1. Coefficients of variation for lime rates and P rates at 0-15 and 0-2.5 cm depths. 3.1 Regression model of dry matter accumulation by heat units for five grass species by heat unit. 25 51 3.2 Dry matter production and nutrient content of five grass species by heat unit. 52 3.3 Uptake of N, P, K, S, Ca, and Mg at physiological maturity for five 55 grass species. 4.1 Seed yield and straw yield three year averages for tall fescue and perennial ryegrass by K application, residue management and K fertility. 4.2 Logistic growth model parameters for foliar K response of TF and PR to soil K. 73 74 Correlation of foliar K in TF and PR with Ca and Mg. 74 Analysis of variance for the effect of lime and P application on select P availability parameters at Saddle Butte and Hyslop sites. 89 5.2 Soil and solution pH at Saddle Butte and Hyslop 90 .3 I as effected by lime treatments. 5.3 Correlation coefficients for metals in soil solution vs soil solution and 1:2 pH for Hyslop and Saddle Butte sites. 91 NUTRIENT MANAGEMENT AND CYCLING IN GRASS SEED CROPS CHAPTER 1 INTRODUCTION Seed production is important to Oregon's agricultural economy in both number of acres and dollar value. Grass and legume seed crops occupied 500,000 acres in Oregon in 1993 with gross sales of over $200,000,000 (Miles, 1993). Grass and legume seed production accounts for seven percent of Oregon's agricultural commodity sales. Grass seed is produced throughout Oregon. Kentucky bluegrass is predominant in Eastern and Southwestern Oregon while the Willamette Valley produces primarily perennial ryegrass, annual ryegrass and tall fescue. Cool wet springs and poorly drained soils make the Willamette valley ideal for grass seed production. Grass seed crops are also well suited to the Willamette Valley's better drained soils but must compete for acreage with a wide variety of vegetable, nursery, fruit and grain crops. )uring 1993, 368,630 acres of grass seed were grown in the Willamette valley iiaking it the primary seed producing region in the state (Young, 1994b). Perennial ryegrass, annual ryegrass and tall fescue each ranked in Oregon's top twenty agricultural commodity gross dollars sales in 1993. Perennial ryegrass and tall fescue account for 43% of the grass and legume seed acreage in 1993 and 50% of gross sales dollars (Miles, 1994). These two grasses are primarily grown in Benton, Lane, Linn, Marion, Polk and Yamhill counties. Linn county produces more than three times the tall fescue and perennial ryegrass seed than any other county. Linn County's dominance of grass seed acreage results from the large area of poorly drained soils that occupy its western portion. Combined with poorly drained soils is a climate conducive to grass seed production. Winters are cool with high temperatures between 40° and 50° F and low temperatures between 20° and 30°. Precipitation exceeds transpiration from November through March. Soils are at or near saturation during winter and early spring. Above ground grass growth during winter is minimal because temperatures are low. 7 Spring in the Willamette Valley brings a gradual warming with a corresponding increase in grass growth. During late April and May. cool-season grasses flower, switching from vegetative to reproductive growth. Flowering may continue into June depending on temperature and precipitation. Grass seed harvest occurs in late June and July. Once harvest occurs, cool-season grasses become dormant because little moisture is available for growth. They remain dormant through the dry warm months of July, August, September and October. Fall precipitation initiates limited amounts of grass growth but cool temperatures prevent significant amounts of growth. During fall, late winter and early spring, cool-season root growth occurs depending on soil temperature. Cell division in root tips of cool-season grasses has been found at temperatures as low as zero degrees centigrade (Stucky, 1941), indicating roots are capable of growth during winter. Depending on year, active root number where cool-season grasses are grown for turf, peaks in May or June then decreases through summer with a secondary peak in late October or early November Koski, 1983). Similar patterns would be expected in grass seed fields since water is vailable and optimal temperatures exist during the spring and late fall. Root growth easona1it\ in cool-season grasses is needed to understand nutrient uptake. Total root number in the top 10 cm of soil for tall fescue and perennial ryegrass varies between 20% and 90% and is dependent on stand age, season and method of measurement (Koski, 1983). Most researchers find 50-80% of total root length in the top 10 cm of soil and this varies seasonally (Garwood, 1967: Wilkinson and Mays, 1979: Garwood and Sinclair, 1979). Roots initiated in the fall or winter remain active for 4-10 months while those formed in the summer remain active for one to three (Garwood, 1967: Parson and Robson, 1981). The large amount of roots in the top six inch for perennial ryegrass and tall fescue may help explain the effectiveness of 0-6 inch soil samples. Root to shoot ratios (R:S) have been shown to have higher in tall fescue than perennial ryegrass (Davidson, 1969). Tall fescue has also been shown to have higher dry matter yields than perennial ryegrass when these grasses are grown for seed (Horneck et al., 1988). Tall fescue will produce between 8.000 and 20.000 lb a1 where as perennial ryegrass will produce 4,000 to 10,000 lb a1. Greater dry matter production and higher R:S, indicates a more extensive root system may exist for fescue. Differences in dry matter production and root mass between grass species, has implications for straw management and nutrient uptake. Tillering and fertile tiller number are integral to high seed yields. Fertile tiller number controls seed yield barring late season deficiencies or drought (Young, 1988). Residue management effects fertile tiller number not seed weight or seed number per tiller (Stanwood, 1974: Smoliak and Johnston, 1968). Tillering in cool-season grasses can be controlled by light intensity (Mitchell, 1953: Troughton, 1960: Robson, 1972: Spirtz et al., 1972: Stanwood, 1974: Parsons and Robson, 1981: Ensigh, 1983). l)ecreases in fertile tiller number, due to shading from straw, was thought to be the reason for lower seed yields when residues are not burned after seed harvest Stanwood, 1974). Traditionally after seed harvest straw left on the field was burned. This process is termed "open field burning". Open field burning was used to dispose of straw, recycle selective nutrients, control volunteer seedlings, manage crown size and control disease. Burning was economical and easy for a grass seed grower to perform. Burning residues recycled nutrients such as K, Ca, Mg and P. Other nutrients such as N and S were volatilized and lost from the field. Alternatives such as baling straw, propane burning and composting had been considered economically unfeasible to grass seed growers. During the late 1980's and early 1990's, a change occurred from open field burning to systems that removed straw. Straw disposal method changes were precipitated by complaints about smoke and a smoke caused multiple fatality accident on I-S. This accident resulted in a temporary moratorium on open field burning and caused the legislature to limit the maximum acres that could be annually burned. Since 1985, the number of acres open field burned in the Willamette Valley has 4 decreased from 214,787 to 73,075 (Young, 1994a). Since 1987, grass seed acres in the Willamette valley have increased 21% (Young, 1994b). The transition from open field burning to straw management systems where residue is harvested occurred over the last five years. Once straw is removed after seed harvest the general practice is to "crew cut" or flail chop standing residues as close to the soil's surface as possible then vacuum sweep the field. Several variations have been tried. Some growers, instead of crew cutting and vacuum sweeping, propane burned their fields while others tried urea-sulfuric acid attempting to eliminate residues on the field. Some growers crew cut and leave the remaining residue on the field. Harvesting straw depletes the soil nutrient supply, especially K, because large amounts of high K content residue are annually removed. Residue management also effects surface rooting. Root mass has been shown to decrease in only the surface 2.5 cm depth where residue has been harvested as compared to open field burning Stanwood, 1974). Consequences rooting changes have on fertile tiller number, seed ield and nutrient uptake is unclear. Soil temperature and moisture differences may exist between burned and fields where straw is mechanically removed. Field studies have been conducted on native prairies demonstrating burning's effect on temperature. Soil temperature differences of one to five degrees centigrade for burned vs clipped plots have been measured (Hulbert, 1968). Burned plots were higher in soil temperatures throughout the growing season but as the season progressed differences narrowed. Soil moisture effects due to differences in straw management are currently being investigated in Oregon (Chastain and Young, 1994). Differences in soil temperature and moisture due to residue management may influence grass growth. Current trends in residue management for tall fescue and perennial ryegrass production are to finely chop back the entire straw load and let it decompose on the soil surface. A flail chopper is used to grind straw finely enough so that it will settle to the soil surface. Chopping back straw and leaving the residue is similar to open field burning from a nutrient cycling stand point. K is recycled, nutrients such as N and S which were volatilized in a burn are also recycled. Straw readily decomposes and releases nutrients prior to next years crop demand. Because nutrients are recycled, soil depletion and luxury consumption are of minimal concern unless seed ield is affected. Recycling of nutrients by open field burning in the Willamette Valley minimized the need for grass seed fertilization for nutrients other than N and S. Nutrients such as N and S are mobile in soil and able to leach as well as being volatilized when straw is burned, thus they require annual application. Elements such as P, Ca and Mg were recycled in an open field burn and are required by cool-season grasses in small amounts, thus fertilization need was small. Potassium was also recycled when grass seed fields were burned, but because of the large amounts of K used by cool-season grasses it is an element that is continually applied. Fertilization with sulphur, phosphorous and potassium for fine fescue, tall I cscue, Kentucky bluegrass, orchardgrass and perennial ryegrass has historically been eated similarly by Oregon State University and the grass seed growing community. I ertilizer guides (FG) 6 (Doerge et al., 1982), 36 (Doerge et al.,1983), 44 (Doerge et al.. 1982b). 45 (Doerge, et al., 1982c), and 46 (Doerge et al., 1982d) for these grasses have identical critical levels for P and K. The critical level for P is 25 ppm and 100 ppm for K. Similar critical levels for P and K were also used in pasture fertilizer guides: FG 16 (Gardner et al., 1984a) for grass pastures, FG 1 (Gardener et al., 1984b) for white clover-grass pastures and FG 4 (Jackson et al., 1983) for subclovergrass pastures. Critical soil nutrient levels for grass seed crops were established with no published research and based on a system which burned straw. Thus, they do not account for differences between grass species in dry matter production or straw K content. Systems that harvest straw must economically and environmentally balance nutrient removal rates, soil test levels, fertilization and seed yield. In contrast to fertilizer recommendations, soil pH recommendations by grass species are made in OSU fertilizer guides. Kentucky bluegrass and orchardgrass 6 guides recommend a soil critical level pH of 6.0 where as the tall fescue and perennial ryegrass guides recommend a critical soil pH of 5.5. Generally, OSU recommends a critical soil pH above the point where yields will be negatively affected. Lime is either top dressed to an established field or incorporated prior to a new seeding. Lime incorporation at seeding is preferred to later surface applications. Surface lime applications are assumed to be less effective than incorporation because of lower maximum recommended rates (2 t a1 vs 5 t a') and lime's immobility in soil. A surface lime application will effect soil pH in only the top one to two cm. Little soil fertility research had been conducted for grass seed crops until a nutrient status survey of 77 fields in the Willamette valley was completed in 1987 (1-Torneck et al., 1988). The survey precipitated questions regarding grass nutrition. The primary questions dealt with soil pH, P and K. Soil pH in the survey was lower than University guidelines while P and K levels were substantially higher. Soil P lcvels in the top six inch averaged 80 ppm where K levels averaged 300 ppm and i1 pH 5.2. Soil P and K levels were higher in the surface inch and pH was lower. ugh soil test P and K levels in grass seed fields where high were puzzling, since no :ocumentcd P deficiency exists for Willamette Valley grass seed fields. Explanations br high soil test levels such as "substitution of P for lime" was given by fieldmen and growers. "University recommendations are too conservative" was another reason given for high soil test levels. Soil test results should be the basis for lime, P and K fertilizer recommendations. High P levels found in the soil survey and the higher P concentrations in the surface inch compared to lower depths, raised the question whether a traditional six inch or a surface one inch soil sample was appropriate to asses fertilizer needs. Nutrient stratification could be effecting availability considering possible root restrictions due to poor drainage. Soil sampling theory is known for "the more subsamples that are combined the more consistent soil test results are." Increases in sampling intensity should therefore decrease sample variability. Fertilizer placement such as banding should theoretically increase soil variability making a 7 representative sample more difficult to obtain. The effect fertilizer and lime additions has on increasing soil test variability in grass seed fields is unknown. Cool-season grasses in the Willamette Valley could be more responsive to fertilizer than OSU fertilizer guides predicted. Soil sampling problem as to not getting reliable soil test results or not representing current nutrient distribution may be influencing a soil tests effectiveness. Simultaneously to extension questions regarding soil nutrient levels, growers asked 'what happens when we harvest straw instead of burning?" Grass species and varieties differences regarding uptake and consequent removal of plant nutrients needs to be documented. The amount of N, P, S and K utilized by grass seed crops was of immediate concern. These questions resulted in our interest to document grass nutrient uptake. Perennial crop growth is complex. Temperature, plant kinetics, plant age, environmental conditions and crop management all effect growth. When yield is affected crop uptake and removal are likely to follow. Fertilization practices should be adjusted based on soil tests to maximize yield yet minimize environmental effect. The objectives of this research were to determine nutrient uptake of five cooleason grass species. Differences between tall fescue and perennial ryegrass varieties as to when nutrient uptake occurs and quantity needed were also to be investigated. In an attempt to answer questions, generated from the grass seed survey regarding high soil test levels, two studies were designed to answer, how tall fescue and perennial ryegrass respond to lime, P and K. The effect liming, P placement and P rate has on P and pH soil test variability would be measured. References Chastain, I. and W. Young, 1994. Winter seminar. Davidson, R.L. 1969. Effect of root/leaf temperature differentials on root/shoot ratios in some pasture grasses and clover. Ann. Bot 33:561-569. I)oerge, TA., H. Gardner and T.L. Jackson. 1982b. FG 44: Blue grass seed. Oregon State Univ. Extn. Ser. Corvallis, Oregon. l)oerge, l.A., H. Gardner and T.L. Jackson. 1982. FG 6: Fine fescue seed. Oregon State Univ. Extn. Ser. Corvallis, Oregon. Doerge, T.A., H. Gardner, T.L. Jackson and H. Youngberg. 1982c. FG 45: Orchard grass seed. Oregon State Univ. Extn. Ser. Corvallis, Oregon. [)oerge, l.A., H. Gardner, T.L. Jackson, and H. Youngberg. 1982d. FG 46: Perennial ryegrass. Oregon State Univ. Extn. Ser. Corvallis, Oregon. )oerge, T.A., H. Gardner and H. Youngberg. 1983. FG 36: Tall fescue seed. Oregon State Univ. Extn. Ser. Corvallis, Oregon. nsign, RD., V.D. Hickey and M.D. Bernardo. 1983. Seed yield of Kentucky bluegrass as affected by post-harvest residue removal. Agron. J. 75:549-551. Gardner, H., T.L. Jackson and W.S. McGuire. 1984a. FG 16: Perennial grass pastures. Oregon State Univ. Extn. Ser. Corvallis, Oregon. Gardner, H., T.L. Jackson and W.S. McGuire. 1984b. FG 1: Irrigated clover-grass pastures. Oregon State Univ. Extn. Ser. Corvallis, Oregon. Garwood, E.A. 1967. Seasonal variation in appearance and growth of grass roots J. Br. Grassl. Soc. 22:121-130. . Garwood, E.A. and J. Sinclair. 1979. Use of water by 6 grass species. 2. Root distribution and use of soil water. J. Agric. Sci. 93:25-35. Horneck, D.A., and J.M. Hart. 1988. A survey of nutrient uptake and soil test values in perennial ryegrass and turf type tall fescue fields in the Willamette Valley. In: H.W. Youngberg (ed.). 1988 Seed Production Research. Dept. of Crop Science, EXT/CrS 74. p. 13-14. Oregon State University, Corvallis, OR. Hulbert, L.C. 1969. Fire and litter effects in undisturbed bluestem prairie in Kansas. Ecol. 50:874-878. Jackson, T.L., E.H. Gardner, W.S. McGuire and T.E. Bedell. 1983. FG 4: Subclover-. grass pasture. Oregon State Univ. Extn. Ser. Corvallis, Oregon. Koski, A.J. 1983. Seasonal Rooting Characteristics of five cool season grasses. M.S. Thesis Ohio St. Univ. Miles, S.D., 1994. 1993 Oregon county and state agricultural estimates. Special report 790. Oregon State Univ. Extn. Ser. Corvallis OR. Mitchell, K.J. 1953. Influence of light and temperature on growth of ryegrass (Lolium spp.). 1. Pattern of vegetative development. Physiologia Plantarum. 6:21-46 Parsons, A.J. and M. Robson. 1981. Seasonal changes in the physiology of S24 Perennial ryegrass (Lolium perenne L). 3. Partition of assimilates between root and shoot during the transition from vegetative to reproductive growth. Ann. Bot. 48:733-744. Robson, M.J. 1972. The effect of temperature on the growth of S.170 fescue (Festuca arundinacea). J. Appi. Ecol. 9:643-653. 'heffer, K.M. and J.K. Dunn. 1981. Anatomy, drought survival and rooting depth of cool season turf grasses. Agron. Abstr. p. 128. Smoliak, S. and A. Johnston. 1968. Germination and early growth of grasses at four root-zone temperatures. Can. J. P1. Sci. 48:119-127. Spiertz, J.H.J. and J. Ellen. 1972. The effect of light intensity on some morphological aspects of the crop perennial ryegrass (Lolium perenne L. var. "Cropper") and its effect on seed production. Neth. J. Agric. Sci. 20:232-246. Stanwood, P.C. 1974. Influence of post-harvest management practices on plant growth and seed yield of cool season grasses. M.S. Thesis. Oregon St. Univ. Stuckey, I.H. 1941. Seasonal growth of grass roots. Am. J. Bot. 28:486-491. Troughton, A. 1960. Further studies on the relationship between shoot and root systems of grasses. J. Br. Grassl. Soc. 15:41-47. Wilkenson, S.R. and D.A. Mays. 1979. Mineral Nutrition. pp. 41-74. In R.C. Buckner and L.P. Bush (ed.) Tall Fescue. No. 20. Am. Soc. Agron. Madison, WI. I0 Young, B. 1988. Personnel communication. Oregon State Univ. Young, W.C. 1994a. Willamette Valley grass seed production and burning data. 1986-93. Personnel communication. Oregon State Univ. Young, B. 1994b. Seed production. Iii Crop and soil news/notes. 8:2. pp. 6-8. Oregon State Univ. CHAPTER 2 THE INFLUENCE OF SAMPLING INTENSITY, LIMING, P-RATE AND METHOD OF P APPLICATION ON P SOIL TEST VALUES Introduction The variability of soil testing results can be assigned to three sources: (1) sampling error; (2) selection error; and (3) analytical error. Analytical error has been lound to range from 5 to 10% (Graham, 1959). Selection error is the error that may incur during sample processing such as grinding and drying. Selection error may or may not be minimal depending on soil type and the processing methods used. Sampling errors in soil testing have been found to be of a much greater magnitude than analytical errors associated with laboratory procedures (Cline, 1944). With improvement of analytical techniques and instrumentation, the difference between sampling and analytical errors has hypothetically increased since 1944, because ampling techniques have remained relatively unchanged. A decision must be made by researchers and growers about the time and cost f sampling compared to accuracy when deciding upon sampling intensity. A random sampling pattern is typically used in field research plots, whereas growers' fields are usually sampled systematically. Sampling within soil types and across large areas with random, systematic and composite samples has been reviewed previously by several authors (Petersen and Calvin, 1982; Sabbe and Marx, 1987). A systematic, zig-zag pattern produces more reliable results than a random pattern (Petersen and Calvin, 1982; Sabbe and Marx, 1987). Management, erosion and number of soil types within a field are of much greater importance than field size in contributing to sampling error (Graham, 1959). Kunkel et al.. (1971) sampled 24 small uniform fields between 0.093 and 1.26 hectares in size and found coefficients of variation of 20 to 40% for P soil test levels within a field. These results indicated the importance of soil heterogeneity on experimental data, and the possible invalidation of results due to block by treatment interactions. 12 Addition of soil amendments and fertilizers increases soil heterogeneity, especially where fertilizers have been banded. Sampling intensities must be adjusted to account for this increased variation. Hooker (1976) found that 7 and 104 subsamples were required to obtain a representative sample with equal deviations where P was broadcast and banded, respectively. Reed and Rigney (1947) analyzed a uniform soil for P and determined that four samples were necessary to obtain accuracy within ± 6 mg P/kg soil. On a nonuniform soil, 19 cores were necessary for the same accuracy. To estimate pH, 7 cores for uniform soil and 18 for nonuniform soil were necessary to be within 0.1 and 0.2 pH units respectively. Kunkel et al.. (1971) confirmed that a large number of subsamples are needed to obtain results within 25% of the mean, even on small fields. They also determined that more samples were needed to obtain a representative sample for soil P than for K. Soil sampling is an integral part of soil testing. The heterogeneity of soils and a samples' failure to represent field conditions can render analytical results useless. A 'epresentative soil sample is therefore a prerequisite for meaningful analytical results Lnd an accurate fertilizer recommendation. The objectives of this study were to: (1) measure sampling variation for Bray-Pl, Olsen P and pH at four sampling intensities; (2) determine the influence of lime and P additions on sampling error (3) measure changes in soil test P with altered PH; and (4) compare Bray-P 1 and Olsen P extractants for detecting P additions. 13 Materials and Methods The experiment was initiated in the fall of 1988 near Shedd, Oregon. The experimental site was on a Bashaw clay soil (typic pelloxerert) with a Bray-P 1 of 8 mg/kg and a 2:1 water:soil pH of 5.2. A split plot design was used with three replications in a randomized complete block arrangement. The main plots were limed with 0 and 4.5 t CaCO3 equiv./ha. Subplots were rates of 0, 26.5, 53 and 79.5 kg P/ha applied as either a surface band or broadcast. Subplot size was 9.1 m by 2.4 m. Lime was applied and incorporated in the fall of 1988 prior to planting. Perennial ryegrass (Lolium perenne) was seeded in 30 cm rows. Carbon was surface banded over the rows at planting and the field was treated with 2.7 kg ai diuronlha. After planting P was either surface broadcast or banded using phosphoric acid. For soil sampling, each plot was overlaid with a 6.1 m by 2.4 m rectangle, with lines every 30.4 cm using PVC pipe and nylon twine (Figure 2.1). Random ample points were permanently marked for each sampling intensity so that the same pot was sampled for each plot. Four sampling intensities (5, 10, 20 and 40 cores per ample) ere used for the 0-15 cm depth. A 0-2.5 cm sample was also taken at the 40 core intensity. Banded plots were also sampled systematically at the 40 core intensity with 50% of the subsamples taken within the band. Soil samples were air dried and ground to pass a 2 mm sieve. Each sample was analyzed for pH (2:1, water:soil), Bray P-i and Olsen P (colormetrically) as described by Horneck et al. (1989). Means and standard deviations were calculated for each treatment over three replications to examine variability. Data was subjected to analysis of variance to differentiate between treatments. 14 Results and Discussion Increasing sampling intensity from 5 to 40 random samples did not decrease sampling error. P means or sampling errors by either Bray (Figure 2.2) or Olsen (data not shown) extractants did not differ significantly between sampling intensities. Soil pH means and sampling errors also did not change with sampling intensity (Figure 2.3). This is in contrast to previous work which has shown that sample variation decreases as the number of subsamples is increased (Kunkel et al., 1971; Hooker, 1976). Breaking down the data in the current study into individual treatments showed no trend toward decreased sampling error with increased sampling intensity. These results suggest that a soil sample with 5 subsamples represented field-plot conditions as well as one that contained 40. The number of samples and number of cores per sample needed to represent a small field plot within a single soil type may not be important if plots are sampled in an identical random pattern. Since no difference 'xisted between sampling intensities, Figures 2.4, 2.5, 2.7, and 2.8 contain data from he 40 subsample intensity only. Banding P has been shown to increase soil variability and result in higher sampling errors than where P was broadcast (Hooker, 1976). This experiment showed soil test P means and variability between broadcast and banded plots to be similar using either the Bray-P 1 or Olsen extractants (Figure 2.5, LSD 0.05, Bray=l.7, Olsen=3.6). A systematic sampling procedure, where one half the sample is obtained within the fertilizer band, would increase the number of samples taken in space where P had been applied, and thus was expected to increase soil test P values over a random sample. No differences were observed between the random and systematic samplings (Figure 2.4, LSD 0.05, Bray=1 .9, Olsen=4.0). The lack of differences between band and broadcast and sampling method could be the result of a large soil P buffering capacity and soil P fixation. However, since P fertilization increased extractable P (Figure 2.6, LSD 0.05, Bray=2.4, Olsen=5.0), this should not be the 15 case. Possibly, the differences were hidden in the soil's natural variability and not detectable after one P application. Liming significantly increased pH to 6.4 and significantly decreased Olsen soil test P (Figure 2.8, LSD 0.05, Bray=17, Olsen=3.6). The same trend was apparent for Bray P but the differences were not significant. The addition of lime (Figures 2.2, 2.7) or P (Figure 2.6) did not increase soil P heterogeneity, as indicated by uniform and randomness of sampling error bars for Bray P (Figures 2.2, 2.6, 2.7) and Olsen P (Figure 2.7). Data from Olsen P is not all shown because it behaved the similarly as Bray P. Liming has been shown to produce a more favorable microbial environment. resulting in increased immobilization of P, perhaps accounting for the lower soil test P when lime was applied (Dahl, 1977). Olsen P and Bray P were highly correlated, with Bray P being approximately one half of Olsen P for all samples (Figure 2.9). This is probably due to the high pH (8.5) of the Olsen extracting solution and the resultant dissolution of iron and aluminum phosphates which may then precipitate as hydroxides (Olsen and Sommers. I 982). The possibility also exists for ligand exchange with the hydroxyl ion and nteractions with organic phosphates. Coefficients of variation (CV) averaged 3% and 24% for pH and P, respectively (Table 2.1). Analytical errors calculated from soil standards analyzed with the samples were 1.1% and 5-7% for pH and P, respectively. By subtracting analytical errors from total error, an estimate of sampling error can be determined. This results in approximately 2% and 20% error due to sampling for pH and P. respectively. No discernible trend in CV was evident for P rates and lime treatments. Any increase in variation due to P rate (Figure 2.6) was not observed in CV. Sample variation for the 0-2.5 cm depth tended to be lower than the 0-15 cm for soil P, but higher for pH. 16 Summary and Conclusions Liming and P application had no effect on sampling errors after a single application. Liming significantly decreased Olsen P. with Bray P showing the same trend. Soil test P increased with P rate for both Olsen and Bray soil tests. Olsen P was highly correlated and twice Bray P (r=0.8). Increasing sampling intensity from 5 to 40 subsamples did not decrease sampling error. Based on this study a soil that is by appearances relatively heterogeneous within a single soil type can be sampled within small areas with low sampling intensities. 2.4 m PVC PIPE Figure 2.1. Method of random sampling. 17 mg/kg 30 20 10 0 10 20 40 50/50 SAMPLING INTENSITY (SAMPLES/PLOT) Figure 2.2. Relationship between Bray-Kurtz and sampling error with sampling intensity for the unlimed plots. (Error bars represent one standard deviation) 18 SOIL pH .1 / 5 10 20 40 50/50 SAMPLING INTENSITY (SAMPLES/PLOT) Figure 2.3. Effect of soil pH and sampling error by sampling intensity for unlimed plots. (Error bars represent one standard deviation) 1') mg/kg 40 0 BRAY-KURTZ OLSEN P 30 20 I'll 0 RANDOM 50/50 Figure 2.4. Relationship of Bray-Kurtz and Olsen P with random and systematic sampling for banded plots. (Error bars represent one standard deviation) 20 mg/kg 40 BRAY-KURTZ OLSEN P 30 20 10 0 BAND BROADCAST Figure 2.5. Relation of Bray-Kurtz and Olsen P with P application method. (Error bars represent one standard deviation) 21 mg/kg 40 BRAY-KURTZ OLSEN P 30 20 10 0 26.5 53 79.3 P APPLIED kg/ha Fig 2.6. Relation of Bray-Kurtz and Olsen P with P rate. (Error bars represent one standard deviation) 22 my/kg 30 20 10 5 10 20 40 50/50 SAMPLING INTENSITY (SUBSAMPLE/SAMPLE) Figure 2.7. Relationship between Bray-Kurtz and sampling error with sampling intensity for limed plots. (Error bars represent one standard deviation) mg/kg 40 BRAY-KURTZ OLSEN P 30 20 10 0 UNLIMED LIMED Figure 2.8. Effect of liming on Olsen and Bray-Kurtz levels. (Error bars represent one standard deviation) 24 mg/kg 50 40 00 00 000 r=0.80** 00 30 00 0 006o8 osoo 0 20 0 0 0 0 0 9 0 0 00 0 10 0 20 BRAV-KURTZ (mg/kg) 10 Fig 2.9. Correlation of Olsen with Bray-Kurtz. **significt at 0.1 level. 30 "S TABLE 2.1 Coefficients of variation for lime rates and P rates at 0-15 and 0-2.5 cm depths. *L± LIMED **LO UNLIMED BRAY-KURTZ pH P Rate L0** L+* L+ LO kg/ha ........................ % OLSEN L+ LO ....................... 0-15 cm DEPTH 0 2 3 37 36 19 23 26.5 3 3 17 32 5 21 53 3 4 33 27 28 19 79.3 3 3 32 28 37 34 2.8 3.3 29.8 30.8 22.3 24.3 average 0-2.5 cm DEPTH 0 1 6 29 22 20 14 26.5 3 3 25 25 12 23 53 4 5 24 22 33 13 79.3 4 3 19 19 21 27 average 3 4.3 24 22 21.5 19.3 STANDARDS: LAST 6 MONTHS 1.5 7.3 10.9 WITHIN ANALYSIS 1.1 5.1 7.5 26 References Cline, M. G. 1944. Principles of soil sampling. Soil Sci. 58:275-288. Dahi, R. C. 1977. Soil organic phosphorous. Jn: N. C. Brady (ed). Advances in Agronomy. 29:83-117. International Rice Institute. Manilla, Philippines. Graham. E. R. 1959. Soil Testing. Univ. Mo. Agric. Exp. Sta. Bull. No. 734. Hooker, M. L. 1976. Sampling intensities required to estimate available N and P in five Nebraska soil types. Univ. Ne. M.S. Thesis. Horneck, D. A., J. M. Hart, K. Topper, and B. Koepsell. 1989. Methods of soil analysis used in the soil testing laboratory at Oregon State University. In Press. Kunkel, R., C. D. Moore, T. S. Russell, and N. Holstad. 1971. Soil heterogeneity and potato fertilizer recommendations. Amer. Pot. J. 48:163-173. Petersen, R. G. and L. D. Calvin. 1986. Sampling. hi: A. Klute. (ed.) Methods of soil analysis, 2nd edn. Part 1, pp. 33-52. Amer. Soc. of Agron, Madison, WI. eed, J. F. and J. A. Rigney. 1947. Soil sampling from fields of uniform and nonuniform appearance and soil types. J. Amer. Soc. Agron. 39:26-40. Sabbe, W. E. and D. B. Marx. 1987. Soil sampling: spatial and temporal variability. in: J. R. Brown (ed.) Soil testing: sampling, correlation, and interpretation, pp. 1-14. Soil Sci. Soc. Amer. Madison, WI. 27 CHAPTER 3 UPTAKE OF N, P, K, 5, Ca, Mg, Zn, Mn, Cu AND B BY Cool-season GRASSES Introduction Nutrient management for seed production of cool-season forage and turf grasses is constrained by the lack of reported research. The problem is compounded by the proliferation of new cultivars and changes in residue management that influence nutrient cycling. Fertilizer trials on a variety of soil types, grass species and residue management systems is not currently economically feasible. Economic, agronomic and environmentally sound nutrient management requires an understanding of differences in dry matter accumulation and nutrient concentration between cool-season grass varieties and species. Matching fertilization to crop need can be estimated by measuring crop uptake patterns. Fertilizer needs to be applied nrior to plant demand but close enough that losses to groundwater or erosion are ninimized. Once growth and uptake patterns are known species and cultivar specific iertilizer rates and timing can be approximated. Nutrient uptake for the annual grasses, wheat, and corn are well documented and used in extension and industry programs with producers. Producers of grass for seed and hay can use similar information for fertilizer timing in cool-season forage and turf grasses. The continued application of fertilizer when soil tests were three times Oregon State Extension recommendations for cool-season grasses added to the interest of documenting grass nutrient uptake through a growing season. Results from a survey of 77 tall fescue and perennial ryegrass fields in the Willamette Valley showed average soil test P to be 80 ppm and K to be 300 ppm (Horneck & Hart., 1988). The established critical level for P is 25 ppm and 100 ppm for K (Doerge et al.,1982a: Doerge et al.,1982b:Doerge et al.,1982c: Doerge et al.,1982d: Doerge et al.,1983). Previous work showed that phosphorous concentrations in tall fescue and perennial ryegrass tissue remain constant across soil test level making P uptake dependent on dry matter production instead of soil test level, or fertilizer applied (Horneck et al.,1992b). Cool-season grasses such as tall fescue and perennial ryegrass when grown to maturity, have low P concentrations. 0.1 to 0.2% (Kelling and Matocha. 1990). Concentrations tend to be lower where these grasses are grown in cooler environments (Gerwing et al., 1991: Horneck et al., 1992b) and more variable in warmer climates (Hillard et al., 1992). Potassium is used by grasses in variable amounts. Tall fescue straw at seed harvest will contain between 50 to 300 lb K a1 depending on dry matter produced. Potassium concentration at maturity will vary between 0.50% to 3.0% depending on soil test level (Horneck et al. ,1992a: Kuhlmann, 1990). Changes in K soil test level will effect plant K concentration as well the nutrition of other minerals such as Ca and Mg (Grunes et al., 1992: Orlovius, 1992). Nitrogen, P, K and S are nutrients most commonly applied to grass seed crops. Nitrogen should be managed to meet crop demand as well as safeguard ground water. I o insure efficient N use by plants, N must be applied close to peak demand at rates matching crop uptake. Sulphur and nitrogen, because of their mobility in soil, are upplied annually. Sulfur is less mobile in the soil and has a lower crop need than N. Sulfur also in not a current environmental concern which combine to make fertilizer timing less critical. Potassium and P are applied in the fall. The application of P and K should be based on soil tests. Rarely are cool-season grasses fertilized with Zn, Cu, Mn or B. Increasing fertilizer purity, high yields and continued cropping history may create deficiencies and a requirement for micronutrient fertilization in the future. Micronutrient concentrations in grass species may increase where industrial and municipal wastes have been applied to the soil. Metal concentrations reported here should provide reference concentrations at several points during the growing season. Objectives of this study are to compare dry matter accumulation and nutrient uptake differences at five sampling dates between five grass species. Differences in mineral uptake within tall fescue and perennial ryegrass species are also examined. 29 When nutrient uptake occurs relative to drymatter and how concentration changes over a growing season is important for making fertilizer decisions. 30 Materials and Methods Seven varieties, selected from a variety trial with four replications, were sampled during 1988 from initiation of spring growth to physiological maturity for seed production. The variety trial Was arranged in a random complete block design and had been harvested for two previous seasons. Varieties sampled were Linn and Pennfine perennial ryegrass (Lolium perenne); Fawn (pasture) and Rebel (turf-type) tall fescue (Festuca arundinacea); Newport Kentucky bluegrass (Poa pratensis); Potomac orchardgrass (Dactylis glomerata); and Pennlawn fine fescue (Festuca ruba). Residue management after the 1987 harvest included straw removal followed by propane flaming of the remaining stubble. Nitrogen was applied in the fall of 1987 across grass species at a rates of 32 lb a' and in the spring of 1988 prior to the February, 23 sampling at 110, 80 and 140 lb a' for perennial ryegrass plus fine lèscue, tall fescue plus orchardgrass and Kentucky bluegrass, respectively. Weed control for each individual grass species was performed to conventional standards. Whole plant samples were clipped from three feet of row from each of four replications, dried, ground and analyzed for kjeldahl N and P. A perchioric digest was analyzed by atomic absorption for K, Ca, Mg, Zn, Cu, Mn, colormetricaly with azomethine-H for B and turbidimetrically for S. A 0.5 g sample was used for analysis to decrease analysis sampling error. Growth of perennial ryegrass, and Kentucky bluegrass was insufficient to sample on February, 23. Data were analyzed in AN OVA as an unbalanced split-plot with the split being heat units. Linear models were calculated with SAS though a single intercept to determine dry matter accumulation differences between grasses (Engelstad, 1968). A single intercept linear model was used to compare dry matter accumulation rates between grasses. Differences between grass species and varieties within species was tested. Sigmoidal or individual linear models could be used to accomplish the same objective. The single intercept model was chosen for its simplicity. n Growing degree days, or heat units (HU) based on 0 degrees centigrade, from January 1, were measured and plotted on graph X axis to enable future comparison between years and growth stage. Heat units were calculated by taking the maximum and minimum air temperatures and dividing by two, then summing across days. The lowest air temperature used was 0 degrees centigrade and negative values were corrected to zero. Grass growth during Oregon winters is controlled by temperature. Cool-season grasses have been shown to grow at temperatures as low as 0 degrees centigrade. The use of heat units instead of days should yield better estimates of when fertilizers need to be applied in any given year. Heat units for harvest dates were 250 HU (February 23), 378 HU (March 12), 564 HU (April 2 ), 819 HU (April 27) and 1470 HU (June 17). Sampling dates were concentrated in the early growing season where fertilizers are more likely to be applied. Normalized uptake was calculated to visualize nutrient uptake relative to dry matter production or growth. Normalized uptake was calculated by taking uptake at ll the sampling dates and dividing by uptake at 1470 HU. The product is then multiplied by 100 to put results on a percent basis. Normalized uptake was then statistically analyzed in ANOVA at each date. Soil test levels from 0-6 inch for the plot area were pH ppm, K 144 ppm and Ca 5.0, Bray-Pi - 154 4.9 meq 100g'. Based on OSU fertilizer guides pH was below the 5.5 optimum for these grasses (Doerge et al., 1983), especially for Kentucky bluegrass (Doerge et al., 1982b) and orchardgrass (Doerge et al., 1982c) which recommend pH to exceed 6.0. Seed yield was not measured separately, but was included in June biomass harvest, 1470 HU. Previous years' seed yields for this site are reported by Youngberg and Young, 1988. Seed yields at this site in 1986 and 1987 are comparable to Western Oregon industry standards. 32 Results Grass growth, in this study followed a sigmoidal or exponential pattern depending on grass species when measured against time or days. Growth plotted against heat units yields linear response curves (Fig. 3.1). Kentucky bluegrass, between 250 - 1470 HU was the only grass to have a distinctly sigmoidal shaped growth response curve (Fig. 3.1). Dry matter leveled off between 819 and 1470 HU for Kentucky bluegrass, where the other six grasses maintained linearity through 1470 F-lU. Dry matter accumulation for the seven grasses initiates at approximately 200 HU (Fig. 3.1). Little dry matter accumulates between 200 and 400 HU. Differences in growth between grass species and varieties become apparent at 500 HU. Six of the grasses accumulated 40-50% of their total dry matter by 800 HU, Newport was the highest at 62% (Fig 3.1). The tall fescue varieties had the most growth and Kentucky bluegrass the least at all the harvest dates. Dry matter at the final harvest varied )etween 5427 lb a1 for Kentucky bluegrass and 15879 lb a for Fawn tall fescue. I'awn was significantly greater in dry matter than Rebel, the two tall fescue varieties, at 1470 HU, p=O.OS (Table 3.2). The two perennial ryegrass varieties were not different in dry matter production at 1470 HU, p=O.lO. Linn, perennial ryegrass however, averaged 1182 lb a1 more dry matter than turf type Pennfine. Dry matter production was variable between blocks with differences of 100% at the final sampling date. Variation at the early sampling dates between replications exceed 700%. Variability is caused by the irregular growth of cool-season grasses. They tend to grow in clumps, leaving areas within rows with either a high or low plant population. Average plant density does not exist. Dry matter accumulation was estimated with a model. A linear model with a common intercept was used to determine differences in growth rate between grasses (Table 3.1). Slope in the model is a measure of above ground dry matter accumulation rate. The seven grass varieties varied in dry matter accumulation rate on from 4.84 lb a' HU1 to 13.32 lb a1 HU1 and were divided into four groups. Kentucky bluegrass had the lowest dry matter accumulation rate, Fawn tall fescue the highest, Rebel Tall fescue the second highest and the remaining 4 grasses (Table 3.1). The model under estimated dry matter production for orchard grass, fine fescue and perennial ryegrass (Fig 3.1). Kentucky bluegrass and orchard grass had the poorest fit to the model. Both Kentucky bluegrass and orchard grass had R2=0.84 with the other three grass species ranging from R2=0.89 to 0.98 The sigmoidal growth curve of Kentucky bluegrass and orchardgrass resulted in a lower degree of fit to the linear model. The tall fescue varieties were significantly different in dry matter accumulation rate and both had higher dry matter accumulation rates than the other grasses (Table 3.1). The two perennial ryegrass varieties did not differ in dry matter accumulation rate at p=O.lO but were different at p=0.lS. Linn and Fawn, the forage varieties, had higher accumulation rates within species than the corresponding turf varieties pennfine and Rebel. Consistent trends between grasses and across sampling dates for N are difficult 10 discern. Nitrogen concentration tended to increase and decrease between sampling date with out much pattern (Table 3.2). Nitrogen concentration between 819 and 1470 HU was dependent on grass species. Nitrogen concentration remained constant between 819 and 1470 HU for tall fescue. During this period, N concentration in perennial ryegrass and orchardgrass decreased and increased in Kentucky bluegrass and fine fescue. At maturity, fine fescue had 3.05% N, which was greater than Kentucky bluegrass at 2.57% N, both had higher N concentrations at maturity than the other three grass species. Linn perennial ryegrass had a lower N concentration at 1470 HU than the other grasses. Nitrogen uptake, except fine fescue, follows the same ranking observed in dry matter production. Nitrogen uptake at 1470 HU is divided into four groups, Fawn tall fescue at 304 lb a, fine fescue and Rebel tall fescue averaging 256 lb orchardgrass at 203 lb a1 a1, and perennial ryegrass and Kentucky bluegrass which 34 averaged 142 lb a1 (Table 3.3). The exception to N uptake following dry matter is fine fescue which had 3.05% N at 1470 HU where the rest of the grasses averaged 1.83% N. The high N content of fine fescue makes it a moderate producer of dry matter at 8659 lb a1 and a high user of at N 265 lb a1 (Table 3.3). Average sulphur concentration fell from 0.3% to 0.14% over the growing eason (Table 3.2). Significant differences in S concentration between grasses exist at il1 sampling dates narrowing as grasses matured. Since S concentrations were similar between grasses at 1470 HU, S uptake was similar to dry matter production at 1470 1-lU (Table 3.3). Sulfur uptake at 1470 HU ranged from 22.3 for Fawn tall fescue to .6 lb a' for Kentucky bluegrass, the highest and lowest in dry matter production. respectively. Potassium concentration in all seven grasses increased during the period 250 through 819 HU then decreased during 819 to 1470 HU (Table 3.2). Concentration at 78 HU averaged 2.3% across grasses, 2.9% at 819 HU and 1.9% at 1470 HU. )rchard grass was consistently higher in K concentration across sampling dates. Linn perennial ryegrass was significantly lower in K than the other grasses at maturity. Potassium concentration within a species between the tall fescue and perennial ryegrass varieties were significantly, p=O.lO, different at 1470 HU (Table 3.2). Pennfine and Rebel, the turf type varieties, were higher in K concentration than the forage varieties Linn and Fawn, respectively. They were not consistently different throughout the growing season. Potassium uptake at the final harvest ranged from 121 lb a for Kentucky bluegrass to 295 lb a' for Fawn tall fescue (Table 3.3). Tall fescue and orchardgrass were higher in K uptake than perennial ryegrass fine fescue and Kentucky blue grass at 1470 HU. Within grass species, the tall fescue and perennial ryegrass varieties were not significantly different in K uptake. Higher dry matter levels by the forage varieties and higher K concentrations for the turf result in no difference in K uptake. Phosphorous, Mg and Ca concentration remained relatively constant or decreased gradually over growing season (Table 3.2). Perennial ryegrass had the 35 highest P concentrations of the seven grasses at 819 HU and the lowest at 1470 HU. Fine fescue and Kentucky bluegrass were higher in P concentration than the other three species at 1470 HU. The tall fescue varieties were not different from each other at 1470 HU. Phosphorous uptake at 1470 HU varied between 12.3 lb a' and 30.6 lb a for Linn and Fawn, respectively (Table 3.3). Fine fescue utilized 29.9 lb a* The two perennial ryegrass varieties had the lowest P uptake averaging 14.0 lb a1 and did not differ from each other. Phosphorous uptake for Linn perennial ryegrass at 12.3 lb a1 was significantly less than Kentucky bluegrass at 18.9 lb a'. Phosphorous uptake for Pennfine was less than Kentucky bluegrass but the difference was not significant (Table 3.3). Fawn tall fescue at 30.6 lb P a1 was significantly more than Rebel at 22.6 lb a* Calcium concentrations varied between sampling dates (Table 3.2). Few differences in Ca concentration were observed between grasses. Calcium uptake at I 470 HU was divided into a low group, Kentucky bluegrass and fine fescue averaging 5.2 lb a. and a high group consisting of the other three grass species, averaging 31.1 lb a' (Table 3.3). These Ca levels represent a ton of lime per acre every 15 and 30 years to replace the Ca removed in straw and seed for the high and low groups. respectively. Magnesium concentrations were consistent across samplings for each grass. Rebel tall fescue was higher in Mg concentration than the other six grasses at all sampling dates. Fawn tall fescue and Orchardgrass tended to be higher in Mg concentration than Kentucky bluegrass, fine fescue and perennial ryegrass. Both perennial ryegrass varieties had 0.11 % Mg at maturity. Magnesium uptake ranged from 23.0 lb a' for tall fescue to 5.56 lb a1 for Kentucky bluegrass. Tall fescue and perennial ryegrass did not differ within species. Magnesium uptake differences followed dry matter because of the consistency between grasses (Table 3.2). The micronutrients Zn, Cu, Mn and B as well as Ca, Mg, S and P tended to decrease in concentration with increased growth or heat units. Concentration 0 variability between grasses also tended to decrease with increased growth. Micronutrient concentration and uptake data is intended to provide reference for future work. Grasses are not currently being fertilized with micronutrients in Oregon except via bio-solids or manures. Zinc concentrations varied between 20-32 ppm in the early growing season. Early season (250-564 HU) Zn concentrations tended to increase or decrease between sampling without any pattern. However, as the season progressed to 1470 HU Zn levels became less variable and decreased to less than 15 ppm (Table 3.2). Six of the seven grasses contained between 0.1-0.2 lbs/a Zn at the final sampling. Kentucky bluegrass took up significantly less Zn, 0.07 lbs/a at 1470 HU, than the other grasses. Manganese concentrations varied between grass species throughout the growing season (Table 3.2). Manganese concentration decreased from 150-325 ppm to 100-225 ppm from 250 HU to 1470 HU as a result of early uptake then dilution in dry matter. Fine fescue and orchardgrass were consistently high in Mn concentration. Kentucky }luegrass did not demonstrate elevated Mn concentrations compared to the other rasses. Manganese uptake at 1470 HU was separated into 3 groups with orchardgrass the highest (2.5 lb ad), and Kentucky bluegrass the lowest (0.6 lb a1). The remaining erasses utilized between 1 and 2 lb a1 Mn. Copper concentration varied between 3 and 11 ppm for these grasses and in general decreased from an average 6 ppm to 3 ppm over the growing season (Table 3.2). Orchardgrass however, decreased from 11 ppm to 3 ppm. Copper concentrations remained constant between 819 and 1470 HU (Table 2). Because Cu concentrations between grasses were similar at 1470 HU differences, in Cu uptake are attributed to dry matter accumulation. Cu uptake by these grasses ranged from 0.02 to 0.05 lb a1. Boron concentration decreased through the first four sampling dates then increased (Table 3.2). Boron concentration varied between 1 and 11 ppm at 250 HU, 1-4 ppm at 519 HU and 6-9 ppm at 1470 HU. Tall fescue and Kentucky bluegrass tended to have the lowest B concentrations, but this was variable between sampling dates. The grasses were divided into three groups for B uptake with Kentucky o -, -'I bluegrass the lowest at 0.05 lb a' at 1470 HU. The mid-range group Rebel tall fescue, perennial ryegrass and fine fescue, varied between 0.07 to 0.09 lb a1. Fawn tall fescue and Orchardgrass had 0.11 and 0.1 lb B a' at 1470 HU, respectively. Normalization of data is a simple way to determine when nutrient uptake occurs relative to growth or dry matter production (Fig. 3.2-3.8). When Nutrient accumulation occurs relative to observed growth is important for making fertilizer decisions. Growth is the visual indicator that growers use to decide N, P, K and S fertilizer timing. Normalized uptake of the seven cool-season grasses studied is divided into two groups, grasses that accumulate nutrients in advance of drymatter and grasses where uptake and growth occurs in close proximity to each other. Nutrient uptake is species and nutrient specific. Grasses tended to accumulate P and K farther in advance of growth than N and S. Nutrient uptake and dry matter production closely coincided for Tall fescue Fig 3.2 and 3.3), Kentucky bluegrass (Fig. 3.4) and fine fescue (Fig. 3.5). \Jormalized uptake curves for these grasses were linear when compared to grasses that accumulate nutrients prior to growth. Nitrogen uptake rate decreased between 569 md 819 FlU then increased between 819 and 1470 HU. Nitrogen uptake also tended to lag behind dry matter in this group at 819 HU. Fine fescue for example had accumulated 50% of its dry matter at 819 HU, but only 31% N. Fine fescue had accumulated 29% N by 569 HU. Fawn tall fescue had accumulated 50% of both dry matter and N at 819 HU. Sulfur uptake closely coincides with dry matter for tall fescue (Fig 3.2 and 3.3). Sulfur uptake for fine fescue and Kentucky bluegrass precedes growth by 15%. Potassium uptake at 819 HU occurred 12% to 15% in advance of dry matter production. Fawn (Fig 3.2), the only pasture grass in this group, accumulated K 30% in advance of dry matter at 819 HU. Perennial ryegrass (Fig 3.6 and 3.7) and orchardgrass (Fig 3.8) are the grasses where nutrient uptake precedes growth or dry matter production. Normalized nutrient uptake curves are sigmoidal shaped compared to grasses where uptake and growth coincide. Uptake and drymatter occurs at the same rate prior to 400 HU. Uptake 0 precedes dry matter once 500 HU have been accumulated. Linn perennial ryegrass accumulated 38% of its drymatter at 819 HU but 50% S, 73% K, 95% N and 140% P. Phosphorous uptake precedes K, N and S. Sulphur in this group of grasses was the only nutrient which uptake coincided with dry matter. Discussion Tall fescue and perennial ryegrass varieties differ in their intended usage. Fawn and Linn are pasture varieties, where as Rebel and Pennfine are newer varieties bred for turf. The forage varieties, bred for dry matter yield, produced more dry matter than the turf varieties. Turf varieties tend to be higher in nutrient concentration than forage. The reasons for the inconsistent behavior of N concentration between grasses and sampling dates is unclear. Further research is needed to determine whether late N uptake in grasses like fine fescue and Kentucky bluegrass is necessary for current years seed yield or fall growth and next seasons seed. Late season N uptake may be related to fine fescue and Kentucky bluegrass producing stolens. Late season N uptake would coincide with spring N mineralization. Nitrogen concentration needs for seed yield are complicated by these grasses being bred for turf Turf color requires High N, whether this same level of N is needed to produce good seed yields in Llnknown. Late N use may be luxury consumption relative to seed yield. Higher nutrient concentration of turf varieties for elements such as N and K is observed through out this study. Differences in N concentration and uptake early in the growing season can be used in making N fertilizer rate and timing decisions. Perennial ryegrass and orchardgrass accumulated 60-90% of their N by 819 HU (Fig. 3.6 to 3.8). In contrast, the remaining grasses accumulated 3 1-52% (Fig. 3.2 to 3.5) of their total N by 819 HU. Nitrogen fertilization for perennial ryegrass and orchardgrass production should occur in the spring prior to 819 HU. Nitrogen applications for the other grass species studied can be delayed if late season N is needed for seed production. The early N uptake of perennial ryegrass makes timing of split N applications difficult because 80% of its N has been taken up prior to 819 HU. The other grasses studied would require 40-50% N application at 200 to 300 HU with the rest applied at or near 819 40 HU. During Oregon's wet winter months N applications need to be closely matched to grass demand to maximize N uptake, fertilizer efficiency and minimize N losses. These grasses tend to be good N scavengers, evidenced by N-rates for tall fescue totaling 122 lb a' and N uptake averaging 275 lb a1. Nitrogen rates are difficult to recommend from this study since there is an incomplete understanding on N required for seed yield compared to N needed to maintain a green field. The reason potassium concentration increases over much of the growing season ibilowed by a decrease after 819 HU is unclear. A possible explanation is that during later growth stages K is being diluted in dry matter. In addition, the dilution may be compounded by surface K being unavailable due to soil moisture depletion since soil tests indicate adequate K at the surface. Potassium concentration was higher in turf than forage varieties. Normalizing K to express uptake as percent of total, shows that 80% of K uptake for these grasses occurs between 378 and 819 HU. Potassium, P and S èrtilization for grass seed production normally occurs in the fall or early spring. The mmobilitv of K in soil minimizes the need for split or late season applications. Typical straw P concentration average less than 0.1% at seed harvest in July Ibr tall fescue and perennial ryegrass (Homeck et al., 1991). The elevated P levels in this study for tall fescue at 1470 HU are attributed to seed being included at final harvest. Perennial ryegrass P levels dropped from the highest of the seven grasses at 819 HU to the lowest at 1470. The reason for this decline is unknown. Other grasses such as Kentucky bluegrass and fine fescue increased in P concentration while the remaining grasses decreased slightly. The high Mn concentration in orchardgrass may be linked to low soil pH, 5.0, at this site. Elevated Mn concentrations in soil solution are one of the principle reasons for yield reduction in low pH soils. Orchardgrass and Kentucky bluegrass have higher soil pH requirements than the other grasses in this study and would thus be more likely to respond to lime and possibly more susceptible to Mn toxicity. 41 Kentucky bluegrass did not show elevated Mn levels. With a lime application, Mn levels in orchardgrass would be expected to decrease. 42 Summary and Conclusions Dry matter accumulation varied between grasses and was linear. Concentration of P. Ca. Mg and S remained constant or fell gradually over the growing season. The tall fescue and perennial ryegrass forage varieties were higher in dry matter than the turf The turf varieties tended to be higher in N, P and K. Tall fescue was higher in K than perennial ryegrass. Turf grasses tended to accumulate some nutrients after 819 Iju. Nutrient accumulation for these grasses was divided into two groups. Perennial ryegrass and orchardgrass which accumulated nutrients in advance of growth and Tall fescue, Kentucky bluegrass and fine fescue which nutrient uptake coincided with dry matter. Fertilization requirements is dependent on grass variety and species. S is generally taken up early, prior to 500 HU and would need to applied between 200 nd 400 HU. Potassium is utilized later, 800 HU, but is applied in the fall making ming less critical. Potassium demand varies by species and variety. Turf and high iry matter grasses such as tall fescue will require more K than Kentucky bluegrass and perennial ryegrass. Nitrogen amounts are difficult to specify from this study. Timing of N to insure fertilizer efficiency can be recommended. Perennial ryegrass and orchard grass need all N applied prior to 800 HU. The other grasses could have an application of 30-40% of spring N applied after 800 HU. 43 20000 2 15000 5000 C ['I 200 600 1000 1400 HEAT UNITS - Fawn,TF v Pennlawn,FF o LinnPR Newport,BG Potomac,OG x Rebel,TF a D Pennfine,PR Figure 3.1. Dry matter accumulation for five grass species by heat unit. 44 100 680 Q) N --------- 1- -I 40 20 1 --P -, 600 1000 1400 Heat Units Figure 3.2. Normalized uptake of N, P. K and S for Fawn, tall fescue by heat unit. 45 100 0 1-.r----,Th.*- 1:: - I N40 2O 200 600 1000 1400 Heat Units Figure 3.3. Normalized uptake of N. P, K and S for Rebel, tall fescue by heat unit. 46 100 1iI ctj a- r;iii a) N 0 IsI 20 z PIII] 600 1000 1400 Heat Units Figure 3.4. Normalized uptake of N, P. K and S for Newport, Kentucky bluegrass by heat unit. 47 100 80 ( N40 ci) EW ç2o 600 1000 1400 Heat Units Figure 3.5. Normalized uptake of N, P. K and S for Permiawn, fine fescue by heat unit. 48 150 o 125 aioo 50 z025 Pills] 600 1000 1400 Heat Units Figure 3.6. Normalized uptake of N, P. K and S for Linn, perennial ryegrass by heat unit. 49 8O ct II °-60 -, N40 2O - -I-.ff--_----- -I 4-- --f'!--'I I!AiIS] 600 1000 1400 Heat units Figure 3.7. Normalized uptake of N, P. K and S for Pennfine, perennial ryegrass by heat unit. 50 100 80 a-o N40 a) 20 600 1000 1400 Heat Units Figure 3.8. Normalized uptake of N. P. K and S for Potomac. orchardgrass by heat unit. heat unit. Table 3.1. Regression model of dry matter accumulation by heat units for five grass species by GRASS REGRESSION COEFFICIENT ----- lb a HU1 ---------- INTERCEPT MODEL USED -2056 Tall fescue (Fawn) 11.89A*** Perennial ryegrass (Linn) 7.40 Kentucky bluegrass 4.32 D*** Perennial ryegrass (Pennfine) 6.28 C*** Fine fescue 6.91 C*** Orchardgrass 6.79 C*** Tall fescue (Rebel) 9.94 B*** DRY WT. a MODEL = 0.97 R2 +b1X1+b2X2...b7X7 *** Significant at P=0.0l U-' H Table 3.2. Dry matter production and nutrient content of five grass species by heat unit. Sampling GRASS Tall fescue, Fawn Perennial ryegrass, Linn ----------------------- 1-IFATUNITS ------------------------250 378 564 819 147() 25(1 378 DRY MATTER 1470 % ---------- 238 1726 4074 7301 15879 3.04 3.14 3.08 1.90 2.00 0 337 646 1469 1564 4006 3390 11394 5427 2.65 3.38 3.37 3.03 2.19 -.-- 1.06 2.57 1278 2521 2093 2731 3760 4331 5290 5366 9841 2.84 2.40 3.29 3.08 3.08 3.01 3.69 2.93 2.48 1.83 2.44 2.05 8659 11726 12521 1717 3.45 4.24 3.11 P11 OS PH 0 RO US % Linn 819 N ITROGEN ---------- lb/a ----------- Kentucky Bluegrass 0 Perennial ryegrass, Penntine () 443 Fine fescue 186 1321 Orchard grass 303 851 Tall fescue, Rebel 710 1370 LSD (0.10) across samplings and grasses Tall fescue, Fawn Perennial ryegrass, 564 (1.29 0.27 0.26 0.20 -.--- 0.26 0.29 0.29 0.31 0.26 0.25 0.33 0.22 Kentucky Bluegrass -.--0.33 Perennial ryegrass, Pennfine -.--0.28 Fine fescue 0.25 0.24 Orchard grass 0.33 0.33 Tall fescue, Rebel 0.20 0.26 LSD (0.10) across samplings and grasses 1.68 3.05 1.66 1.98 0.47 POTASSIUM ------------ 0.24 1.74 % 2.72 2.95 3.04 2.88 1.87 0.11 1.84 0.27 0.34 2.03 2.48 2.25 2.83 2.66 2.25 0.34 0.16 0.35 0.17 0.18 0.05 2.02 1.72 2.78 2.39 2.79 2.33 3.26 2.76 3.26 2.55 3.27 2.83 0.23 0.29 0.25 -.-1.82 2.45 1.94 1.55 1.84 2.03 2.02 2.18 0.29 Ui t\) Table 3.2. Continued. ----------------------- HEATUNITS Sampling Tall fescue, Fawn Perennial ryegrass, Linn 250 378 564 819 1470 250 378 0.36 0.40 0.25 0.36 0.18 0.18 0.16 0.14 0.14 -.---.--- 0.42 0.39 0.52 0.48 0.45 0.34 0.47 0.44 -. -. 0.15 0.14 0.16 0.14 0.15 0.13 0.11 0.44 0.35 0.42 0.42 0.44 0.37 0.28 0.34 0.44 -. 0.13 0.22 0.26 0.14 0.13 0.20 0.23 0.16 0.13 0.19 0.22 0.15 0.13 0.14 0.19 0.11 0.31 0.42 0.45 0.12 0.40 0.36 0.30 0.19 0.14 -.--- 0.25 0.26 0.28 0.24 0.21 -.--0.27 0.26 0.21 0.32 0.29 0.34 0.33 and grasses 0.28 0.24 0.29 0.30 0.24 0.19 0.23 0.24 -.--- 0.19 0.10 0.10 0.15 0.19 0.014 ZINC % ------------ Kentucky Bluegrass Perennial ryegrass, Pennfine Fine fescue Orchard grass Tall fescue, Rebel LSD (0.10) across samplings 1470 0.27 SULFUR Linn 819 CALCIUM MAGNESIUM ----------% ------------------------ % ---------- Kentucky Bluegrass Perennial ryegrass, Pennfine -.--0.62 Fine fescue 0.23 0.31 0.33 0.34 Orchard grass Tall fescue, Rebel 0.31 0.40 LSD (0.10) across samplings and grasses Tall fescue, Fawn Perennial ryegrass, 564 ppm ---------- 21.3 20.5 28.8 24.0 11.8 0.15 0.10 26.8 22.5 30.5 25.0 19.3 17.3 14.3 13.0 0.16 0.12 0.16 0.16 0.038 30.0 31.5 23.0 21.5 23.0 31.0 20.3 22.8 23.5 17.3 15.0 14.0 15.3 14.3 12.3 6.7 30.0 28.0 20.3 15.8 Ui w Table 3.2. Continued. Sampling 250 378 564 819 1470 250 378 MANGANESE Tall fescue, Fawn Perennial ryegrass, Linn 190 160 119 235 209 152 164 152 163 142 189 125 192 221 112 Kentucky Bluegrass Perennial ryegrass, Pennfine 246 Fine fescue 290 345 320 Orchard grass 303 Tall fescue, Rebel 199 189 LSD (0.10) across samplings and grasses Tall fescue, Fawn Perennial ryegrass, Linn 208 320 236 270 159 162 142 6.5 BORON ppm 1.0 3.0 -.-.- 4.0 0.8 Kentucky Bluegrass Perennial ryegrass, Pennfine -.4.5 Fine fescue 9.0 4.5 11.3 5.3 Orchard grass Tall fescue, Rebel 6.8 1.5 LSD (0.10) across samplings and grasses 1470 ppm ---------- 5.3 40.4 819 COPPER ------------ ppm ----------204 564 7.0 10.8 5.0 6.0 5.3 3.3 3.2 4.8 6.3 4.4 5.4 2.8 3.3 3.0 4.0 5.0 6.3 8.3 5.3 4.5 6.2 6.7 4.9 3.5 3.0 3.5 3.5 3.4 3.5 3.4 4.0 0.63 -----------0.3 6.3 4.3 0.3 2.3 0.8 8.3 8.3 2.3 2.0 2.8 5.3 0.8 0.5 1.5 1.3 7.5 8.3 9.3 6.5 1.8 Ui Table 3.3. Uptake of N, P, K, S. Ca and Mg at physiological maturity for five grass species. Nutrient N P K S Ca Mg UPTAKE AT 1470 HU GRASS lb/a --------------56 52 23 43 27 48 56 22.5 12.6 5.6 10.8 8.2 232 262 22.3 16.7 5.6 15.9 9.9 17.9 19.9 34 3.1 6.7 2.3 Tall fescue, Fawn Perennial ryegrass,Linn Kentucky Bluegrass Perennial ryegrass,Pennfine Fine fescue Orchard grass Tall fescue, Rebel 304 30.6 295 123 141 162 12.2 18.9 15.7 265 203 247 29.9 20.8 22.6 175 121 184 176 LSD (0.10) 47 4.8 17.1 23.7 u-I u-i 56 References Doerge, T.A., H. Gardner and T.L. Jackson. 1982. FG 6: Fine fescue seed. Oregon State Univ. Extn. Ser. Corvallis, Oregon. Doerge. T.A., H. Gardner and T.L. Jackson. 1982b. FG 44: Blue grass seed. Oregon State Univ. Extn. Ser. Corvallis, Oregon. 1)oerge. l.A., H. Gardner, T.L. Jackson and H. Youngberg. 1982c. FG 45: Orchardgrass seed. Oregon State Univ. Extn. Ser. Corvallis, Oregon. I)oerge. T.A., H. Gardner and H. Youngberg. 1983. FG 36: Tall fescue seed. Oregon State Univ. Extn. Ser. Corvallis, Oregon. l)oerge, l.A., H. Gardner T.L. Jackson, and H. Youngberg. 1982d. FG 46: Perennial ryegrass. Oregon State Univ. Extn. Ser. Corvallis, Oregon. 1ngelstad. O.P., 1968. Use of multiple regression in fertilizer evaluation. Agron. J. 60:3 pp.327-329. ierwing. J.. R. Gelderman and E. Twidwell. 1991. Nitrogen and phosphorous fertilization of cool-season grass. S. Dakota St. Univ. Brookings S. Dakota. irunes, D.W., J.W. Huang. F.W. Smith, P.K. Joo and D.A. Hewes. 1992. Potassium effects on minerals and organic acids in three cool-season grasses. J. Plant Nutr. 15:1007-10025. Ilillard, J.B., V.A. Haby and F.M. Hons. 1992. Annual ryegrass response to limestone and phosphorous on an ultisol. J. plant Nutr. 15:1253-1268. Horneck, D.A., and J.M. Hart. 1988. A survey of nutrient uptake and soil test values in perennial ryegrass and turf type tall fescue fields in the Willamette Valley. Iii: H.W. Youngberg (ed.). 1988 Seed Production Research. Dept. of Crop Science, EXT/CrS 74. p. 13-14. Oregon State University, Corvallis, OR. Horneck, D.A., J.M. Hart. 1 992b. Third-year response of tall fescue and perennial ryegrass to lime and phosphorus during a four year study. pp. 6-8. In: W.C. Young III (ed.) 1991, Seed Production Research. Dept. of Crop Science. EXT/CrS 89. Oregon State University, Corvallis, OR. 57 Horneck, D.A., J.M. Hart. W.C. Young III and T.B. Silberstein. 1992a. Potassium for grass seed nutrition. pp. 3-6. In: W.C. Young III (ed.) 1991. Seed Production Research. Dept. of Crop Science, EXT/CrS 80. Oregon State University, Corvallis, OR. Kuhlmann. K., 1990. Importance of the subsoil for the K nutrition of crops. Plant and Soil. 127:129-136. ()rlovius, K., 1992. Potash fertilization of grassland with special consideration of K removals. Potash review. 1:1-7 Youngberg, H.W., W.C. Young. 1990. Oregon forage and turf grass variety seed trial, 1986-87. Dept. of Crop Science, Oregon State University, Corvallis, OR.Ext/CrS 80. Corvallis Or. CHAPTER 4 EFFECT OF SOIL K ON PERENNIAL RYEGRASS AND TALL FESCUE Introduction Grass seed is grown on 370.000 acres in the Willamette Valley. Oregon. Tall fescue and perennial ryegrass accounted for 50% of the acreage and 100,000.000 dollars in gross sales during 1993. Tall fescue and perennial ryegrass produce 5,000 to 20,000 kg straw ha1 per year. Historically, residues left after seed harvest were burned which recycled elements such as K. Over the last five years changes to systems that harvest straw have interrupted nutrient cycling that occurred with Nurning. Potassium once left on the field is now being removed in straw. During 1985 ver 200.000 acres were burned in Oregon, by 1993 only 70,000 acres were burned. he decrease in burned acreage is more dramatic when considering grass seed acreage rom 1987 to 1993 increased 21%. Soil K can be significantly influenced after a ingle years residue management (Horneck et al., 1991). Soil K and K removal rates have been studied in grasslands (Orlovius, 1992), but only recently in grass seed lelds. A survey of 77 Willamette Valley perennial ryegrass and tall fescue field's in 1 987 showed K soil tests averaged 300% of Oregon State University recommendations (Horneck & Hart, 1988). Current OSU fertilizer guides state 100 mg kg1 as K soil test critical level for perennial ryegrass and tall fescue (Doerge et al.. 1982 Doerge et al., 1983). Grass seed crops utilize between 50 and 400 kg K ha' depending on grass type, variety and soil test K. Uptake when soil tests were 144 mg K kg' varied between 196 kg K ha1 for perennial ryegrass and 300 kg K ha' for tall fescue (Horneck et al., 1993). Spring wheat K uptake has been shown to occur from different layers in the soil depending on K soil test (Kuhlmann, 1990). Potassium utilized by wheat from the subsoil was dependent on topsoil K. Where surface K levels were high less K was obtained from subsoil. However, when surface soil K levels were low wheat was able 59 to obtain a higher percentage of its K from the subsoil. To get an adequate prediction of K response subsoil and surface K levels need to be measured. Perennial ryegrass and tall fescue differ in dry matter production, K uptake and K concentration at seed harvest (Horneck et al., 1993). TaIl fescue produces more dry matter and has higher K concentrations than perennial ryegrass. Potassium concentration in tall fescue and perennial ryegrass increases to 32.5 g kg1 during the late winter and spring months then decreases to less than 20 g K kg prior to seed harvest. This increase then decrease depending on grass maturity was observed where soil test K was 144 mg kg1. Conversely, Ca and Mg gradually decreased over the growing season for tall fescue and perennial ryegrass. Potassium concentrations required for cool-season grass seed growth are unknown. Survey results indicate that the 100 mg kg1 K critical soil test level are thought by growers to be conservatively below grass need even though a K deficiency n cool-season grass production has never been documented in the Willamette Valley, )regon. Logistic growth models are useful to describe how these grasses respond to soil K. Logistic growth models have been used to describe tall fescue and perennial ivegrass response to N applications (Overman and Wilkenson. 1992 Overman and Wilkenson. 1993). Models have also been used to describe nitrogen application spacing in fescue pastures (Vigil et al.,1993). Logistic growth models in this study were used to approximate affect soil test K has on K concentration in tall fescue and perennial ryegrass from field trials. The objectives of this study are to determine the responsiveness of tall fescue and perennial ryegrass to soil test K. From these results fertilizer recommendations will be estimated. Materials and methods Four sites planted to perennial ryegrass and tall fescue with high and low soil test K were located in the Willamette Valley, OR. Each site was treated with open field burn and vacuum sweep residue management treatments, in combination with potassium fertilization, K-0 and K-+. Initial 0-15 cm K soil test levels for the two tall fescue sites were 218 and 55 mg kg* The two perennial ryegrass sites had 164 and 78 mg K kg1. Fall K-+ rates were 33.5 kg ha for 1988 and 1989 and 100 kg ha' in 1990. The high K tall fescue site was located on Woodburn SiL a fine-silty, mixed. mesic Aquultic Argixeroll. The remaining three sites were Dayton SiL fine, montmorillonitic, mesic, Typic Albaqualfs. The tall fescue cultivars were Martin and Rebel II and the perennial ryegrass were Regal and Pleasure. Straw yield, seed yield and soil test levels were sampled annually at each site. Sites were soil sampled in June at the 0-2.5 and 0-15 cm depths, 1990 soil sampling also included samples to a depth of 60 cm. Tissue and seed samples were analyzed for K, Ca and Mg. Soil samples were analyzed for 2:1 pH, Bray-Pi, K, Ca and Mg as described by Horneck et al., 1989. At the 218 mg K kg site the annual burn plots were moved after the 1 989 harvest as the result of the stand being destroyed from the burn. Data was analyzed in ANOVA in a factorial arrangement with years as the first split. The second split was fertility which were sites chosen for K level. Grass type, straw management and K application were the remaining factors taken in sequence. Experimental comparisons between fertility for factors such as seed and straw yield have been confounded with site and variety differences. Logistic response models (EQ 1) used to estimate foliar K concentration as a function of soil test level (5) have three parameters: K(max) the maximum, R a slope factor and N(0) the minimum. Model parameters were calculated using PCNONLIN version 4.2. The rate function, equation 2, of delta straw to delta soil is the first derivative of equation 1. 61 Straw K concentration = K(max I +(K(max)-N(0)) N(0) (1) * e Straw K concentration / Soil = R*S((K(max)S)/K(max)) (2) 62 Results and discussion Three year average seed yields were not affected by K application (Table 4.1). In addition, seed yield averaged across entire experiment produced no difference between K-+ at 1499 kg seed ha1 and K-U at 1487 kg seed ha1. Similarly, straw management did not affect seed yield. Seed yield response to K fertilization, though not significant, was evident where residues were removed (vacuum sweep: K-+=1289 kg ha', K-0=1235 kg ha'). However, three year averages show no significant advantage to K fertilization where residues were removed at either the low or high K soil test sites for either tall fescue or perennial ryegrass (Table 4.1). Perennial ryegrass yielded more seed than tall fescue, 1601 kg ha1 vs 1384 kg hafl, respectively. Seed yield was affected by year and fertility (location). The high K sites tended to have higher seed yields but this outcome was dependent on year and grass. Seed yield differences between years appear to be linked to climatical factors not stand age. Tall fescue seed yields were 1126 kg ha1 in 1989, 1741 kg ha' in 1990 and 1285 kg ha1 in 1991. Potassium concentration in the seed was affected by fertility, grass type and straw management. Differences in K due to treatments were small (Fig 4.1). Mean K concentration was 5.3 g K kg' seed for this study with a 5.5% coeficient of variation (CV). Because of low the low CV, least significant differences were 0.12 g K kg' seed. Perennial ryegrass seed had higher K concentrations than tall fescue, 5.4 g K kg seed vs 5.2 g K kg1 seed, respectively. Potassium concentration in seed for the straw management treatments averaged 5.4 g K kg1 for the burned treatments and 5.2 g K kg (p=O.O5) where residues were removed. Seed from the high K sites had K concentrations averaging 5.6 g K kg' while average seed K concentration at the low K sites was 5.0 g K kg1 seed. Potassium concentrations across fertility is confounded by location and grass variety. Seed K concentration was not effected by K application when averaged across fertility. However, the fertility by K application and straw by K application interactions were significant (p=O.OS). Potassium applications increased (33 seed K concentration only at the low fertility sites from 4.8 g kg1 to 5.1 g kg1. Seed K concentration increased from 5.0 g kg' to 5.4 g kg due to K applications where residues were harvested, K fertilization had no effect where residues were burned. Potassium fertilization had no effect on seed K at the high K sites or where residues were burned. Potassium uptake in the seed averaged 5.12 kg ha1 with a maximum of 10 kg 1ia. Grass type, fertility and K application did not affect K uptake in seed. Soil test K did not effect seed K level or K uptake (Fig. 4.1). Where residues were removed K uptake by seed was 6.2 kg K ha compared to burned treatments with 5.7 kg K ha* Several four way and the five way interactions were significant for seed K uptake (pO.l 0). Significant interactions between years and fertility are not surprising due to climatical differences between years and its effect on seed yield. Differences between sites and varieties contribute to interactions with fertility. Differences in seed K concentration due to treatments, though statistically significant, are of minimal importance when considering total K removal where residues are harvested. Removal where straw is burned, while still less than 10 kg K ha1, is the primary K loss from the field. Straw yield increased from 4463 kg ha' in 1989 to 8337 kg ha' in 1991 averaged across grasses and treatments. Perennial ryegrass straw yield significantly increased from 3830 kg ha' in 1989 to 7060 kg yield increased from 5095 kg ha1 ha1 in 1991 while tall fescue straw to 9613 kg ha1. Increases in straw yield appears linked to stand age and less dependent on climatical factors than seed yield. Tall fescue produced more straw, 7936 kg had, than perennial ryegrass. 5712 kg ha'. Straw management and K application did not effect dry matter. The K application by fertility interaction was significant at p=0.07. Potassium fertilization at the high K sites depressed straw yield (K-+6694 kg ha', K0=r6808) where at the low K sites fertilization tended to increase straw yield (K-+=6951 kg ha', K-06839 kg ha'). Three year straw yield averages for the vacuum sweep treatments were higher at all four sites where K was applied (Table 4.1). Conversely annual burn straw yields were 64 lower at all four sites where K was applied. These differences result in a significant (p=O.O7) K application by fertility interaction. Potassium concentration in tall fescue and perennial ryegrass straw was affected by soil test K, grass type, straw management and K application (Fig. 4.1). A significant Fertility by K application interaction showed that K concentration in tall fescue and perennial ryegrass straw increased with K application at the low K sites. Potassium applications at the high K sites did not effect straw K concentration. Tall fescue at the low K sites had K concentrations averaging 5.9 g K straw was harvested and 8.6 g K kg1 kg1 straw where where straw was burned. Potassium concentration in perennial ryegrass straw at the low K sites behaved similarly to tall fescue. Where perennial ryegrass residues were removed K concentrations averaged 6.6 g K kg1 kg1 straw and where residues were burned 9.5 g K straw. Lower K levels due to vacuum sweeping appear to be the result of lower soil K. Soil test K at the low K perennial ryegrass site was 49 mg K kg1 soil where residues were harvested and 69 mg K kg where residues were burned. Straw K concentration independent of year is a function of soil test K (Fig. 4.1) Soil K at the 2.5 cm and 15 cm depths were affected (p=zO.05) by residue management. The three year 2.5 cm soil K average for all the burned plots was 304 mg K kg1 soil, the vacuum sweep treatments averaged 188 mg K kg1 soil. Tall fescue because of larger amounts of dry matter and higher K concentrations in straw affected soil K levels more than perennial ryegrass. Tall fescue 15 cm soil tests were 394 mg K kg' soil where residues had been burned. Where residues were harvested soil K was 201 mg K kg' soil, a difference of 193 mg K kg1 soil. Three year averages for the perennial ryegrass treatments differed by 39 mg K kg' soil at 15 cm. Potassium concentration in tall fescue and perennial ryegrass straw increased with increasing soil test K at the low K sites. Potassium concentration in tall fescue and perennial ryegrass was unaffected by soil test K at the high K sites. Potassium concentration in tall fescue and perennial ryegrass were similar to each other when 65 soil tests were below 100 mg K kg' soil. Where fertility was high, perennial ryegrass averaged 13.4 g K kg' where as tall fescue averaged 21.9 g K kg (Table 4.2. Fig. 4.1). Potassium concentrations are soil test dependent. Differences between grasses in this study are confounded by location. However, based on earlier uptake work, turf type tall fescue will be higher in K concentration than turf type perennial ryegrass (Homeck et al., 1993). Foliar K of 13.4 g kg' and 21.9 g kg' for perennial ryegrass and tall fescue represents K at harvest, respectively, K concentrations during winter and spring will be higher. Logistic growth models (EQ. 1) can be used to predict straw K concentration relative to soil test K (Fig 4.2, Table 4.2). Logistic growth models help us understand how perennial ryegrass and tall fescue respond to K. The model parameter N(0) is predicted even though no concentrations below 4 g K kg exist in this study. For adequate growth perennial ryegrass and tall fescue probably need 5 g K kg in straw at harvest. Potassium straw concentration in tall fescue and perennial ryegrass respond similarly to soil K when soil tests are below 100 mg kg' (Fig 4.2). Potassium concentrations in perennial ryegrass and tall fescue straw behave differently when soil test K exceeds 100 mg K kg soil (Table 4.2). Tall fescue and perennial ryegrass reach maximum K concentration at different soil test K. The similarities and differences between tall fescue and perennial ryegrass are evident in the predicted model parameters. Similar slopes (R) and N(0) in the logistic growth model between grass species reinforce the similarity of K concentrations to soil test K (Table 4.2). A predicted maximum K concentration for tall fescue of 21.9 g kg' and for perennial ryegrass had 13.4 g kg* Maximum K concentration was the only model parameter that was different between the two grass species. Change in straw K concentration relative to soil test K (E. 2) is plotted in Figure 4.3. Perennial ryegrass and tall fescue reach maximum rate of K concentration change at 45 mg K kg1 soil and 90 mg K kg1 soil test K, respectively. These peaks are where soil K has its largest effect on tissue K concentration and is the inflection point from the logistic growth model. Growth due to additional K has ceased, 66 therefore additional K to the plant either via fertilizers or increased soil test K is not being diluted but used to increase K concentration in the straw. Rate of change in straw K concentration due to increased soil K, decreases because foliar K concentration as it approaches its maximum changes with decreasing efficiency. The law of diminishing returns continues until soil K no longer has an effect on straw K. Maximum K concentration is reached. Maximum K concentration in straw at harvest br perennial ryegrass occurred at 200 mg K kg1 soil and 325 mg kg1 for tall fescue. Potassium uptake by tall fescue and perennial ryegrass in straw is effected by K level and dry matter production. Tall fescue not only has higher K concentration it also produces more dry matter than perennial ryegrass. These differences between perennial ryegrass and tall fescue species are reflected in K uptake and the fertility by grass interaction (Fig. 4.4). tall fescue and perennial iyegrass were similar in K uptake at low fertility sites but significantly different at the high sites. Tall fescue can remove in excess of 300 kg K ha1 where perennial ryegrass has the potential to remove 100 kg K ha1. Potassium uptake in figure 4.4 is what is removed when straw is swathed at a 10 -15 cm height, crew cutting then vacuum sweeping removes additional K. Potassium uptake response to soil K is dependent on year because dry matter increased with year. Foliar K seems to be more independent of year. Open field burning or chopping back straw is a 25-300 kg ha1 K application depending on soil test and the amount of dry matter. Straw's effect on soil K was emphasized by straw management (vacuum sweep=135 mg kg1, bum=l 74 mg kg') increasing soil K at p=O.O5 where as K fertilization did not (K-+=160 mg kg1, K-0=150 mg kg1). Open field burning or chopping back straw is in effect a 25-300 kg ha1 K application depending on grass species, soil test and dry matter produced. Straw's effect on soil K was emphasized by open field burning increasing soil K where K fertilization did not. Soil test K levels above 50 mg 100 mg kg1 kg1 for perennial ryegrass and are associated with Luxury consumption where the plant is taking up more K than is necessary for it to manufacture seed or straw. Why grass varieties, species and cultivars use different amounts of K in unclear. The preference for K over Ca Na. and Mg may be linked to soil mobility, preference for charge balance and a lack of plant root regulation (Table 4.3). Whether higher foliar K concentration improves winter hardiness or disease resistance has yet to be determined. Straw K concentrations as low as 3.5 g K kg straw were observed in this study. A K concentration of 6 g K kg has been shown to be necessary to maintain K specific metabolic processes in perennial ryegrass in a greenhouse study (Baily, 1989). Low K concentrations decreased straw yield when soil test K was below 100 ppm and only showed indications of effecting seed yield where straw was removed. Seed yield and straw yield tended to increase with K application, at the low the low K sites, where straw was harvested due to lower tissue and soil K concentrations.. Potassium concentration in residue effected Ca and Mg concentrations (Table 4.3). Potassium's affects on Ca and Mg has been measured by Grunes et al., 1992) Magnesium and Ca concentration were negatively correlated with K in straw at harvest. Magnesium had correlation coefficients with K of -0.86 for perennial ryegrass and -0.90 for tall fescue. Calcium was not as correlated with K as Mg with correlation coefficients averaging -0.61. Calcium and Mg were positively correlated with each other. Summary and Conclusions Managing straw K concentration is of minimal importance where open field burning and chopping back straw are used to manage residues because K is recycled. Ierennial ryegrass can take up 112 kg K ha1 when soil tests are greater than 150 mg K kg1 soil. Potassium concentration will not increase when soil tests exceed 150 mg K kg1 soil. When soil test are less than 100 mg ha1 kg1 K, a K application of 30-50 kg K should supply enough K for vegetative and reproductive growth in perennial ryegrass. When K soil tests are greater than 100 mg K kg1 soil, a K application is unwarranted. Tall Fescue can utilize 300 kg K ha when soil tests are in excess of 300 mg kg1 K. Tall fescue utilizes more K than perennial ryegrass. Higher K concentrations dnd more dry matter yields a larger potential for K uptake with tall fescue. Tall fescue nd perennial ryegrass K concentration have the same slope in their response to soil cst K. Potassium concentration will not increase when soil tests exceed 325 mg K kg soil. Potassium concentrations above 10.0 g kg1 are not needed for seed or vegetative yield. Fertilizer applications of 50-100 kg K ha' should only be considered for tall fescue when soil tests are below 150 mg kg* When soil K is above 150 mg kg', K fertilization will only increase K content and uptake in the straw. 30 a 0) a, 0 20 U) U) Cl) o10 S 1. 4-. C') 0 0 100 200 300 400 0-15 cm Soil K, mg/kg TALL FESCUE TF SEED 500 EJ PERENNIAL RYE OPR SEED Figure 4.1. Effect of soil K on straw and seed K for tall fescue and perennial ryegrass. 70 25 a) 20 r2=.85 --------- TALL FESCUE - 15 --- r2=.69 ------------- 10 H PERENIAL RYEGRASS I' Cl) 5 iI 0 100 200 300 400 0-15 cm Soil K, mg/kg Figure 4.2. Predicted straw K for tall fescue and perennial ryegrass from soil K. 71 D) - D) 0(I) I 0 100 200 300 400 0-15cm Soil K, mg/kg Figure 4.3. Rate of change for straw K for tall fescue and perennial ryegrass due to soil K 72 350 -c - 300 U 250 H 0 D150 100 a H 50 0 100 200 300 400 500 0-15 cm SOIL K, mg/kg - TALL FESCUE PERENNIAL RYE Figure 4.4. Effect of soil K on straw K uptake for pereimial ryegrass and tall fescue. 73 Table 4.1 Seed yield arid straw yield three year averages for tall fescue and perennial rvegrass by K application, residue management and K fertility. Treatments ---------- 1ERT GRASS RESIDUE KAPP n SEED STRAWY -kgha1 low TF FC K-+ 12 1289 7822 low TF FC K-U 12 1235 7698 low TF AB K-+ 12 1268 7778 lOW TF AB K-U 12 1260 8370 low PR FC K-+ 12 1528 5558 low PR FC K-U 12 1503 5460 low PR AB K-+ 12 1531 6198 low PR AB K-U 12 1536 6277 high TF FC K-+ 12 1655 8796 high TF FC K-U 12 1572 8465 high IF AB K-+ 12 1339 7117 high TF AB K-U 12 1449 7435 high PR FC K-+ 12 1659 5887 high PR FC K-U 12 1659 5364 high PR AB K-+ 12 1716 5433 high PR AB K-U 12 1675 5514 NS NS LSD 0.05 within grass and fertility FC=flailchop and vacuum sweep, AB=annual burn FERT=K fertility 74 Table 4.2. Logistic Growth model parameters for foliar K response of TF and PR to soil K. Model parameter Estimate TF Standard Error PR TF PR ---gKkgstraw--K(Max) 21.9** 13.4** 0.559 0.342 2.9 2.7 0.706 0.746 (0) g kg1 straw per mg kg' soil R (Slope) 0.217 0.322 0.046 0.0655 ** Significant at p=O.OS [able 4.3. Correlation of foliar K in TF and PR with Ca and Mg. GRASS TYPE PR TF nutrient Mg K ---------------------------------- r -------------0.86*** 0.68*** 0.545*** 0.80*** 0.58*** Mg I Significant at p=O.O5 Ca Mg Ca 75 References Bailey, J. S., 1989. Potassium-sparing effect of calcium in perennial ryegrass. J. Plant Nutrition. 12:1019-1027. Doerge, T.A.. H. Gardner, T.L. Jackson, and H Youngberg. 1982. Perennial ryegrass. FG 46. Or. St. Univ., Corvallis, OR. Doerge. TA., H. Gardner, and H Youngberg. 1983. Tall fescue. FG 36. Or. St. Univ.. Corvallis, OR. Grunes, D.L., J.W. Huang, F.W. Smith, P.K. Joo, and D.A. Hewes. 1992. Potassium effects on minerals and organic acids in three cool season grasses. J Plant Nutr. 15: 1007-10025. 1-lorneck. D.A., and J.M. Hart. 1988. A survey of nutrient uptake and soil test values in perennial ryegrass and turf type tall fescue fields in the Willamette Valley. jfl: H.W. Youngberg (ed.). 1988 Seed Production Research. Dept. of Crop Science, EXT/CrS 74. p. 13-14. Oregon State University, Corvallis, OR. Torneck. D.A., J.M. Hart, and W.C. Young III. 1993. Uptake of N.P.K and S by five cool-season grass species. 111J992 Seed production research at Oregon State University. W.C. Young III ed. Dept. Crop and Soil Sci, Or St Univ. Ext/CrS 93. pp. 20-23. ilomeck. D.A., J.M. Hart, W.C. Young III and T.B. Silberstein. 1991. Potassium for grass seed production. th 1990 Seed production research at Oregon State University. W.C. Young III ed. Dept. Crop and Soil Sci, Or St Univ. Ext/CrS 4/91. pp. 20-23. Kuhimanri, H., 1990. Importance of the subsoil for the K nutrition of crops. Plant and Soil. 127:129-136. Orlovius, K. 1992. Potash fertilization of grassland with special consideration of K removals. Potash Review. 1:1-8 Overman, A.R., and S.R. Wilkenson. 1992. Model evaluation for perennial grasses in the Southern United States. 1992. Agron J. 84:523-529. Overman, A.R., and S.R. 1993. Wilkenson.Modeling tall fescue cultivar response to applied nitrogen. Agron J. 85:1156-1158. 76 Vigil, M.F., D.E. Kissel, M.L. Cabrera, C.W. Raczkowski. 1993. Optimal spacing of surface-banded nitrogen on Fescue. Soil Sci Soc Am J 57:1629-1633. 77 CHAPTER 5 SOLUTION P [N TWO SOILS AMENDED WITH LIME AND P Introduction Results from a 1987 survey of Willamette Valley grass seed fields showed stratification of P and soil pH within the surface 15 cm of soil. Soil pH averaged 0.2 pH units lower for 2.5 cm samples than observed in 15 cm samples. The survey also showed a majority of the 77 fields sampled had pH values less than 5.2 and P levels greater than 50 mg kg1 (Horneck and Hart, 1988). The average Bray-Pi soil test in the survey was 80 mg kg1. Recent concerns regarding P in surface waters broadens P management to an environmental concern. Oregon Extension recommendations are that soil pH should be above 5.5 and no fertilizer P is recommended when soil P is greater than 25 mg kg1. Growers continue to apply an average of 45 kg soil tests in excess of 50 mg P kg1 ha1 P despite (Hart et al., 1988). The continued P application by rass seed growers when Bray-P 1 soil tests average more than twice critical levels is \'hat prompted this study. Soil pH is cited as a regulator of P availability (Brady,1984). Where soil pH is high, Ca precipitates P from the soil solution. When pH is low, Fe and Al are the main P complexers. Thus, a soil pH of 6.5-7.0 should be ideal for P availability. The relationship between P availability and the presence of complexing elements is not well defined and probably soil specific. Jia and Dahnke, 1994, showed that soil pH and liming had little effect on P and conventional P soil tests. Phosphorus (P) is logically a limiting element in cool-season grass nutrition due to its immobility in cool wet soils. Cool-season grasses in Oregon are grown on soils that are saturated much of winter and spring with soil temperatures below 5 to 100 C. During summer and fall cool-season grasses are dormant when soils in Western Oregon are dry and warm. However, no response to P has been observed on low (<12 mg kg1) soil test fields (Horneck et al., 1992) where pereimial ryegrass has been grown for seed. 78 Perennial ryegrass seed yields at Saddle Butte, Oregon did not respond to P applications or placement when the Bray-Pl was 12 mg P kg' soil. Seed yield where no P was applied in 1989 averaged 1091 kg ha* Where P was applied at 34. 68 and 102 kg P ha1 seed yields averaged 1012, 1015 and 947 kg had, respectively. Interactions between placement and P-rate were not significant. Seed yields in 1990 at Saddle Butte averaged 1417 kg ha1 but also showed no response to P-rate or P placement. Tall fescue seed yields at Hyslop, Oregon have not responded, as expected. to P applications where Bray-P 1 was 80 mg P kg soil. The lack of yield response to P fertilization by these grasses and the inability of the Bray-P 1 to predict P responses relative to current critical levels led to investigation of the soil solution. The soil solution is the plants primary source of P. Interactions of inorganic, mineral and organic P forms with elements in the soil solution are not well understood. Conventional P soil tests such as the Bray-P 1 and 1sen (NaHCO3) need to account for variation in plant P accessibility to adequately predict plant responses. Since neither lime or P applications increased seed yield the objectives for this project were to: 1) Assess soil lime and pH affects on soil solution nutrient concentrations. 2) Evaluate the effect of P rate with varying soil pH on soil solution nutrient concentrations. 3) Investigate why perennial ryegrass was not responding to P applications at Saddle Butte, Oregon, the low P site. 79 Materials and methods Two experimental sites with low soil pH (5.2) were established with perennial turf-type grasses for seed production. Perennial ryegrass was seeded (Lolium perenne cv SR 4000) in Bashaw clay (typic pelloxerert) at Saddle Butte, Oregon where 15 cm Bray-P was 12 mg kg* Tall fescue (Festuca arundinacea cv Falcon) was seeded in \Voodburn silt loam (aquultic Argixeroll) at the Oregon State University Hyslop Crop and Soil Science Farm where 15 cm soil test P was 60 mg kg* A split plot design was used with three and four replications in a factorial arrangement for Saddle Butte and Hyslop, respectively. The main plots were lime with 0, 4.5 and 9 Mg ha1. The 9 Mg ha lime rate was incorporated prior to planting in 1989. The 4.5 Mg ha lime rate was surface applied in the fall of 1990. Subplots were annual P rates of 0, 34, 68 and 102 kg P ha and P application method, banded (spring and fall) and broadcast. Ilyslop and Saddle Butte were both annually treated with oxyfluoren and diuron in the fall in combination with spot spraying by hand with glyphosate. Nitrogen was applied at 45 kg N ha1 in the fall and 134 kg ha1 in the spring as ammonium sulfate. Samples for soil solution extraction were collected in the spring of 1991. Thirty to 40 subsamples per plot were taken at the 0-2.5 cm depth. The three lime and the 0 and 34 kg P ha1 broadcast treatments were sampled at both sites. Samples were immediately refrigerated and extracted within 24 hours. Custom 100 ml centrifuge cups similar to ones described by Adams et al. (1980) were filled with wet soil and spun at 1000 g for one hour. Two solution extracts were combined into a single sample. Weights of samples and centrifuge cups were taken prior to extraction. Samples from the centrifuge cups were oven dried at 105 C for 48 hours and weighed after extraction to determine percentage of water extracted. After solution extraction the unused soil was air dried and analyzed conventionally for Bray-P 1, NaHCO3-P, 1:2 pH, K. Ca, and Mg (Horneck et al., 1989). Solution extracts were analyzed immediately for pH. After pH measurement solutions were frozen, then as time allowed thawed and analyzed for orthophosphate (P) and nitrate as described by Horneck et al. (1989). The solutions were also analyzed for total phosphorus (TP), Ca, Mg, K, Na, S, B, Zn, Cu, Mn, Fe, and Al by inductively coupled plasma (ICE). Organic P (P0) was calculated by subtracting P from TP. SAS was used to compute correlation coefficients, ANOVA and mean separations. Because of differences in mineralogy, crop grown, drainage and Bray-Pi levels the two sites were analyzed independent of each other. E31 Results and Discussion The Bashaw soil at Saddle Butte is reported to have 55% to 70% clay dominated by montmorillonite, while the Woodburn at Hyslop has 10 to 20% clay (Patching. 1987). Water contents prior to extraction for the Bashaw and Woodburn soils represent close to field capacity. Saddle Butte soil samples averaged 42% water on an oven dry weight basis before extraction and 36% after. The soils from Hyslop averaged 25% water prior to extraction and 19% after. Differences in water content reflect known differences in mineralogy and clay content of the two soils. The effects of lime and P will primarily be viewed independently since few significant interactions existed between lime application and P rate (Table 5.1). Lime application increased soil and solution pH (Table 5.2). The 9 Mg ha' lime rate increased soil pH from 4.8 to 6.4 at both Saddle Butte and Hyslop. The 4.5 Mg ha surface application increased soil pH to 5.3 at Saddle Butte and 5.4 at Hyslop. A larger separation in solution pH due to lime treatments was observed compared to soil pH. Solution and soil pH were similar where lime was applied (Table 5.2). Solution pH for the unlimed treatments were lower than soil pH by 0.74 at Saddle Butte and 0.54 at Hyslop. The similarity between soil pH and solution pH where lime was added is thought to be the result of continued buffering by undissolved lime. Where lime had not been added CaCO3 was not present lowering the buffering capability of soil solution pH. Lime has been shown to persist for over three years depending on incorporation and initial soil pH (Doerge and Gardner, 1985) Increased pH due to lime applications decreased the solubility of Mn, Al. Fe and Zn compounds (Fig 5.1.). Solution metal concentrations were significantly changed by liming and a function of either soil or solution pH (Table 5.3, Fig. 5.1). Metals that did not have significant correlation coefficients such as Fe, and Cu still appear to be related to pH. The relationship shown in Figure 1, where, maximum concentration increases as pH decreases, indicates metal solubility is controlled by soil pH. A similar relationship exists for the Saddle Butte site except for aluminum 82 concentration which was constant across soil pH. Poor correlations exist between soil pH and Mg, Fe, Al and CU, either from lack of concentration dependency or higher and wider concentration ranges at low pH with lower and narrower ranges at higher pH. Correlation coefficients measure linear variability and are a poor indicator of this type of variability. Soil pH and solution pH produced similar correlations for metal arid base concentration in soil solutions. Manganese was negatively correlated with soil pH (r=- .82) and solution pH (-0.89) at Saddle Butte (Table 5.3). Iron, Al and Zn, when significant, were also negatively correlated with both soil and solution pH. Copper and Mg concentration were not correlated with pH. Calcium concentration in soil solution was positively correlated with both soil and solution pH at both Saddle Butte and Hyslop. Potassium concentration was positively correlated with soil and solution p1-I at Saddle Butte and negatively correlated with soil and solution pH at Hyslop. soil and solution pH closely agreed in predicting metal and base concentration in soil o1ution at these two locations. Soil solution P levels were affected by lime and P-application (Table 5.1, Fig. .2 and 5.3). The 4.5 Mg ha' lime application decreased P in solution at Hyslop. compared to the unlimed treatment, with a similar trend observable at Saddle Butte (Fig 5.2). Previous research at Saddle Butte showed lime application decreased NaHCO1-P (Horneck et al., 1990). Lime application at Saddle Butte did not significantly effect P. or soil test P. Lime decreased soil test P at Hyslop (Fig 5.2). Bray-PU Olsen and P all decreased when lime was applied at Hyslop. A similar trend was observed in at Saddle Butte but not for the Bray-P 1 or Olsen soil tests. This outcome was originally hypothesized to be the result of increased organic-P levels, however, Figure 5.2 shows and TP in solution were not affected by lime treatment. Lime may be supplying enough Ca and Mg to temporarily precipitate P in soil solution. This hypothesis is reinforced by the 4.5 Mg ha surface lime application having a more pronounced effect than the 9 Mg ha rate. The 9 Mg haa application equilibrated with the bulk 83 soil since it had been applied 2 years prior to sampling. The surface lime application was applied the previous fall, having only 6 months to equilibrate. Lime incorporation increases lime reaction rate (Doerge and Gardner, 1985). Phosphorous application increased P, Bray-Pl and Olsen at Hyslop and Saddle Butte. The increase in P from 0.07 mg l' to 0.2 mg t' due to P application at Hyslop is accompanied by a significant decrease in These changes result in no difference in TP. P) at Saddle Butte was not effected by P application. P for all treatments at both sites are above the Cmn established for perennial ryegrass at 0.01 mmol m3 (Breeze et al., 1984). Critical P for cool-season grasses is 25 mg P kg1 for the Bray- P1 and 15 ppm for the Olsen P soil test. soil The 2.5 cm soil samples were a poor indicator of initial P since Hyslop had 82 mg kg1 Bray-P 1 and Saddle Butte was 21 rng kg while P levels was similar at 0.075 mg 1* Bray-P 1 increased almost 40 mg kg' at Hyslop from P application, where the increase was 6 mg kg' at Saddle Butte. 1-lowever, the relative percent increase for the two sites was similar. Percent P increased to 0.2 mg l at Hyslop and 0.10 mg 11 at Saddle Butte from P application. [he larger increase at Hyslop may be due to this soil being less likely to fix P or just poorly buffered at higher Bray-P 1 than Saddle Butte. Using the assumption that a hectare 2.5 cm weighs 373,333 kg ha' and using the water content prior to extraction with a homogeneous 0.075 mg P there was 11.6 g P gP ha1 ha1 1.1 concentration. available for plant uptake as P at Saddle Butte. There was 7.0 available at Hyslop because of the lower water content. This is substantially below the daily peak demand for perennial ryegrass and tall fescue. Using data from chapter three, peak demand is 249 g P ha d1 and lasts for approximately 45 days. Estimates made using TP in solution yield 77.3 g P ha' at Saddle Butte. Since no response to P was observed these soils have to be well buffered to supply P to the root to meet demand. Correlation coefficients of Bray-P 1 and NaHCO3-P with P were r=0.59 and 0.55, at Hyslop and r=0.50 and 0.60 at Saddle Butte, respectively. Correlations indicate that conventional soil tests are linked to solution P levels at these two sites. 84 At the Hyslop site iron was correlated with P (r=-0.58). Iron reduction and oxidation may be controlling P solubility. The flux of Fe2 and Fe3 may make P available during the wet winter months. In this paper organic-P is derived by subtracting P from TP. Organic-P at Saddle Butte averaged 0.36 mg P U1, 400% of P. High levels may explain the lack f growth response from P application at this site. TP was correlated with P at Saddle Butte (r=0.53) but not at Hyslop (r=0.07). Hyslop levels averaged 0.2 118 mg P U' which was 160% of P. NaI-1CO3-P was almost twice as high as Bray-P 1 at the Saddle Butte site where as NaHCO3-P was 70% of Bray-P 1 at Hyslop. The increase in extractable P by NaHCO3 compared to Bray-P 1 at Saddle Butte may be linked to the extraction of by NaHCO3. Cleavage and hydrolization of pyrophosphate bonds may he involved. The Olsen soil test may be a better indicator of organic P availability than the Bray-P 1 and helping explain the lack of plant response to P fertilization at Saddle Butte. The lime by P interaction for (p0.05) at Saddle Butte is unexplainable and causes the same trend in TP (p=0. 1). The Lime by P interaction at Hyslop for Olsen-P result from the two lime rates decreasing Olsen-P the same. 85 Summary and Conclusions Lime application either incorporated or topdressed were both effective in increasing bulk soil and soil solution pH. Metal concentration decreased and bases increased as pH increased from lime applications. Saddle Butte had 4 times the amount of than P in the soil solution. High solution may explain the lack of plant response to P fertilization at this site. Both Saddle Butte and Hyslop had P=O.075 rng P 11 when no P was applied. NaHCO3-P, 25 mg kg1, better reflected P availability at Saddle Butte than the Bray-Pi, 12 mg kg* Phosphorous applications increased solution orthophosphate, Bray-P 1 and NaHCO3-P. Phosphorous applications had few other consistent affects in changing soil solution nutrient concentrations. 0) 0) 0.2 a, E a) 0 0.15 C0 1.2 E 4-. 0.1 LJ a) C.) 0 C'j iJ E C 0 0C 0.05 O -a w-o '7El 0.4 'j0 K7 C 00 0 C 0C 0 C) I.- 4.5 5 5.5 6 6.5 7 7.5 CN [I a) U- c pH soil Bulk oAI MnoCuvFe Zn soil in concentrations Al and Fe, Cu. Mn. Zn. on pH soil of Effect 5.1 Figure at Hyslop. solution ,12O - HYSLOP 0100 SADDLE 0 0) 80 ________ - 04E a- w60 -..-----r.-- -D Cl) 0 -c 40 -._ - _ - C 0.2 __4" s.r ct >' 0 a- Ct ci 0 0 4.5 9 0 4.5 9 LIME RATE, Mg/ha Pi BRAY-P1 LIIIPo LIIITP 0LSEN Figure 5.2 Effect of lime application on Soil test P. Hyslop and Saddle Butte. TP and P in soil solution at a, 1100 a, E I Ca) u,60 0 C', 0 40 C- ii > U [I] 0 33 0 P-rate kg/ha Figure 5.3 Effect of P application on soil test P. TP. Hyslop and Saddle Butte. 33 and P. in soil solution at Table 5.1. Analysis of variance table for the effect of lime and P application on soil solution pH and select P availability paranieters at Saddle Butte and Hyslop sites. Saddle Butte site pH Pi DF Po TP Bray Olsen Sum of squares Total 17 22.24 0.023 0.54 0.66 909 1521 Block 2 0.53 0.0047 0.018 0.031 332 423 Lime 2 14.79** 0.0013 0.046 0.035 0.78 3.11 P 1 0.63 0.0047* 0.016 0.037 288** 648** LXP 2 2.1 0.00041 0.21** 0.23* 30.3 37.3 Error 10 4.07 0.0118 0.26 0.32 258 409 pH Pi Po TP Bray Olsen Hyslop site DF Sum of squares Total 23 32.54 0.201 1.01 0.87 11346 5778 Block 3 4.23 0.0149 0.051 0.011 1580 955 Lime 2 24.87** 0.0316** 0.0076 0.0646 756** 493** P 1 0.19 0.0921** 0.148* 0.0067 7176** 3082** LXP 2 0.090 0.0148 0.121 0.072 329 434** Error 15 3.15 0.0471 0.688 0.72 1505 813 *significant at p=O.lO, **sjgnjfjcafl at p=O.O5 [able 52. Soil and solution pH at Saddle Butte and Hyslop as effected by lime treatments. Hyslop Sol* Saddle Butte Lime Soil Mgha' ------------ pH --------- 0 4.79 4.05 4.75 4.21 4.5 5.38 5.55 5.30 4.98 9.0 6.35 6.53 6.45 6.40 LSD 0.05 0.38 0.59 0.54 0.94 * Soil solution extract Soil Sol* Table 5.3. Correlation coefficients for metals in soil solution v soil solution and 1:2 pH for Hyslop and Saddle Butte sites. K Ca Mg Zn Cu Mn Fe Al r---------------------------------------------------- Saddle Butte Site Solution pH 0.5 8* * 0.86** -0.21 0.78** 0.27 0.89** 1:2 Soil pH 0.58** 0.98** -0.20 0.7O** 0.21 0.82** Solution pH 0.47** 0.86** -0.09 0.83** -0.11 O.73** 0.16 0.63** 1:2 Soil pH 0.38* 0.92** -0.01 0.82** -0.15 0.71** 0.14 0.61** -0.30 0.40* -0.09 -0.13 Flysiop Site ** significant at p=O.05, * significant at p=O.lO 92 References Adams, F., C. Burmester, N.y. Hue, and F.L Long. 1980. A comparison of columndisplacement and centrifuge methods for obtaining soil solutions. Soil Sci. Soc. Am. J. 44:733-735. Breeze. V.G., A. Wild, M. Hopper and H. Jones. 1984.The uptake of phosphate by plants from flowing nutrient solution. J. Exp. Bot. 35:1210-1221. Brady, N.C.,1984. The nature and properties of soils. 9th edition. Macmillan, New York. pp327-36l. Doerge, T. A., and H. E. 1985. Reacidification of two lime amended soils in Western Oregon. Soil Sci. Soc. Am. J. 49:680-685. 1-lart, J.M., D.A. Horneck. R.P. Dick, M. E. Mellbye. G. A. Gingrich, R.E. Costa and K.L. Wilder. 1988. Willamette valley turf type tall fescue and turf type perennial ryegrass seed nutrient survey. Soil and Water News. 3:2. Oregon state University Extension Service. Corvallis OR. F-Iillard. J.B., V.A. Haby and F.M. Hons. 1992. Annual ryegrass response to limestone and P on an Ultisol. J. Plant Nutrition. 15:1253-1268. 1-lorneck. D.A., and J.M. Hart. 1988. A survey of nutrient uptake and soil test values in perennial ryegrass and turf type tall fescue fields in the Willamette Valley. p. 13-14. In H.W. Youngberg (ed.) 1988 Seed Production Research. Dept. of Crop Science, EXT/CrS 74. Oregon State University. Corvallis, OR. Horneck. D.A., J.M. Hart. 1992. Third year response of tall fescue and perennial ryegrass to lime and phosphorus during a four year study. pp. 15-16. in W.C. Young III (ed.) 1991, Seed Production Research. Dept. of Crop Science, EXT/CrS 89. Oregon State University, Corvallis, OR. Horneck, D.A., J.M. Hart and D.C. Peek. 1990. The influence of sampling intensity, liming, P rate and method of P application on P soil test values. Communication of Soil Science and Plant Analysis. 21:13-16 pp 1079-1090. Horneck, D.A., J.M. Hart, K. Topper, and B. Koepsell. 1989. Methods of soil analysis used in the Soil Testing Laboratory at Oregon State University. SM 89:4. Agric. Exp. Sta. OSU. 21 pages. Jia. Z.J. and W.C. Dahnke. 1994. Influence of soil pH on the availability of phosphorous. Submitted to Soil Sci Soc Am. 93 Patching, W.R. 1987. Soil Survey of Lane County area, Oregon. USDA. Soil Conservation Service. 94 CHAPTER 6 FURTHER ACTION Grass seed management is currently evolving toward a system where all the straw is chopped and returned to the field. The consequences of K removal under this system are negligible. Further research needs to be conducted on the consequences of K luxury consumption in grass seed production. Do high K levels effect winter hardiness? Disease resistance? Are high K levels variety dependent with in a grass type? Several studies could accomplish this. For example, a survey of grass seed fields of the same variety for tissue K% and soil K may fill in the gaps in the model described in this paper. A growth chamber study in sand with varying K rates could accomplish similar objectives. Producers tend to manage grass seed fields as if they are annual crops instead of perennial. How fertilization benefits current years growth as well as next years, needs to be better understood. Nitrogen management and tiller development links need to be investigated. When does fertile tiller development and initiation occur in the plant? Should we be applying nitrogen at harvest to initiate next years growth, or does this stress the plant due to summer drought and decrease future seed yield? Would a summer or late spring N application break dormancy and promote vegetative growth at the cost of seed yield? These grasses may be growing during the summer just not in above ground foliage. Some '5N work looking at N efficiencies and where in the plant different N timing applications go may answer these questions. Perhaps perennial ryegrass and tall fescue are good scavengers of N because it is needed for next years growth. Looking at summer soil tests for nitrate and ammonium, N in the soil profile has either been washed away by earlier rains or been utilized by the grass seed crop. If the latter is true there are few grass seed farmers over applying N from an environmental stand point. 95 Bibliography Adams, F., C. Burmester, N.V. Hue, and F.L Long. 1980. A comparison of columndisplacement and centrifuge methods for obtaining soil solutions. Soil Sci. Soc. Am. J. 44:733-735. Bailey. J. S., 1989. Potassium-sparing effect of calcium in perennial ryegrass. J. Plant Nutrition. 12:1019-1027. Brady. N.C.,1984. The nature and properties of soils. 9th edition. Macmillan, New York. pp327-361. Breeze, V.G., A. Wild, M. Hopper and H. Jones. 1984. The uptake of phosphate by plants from flowing nutrient solution. J. Exp. Bot. 35:1210-1221. Chastain, T. and W. Young, 1994. Winter seminar. Cline, M. G. 1944. Principles of soil sampling. Soil Sci. 58:275-288. Dahl, R. C. 1977. Soil organic phosphorous.jn: N. C. Brady (ed). Advances in Agronomy. 29:83-117. International Rice Institute. Manilla, Philippines. Davidson. R.L. 1969. Effect of root/leaf temperature differentials on root/shoot ratios in some pasture grasses and clover. Ann. Bot. 33:561-569. Doerge, T. A., and H. E. 1985. Reacidification of two lime amended soils in Western Oregon. Soil Sci. Soc. Am. J. 49:680-685. Doerge, T.A., H. Gardner and T.L. Jackson. 1982. FG 6: Fine fescue seed. Oregon State Univ. Extn. Ser. Corvallis, Oregon. Doerge, T.A., H. Gardner and T.L. Jackson. 1982b. FG 44: Blue grass seed. Oregon State Univ. Extn. Ser. Corvallis. Oregon. Doerge, T.A., H. Gardner, T.L. Jackson and H. Youngberg. 1982c. FG 45: Orchardgrass seed. Oregon State Univ. Extn. Ser. Corvallis, Oregon. Doerge, T.A., H. Gardner and H. Youngberg. 1983. FG 36: Tall fescue seed. Oregon State Univ. Extn. Ser. Corvallis. Oregon. Doerge, T.A., H. Gardner T.L. Jackson. and H. Youngberg. 1982d. FG 46: Perennial ryegrass. Oregon State Univ. Extn. Ser. Corvallis, Oregon. 96 Engeistad. O.P., 1968. Use of multiple regression in fertilizer evaluation. Agron. 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Potassium effects on minerals and organic acids in three cool-season grasses. J. Plant Nutr. 15:1007-1025. Hart, J.M., D.A. Horneck, R.P. Dick, M. E. Mellbye, G. A. Gingrich, R.E. Costa and K.L. Wilder. 1988. Willamette Valley turf type tall fescue and turf type perennial ryegrass seed nutrient survey. Soil and Water News. 3:2. Oregon State University Extension Service. Corvallis, OR. Hillard, J.B., V.A. Haby and F.M. Hons. 1992. Annual ryegrass response to limestone and phosphorous on an ultisol. J. Plant Nutr. 15:1253-1268. Hooker, M. L. 1976. Sampling intensities required to estimate available N and P in five Nebraska soil types. Univ. Ne. M.S. Thesis. Horneck, D.A., and J.M. Hart. 1988. A survey of nutrient uptake and soil test values in perennial ryegrass and turf type tall fescue fields in the Willamette Valley. in: H.W. Youngberg (ed.). 1988 Seed Production Research. Dept. of Crop Science, EXT/CrS 74. p. 13-14. Oregon State University, Corvallis, OR. 97 Horneck, D.A., J.M. Hart. 1992b. Third-year response of tall fescue arid perennial ryegrass to lime and phosphorus during a four year study. pp. 6-8. j.u: W.C. Young III (ed.) 1991, Seed Production Research. Dept. of Crop Science, EXT/CrS 89. Oregon State University, Corvallis, OR. Ilorneck, D.A., J.M. Hart and D.C. Peek. 1990. The influence of sampling intensity, liming, P rate and method of P application on P soil test values. Communication of Soil Science and Plant Analysis. 21:13-16 pp. 1079-1090. Ilorneck. D. A., J. M. Hart, K. Topper, and B. Koepsell. 1989. Methods of soil analysis used in the soil testing laboratory at Oregon State University. In Press. 1-lorneck. D.A., J.M. Hart, and W.C. Young III. 1993. Uptake of N. P, K and S by five cool-season grass species. liii 992 Seed production research at Oregon State University. W.C. Young III ed. Dept. Crop and Soil Sci, Or St Univ. Ext/CrS 93. pp. 20-23. Ilorneck, D.A., J.M. Hart, W.C. Young Ill and T.B. Silberstein. 1991. Potassium for grass seed production. iii 1990 Seed production research at Oregon State University. W.C. Young III ed. Dept. Crop and Soil Sci, Or St Univ. Ext/CrS 4/91. pp. 20-23. 1-lomeck, D.A., J.M. Hart. W.C. Young III and T.B. Silberstein. 1992a. Potassium for grass seed nutrition. pp. 3-6. j: W.C. Young III (ed.) 1991. Seed Production Research. Dept. of Crop Science, EXT/CrS 80. Oregon State University, Corvallis, OR. Hulbert. L.C. 1969. Fire and litter effects in undisturbed bluestem prairie in Kansas. Ecol. 50:874-878. Jackson. T.L., E.H. Gardner, W.S. McGuire and T.E. Bedell. 1983. FG 4: Subclovergrass pasutre. Oregon State Univ. Extn. Ser. Corvallis, Oregon. Jia, Z.J. and W.C. Dahnke. 1994. Influence of soil pH on the availability of phosphorous. Submitted to Soil Sci Soc Am. Koski, A.J. 1983. Seasonal Rooting Characteristics of five cool season grasses. M.S. Thesis Ohio St. Univ. Kuhlmann, K., 1990. Importance of the subsoil for the K nutrition of crops. Plant and Soil. 127:129-136. Kunkel, R., C. D. Moore. T. S. Russell, and N. Holstad. 1971. Soil heterogeneity and potato fertilizer recommendations. Amer. Pot. J. 48:163-173. Miles, S.D. 1994. 1993 Oregon county and state agricultural estimates. Special report 790. Oregon State Univ. Extn Ser. Corvallis OR. Mitchell, K.J. 1953. Influence of light and temperature on growth of ryegrass (Lolium spp.). 1. Pattern of vegetative development. Physiologia Plantarum. 6:21-46. Orlovius. K., 1992. Potash fertilization of grassland with special consideration of K removals. Potash Review. 1:1-7. Overman. A.R. and S.R. Wilkenson. 1992. Model evaluation for perennial grasses in the Southern United States. 1992. Agron. J. 84:523-529. Overman. A.R., and S.R. Wilkenson. 1993. Modeling tall fescue cultivar response to applied nitrogen. Agron. J. 85:1156-1158. Parsons. A.J. and M. Robson. 1981. Seasonal changes in the physiology of S24 Perennial ryegrass (Lolium perenne L). 3. Partition of assimilates between root and shoot during the transition from vegetative to reproductive growth. Ann. Bot. 48:733-744. Patching. W.R. 1987. Soil Survey of Lane County area, Oregon. USDA, Soil Conservation Service. Petersen. R.G. and L.D. Calvin. 1986. Sampling. i.n: A. Klute. (ed.) Methods of soil analysis, 2nd edn. Part 1, pp. 33-52. Amer. Soc. of Agron., Madison, WI. Reed, J.F. and J.A. Rigney. 1947. Soil sampling from fields of uniform and nonuniform appearance and soil types. J. Amer. Soc. Agron. 39:26-40. Robson. M.J. 1972. The effect of temperature on the growth of S.170 fescue (Fesiuca arundinacea). J. Appl. Ecol. 9:643-653. Sabbe, W. E. and D. B. Marx. 1987. Soil sampling: spatial and temporal variability. in: J. R. Brown (ed.) Soil testing: sampling, correlation, and interpretation, pp. 1-14. Soil Sci. Soc. Amer. Madison, WI. Sheffer, K.M. and J.K. Dunn. 1981. Anatomy, drought survival and rooting depth of cool season turf grasses. Agron. Abstr. p.128. Smoliak, S. and A. Johnston. 1968. Germination and early growth of grasses at four root-zone temperatures. Can. J. Pt. Sci. 48:119-127. 99 Spiertz, J.H.J. and J. Ellen. 1972. The effect of light intensity on some morphological aspects of the crop perennial ryegrass (Lolium perenne L. var. 'Cropper") and its effect on seed production. Neth. J. Agric. Sci. 20:232-246. Stanwood. P.c. 1974. Influence of post-harvest management practices on plant growth and seed yield of cool season grasses. M.S. Thesis. Oregon St. Univ. Stuckey. 1.H. 1941. Seasonal growth of grass roots. Am. J. Bot. 28:486-491. Froughton. A. 1960. Further studies on the relationship between shoot and root systems of grasses. J. Br. Grass!. Soc. 15:41-47. Vigil, M.F.. D.E. Kissel, M.L. cabrera, c.w. Raczkowski. 1993. Optimal spacing of surface-banded nitrogen on Fescue. Soil. Sci. Soc. Am. J. 57:1629-1633. Wilkenson, S.R. and D.A. Mays. 1979. Mineral Nutrition. pp. 41-74. In R.C. Buckner and L.P. Bush (ed.) Tall Fescue. No. 20. Am. Soc. Agron. Madison, WI. Young, W. c. 1988. Personnel communication. Oregon. St.Univ. Young, w.c. 1994a. Willamette Valley grass seed production and burning data, 1986-93. Personnel communication. Oregon. St.Univ. Young, W. C.. 1994b. Seed production. In Crop and soil news/notes. 8:2. pp. 6-8. Youngberg, H.W., W.C. Young. 1990. Oregon forage and turf grass variety seed trial, 1986-87. Dept. of Crop Science, Oregon State Univ., Corvallis, OR. Ext/CrS 80. APPENDICIES 101 Appendix 1 Data from chapter 2 102 Appendix 1. Soil pH, Bray-Pi and Olsen P response to applied P. lime, sampling intensity and depth. DEPTH: 1=0-1, 2=0-6 INCHES LIME: 1=NO, 2=LIME P RATE lb/a: 1=30, 2=60, 3=90, 4=CHECK P APPLIC: l=BAND, 2=BROADCAST, 3=CHECK SAMPLE NO.: 1=5, 2=10, 3=20, 4=40, 5=50/50 DEPTH P RATE LIME P APPLC. SAMP NO. Rep 1 1 1 2 1 1 1 I 3 1 1 1 1 1 1 1 1 1 I 1 2 1 2 2 2 2 2 2 2 2 2 1 3 1 1 3 1 1 1 1 1 1 1 1 1 3 1 1 3 3 1 1 3 2 1 1 1 1 2 3 1 1 1 2 1 3 1 1 2 1 3 1 1 2 1 3 1 1 2 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 1 3 1 2 3 2 2 2 1 2 2 2 4 4 4 3 1 1 1 I 1 I 1 1 1 3 3 2 2 2 2 2 2 2 2 2 2 2 2 3 1 3 1 1 1 1 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 pH ppm 5.1 23 4.9 4.9 14 5.3 5.3 5.3 5.3 5.2 5.6 24 13 16 15 19 22 14 4.9 5.0 4.9 5.4 20 5.1 18 5.3 5.2 5.2 22 22 4.9 5.7 5.0 5.! 7.1 7.4 7.3 7.2 6.8 6.8 7.0 6.6 7.4 7.2 11 17 27 18 15 21 17 12 17 12 10 10 16 9 BRAY ppm 39 29 38 36 23 21 31 40 28 29 34 29 50 36 28 29 30 22 37 34 26 33 26 25 26 31 23 24 42 16 19 13 16 20 13 35 23 7.5 14 31 7.1 24 7.3 18 46 25 7.1 Olsen I f\ I REP DEPTH LIME 3 1 I 1 2 1 3 1 2 2 2 2 2 2 2 I 2 I 3 1 I 2 2 3 2 3 2 2 3 I 2 3 I 2 3 2 3 I 2 3 I 2 3 I 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 P RATE 3 7.1 13 4 6.8 7.2 7.7 7.2 7.2 24 20 3 4 3 4 3 4 3 3 4 4 4 4 4 1 1 I 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 1 1 1 1 2 2 2 2 2 2 2 2 2 1 3 1 1 1 3 1 1 1 3 1 1 1 3 1 3 1 3 I 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 1 1 1 1 4 4 4 1 1 1 1 1 1 1 1 3 1 3 1 3 1 2 2 2 1 2 2 2 2 2 2 2 2 2 1 3 I 1 3 1 1 3 1 1 3 2 1 3 2 1 1 4 1 1 1 1 2 2 2 3 1 1 1 SAMP NO. pH 1 1 1 P APPLC. 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 7.1 5.1 5.0 5.0 5.2 5.4 5.2 5.4 5.2 5.6 5.2 5.0 BRAY P OLSEN P ppm ppm 19 15 12 7 17 12 10 18 10 6 16 14 24 20 32 29 35 29 18 19 12 21 15 14 33 23 43 35 12 20 14 35 20 20 29 34 29 22 5.3 5.2 5.4 5.4 5.2 22 5.1 13 5.4 5.0 19 5.1 10 17 12 12 35 29 11 35 20 34 22 13 6 15 17 17 28 34 20 20 27 20 45 35 5.5 5.0 8 5.1 13 5.0 5.2 23 5.1 5.3 5.3 5.3 34 32 8 11 5.1 31 27 29 20 5.1 5.0 5.0 4.9 5.3 5.3 5.4 5.4 30 43 12 10 II 8 18 14 26 15 21 104 REP DEPTH LIME P RATE P APPLC. SAMP NO. pH BRAY P OLSEN P ppm ppm 2 2 2 2 3 2 1 4 4 4 1 2 1 2 2 3 1 2 3 2 3 1 2 1 1 3 I 2 3 2 3 I 2 3 2 3 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 5.1 9 3 2 5.3 5.0 5.0 5.0 22 2 3 2 1 1 3 1 1 1 3 1 1 1 3 1 1 1 1 1 1 2 2 2 3 5 14 26 20 22 18 1 3 5.1 1 3 5.5 7 3 5.1 12 3 3 5.0 5.0 5.2 5.0 5.5 5.2 5.3 3 1 3 1 3 1 3 1 3 1 3 1 3 1 3 1 11 16 16 1 1 10 1 3 2 2 2 3 3 4.9 14 9 19 12 9 14 12 12 20 3 23 43 29 30 34 4.9 4.8 5.0 3 1 1 12 16 10 1 3 2 2 2 1 12 5.2 5.4 5.4 3 2 2 2 2 2 2 1 29 26 30 20 31 31 23 40 27 20 21 23 21 41 4 4 3 3 5.3 3 3 5.0 14 4 3 3 5.1 11 33 23 1 1 1 1 12 1 1 1 32 28 1 1 5.0 4.9 5.0 5.3 34 1 4 4 17 1 1 1 15 1 1 5.3 5.3 5.4 1 2 2 3 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 1 1 2 2 2 2 2 2 2 2 2 2 2 2 1 3 1 1 3 1 1 3 1 1 3 1 3 1 3 2 2 2 1 4 3 1 4 3 1 1 1 I 1 1 1 1 I 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 10 18 30 21 18 6 17 16 7 12 26 20 5.8 5.0 13 31 10 5.1 23 5.0 5.4 13 22 50 28 25 22 5.1 5.6 5.1 5.3 5.2 4.9 5.3 5.0 10 14 16 12 21 16 26 35 21 27 44 33 105 REP 2 2 7 7 7 7 7 2 2 7 2 2 3 DEPTH LIME P RATE P APPLC. SAMP NO. pH ppm ppm 2 2 2 4 3 4 5.0 8 5 5.3 5.0 17 5 4.9 5.0 10 17 5 5.1 15 25 34 27 29 28 32 5 5.5 5.2 5.0 5.3 6.4 6.7 6.6 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 1 5 1 2 2 2 1 3 1 3 1 3 I 1 2 2 2 1 5 I 5 5 1 1 1 I I 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 5 1 1 I 2 2 2 2 2 2 15 10 6.7 5.7 6.6 20 8 19 6.1 6.3 12 22 10 19 6.6 6.6 6.4 6.7 II 8 24 34 24 20 6.1 27 41 6.5 6.4 6.5 6.0 6.5 6.4 6.5 6.5 6.4 6.0 6.2 6.7 6.4 6.2 5.9 6.2 6.6 16 24 15 23 IS 24 22 6.7 1 1 2 I 2 1 2 1 1 I 1 I 3 I 3 1 3 1 1 I 1 2 2 2 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 1 2 I I 1 26 6.1 I I 21 13 6.2 1 4 4 4 25 1 1 3 12 9 6.1 3 3 29 44 10 1 I 2 2 2 20 1 3 3 II 10 12 9 I 3 BRAY P OLSEN P 10 18 19 13 18 22 23 22 22 32 23 6 15 13 26 10 18 18 21 8 8 15 7 15 13 9 12 8 10 17 22 20 29 19 18 29 20 20 30 106 DEPTH LIME REP P RATE P APPLC. SAMP NO. pH BRAY P OLSEN P ppm ppm 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 2 3 I 2 3 I 2 I 2 3 2 3 2 3 I 2 2 I 2 2 3 2 3 1 4 I 2 2 2 2 2 2 2 3 1 2 3 1 2 6.4 6.5 9 9 2 2 2 2 6.1 33 2 14 2 6.3 6.3 4 4 3 2 6.1 3 2 6.2 2 4 3 2 6.1 3 3 3 14 12 10 6 22 24 49 20 22 21 20 17 2 2 2 1 1 3 1 1 3 1 I 3 2 1 2 2 2 3 I 3 6.6 6.4 6.5 6.8 5.9 6.4 6.8 I 3 6.1 10 1 3 6.4 6.0 6.4 6.7 6.6 11 16 14 9 24 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 3 3 2 2 2 2 2 2 2 2 2 3 3 1 3 3 I 3 3 3 3 3 1 3 3 3 2 2 2 3 3 3 4 4 3 3 3 3 4 3 3 1 1 1 1 1 1 1 1 1 2 2 2 4 4 4 4 4 4 4 4 4 4 2 2 2 2 2 2 2 2 2 3 1 3 1 4 4 4 3 1 4 1 1 I 4 6.1 6.3 6.1 6.4 6.4 6.5 6.3 5.9 6.5 6.5 6.5 6.5 6.1 6.0 6.6 6.0 6.4 6.2 6.5 6.6 6.8 6.4 6.3 11 23 10 20 8 18 21 7 16 8 18 24 20 32 20 II 21 21 17 29 12 22 9 21 45 60 22 15 11 18 15 23 9 6 12 9 10 7 10 20 8 21 11 9 14 9 16 22 19 22 22 22 22 36 21 16 22 11 19 21 18 32 10 10 24 17 107 REP DEPTH LIME P RATE P APPLC. SAMP NO. pH 2 2 2 2 2 2 2 3 3 2 2 2 4 3 4 4 3 3 4 4 4 4 4 4 1 I 5 BRAY P OLSEN P ppm 1 2 3 I 2 3 I 2 2 I 2 3 I 2 3 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 5 1 1 2 2 2 3 2 3 2 3 I 23 14 19 16 26 11 23 6 16 11 22 20 25 32 22 20 11 13 5 5 6.1 5 6.4 6.3 6.4 14 17 17 10 5 5 ppm 45 20 12 6.6 6.5 6.2 5 5 I 6.5 6.5 6.3 6.4 6.2 6.1 6.6 6.0 19 11 31 28 27 108 Appendix 2 from chapter 3 Dita 109 Appendix 2.1 Drymatter and nutrient concentration of seven grasses by days, heat uint, rep and grass type. Grass type 1-Tall fescu, Fawn 2-Perennial ryegrass, Linn 3-Kentucky bluegrass, Newport 4-Perennial ryegrass, Pennfine 5-Fine fescue, Pennlawn 6-Orchardgrass, Potomack 7-Tall fescue, Rebel DAYS I-lU REP GRASS DRYWT kglha N P K Ca Mg S Zn Mn Cu B % % % % % % mg/kg mg/kg mg/kg mg/kg 54 250 1 1 301 2.77 0.24 2.67 0.27 0.17 0.43 22 202 5.0 6.0 54 250 2 1 255 2.82 0.21 2.56 0.25 0.18 0.37 20 194 5.0 6.0 54 250 3 1 252 3.58 0.26 2.92 0.23 0.17 0.37 23 179 5.0 7.0 54 250 4 1 258 3 0.26 2.71 0.32 0.19 0.44 20 240 6.0 7.0 71 378 1 1 1719 3.35 0.30 3.06 0.38 0.18 0.35 21 181 6.0 1.0 71 378 2 1 1353 3.29 0.30 3.07 0.35 0.18 0.4 20 179 6.0 0.0 71 378 3 1 2539 2.89 0.28 2.93 0.35 0.17 0.38 21 195 6.0 3.0 71 378 4 1 2123 3.04 0.29 2.73 0.34 0.17 0.32 20 206 6.0 0.0 92 564 1 1 4754 2.76 0.24 3.05 0.36 0.16 0.28 14 129 5.6 6.0 92 564 2 1 4691 3.65 0.32 2.86 0.5 0.15 0.27 44 198 5.5 6.0 92 564 3 1 4728 2.82 0.25 3.05 0.36 0.16 0.3 45 149 4.9 0.0 92 564 4 1 4075 3.1 0.27 3.2 0.38 0.18 0.33 12 165 5.2 0.0 117 819 1 1 6542 1.71 0.26 3.01 0.24 0.14 (1.17 19 108 4.0 1.0 117 819 2 1 8686 1.83 0.24 2.75 0.29 0.15 0.19 50 113 3.0 0.0 117 819 3 1 8272 2.14 0.27 2.86 0.27 (1.15 0.22 13 126 3.0 0.0 117 819 4 1 9206 1.93 0.26 2.9 0.2 0.13 0.16 14 128 3.0 0.0 168 1470 1 1 9689 2.56 0.27 2.01 0.43 0.15 0.16 15 109 3.2 6.0 168 147)) 2 1 18125 1.95 0.19 1.75 0.35 0.14 0.13 10 88 3.2 6.0 168 1470 3 1 22423 2.26 0.23 1.83 0.34 0.14 0.14 11 121 3.3 8.0 168 1470 4 1 20901 1.21 0.12 1.9 0.33 0.14 0.14 11 104 3.1 5.0 71 378 1 2 104 2.73 0.26 2.24 0.52 0.15 0.3 27 201 5.0 3.0 71 378 2 2 139 3.26 0.31 2.08 (1.41 (1.16 0.23 28 240 6.0 6.0 71 378 3 2 793 1.98 0.20 1.41 0.37 0.14 0.23 26 260 4.0 3.0 71 378 4 2 472 2.61 0.27 1.61 1)39 0.13 (1.23 26 240 4.0 4.0 92 564 1 2 1315 3.27 0.28 2.67 0.5 0.15 1)27 20 170 4.5 4.0 92 564 2 2 1704 3.59 0.29 2.7 (1.59 0.17 0.3 27 155 4.9 5.0 92 564 3 2 1808 3.33 0.28 2.41 0.51 0.17 (1.27 23 260 4.0 4.0 92 564 4 2 1751 3.3 (1.31 2.14 0.47 0.15 0.27 52 250 4.0 4.0 117 819 1 2 4683 2.32 0.33 3.12 0.47 0.14 0.22 19 128 3.0 2.0 117 819 2 2 4261 2.36 0.32 2.97 0.47 0.15 0.22 22 179 3.0 4.0 117 819 3 2 3264 2.01 0.29 2.8 0.43 0.16 0.22 17 141 3.0 1.0 110 appendix 2.1. continued. DAYS FlU DRYWT N P K kg/ha % REP GRASS 4 2 5739 % % 2.07 0.29 2.44 16683 1.15 0.12 11471 0.93 0.09 2 12009 1.61 0.16 4 2 10880 0.53 1 3 665 3.82 378 2 3 653 2.83 378 3 3 721 3.17 378 4 3 854 92 564 1 3 92 564 2 3 92 564 3 92 564 4 117 819 117 117 Ca Mg S Zn Mn % Cu B % % mg/kg mg/kg 0.41 0.14 0.18 19 208 2.0 2.0 1.45 0.4 0.09 0.16 15 103 2.5 5.0 1.94 0.53 0.13 0.15 15 155 3.2 9.0 1.41 0.47 0.13 0.14 16 260 3.8 11.0 0.05 1.39 0.46 0.1 0.13 11 132 2.6 8.0 0.37 2.31 0.38 0.14 0.25 21 138 6.0 0.0 0.28 1.75 0.39 0.14 0.23 23 139 6.0 0.0 0.31 2.04 0.38 0.15 0.26 23 144 7.0 3.0 3.7 0.37 2.02 0.42 0.14 0.28 23 147 6.0 0.0 1680 2.69 0.27 2.2 0.5 0.13 0.24 30 165 5.1 1.0 1134 3.21 0.31 2.31 0.46 0.13 0.25 23 137 5.2 0.0 3 2446 3.01 0.29 2.31 0.48 0.14 0.24 23 149 5.4 0.0 3 1746 3.21 0.29 2.16 0.46 0.14 0.24 24 155 6.0 0.0 1 3 3524 2.26 0.34 2.82 (3.35 0.13 0.19 17 114 3.0 0.0 819 2 3 2618 2.47 0.37 2.71 0.33 0.12 0.2 17 124 3.0 0.0 819 3 3 4703 0.93 0.16 2.25 0.31 0.13 0.16 18 240 4.0 3.0 117 819 4 3 4340 1.3 0.20 2.84 0.36 0.12 0.19 17 131 3.0 0.0 168 1470 1 3 5964 1.22 0.19 2.16 0.5 (3.11 0.12 14 123 3.9 9.0 168 1470 2 3 4110 2.74 0.34 2.42 0.46 0.1 0.09 13 123 4.3 9.0 168 1470 3 3 7710 2.98 0.39 2.08 (1.39 0.1 0.1 12 104 3.6 7.0 168 1470 4 3 6528 3.32 0.45 2.32 0.39 0.1 0.1 13 105 4.2 8.0 171 5.0 4.0 117 819 168 1470 1 2 168 1470 2 2 168 1470 3 168 1470 71 378 71 71 71 mg/kg mg/kg 71 378 1 4 295 3.51 0.32 2.38 (1.42 0.13 0.32 28 71 378 2 4 265 3.01 0.28 2.31 1.33 0.15 0.29 28 171 5.0 4.0 71 378 3 4 704 2.58 0.27 1.8 0.34 0.14 0.22 30 260 5.0 6.0 71 378 4 4 721 2.27 (1.23 1.59 ((.38 0.15 0.23 34 380 5.0 4.0 92 564 1 4 1334 2.56 0.22 3.14 0.35 (1.18 0.29 16 136 4.8 0.0 92 564 2 4 988 3.33 0.28 2.95 0.48 (1.15 0.26 21 156 4.8 3.0 92 564 3 4 1449 3.27 0.29 2.53 0.48 0.17 0.27 27 280 4.4 5.0 92 564 4 4 1956 3.14 0.26 2.54 0.46 0.15 0.28 28 260 3.9 1.0 117 819 1 4 3292 2.48 0.33 4.4 0.5 0.16 0.24 25 192 5.0 7.0 117 819 2 4 4333 2.6 0.34 3.05 0.42 0.14 0.23 24 172 4.0 5.0 117 819 3 4 5467 2.26 0.31 2.55 0.36 0.13 0.26 22 210 3.0 5.0 117 819 4 4 3750 2.59 0.37 3.02 0.48 0.15 0.23 20 181 2.0 4.0 168 1470 1 4 8892 1.7 (1.1$ 1.58 0.48 0.11 0.14 15 130 3.1 7.0 168 1470 2 4 9618 2.3$ 0.21 1.95 (1.49 ((.13 ((.19 18 133 4.2 7.0 168 1470 3 4 11552 0.93 (1.1(1 1.47 ((.36 ((.1 0.12 11 125 2.4 8.0 168 1470 4 4 14024 1.71 0.16 2.34 ((.44 0.11 (1.19 12 11(1 3.8 8.0 54 250 1 5 62 3.67 0.25 1.57 ((.22 0.13 0.22 30 290 7.0 13.0 54 250 2 5 75 2.85 0.21 1.9 0.25 0.13 0.3 30 320 7.0 9.0 54 250 3 5 217 3.89 0.29 1.91 0.21 0.13 0.27 29 260 7.0 7.0 111 appendix 2.1. continued. DAYS HU REP GRASS DRYWF N P K % Ca Mg S % Mn Cu B mg/kg mg/kg Zn % % % % mg/kg mg/kg 54 250 4 5 480 3.38 0.26 1.88 0.22 0.14 0.26 31 290 7.0 7.0 71 378 1 5 913 2.39 0.23 1.75 0.29 0.12 0.2 25 280 5.0 3.0 71 378 2 5 1706 2.58 0.24 2.02 0.33 0.13 0.21 32 320 6.0 3.0 71 378 3 5 1711 2.51 0.26 1.65 0.32 0.14 0.2 34 350 7.0 6.0 71 378 4 5 1585 2.11 0.22 1.44 0.3 0.14 0.24 35 430 7.0 6.0 92 564 1 5 3294 3.14 0.26 2.55 0.32 0.12 0.25 32 290 6.2 0.0 92 564 2 5 2769 3.27 0.27 2.42 0.37 0.13 0.25 28 310 6.1 0.0 92 564 3 5 3082 2.76 0.23 2.08 0.34 0.13 0.21 30 330 5.8 3.0 92 564 4 5 2147 2.88 0.24 2.25 0.35 0.14 0.23 34 350 6.8 5.0 kg/ha 117 819 1 5 5066 2.62 0.34 2.55 0.27 0.12 0.19 22 260 3.0 0.0 117 819 2 5 5582 2.38 0.31 2.56 0.27 0.11 0.18 23 240 3.0 0.0 117 819 3 5 5049 1.21 0.14 2.34 0.64 0.14 0.18 25 280 3.0 1.0 117 819 4 5 3706 1.12 0.14 2.76 0.28 0.13 0.21 24 300 3.0 2.0 168 1470 1 5 9886 3.11 0.31 2.29 0.36 0.1 0.11 14 197 3.3 8.0 168 1470 2 5 9725 3.17 0.35 1.88 0.32 0.1 0.17 14 162 3.5 9.0 168 1470 3 5 8839 2.77 0.35 1.92 0.25 0.09 0.09 14 157 3.2 8.0 168 1470 4 5 10343 3.14 0.37 2.02 0.31 0(19 0.09 19 250 3.8 8.0 54 250 1 6 393 4.12 0.34 2.37 0.31 0.23 0.31 28 380 11.0 11.0 54 250 2 6 284 3.95 0.30 2.23 0.32 0.21 0.32 27 310 10.0 12.0 54 250 3 6 153 4.64 0.35 2.62 0.36 0.23 0.35 28 280 12.0 10.0 54 250 4 6 527 4.25 0.34 2.57 0.33 0.21 0.31 29 310 10.0 12.0 71 378 1 6 972 2.98 0.28 2.87 0.36 0.19 0.3 21 250 8.0 5.0 71 378 2 6 1275 3.63 0.33 2.83 0.34 0.2 0.32 22 260 8.0 5.0 71 378 3 6 659 3.51 0.38 2.88 0.3 0.19 0.28 24 350 8.0 5.0 71 378 4 6 908 3.04 0.31 2.53 0.37 0.21 0.25 25 350 9.0 6.0 92 564 1 6 2141 3.85 0.33 3.41 0.4 0.18 0.3 20 190 7.1 2.0 92 564 2 6 2220 3.97 0.37 3.51 0.43 0.19 0.31 20 174 6.5 2.0 92 564 3 6 2528 3.59 0.32 3.02 0.41 0.18 0.27 21 270 5.9 3.0 92 564 4 6 2486 3.33 0.31 3.1 0.42 0.21 0.27 20 310 7.4 4.0 117 819 1 6 6232 2.76 0.33 2.98 0.29 0.14 0.22 15 147 4.0 0.0 117 819 2 6 5015 2.56 0.27 3.11 (1.28 0.15 0.21 19 159 4.0 0.0 117 819 3 6 7354 2.44 0.3(1 3.4 0.26 0.13 0.22 15 154 3.0 0.0 117 819 4 6 5096 2.01 0.26 3.6 0.28 0.15 0.25 20 177 3.0 2.0 168 1470 1 6 9725 1.7 0.17 1.95 0.44 0.14 0.15 15 260 3.1 9.0 187 3.5 9.0 168 1470 2 6 7845 1.24 0.13 2.29 (1.44 0.16 (1.17 14 168 1470 3 6 18358 2.04 0.20 1.93 0.41 0.13 0.14 13 188 3.4 8.0 168 1470 4 6 16603 1.64 0.18 1.91 0.37 0.16 0.16 15 250 3.6 11.0 54 250 1 7 602 3.46 0.21 1.86 0.3 0.25 0.3 22 230 5.0 8.0 54 250 2 7 660 3.1 0.21 2.07 0.29 0.25 0.39 20 201 5.0 6.0 54 250 3 7 1348 2.89 0.19 1.97 (1.34 0.28 (1.33 21 195 5.0 7.0 112 appendix 2.1. continued. DAYS 1-Hi REP GRASS DRYWT kg/ha N P K % % % 54 250 4 7 571 2.97 0.19 71 378 1 7 1125 2.7 0.22 71 378 2 7 1053 3.51 71 378 3 7 1985 3.23 71 3'78 4 7 1974 92 54 1 7 2599 92 564 2 7 92 5h4 3 92 564 4 117 819 117 819 117 Ca Mg S % % % Mn Zn mg/kg mg/kg Cu B mg/kg mg/kg 1.86 0.29 0.24 0.34 18 171 5.0 6.0 2.19 0.4 0.23 0.32 20 184 5.0 0.0 0.27 2.49 0.38 0.22 0.32 20 162 5.0 1.0 0.30 2.58 0.41 0.23 0.37 23 210 6.0 3.0 2.89 0.25 2.28 0.39 0.23 0.32 23 198 5.0 2.0 2.95 0.22 2.52 0.36 0.21 0.28 17 161 5.2 3.0 2438 2.76 0.21 2.68 0.4 0.22 0.27 17 168 5.4 3.0 7 3399 3.01 0.23 2.95 0.46 0.22 0.33 15 145 4.7 0.0 7 3797 3.01 0.23 2.88 0.44 0.23 0.31 14 174 4.3 0.0 1 7 6197 2.23 0.25 3.06 0.39 0.22 0.29 17 125 4.0 0.0 2 7 5920 1.93 0.23 3.13 0.37 0.18 0.24 14 159 3.0 2.0 819 3 7 6010 1.93 0.25 2.44 0.27 0.16 0.22 15 127 3.0 1.0 117 819 4 7 5915 2.11 0.26 2.67 0.34 0.21 (1.21 14 155 4.0 2.0 168 1470 1 7 16012 1.46 0.13 1.85 0.46 0.19 (1.16 12 93 3.1 7.0 168 1471) 2 7 8579 2.26 (1.20 2.78 0.44 0.21 0.18 14 11(1 4.6 5.0 168 1470 3 7 13218 1.73 0.16 2.17 0.47 (1.2 (1.16 13 124 4.3 7.0 168 1470 4 7 18286 2.47 0.23 1.93 0.43 0.17 (1.13 10 121 3.9 7.0 113 Appendix 2.2 Nutrient uptake of seven grasses by days, heat uint, rep and grass type Grass type 1-Tall fescu, Fawn 2-Perennial ryegrass, Linn 3-Kentucky bluegrass, Newport 4-Perennial ryegrass, Penntlne 5-Fine fescue. Penniawn 6-Orchardgrass, Potomack 7-Tall fescue Rebel DAYS 1-lU REP GRASS N P K Ca kg/ha kg/ha kg/ha kg/ha Mg S kg/ha kg/ha Zn Mn Cu B kg/ha kg/ha kg/ha kg/ha 54 250 1 1 8.3 (1.7 8.0 0.8 0.5 1.3 0.007 0.061 0.002 0.002 54 250 2 1 7.2 0.5 6.5 0.6 0.5 0.9 0.005 0(149 0.001 0.002 54 250 3 1 9.0 0.7 7.4 0.6 0.4 0.9 0.006 0.045 0.001 0.002 54 250 4 1 7.7 0.7 7.0 0.8 0.5 1.1 0.005 0.062 0.002 0.002 71 378 1 1 57.6 5.2 52.6 6.5 3.1 6.0 0.036 0.311 0.010 0.002 71 378 2 1 44.5 4.1 41.5 4.7 2.4 5.4 0.027 (1.242 0.008 0.000 71 378 3 1 73.4 7.1 74.4 8.9 4.3 9.7 (1.053 0.495 0.015 0.008 71 378 4 1 64.5 6.2 57.9 7.2 3.6 6.8 0.042 0.437 0.013 0.000 92 564 1 1 131.1 11.4 145.0 17.1 7.6 13.3 0.067 0.613 0.027 0.029 92 564 2 1 171.4 15.0 134.2 23.5 7.0 12.7 0.206 0.929 0.026 0.028 92 564 3 1 133.4 11.8 144.2 17.0 7.6 14.2 (1.213 0.705 0.023 0.000 92 564 4 1 126.3 11.0 130.4 15.5 7.3 13.4 0.049 0.672 0.021 0.000 117 819 1 1 111.9 17.0 196.9 15.7 9.2 11.1 0.124 (1.707 0.026 0.007 117 819 2 1 159.0 20.8 238.9 25.2 13.0 16.5 (1.434 (1.982 0.026 0.000 117 819 3 1 177.0 22.3 236.6 22.3 12.4 18.2 0.108 1.042 0.025 0.000 117 819 4 1 177.7 23.9 267.0 18.4 12.0 14.7 0.129 1.178 0.028 0.000 168 1470 1 1 248.0 26.2 194.8 41.7 14.5 15.5 0.145 1.056 (1.031 0.058 168 147() 2 1 353.4 34.4 317.2 63.4 25.4 23.6 0.181 1.595 0.058 0.109 168 1470 3 1 506.8 51.6 410.3 76.2 31.4 31.4 0.247 2.713 0.074 0.179 168 1470 4 1 252.9 25.1 397.1 69.0 29.3 29.3 0.230 2.174 0.065 0.105 71 378 1 2 2.8 (1.3 2.3 0.5 0.2 0.3 0.003 (1.021 0.001 0.000 71 378 2 2 4.5 0.4 2.9 0.6 0.2 0.3 0.004 0.033 (1.001 0.001 71 378 3 2 15.7 1.6 11.2 2.9 1.1 1.8 (1.021 (1.206 0.003 0.002 71 378 4 2 12.3 1.3 7.6 1.8 0.6 1.1 0.012 0.113 0.002 0.002 92 564 1 2 43.0 3.7 35.1 6.6 2.0 3.5 0.026 0.223 0.006 (1.005 92 564 2 2 61.2 4.9 463) 10.1 2.9 5.1 (1.046 0.264 0.008 (1.009 92 564 3 2 60.3 5.1 43.6 9.2 3.1 4.9 0.042 (1.470 0.007 0.007 92 564 4 2 57.8 5.4 37.5 8.2 2.6 4.7 0.091 0.438 0.007 (1.007 117 819 1 2 108.6 15.5 146.1 22.0 6.6 10.3 0.089 (1.599 0.014 0.009 117 819 2 2 100.6 13.6 126.6 20.0 6.4 9.4 (1.094 (1.763 (1.013 0.017 117 819 3 2 65.6 9.5 91.4 14.0 5.2 7.2 0.055 0.46(1 (1.010 0.003 114 appendix 2.2. continued. DAYS FlU REP GRASS P N kg/ha 117 819 168 168 K kg/ha kg/ha Ca Mg S kg/ha kg/ha kg/ha Mn Cu B kg/ha kg/ha kg/ha kg/ha 10.3 0.109 1.194 0.011 0.011 66.7 15.0 26.7 0.250 1.718 0.042 0.083 60.8 14.9 17.2 0.172 1.778 0.037 0.103 169.3 56.4 15.6 16.8 0.192 3.122 0.046 0.132 5.4 151.2 50.0 10.9 14.1 0.120 1.436 0.028 0.087 2.5 15.4 2.5 0.9 1.7 0.014 0.092 0.004 0.000 18.5 1.8 11.4 2.5 0.9 1.5 0.015 0.091 0.004 0.000 22.8 2.2 14.7 2.7 1.1 1.9 0.017 0.104 0.005 0.002 4 2 118.8 16.6 140.0 23.5 1470 1 2 191.9 20.0 241.9 1470 2 2 106.7 10.3 222.5 168 1470 3 2 193.3 19.2 168 1470 4 2 57.7 71 378 1 3 25.4 71 378 2 3 71 378 3 3 8.0 Zn 71 378 4 3 31.6 3.2 17.3 3.6 1.2 2.4 0.020 0.126 0.005 0.000 92 564 1 3 45.2 4.5 37.0 8.4 2.2 4.0 0.050 0.277 0.009 0.002 92 564 2 3 36.3 3.5 26.2 5.2 1.5 2.8 0.026 0.155 0.006 0.000 92 564 3 3 73.7 7.1 56.5 11.7 3.4 5.9 0.056 0.364 0.013 0.000 92 564 4 3 56.0 5.1 37.7 8.0 2.4 4.2 0.042 0.271 0.010 0.000 117 819 1 3 79.6 12.0 99.4 12.3 4.6 6.7 1)060 0.402 0.011 0.000 117 819 2 3 64.7 9.7 70.9 8.6 3.1 5.2 0.1)45 0.325 0.008 0.00() 117 519 3 3 43.7 7.5 105.8 14.6 6.1 7.5 1)085 1.129 0.1)19 0.014 117 i9 4 3 56.4 8.7 123.3 15.6 5.2 8.2 0.074 0.569 0.013 0.000 168 1471) 1 3 72.8 11.3 128.8 29.8 6.6 7.2 0.083 1)734 0.023 1)054 168 1470 2 3 112.6 14.0 99.5 18.9 4.1 3.7 0.053 0.506 0.018 0.037 168 140 3 3 229.8 30.1 160.4 30.1 7.7 7.7 0.093 0.802 0.028 0.054 168 1470 4 3 216.7 29.4 151.5 25.5 6.5 6.5 0.085 0.685 0.027 0.052 71 378 1 4 10.4 0.9 7.0 1.2 0.4 0.9 0.008 0.050 0.001 0.001 71 378 2 4 8.0 0.7 6.1 3.5 0.4 0.8 1)007 0.045 0.001 0.001 71 378 3 4 18.2 1.9 12.7 2.4 1.0 1.5 0.021 1)183 0.004 0.004 71 378 4 4 16.4 1.7 11.5 2.7 1.1 1.7 0.025 0.274 0.004 0.003 92 5i4 1 4 34.2 2.9 41.9 4.7 2.4 3.9 0.021 0.181 0.006 0.000 92 564 2 4 32.9 2.8 29.1 4.7 1.5 2.6 0.021 1)154 0.005 1)1)03 92 564 3 4 47.4 4.2 36.6 7.0 2.5 3.9 0.039 0.406 0.006 0.007 92 564 4 4 61.4 5.1 49.7 9.0 2.9 5.5 1)055 0.509 0.008 0.002 117 819 1 4 81.6 10.9 144.8 16.5 5.3 7.9 1)082 0.632 0.016 1)023 117 819 2 4 112.7 14.7 132.2 18.2 6.! 10.0 0.11)4 0.745 0.017 0.022 117 819 3 4 123.6 16.9 139.4 19.7 7.1 14.2 1)120 1.148 0.016 0.027 117 819 4 4 97.1 13.9 113.3 18.0 5.6 8.6 0.075 0.679 0.008 0.015 168 1470 1 4 151.2 16.0 140.5 42.7 9.8 12.4 1)133 1.156 0.028 0.062 168 1470 2 4 228.9 2)).2 187.5 47.1 11.5 18.3 0.173 1.279 0.040 0.067 1.444 0.028 0.092 168 1470 3 4 107.4 11.6 169.8 41.6 11.6 13.9 1)127 168 1470 4 4 239.8 22.4 328.2 61.7 15.4 26.6 1)168 1.543 0.053 1)112 54 250 1 5 2.3 0.2 1.0 0.1 0.1 0.1 0.002 0.018 0.000 0.001 54 250 2 5 2.1 0.2 1.4 0.2 0.1 0.2 0.002 1)024 0.001 0.001 54 250 3 5 8.4 0.6 4.1 0.5 0.3 0.6 0.006 1)056 0.002 0.002 115 appendix 2.2. continued. DAYS KU REP GRASS N P K Ca kg/ha kg/ha kg/ha kg/ha 0.003 0.003 1.1 1.8 0.023 0.256 0.005 0.003 2.2 3.6 0.055 0.546 0.010 0.005 2.4 3.4 0.058 0.599 0.012 0.010 2.2 3.8 0.055 0.682 0.011 0.010 4.0 8.2 0.105 0.955 0.020 0.000 3.6 6.9 0.078 0.858 0.017 0.000 10.5 4.0 6.5 0.092 1.017 0.018 0.009 1.1 378 1 5 21.8 2.1 16.0 2.6 378 2 5 44.0 4.1 34.5 5.6 71 378 3 5 43.0 4.4 28.2 5.5 71 378 4 5 33.5 3,5 22.8 4.8 92 564 1 5 103.5 8.6 84.0 10.5 7.5 67.0 10.2 7.1 64.1 92 564 2 5 92 5t4 3 5 85.0 B kg/ba 0.139 9.0 90.5 Cu kg/ha 1.2 71 Mn 0.015 16.2 71 Zn kg/ha 1.2 5 250 S kg/ha 4 54 Mg kg/ha kg/ha 0.7 92 564 4 5 61.9 5.2 48.3 7.5 3.0 4.9 0.073 0.752 0.015 0.011 117 819 1 5 132.7 17.2 129.2 13.7 6.1 9.6 0.111 1.317 0.015 0.000 117 819 2 5 132.8 17.3 142.9 15.1 6.1 10.0 0.128 1.340 0.017 0.000 117 819 3 5 61.1 7.1 118.1 32.3 7.1 9.1 0.126 1.414 0.015 0.005 117 819 4 5 41.5 5.2 102.3 10.4 4.8 7.8 0.089 1.112 0.011 0.007 168 1470 1 5 307.5 30.6 226.4 35.6 9.9 10.9 0.138 1.948 0.033 0.079 168 1470 2 5 308.3 34.0 182.8 31.1 9.7 16.5 0.136 1.575 0.034 0.088 168 1470 3 5 244.8 30.9 169.7 22.1 8.0 8.0 0.124 1.388 0.028 0.071 168 140 4 5 324.8 38.3 208.9 32.1 9.3 9.3 0.197 2.586 0.039 0.083 54 250 1 6 26.2 1.3 9.3 1.2 0.9 1.2 0.011 0.149 0.004 0.004 54 250 2 6 11.2 0.9 6.3 0.9 0.6 0.9 0.008 0.088 0.003 0.003 54 250 3 6 7.1 0.5 4.0 0.6 0.4 0.5 0.004 0.043 0.002 0.002 54 250 4 6 22.4 1.8 13.5 1.7 1.1 1.6 0.015 0.163 0.005 0.006 71 378 1 6 29.0 2.7 27.9 3.5 1.8 2.9 0.020 0.243 0.008 0.005 71 378 2 6 46.3 4.2 .36.1 4.3 2.6 4.1 0.028 0.332 0.010 0.006 71 378 3 6 23.1 2.5 19.0 2.0 1.3 1.8 0.016 (1.231 (1.005 0.003 71 378 4 6 27.6 2.8 23(1 3.4 1.9 2.3 0.023 0.318 (1.008 0.005 92 564 1 6 82.3 7.1 73.0 8.6 3.9 6.4 0.043 0.407 0.015 0.004 92 564 2 6 88.3 8.2 77.9 9.5 4.2 6.9 0.044 0.386 0.014 0.004 92 564 3 6 9(1.8 8.1 76.4 10.4 4.6 6.8 0.053 0.683 0.015 0.008 92 564 4 6 82.9 7.7 77.1 10.4 5.2 6.7 0.050 0.771 0.018 0.010 117 819 1 6 172.() 20.6 185.7 18.1 8.7 13.7 0.093 0.916 0.025 0.000 117 819 2 6 128.4 13.5 156.0 14.0 7.5 10.5 0.095 0.797 0.020 0.000 117 819 3 6 179.4 22.1 250.0 19.1 9.6 16.2 0.11(1 1.133 0.022 0.0(X) 117 819 4 6 102.4 13.3 183.5 14.3 7.6 12.7 0.102 0.902 0.015 0.010 168 1470 1 6 165.3 16.5 189.6 42.8 13.6 14.6 0.146 2.529 0.030 0.088 168 1470 2 6 97.3 10.2 179.6 34.5 12.6 13.3 0.110 1.467 ((.027 ((.071 168 147(1 3 6 374.5 36.7 354.3 75.3 23.9 25.7 ((.239 3.451 0.062 0.147 168 1471) 4 6 272.3 20.9 317.1 61.4 26.6 26.6 ((.249 4.151 0.060 0.183 116 appendix 2.2. continued. DAYS KU REP GRASS N K 1' kg/ha kg/ha Mg S kg/ha kg/ha kg/ha Zn Mn Cu B kg/ha kg/ha kg/ha kg/ha 1.8 1.5 1.8 0.013 0.138 0.003 0.005 13.7 1.9 1.6 2.6 0.013 0.133 0.003 0.004 26.6 4.6 3.8 4.4 0.028 0.263 0.007 0.009 10.6 1.7 1.4 1.9 0.010 0.098 0.003 0.003 2.5 24.6 4.5 2.6 3.6 0.022 0.207 0.006 0.000 2.8 26.2 4.0 2.3 3.4 0.021 0.171 0.005 0.001 64.1 6.0 51.2 8.1 4.6 7.3 0.046 0.417 0.012 0.006 57.0 4.9 45.0 7.7 4.5 6.3 0.045 0.391 0.010 0.004 76.7 5.7 65.5 9.4 5.5 7.3 0.044 0.419 0.014 0.008 7 67.2 5.1 65.4 9.8 5.4 6.6 0.041 0.410 0.013 0.007 7 102.4 7.8 100.3 15.6 7.5 11.2 0.051 0.493 0.016 0.000 54 250 1 7 54 250 2 7 20.5 1.4 54 250 3 7 39.0 2.6 54 250 4 7 17.0 1.1 71 378 1 7 30.4 71 378 2 7 37.0 71 378 3 7 71 378 4 7 92 564 1 7 92 564 2 92 564 3 20.8 1.3 kg/ha Ca 11.2 92 564 4 7 114.4 8.7 109.4 16.7 8.7 11.8 0.053 0.661 0.016 0.000 117 819 1 7 138.2 15.5 189.6 24.2 13.6 18.0 0.105 0.775 0.025 0.000 117 819 2 7 114.2 13.6 185.3 21.9 10.7 14.2 0.083 (1.941 0.018 0.012 117 819 3 7 116.0 15.0 146.7 16.2 9.6 13.2 (1.090 0.763 0.018 0.006 117 819 4 7 124.8 15.4 157.9 20.1 12.4 12.4 (1.083 0.917 0.024 0.012 168 1470 1 7 233.8 20.8 296.2 73.7 30.4 25.6 0.192 1.489 0.05(1 0.112 168 1470 2 7 193.9 17.2 238.5 37.7 18.0 15.4 0.120 0.944 0.039 0.043 168 1470 3 7 228.7 21.1 286.8 62.1 26.4 21.1 0.172 1.639 0.057 0.093 168 1470 4 7 451.7 42.1 352.9 78.6 31.1 23.8 0.183 2.213 0.071 0.128 117 Appendix 2.3 Normalized nutrient uptake of seven grasses by days, heat uint, rep and grass type Grass type 1-Tall fescu, Fawn 2-Perennial Iyegrass, Lion 3-Kentucky bluegrass, Newport 4-Perennial rvegrass. Pennfine 5-Fine fescue, Pennlawn 6-Orchardgrass, Potomack 7-Tall fescue. Rebel DAYS 1-lU REP GRASS N P K Ca Mg S Zn Mn Cu B % % % % % % %, % % DRYW % 54 250 1 1 3 3 4 2 4 8 5 6 5 3 3 54 250 2 1 2 2 2 1 2 4 3 3 2 1 1 54 250 3 1 2 1 2 1 1 3 2 2 2 1 1 54 250 4 1 3 3 2 1 2 4 2 3 2 2 1 71 378 1 1 23 20 27 16 21 39 25 29 33 3 18 71 378 2 1 13 12 13 7 10 23 15 15 14 0 7 71 378 3 1 14 14 18 12 14 31 22 18 21 4 11 71 378 4 1 26 25 15 10 12 23 18 20 20 0 10 92 1 1 53 44 74 41 52 86 46 58 86 49 49 2 1 48 44 42 37 28 54 114 58 44 26 26 92 54 54 54 3 1 26 23 35 22 24 45 86 26 31 0 21 92 564 4 1 50 44 33 22 25 46 21 31 33 0 19 92 117 819 1 1 45 65 101 38 63 72 86 67 84 11 68 117 819 2 1 45 61 75 40 51 70 240 62 45 0 48 117 819 3 1 35 43 58 29 40 58 44 38 34 0 37 117 819 4 1 70 95 67 27 41 50 56 54 43 0 44 168 147(1 1 1 100 100 100 100 100 100 100 100 100 100 100 168 1470 2 1 100 100 100 100 100 100 100 100 100 100 100 168 147(1 3 1 100 100 100 1(10 100 100 100 1(8) 100 100 100 168 147(1 4 1 100 100 100 100 101) 100 100 l0() 100 100 100 71 378 1 2 1 1 1 1 1 1 1 1 1 (1 1 71 378 2 2 4 4 1 1 1 2 2 2 2 1 1 71 378 3 2 8 8 7 5 7 11 11 7 7 2 7 71 378 4 2 21 23 5 4 6 8 10 8 7 2 4 92 564 1 2 22 18 15 10 13 13 11 13 14 6 8 92 564 2 2 57 48 21 17 19 30 27 15 23 8 15 92 564 3 2 31 26 26 16 20 29 22 15 16 5 15 92 564 4 2 100 100 25 16 24 33 76 30 25 8 16 117 819 1 2 57 77 60 33 44 39 36 35 34 11 28 117 819 2 2 94 132 57 33 43 54 54 43 35 17 37 117 819 3 2 34 49 54 25 33 43 29 15 21 2 27 118 appendix 2.3. continued. DAYS HU N REP GRASS 4 2 206 % 117 819 168 1470 1 2 168 1470 2 2 168 1470 3 168 1470 71 378 71 378 71 378 71 378 92 92 P K Ca Mg S Zn % % % % % % 306 93 47 74 10) 100 100 100 100 100 100 10) 2 100 100 100 4 2 100 100 1 3 35 22 2 3 16 3 3 4 3 564 1 564 2 92 554 92 564 117 Mn Cu B % % % DRYW % 73 91 83 41 13 53 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 12 8 14 23 17 13 17 0 11 13 11 13 22 41 28 18 22 0 16 10 7 9 9 14 24 18 13 18 4 9 15 11 11 14 18 37 23 18 19 0 13 3 62 40 29 28 33 56 60 38 37 3 28 3 32 25 26 28 36 77 49 31 33 0 28 3 3 32 24 35 39 44 76 61 45 48 0 32 4 3 26 17 25 32 37 64 49 39 38 0 27 819 1 3 109 106 77 41 70 94 72 55 45 0 59 117 819 2 3 57 69 71 46 76 142 83 64 44 0 64 117 819 3 3 19 25 66 48 79 98 92 141 68 26 61 117 819 4 3 26 30 81 61 8) 126 87 83 47 0 66 168 1470 1 3 100 100 100 100 10) 100 100 100 100 100 100 168 1470 2 3 100 100 100 100 100 10) 100 100 100 100 100 168 1 470 3 3 100 10) 10) 100 100 100 100 100 100 100 100 168 147) 4 3 1 0)) 100 1 0) 100 1 0) 100 100 100 100 10) 100 71 378 1 4 7 6 5 3 4 8 6 4 5 2 3 71 378 2 4 3 4 3 7 3 4 4 4 3 2 3 71 378 3 4 17 16 7 6 9 11 17 13 13 5 6 71 378 4 4 7 7 3 4 7 6 15 18 7 3 5 92 564 1 4 23 18 30 11 25 31 16 16 23 0 15 92 564 2 4 14 14 16 10 13 14 12 12 12 4 10 92 554 3 4 44 36 22 17 21 28 31 28 23 8 92 564 4 4 26 23 15 15 19 21 33 33 14 2 117 819 1 4 54 68 103 39 54 63 62 55 6) 37 37 117 819 2 4 49 73 70 39 53 55 60 58 43 32 45 117 819 3 4 115 147 82 47 62 103 95 8)) 59 30 47 117 819 4 4 41 62 35 29 36 32 45 44 14 13 27 168 1470 1 4 100 100 100 100 100 100 100 100 10) 100 100 168 1470 2 4 10) 100 100 10) 10) 100 100 100 100 100 100 168 1470 3 4 100 100 100 100 100 100 100 100 100 100 100 168 1470 4 4 10) 100 10) 100 100 100 11)1) 10) 100 100 100 54 251) 1 5 1 1 0 C) 1 1 1 1 1 1 54 250 2 5 1 C) I 1 1 1 2 2 2 1 1 54 250 3 5 3 2 2 2 4 7 5 4 5 2 2 14 1 119 appendix 2.3. continued. DAYS REP 1-lU 54 250 71 71 GRASS N P K Ca Mg S Zn Mn % % % % % % 1( 13 14 3 9 30 6 18 43 42 15 19 28 26 28 11 15 76 49 63 0 33 42 57 54 50 0 28 81 75 73 63 13 35 53 37 29 37 13 21 17 16 22 40 30 43 47 15 24 41 30 40 76 37 50 32 378 1 5 7 7 7 7 378 2 5 14 12 19 18 71 378 3 5 18 14 17 25 71 378 4 5 10 9 11 92 5t4 1 5 34 28 37 92 564 2 5 29 22 37 33 92 564 3 5 35 23 38 47 4 5 19 13 23 23 7 % 35 11 3 % 4 23 4 % 9 8 5 5 3 13 4 DRYW B Cu 5 5 92 564 117 819 1 5 43 56 57 38 61 89 81 68 47 0 51 117 819 2 5 43 51 78 48 63 61 94 85 49 0 57 117 819 3 5 25 23 70 146 89 114 102 102 54 7 57 117 819 4 5 13 14 49 32 52 84 45 43 28 9 36 168 1470 1 5 100 100 100 100 100 100 100 100 100 100 100 168 1470 2 5 100 10(1 100 100 100 101) 100 100 100 100 100 168 1471) 3 5 l0() 100 1(X) 100 100 100 100 100 100 100 100 168 140 4 5 100 1 00 10(1 101) 101) 1(11) 100 100 100 100 100 54 250 1 6 10 8 5 3 7 8 8 6 14 5 4 8 6 10 5 4 54 U 2 6 12 4 3 5 7 7 54 250 3 6 2 1 1 1 1 2 2 1 3 1 1 54 250 4 6 8 6 4 3 4 6 6 4 9 3 3 71 313 1 6 18 16 15 8 14 20 14 10 26 6 10 71 373 2 6 48 41 20 13 20 31 26 23 37 9 16 71 378 3 6 6 7 5 3 5 7 7 7 8 2 4 71 378 4 6 10 9 7 5 7 9 9 8 14 3 5 92 564 1 6 50 43 38 20 28 44 29 16 50 5 22 92 564 2 6 91 81 43 28 34 52 40 26 53 6 28 92 564 3 6 24 22 22 14 19 27 22 20 24 5 14 92 564 4 6 30 26 24 17 20 25 20 19 31 5 15 117 819 1 6 104 124 98 42 64 94 64 36 83 0 64 117 819 2 6 132 133 87 41 6)) 79 87 54 73 0 64 117 819 3 6 48 60 71 25 40 63 46 33 35 (1 40 117 819 4 6 38 44 58 23 29 48 41 22 26 6 31 168 1470 1 6 101) 1(1(1 100 1(10 1(1(1 101) 1(10 1(11) 100 100 100 168 1470 2 6 11)1) 1(1(1 101) 1(1(1 1(1(1 100 100 100 100 100 100 168 1470 3 6 100 100 10)) 100 100 100 100 100 100 100 100 168 1470 4 6 100 10)) 100 100 1(1(1 11))) 100 100 100 100 1(1(1 54 251) 1 7 9 6 4 2 5 7 7 9 6 4 4 54 250 2 7 11 3 6 5 9 17 11 14 8 9 8 54 25)) 3 7 17 12 9 7 14 21 16 16 12 10 10 120 appendix 2.3. continued. DAYS HU REP GRASS N P % K Ca Mg S % % % % Mn Zn % % DRYW B Cu % % % 2 4 8 6 4 4 3 3 12 14 11 0 7 54 250 4 7 4 3 71 378 1 7 13 12 8 6 9 14 71 378 2 7 19 17 11 11 13 22 18 18 13 2 12 71 378 3 7 28 28 18 13 17 35 27 25 21 6 15 71 378 4 7 13 12 13 10 15 27 25 18 14 3 11 92 564 1 7 33 27 22 13 18 28 23 28 27 7 16 92 564 2 7 35 30 27 26 30 43 35 43 33 17 28 92 564 3 7 45 37 35 25 28 53 30 30 28 0 26 92 564 4 7 25 21 31 21 28 50 29 30 23 0 21 117 819 1 7 59 74 64 33 45 70 55 52 50 0 39 117 819 2 7 59 79 78 58 59 92 69 100 45 28 69 117 819 3 7 51 71 51 26 36 63 52 47 32 6 45 117 819 4 7 28 37 45 26 40 52 45 41 33 9 32 168 1470 1 7 100 100 100 100 100 100 100 100 100 100 100 168 1470 2 7 100 100 100 100 100 1(}() 100 100 100 100 100 168 1470 3 7 100 100 100 100 100 100 100 100 100 100 100 168 1470 4 7 100 100 100 100 100 100 10() 101) 100 100 100 3 121 Apendix 3 Data from chapter 4 122 Appendix 3.1. Soil test data from four K sites in chapter 4. STRAW: 1-flail chop, 2-burn FERTIliTY: 1-low, 2-high YEAR: 1-1989, 2-1990, 3-1991 LOCATION: l=pughs if, 2_-pughs pr, 3=coons if. 4=glasser pr GRASS: 1=lsll fescue, 2=perenniai ryegraes straw 1=flail chop, 2=annual burn K: 1= K added, 2=no K SOIL TEST DATA soil test data 0-2.5cm pIt YR # rep oc fert gr St K pH P ----mg/kg 1 1 1 1 1 1 1 1 6.6 soil test data (1-15 cm K 20 Ca Mg pH ------ meq/lOOg-- K P ----ingJkg---- Ca Mg --meq/lOOg-- 70 12.3 1.10 5.5 19 55 7.8 1,30 1 2 2 1 1 1 1 1 6.7 14 66 12.0 1.10 5.8 13 55 8.6 1.40 1 3 3 1 1 1 1 1 6.7 9 62 14.4 1.10 5.6 7 51 9.9 1.30 1 4 4 1 1 1 1 1 6.4 9 90 12.7 1.60 5.7 6 55 9.4 1.80 1 5 1 1 1 1 1 2 6.1 18 51 9.8 1.00 5.4 18 59 7.2 1.50 1 6 2 1 1 1 1 2 6.4 10 55 10.9 0.94 5.5 9 55 7.8 1.30 1 7 3 1 1 1 1 2 6.4 9 59 12.4 1.20 6.2 8 55 11.6 1.20 1 8 4 1 1 1 1 2 6.5 7 66 12.3 1.40 5.7 6 66 9.2 1.70 1 9 1 1 1 1 2 1 6.8 21 90 13.0 0.85 5.8 19 59 8.6 1.00 1 10 2 1 1 1 2 1 6.3 15 66 11.2 1.00 5.5 11 51 7.9 1.40 1 11 3 1 1 1 2 1 6.3 13 117 12.7 1.20 5.5 9 78 7.1 1.30 1 12 4 1 1 1 2 1 6.9 9 70 14.4 0.98 5.9 8 59 8.7 1.30 1 13 1 1 1 1 2 2 7.0 18 59 15.8 0.82 6.3 19 62 11.4 1.10 1 14 2 1 1 1 2 2 6.4 12 62 12.3 1.30 5.8 9 59 9.8 1.60 1 15 3 1 1 1 2 2 6.2 10 70 13.4 1.20 5.2 9 7(1 8.8 1.40 1 16 4 1 1 1 2 2 6.3 9 59 10.8 1.10 5.5 7 59 7.7 1.50 1 33 1 3 2 1 1 1 4.7 76 265 3.4 0.66 4.7 71 238 4.4 0.83 1 34 2 3 2 1 1 1 4.9 86 296 3.3 (1.67 4.8 73 211 3.6 0.65 1 35 3 3 2 1 1 1 5.0 120 367 3.3 0.68 5.0 104 328 3.9 0.67 1 36 4 3 2 1 1 1 4.9 133 378 2.7 0.68 4.9 124 374 0.0 0.54 1 37 1 3 2 1 1 2 4.8 80 254 3.6 0.74 4.9 73 238 4.3 0.81 1 38 2 3 2 1 1 2 4.8 110 285 3.1 0.67 4.8 77 226 3.7 0.69 1 39 3 3 2 1 1 2 4.8 120 386 3.0 0.62 5.0 104 328 3.7 0.65 1 40 4 3 2 1 1 2 5.0 142 324 3.0 0.56 4.8 113 296 2.9 0.48 1 41 1 3 2 1 2 1 4.6 75 640 4.3 0.98 4.7 64 296 4.6 0.94 1 42 2 3 2 1 2 1 4.8 111 1326 3.7 (1.84 4.7 89 538 4.1 0.79 1 43 3 3 2 1 2 1 4.8 130 1049 4.1 0.89 4.7 113 550 3.7 0.68 1 44 4 3 2 1 2 1 4.8 158 1482 2.9 0.75 4.6 130 542 2.8 0.53 1 45 1 3 2 1 2 2 4.8 86 948 4.0 0.96 4.7 66 308 4.0 0.81 1 46 2 3 2 1 2 2 4.6 100 679 4.5 1.10 4.8 86 476 4.0 0.73 123 Appendix 3.1. Continued. SOIL TEST DATA YR # soil test data 0-15 cm soil test data 0-2.5 cm pit rep bc [en gr K St p1-I P K Ca Mg pH ------ me9/100g-- ----mg/kg P K Ca Mg --meq/lOOg-- ----mg/kg---- 1 47 3 3 2 1 2 2 5.0 137 1178 3.6 0.86 4.7 101 566 3.8 0.70 1 48 4 3 2 1 2 2 4.9 167 1404 3.2 0.76 4.6 125 558 2.9 0.52 I 17 I 2 2 2 1 1 5.6 61 191 6.5 0.46 7.1 78 230 15.8 0.48 5.2 0.64 1 18 2 2 2 2 1 1 5.5 61 207 6.6 0.53 5.2 63 137 1 19 3 2 2 2 1 1 6.2 67 215 10.4 0.68 5.5 74 199 6.4 0.67 1 20 4 2 2 2 1 1 5.5 58 121 6.7 0.62 5.9 82 140 10.0 0.59 1 21 1 2 2 2 1 2 6.9 80 195 15.4 0.44 6.4 74 156 11.6 0.57 1 22 2 2 2 2 1 2 5.6 58 164 7.1 0.60 5.4 60 140 6.3 0.73 1 23 3 2 2 2 1 2 6.1 68 222 9.8 0.60 5.8 72 195 8.1 0.79 1 24 4 2 2 2 1 2 6.4 72 218 11.0 0.51 6.1 56 160 9.3 0.62 1 25 1 2 2 2 2 1 5.6 63 222 7.3 0.65 5.7 61 133 8.2 0.70 1 26 2 2 2 2 2 1 5.7 75 277 7.4 0.58 5.5 70 179 7.8 0.68 1 27 3 2 2 2 2 1 5.4 59 215 7.6 0.72 5.2 54 137 6.2 0.92 1 28 4 2 2 2 2 1 5.7 55 152 6.1 0.68 5.5 55 152 6.1 0.71 1 29 1 2 2 2 2 2 5.8 70 285 8.1 0.66 55 69 215 7.8 0.76 1 30 2 2 2 2 2 2 5.9 79 332 10.0 0.68 6.1 69 215 9.9 0.55 1 31 3 2 2 2 2 2 5.5 64 218 7.0 0.87 5.5 57 172 6.6 0.96 1 32 4 2 2 2 2 2 5.5 72 269 6.5 0.81 5.5 64 222 5.9 0.79 1 49 1 4 1 2 1 1 6.8 16 55 11.4 0.82 5.5 16 51 6.6 0.97 1 50 2 4 1 2 1 1 6.6 19 55 11.7 0.78 5.9 18 51 8.3 (1.93 1 51 3 4 1 2 1 1 6.8 26 59 12.6 1)96 5.5 28 59 7.1 1.30 1 52 4 4 1 2 I 1 7.2 29 59 17.1 0.91 6.2 29 66 10.6 1.10 1 53 1 4 1 2 1 2 7.2 14 51 15.2 0.85 5.9 14 47 6.6 0.84 1 54 2 4 1 2 1 2 6.4 7 51 10.2 0.94 6.0 18 51 8.9 1.00 1 55 3 4 1 2 1 2 6.7 27 55 12.9 0.89 5.5 30 47 7.2 1.10 1 56 4 4 1 2 1 2 6,8 31 51 17.2 1.00 5.9 30 62 8.9 1.20 1 57 1 4 1 2 2 1 7.2 27 70 15.2 0.85 5.9 20 82 9.2 0.14 1 58 2 4 1 2 2 1 7.0 26 82 12.8 0.72 5.8 20 66 8.0 1.0(1 1 59 3 4 1 2 2 1 6.6 27 78 11.2 1.10 5.9 26 90 9.0 1.20 1 61) 4 4 1 2 2 1 6.7 4! 66 12.2 1.1)) 6.)) 35 62 9.2 1.3(1 1 61 1 4 1 2 2 2 6.8 27 59 11.5 ().7 6.1 1$ 55 8,9 (1.94 1 62 2 4 1 2 2 2 6.7 26 66 11.6 0.95 5.8 22 62 79.0 1.10 1 63 3 4 1 2 2 2 6.4 32 62 11.3 1.11) 5.9 29 59 9.2 1.30 1 64 4 4 1 2 2 2 6.4 41 78 11.5 1.20 5.8 36 66 9.0 1.30 2 1 I 1 1 1 1 1 6.3 22 70 12.3 0.93 5.9 21) 43 11.6 1.01) 2 2 2 1 1 1 1 1 6.3 16 66 13.0 0.89 5.4 19 59 8.7 1.00 2 3 3 1 1 1 1 1 6.7 9 51 17.0 1.00 5.3 9 55 8.9 1.20 2 4 4 1 1 1 1 1 6.4 8 51 14.3 1.40 5.4 8 55 92 1.80 2 5 1 1 1 1 1 2 6.2 21 47 12.0 1.0)) 5.6 19 43 8.7 1.20 124 Appendix 3.1. Continued. SOIL TEST DATA YR # soil test data 0-15 cm soil test data 0-2.5 cm pit rep bc fert gr K si pH K P ----mg/kg Ca Mg pH ------ meq/I DOg-- K P Ca Mg --meq/IOOg-- ----mg/kg---- 1 1 1 2 6.4 12 55 13.9 1.10 5.6 10 47 9.7 1.30 1 1 1 1 2 6.5 8 62 14.1 1.20 5.6 7 51 11.6 1.50 4 1 1 1 1 2 6.6 8 62 15.0 1.40 5.5 7 51 9.6 1.60 9 1 1 1 1 2 1 6.7 23 70 13.0 0.92 5.9 20 74 10.6 ft95 10 2 1 1 1 2 1 6.1 16 78 11.3 1.20 56 13 66 79 1.40 11 3 1 1 1 2 1 6.1 11 109 12.3 1.30 5.6 11 74 8.6 1.40 12 4 1 1 1 2 1 6.1 12 98 12.1 1.30 5.6 7 74 8.7 1.30 2 13 1 1 1 1 2 2 6.2 22 82 10.7 1.00 5.9 23 59 9.2 1.00 2 14 2 1 1 1 2 2 6.1 11 62 12.7 1.40 5.2 ID 51 7.0 1.50 2 15 3 1 1 1 2 2 6.3 14 94 14.0 1.20 5.9 7 86 12.4 1.40 2 16 4 1 1 1 2 2 6.0 8 113 11.3 1.30 5.4 S 82 7.8 1.30 2 33 1 3 2 1 1 1 5.3 72 238 5.7 (1.68 5.3 70 226 6.0 (1.83 2 34 2 3 2 1 1 1 5.5 52 218 6.9 0.56 5.1 68 215 4.3 0.71 2 35 3 3 2 1 1 1 6.2 102 332 12.4 0.65 5.8 92 316 7.1 0.66 2 36 4 3 2 1 1 1 5.7 118 293 7.2 0.49 5.3 125 308 4.3 0.47 2 37 1 3 2 1 1 2 5.3 70 172 5.8 0.65 5.2 71 168 5.4 0.75 2 38 2 3 2 1 1 2 5.6 79 199 7.4 0.66 5.3 79 211 5.5 0.61 2 39 3 3 2 1 I 2 5,7 121 277 7.8 0.52 5.3 108 265 4.8 0.54 2 40 4 3 2 1 1 2 5.4 117 254 6.8 (1.47 5.1 117 257 3.7 (1.47 2 41 1 3 2 1 2 1 5.3 73 413 5.9 0.62 4.9 62 269 4.2 0.68 2 42 2 3 2 1 2 1 5.4 71 390 5.8 (1.66 5.5 66 347 6.4 0.74 2 43 3 3 2 1 2 1 5.4 112 562 6 0.62 4.7 89 269 3.9 0.58 2 44 4 3 2 1 2 1 5.6 122 519 7 0.52 4.8 93 335 3.3 0.46 2 45 1 3 2 1 2 2 5.1 66 335 5.5 0.65 4.9 57 230 4.4 0.73 2 46 2 3 2 1 2 2 5.4 59 491 5.5 0.65 4.9 69 335 4.1 0.66 2 47 3 3 2 1 2 2 5.7 101 488 8.6 (1.6 4.8 89 343 4.0 0.60 2 48 4 3 2 1 2 2 5.6 113 542 6 0.56 4.9 108 363 3.4 0.50 2 17 1 2 2 2 1 1 6.2 59 296 11.6 0.41 5.9 71 176 11.1 0.53 2 18 2 2 2 2 1 1 4.9 64 355 5.5 0.50 5.1 60 172 6.1 0.53 2 19 3 2 2 2 1 1 5.8 71 374 10.1 0.55 5.5 66 226 7.4 0.67 2 20 4 2 2 2 1 1 5.1 60 332 5.8 0.44 5.6 54 195 8.0 0.54 2 21 1 2 2 2 1 2 6.0 68 257 10.1 0.45 5.8 72 168 9.5 0.52 2 22 2 2 2 2 1 2 4.9 71 320 6.4 0.55 5.2 62 168 6.6 0.58 2 23 3 2 2 2 1 2 5.9 66 289 10.5 0.56 5.7 60 191 8.5 0.68 2 24 4 2 2 2 1 2 5.2 59 355 8.0 0.52 5.1 54 164 7.9 0.48 2 25 1 2 2 2 2 1 5.2 78 324 9.2 0.50 5.5 68 195 7.9 0.57 2 26 2 2 2 2 2 1 5.3 77 359 6,1) 0.72 5.4 59 187 7.7 0.72 2 27 3 2 2 2 2 1 5.2 51 289 6.9 0.6(1 5.1 48 164 7.4 0.71 2 6 2 2 7 3 2 8 2 2 2 2 1 125 Appendix 3.1. Continued. SOIL TEST DATA pit YR soil test data 0-15 cm soil test data 0-2.5 cm # rep bc gr fert K st pH P K Ca Mg pH ----mg/kg ------ meq/lOOg-- K P ----mg/kg---- Ca Mg --meq/lOOg-- 2 28 4 2 2 2 2 1 5.1 57 300 6.2 0.56 5.2 67 183 5.8 0.60 2 29 1 2 2 2 2 2 5.5 73 242 7.2 0.51 5.1 62 183 6.7 0.63 2 30 2 2 2 2 2 2 5.1 89 371 6.9 0.57 5.2 68 195 6.3 0.55 2 31 3 2 2 2 2 2 5.4 58 328 7 0.73 5.3 54 230 6.9 0.95 2 32 4 2 2 2 2 2 5.3 61 238 5.8 0.55 5.1 59 164 4.8 0.64 2 49 1 4 1 2 1 1 5.9 19 59 8.4 0.8 5.7 16 55 6.9 0.87 2 50 2 4 1 2 1 1 6.3 24 82 10.5 1.1 5.8 23 55 8.0 0.84 2 51 3 4 1 2 1 1 6.5 33 74 10.9 0.92 6.1 28 51 8.5 0.96 2 52 4 4 1 2 1 1 7 38 78 17.3 0.92 6.2 39 62 10.6 1.00 2 53 1 4 1 2 1 2 6.2 19 55 9.5 0.76 6.1 19 43 8.4 0.88 2 54 2 4 1 2 1 2 5.9 25 59 8.3 0.81 5.6 24 62 7.0 0.93 2 55 3 4 1 2 1 2 6.4 39 86 10.8 0.84 5.6 40 70 7.2 0.98 2 56 4 4 1 2 1 2 6.8 37 62 13.1 0.89 5.4 34 62 6.5 1.20 2 57 1 4 1 2 2 1 6.2 39 160 8.7 0.86 5.6 20 105 6.4 0.82 2 58 2 4 1 2 2 1 6.7 31 113 11.4 0.81 6.1 24 105 8.6 0.92 2 59 3 4 1 2 2 1 6.6 35 70 12.3 0.95 5.9 28 55 8.2 1.00 2 60 4 4 1 2 2 1 6.9 38 70 16.5 1 6.3 32 66 9.9 1.20 2 61 1 4 1 2 2 2 6.3 32 160 8.5 0.81 5.7 2(1 82 6.6 0.89 2 62 2 4 1 2 2 2 7.3 27 94 13.3 0.87 5.6 26 70 6.7 0.95 2 63 3 4 1 2 2 2 6.3 39 70 10.5 1 5.8 31 59 8.3 1.10 2 64 4 4 1 2 2 2 6.5 44 78 10.6 0.98 5.9 34 70 9.3 1.30 3 1 1 1 1 1 1 1 5.8 27 62 7.6 1.10 5.1 21 27 4.5 1.30 3 2 2 1 1 1 1 1 6.0 22 86 8.8 1.10 5.5 16 31 5.8 (1.99 3 3 3 1 1 1 1 1 5.9 14 101 11.1 1.2(1 5.7 10 51 7.8 1.10 3 4 4 1 1 1 I 6(1 tO 70 9.6 1.50 5.8 8 43 8.0 1.70 3 5 1 1 1 1 1 2 6.0 29 66 8.4 1.11) 5.6 22 35 5.7 1.10 3 6 2 1 1 1 1 2 6.2 19 51 10.9 1.10 5.5 16 31 6.6 1.1(1 3 7 3 1 1 1 1 2 6.0 13 86 1(1.1 1.40 5.3 9 39 6.5 1.30 3 8 4 1 1 1 1 2 6.1 12 62 1(1.3 1.5(1 5.5 8 43 6.7 1.60 3 9 1 1 1 1 2 1 6.3 32 94 9.8 1.10 5.7 24 47 7.0 1.00 3 10 2 1 1 1 2 1 5.6 19 137 7.0 1.4(1 5.1 14 47 4.6 1.40 3 11 3 1 1 1 2 1 5.7 19 94 8.7 1.40 5.2 12 43 4.9 1.20 3 12 4 1 1 1 2 1 5.5 13 66 7.1 1.30 5.4 9 43 5.6 1.40 1 3 13 I 1 1 1 2 2 5.8 29 78 8.1 1.10 5.6 22 31 5.1 1.00 3 14 2 1 I 1 2 2 5.7 21 74 8.1 1.60 5.2 13 43 5.8 1.40 3 15 3 1 1 1 2 2 6.2 13 82 11.2 1.10 6.1 12 55 10.2 1.10 3 16 4 1 1 2 2 5.7 16 148 7.3 1.40 5.3 11 55 6.1 1.30 3 33 1 3 2 1 1 5.3 84 382 5.6 0.65 5.0 78 293 4.7 0.73 1 1 126 Appendix 3.1. Continued. SOIL TEST DATA YR soil test data 0-15 cm soil test data 0-2.5 cm pit # rep bc feet gr st K pH P K ----mg/kg---3 34 2 3 2 1 1 1 5.2 Ca Mg pH K P Ca Mg --meq/lOOg-- ----mg/kg---- --meq/lOOg-- 85 386 5 0.57 5.2 78 277 4.9 0.68 0.58 5.1 95 293 4.6 0.61 0.43 5.3 124 343 4.9 0.45 3 35 3 3 2 1 1 1 5.4 122 503 5.1 3 36 4 3 2 1 1 1 5.7 165 495 6.6 3 37 1 3 2 1 1 2 5.1 101 445 5.1 0.67 5.1 70 222 5.1 0.79 3 38 2 3 2 1 1 2 5.3 94 382 5.1 0.59 5.2 81 238 4.7 0.65 3 39 3 3 2 1 1 2 5.3 145 519 4.7 0.54 5.2 110 281 4.9 0.58 5.2 0.51 5.2 123 293 4.4 0.53 3 40 4 3 2 1 1 2 5.6 151 449 3 41 1 3 2 1 2 1 5.3 8t 519 4.6 0.66 5.0 65 332 3.9 0.65 3 42 2 3 2 1 2 1 5.2 91 550 4.5 0.63 5.1 77 327 4.7 0.67 3 43 3 3 2 1 2 1 5.2 112 585 3.7 0.68 5.0 96 363 3.3 0.60 3 44 4 3 2 1 2 1 5.2 128 554 3.4 0.51 5.1 116 421 3.5 0.50 69 464 4 0.64 5.1 67 296 3.9 0.70 85 542 4.2 0.67 5.0 71 332 4.0 0.69 0.65 3 45 1 3 2 1 2 2 5.3 3 46 2 3 2 1 2 2 5.3 3 47 3 3 2 1 2 2 5.2 117 558 3.2 0.63 5.1 96 320 3.9 3 48 4 3 2 1 2 2 5.4 126 616 4.0 0.64 5.0 109 371 3.5 0.60 3 17 1 2 2 2 1 1 6.5 79 382 11.6 0.50 5.7 84 176 8.3 0.43 3 18 2 2 2 2 1 1 5.1 68 527 3.6 0.41 5.1 61 242 5.0 0.42 3 19 3 2 2 2 1 1 5.4 78 382 5.9 0.41 5.4 76 234 6.0 0.53 3 20 4 2 2 2 1 1 5.0 52 335 4.0 0.35 5.2 57 176 6.1 0.44 3 21 1 2 2 2 1 2 5.9 74 218 8.2 0.34 5.7 75 140 7.7 0.40 3 22 2 2 2 2 1 2 5.2 57 363 4.1 0.46 5.0 60 164 4.6 0.44 3 23 3 2 2 2 1 2 5.5 70 304 6.1 0.44 5.4 67 164 6.2 0.49 3 24 4 2 2 2 1 2 6.0 64 285 8.4 0.43 5.4 5.7 164 6.6 0.42 3 25 1 2 2 2 2 1 5.4 74 515 4.9 0.52 5.3 68 246 6.1 0.48 3 26 2 2 2 2 2 1 5.2 92 335 5.1 0.46 5.3 68 230 6.1 0.49 3 27 3 2 2 2 2 1 5.3 71 503 4.8 0.68 5.0 64 211 4.5 0.59 3 28 4 2 2 2 2 1 5.2 61 398 4.8 0.6 5.1 61 211 4.6 0.52 3 29 1 2 2 2 2 2 5.2 68 374 4.7 0.53 5.2 63 172 6.3 0.50 3 30 2 2 2 2 2 2 5.9 68 363 8.5 0.51 5.2 75 191 6.3 0.49 3 31 3 2 2 2 2 2 5.2 54 378 5.6 0.72 5.1 55 183 5.4 0.70 3 32 4 2 2 2 2 2 5.2 76 417 4.2 0.73 5.2 59 187 5.7 0.62 3 49 1 4 I 2 1 1 6.3 21 82 9.5 0.82 5.8 17 31 6.1 0.92 3 50 2 4 1 2 1 1 6.3 24 59 10.7 0.87 6.1 17 31 7.3 1.00 3 51 3 4 1 2 1 1 6.4 30 66 10.8 0.92 6.1 24 43 7.1 1.10 3 52 4 4 1 2 1 1 6.3 41 78 11.4 0.95 6.1 41) 43 8.2 1.10 3 53 1 4 1 2 1 2 6.3 18 47 IC) 0.91 5.9 15 31 6.1 0.97 3 54 2 4 1 2 1 2 6.1 27 62 7.5 1.00 5.8 22 31 5.8 (1.93 127 Appendix 3.1. Continued. SOIL TEST DATA YR # soil test data 0-15 cm soil test data 0-2.5 cm pIt rep bc fert gr st K pH K P Ca Mg pH ------ meq/lOOg-- P K Ca Mg --tneq/lOOg-- ----mg/kg---- ----mg/kg 3 55 3 4 1 2 1 2 6.0 32 47 8.8 0.87 5.6 31 27 6.1 0.90 3 56 4 4 1 2 1 2 6.6 40 70 13.7 0.96 5.7 37 39 5.6 1.20 3 57 1 4 1 2 2 1 6 34 179 7.8 I 5.8 22 86 5.8 1.00 34 144 12.7 1 6.4 22 59 6.6 0.88 1.2 6.4 32 86 7.0 1.30 3 58 2 4 1 2 2 1 6.5 3 59 3 4 1 2 2 1 6.4 36 148 6.7 3 60 4 4 1 2 2 1 6.4 52 265 10.8 1.30 6.4 37 105 8.5 1.20 3 61 1 4 1 2 2 2 6.4 28 152 7.6 0.91 6.4 16 47 6.6 1.10 3 62 2 4 1 2 2 2 6.4 28 82 9.1 1 6.2 22 43 6.9 0.95 172 9.3 1.2 6.0 32 47 6.9 1.40 66 10.5 0.97 6.1 36 39 8.1 1.00 3 63 3 4 1 2 2 2 6 45 3 64 4 4 1 2 2 2 6.3 41 128 Appendix 3.2. Straw yield components from four K sites in chapter4. STRAW: 1-flail chop, 2-burn FERTILITY: 1-low, 2-high YEAR: 1-1989, 2-1990, 3-1991 LOCATION: 1=pughs tf, 2=pughs pr, 3=coorts tf, 4=glasser pr GRASS: 1=tall fescue. 2=perennial ryegrass straw 1=flail chop, 2=annual burn K: 1= K added, 2=no K Straw at harvest straw pit YR # rep bc fert gr St K yld kg/ha K Ca Mg P TN Kup Caup Mgup Pup kg/ha kg/ha kg/ha kg/ha % % % % % 0.86 43.31 19.57 15.55 4.30 43.63 18.97 14.52 5.06 45.64 17.94 13.03 3.15 0.87 56.06 20.91 15.95 4.85 0.95 29.53 16.83 13.41 5.29 0.08 0.89 31.38 14.95 11.27 3.63 0.22 0.10 0.91 55.10 20.48 15.69 7.13 0.24 0.09 1.11 45.63 19.77 15.31 5.85 0.32 0.23 0.10 0.97 61.88 17.71 12.43 5.52 0.33 0.24 0.09 0.73 44.89 16.96 12.55 4.70 1.30 0.28 0.20 0.13 1.01 100.41 21.77 15.26 10.07 5947 1.08 0.29 0.21 0.10 0.79 64.40 17.46 12.33 5.95 6023 (1.95 0.34 0.23 0.10 0.90 57.09 20.71 13.87 6.02 2 6037 0.96 0.31 0.25 0.09 1.18 58.11 18.48 14.83 5.43 2 5717 1.16 0.33 0.23 0.09 1.05 66.60 19.06 13.02 5.15 2 2 6301 1.02 0.32 0.21 0.10 0.89 64.10 20.35 13.38 6.30 1 1 1 5238 1.88 0.27 0.17 0.08 0.89 98.74 13.95 8.85 4.19 1 1 1 5840 2.18 0.29 0.17 0.11 0.90 127.55 16.90 10.16 6.42 2 1 I I 4944 2.18 0.28 0.17 0.08 1.10 107.97 13.69 8.48 3.96 2 1 1 1 4117 2.47 0.30 0.16 0.06 0.82 101.63 12.43 6.74 2.47 3 2 1 1 2 5061 1.93 0.29 0.18 0.12 0.96 97.79 14.65 9.07 6.07 3 2 1 1 2 5362 1.96 0.27 0.17 0.07 1.03 104.88 14.28 9.06 3.75 3 3 2 1 1 2 5679 2.18 0.39 0.20 0.10 0.88 123.61 22.15 11.19 5.68 40 4 3 2 I I 2 55(8) 2.18 0.25 0.14 0.12 0.93 119.73 13.50 7.60 6.60 41 1 3 2 1 2 1 4863 1.87 0.21 0.17 (1.10 0.98 90.95 10.3() 8.09 4.86 42 2 3 2 1 2 1 3146 1.96 0.25 0.17 0.09 1.18 61.72 7.72 5.23 2.83 1 43 3 3 2 1 2 1 4036 2.10 0.24 0.15 0.07 0.91 84.69 9.82 6.09 2.83 1 44 4 3 2 1 2 1 584 2.05 0.23 0.15 0.06 0.91 11.98 1.35 0.88 0.35 1 45 1 3 2 1 2 2 3994 1.70 0.22 0.15 0.11 1.30 67.87 8.88 6.13 4.39 1 46 2 3 2 1 2 2 3086 1.81 0.19 0.13 0.09 0.75 55.75 5.76 4.11 2.78 1 1 1 6139 0.71 0.32 0.25 0.07 1 1 1 1 5618 0.78 0.34 0.26 0.09 1.08 1 1 1 1 5248 0.87 0.34 0.25 0.06 0.74 I 1 1 1 1 6926 0.81 0.30 0.23 0.07 1 1 1 1 1 2 4806 0.61 0.35 0.28 0.11 6 2 1 1 1 1 2 4542 0.69 0.33 0.25 1 7 3 1 1 1 1 2 7129 0.77 0.29 1 8 4 1 1 1 1 2 6502 0.70 0.30 1 9 1 1 1 1 2 1 5519 1.12 1 10 2 1 1 1 2 1 5217 0.86 1 11 3 1 1 1 2 1 7747 1 12 4 1 1 1 2 1 1 13 1 1 1 1 2 2 1 14 2 1 1 1 2 1 15 3 1 1 1 2 1 16 4 1 1 1 1 33 1 3 2 1 34 2 3 2 1 35 3 3 1 36 4 3 1 37 1 1 38 2 1 39 1 1 1 1 1 1 1 1 1 2 1 3 2 1 3 1 1 4 4 1 5 1 129 Appendix 3.2. Continued. Straw at harvest straw pit YR # rep bc fert gr st K yld kg/ha K Ca Mg P TN Kup Caup Mgup Pup kg/ha kg/ha kg/ha kg/ha % % % % % 0.24 0.14 0.10 0.72 76.37 9.02 5.26 3.74 1 47 3 3 2 1 2 2 3740 2.04 1 48 4 3 2 1 2 2 2461 2.10 0.26 0.14 0.11 1.03 51.73 6.30 3.53 2.71 1 17 1 2 2 2 1 1 2726 1.37 0.38 0.15 0.07 0.89 37.47 10.40 4.05 1.91 1 18 2 2 2 2 1 1 5505 1.32 0.36 0.13 0.10 0.86 72.56 19.86 7.33 5.51 1 19 3 2 2 2 1 1 6458 1.21 0.35 0.13 0.11 1.32 78.05 22.34 8.10 7.10 1.49 0.41 0.15 0.09 1.25 69.91 19.27 7.20 4.22 1.33 0.35 0.13 0.10 1.05 43.38 11.33 4.08 3.26 4.21 1.86 1 20 4 2 2 2 1 1 4688 1 21 1 2 2 2 1 2 3255 1 22 2 2 2 2 1 2 2656 1.34 0.39 0.16 0.07 0.93 35.68 10.47 1 23 3 2 2 2 1 2 5908 1.32 0.34 0.14 0.10 0.98 77.88 20.32 8.01 5.91 1 24 4 2 2 2 1 2 5587 1.31 0.36 0.14 0.08 1.09 72.92 20.27 7.58 4.47 1.19 0.31 0.11 0.08 0.84 44.79 11.69 4.24 3.01 1.25 0.32 0.12 0.11 0.93 31.82 8.12 3.00 2.80 3.81 1 25 1 2 2 2 2 1 3768 1 26 2 2 2 2 2 1 2548 1 27 3 2 2 2 2 1 3814 1.38 0.35 0.14 0.11) 0.92 52.64 13.28 5.47 1 28 4 2 2 2 2 1 3678 1.33 0.29 0.13 0.10 0.94 48.75 11)80 4.61 3.68 1 29 1 2 2 2 2 2 3621 1.18 0.31 0.10 0.11 0.97 42.58 11.09 3.80 3.98 0.31 0.12 0.07 0.90 43.73 10.57 3.98 2.37 1 30 2 2 2 2 2 2 3383 1.29 1 31 3 2 2 2 2 2 3633 1.26 0.31 0.13 0.07 0.82 45.70 11.28 4.74 2.54 1 32 4 2 2 2 2 2 4619 1.24 0.33 0.14 0.08 1.00 57.17 15.21 6.26 3.70 1 49 1 4 1 2 1 1 4855 0.91 0.38 0.19 0.10 1.07 44.08 18.33 9.44 4.86 1 50 2 4 1 2 1 1 2641 0.94 0.34 0.17 0.09 1.00 24.89 8.92 4.46 2.38 0.91 0.33 0.19 0.11) 0.95 26.34 9.67 5.42 2.90 1)88 0.36 0.18 1)06 1.03 29.86 12.04 6.22 2.03 31.75 16.16 8.28 5.47 1 51 3 4 1 2 1 1 291)1 1 52 4 4 I 2 1 1 3377 1 53 1 4 1 2 1 2 4559 0.70 0.35 0.18 0.12 1)97 1 54 2 4 1 2 1 2 3876 0.85 0.43 0.20 0.06 1)91 33.00 16.82 7.74 2.33 1 55 3 4 1 2 1 2 3083 0.91 0.38 0.23 0.11 0.93 27.99 11.77 6.94 3.39 1 56 4 4 1 2 1 2 3040 0.89 0.40 0.20 0.08 0.95 26,99 12.11 6.15 2.43 1 57 1 4 1 2 2 1 3758 1.16 0.34 0.17 0.09 1.09 43.78 12.69 6.25 3.38 0.18 0.10 0.84 31.95 10.20 5.31 2.97 0.98 47.15 12.06 6.47 2.93 1 58 2 4 1 2 2 1 2966 1.08 0.34 1 59 3 4 1 2 2 1 3664 1.2) 0.33 (1.18 0.08 1 61) 4 4 1 2 2 1 3697 1.32 1)37 0,18 0.09 1)64 48.86 13.57 6.81 3.33 1 61 1 4 1 2 2 2 3516 1J.9) 0.33 0.16 (1.11 (1.97 34.74 11.65 5.58 3.87 1 62 2 4 1 2 2 2 3157 1.04 0.35 0.18 (1.11 11.96 32.75 10.92 5.57 3.47 1.12 0.36 0.17 0.08 1.07 46.63 14.96 7.15 3.34 (1.12 1.00 38.25 12.09 6.01 4.14 1 63 3 4 1 2 2 2 4173 1 64 4 4 1 2 2 2 3451 1.11 (1.35 0.17 2 1 1 1 1 1 1 1 9304 0.74 0.32 0.20 68.85 29.77 18.61 0.00 0.42 0.28 0.14 33.78 22.52 11.26 0.00 35.11 17.55 0.00 2 2 2 1 1 1 1 1 8042 2 3 3 1 1 1 1 1 10326 0.59 0.34 0.17 60.92 2 4 4 1 1 1 1 1 7668 0.51 0.24 0.15 39.11 18.40 11.50 0.00 2 5 1 1 1 1 1 2 7258 0.43 0.29 0.16 31.21 21.05 11.61 0.00 130 Appendix 3.2. Continued. Straw at harvest straw pit YR # rep bc fert gr st K yld K Ca Mg P TN Kup Caup Mgup Pup % % kg/ha kg/ha kg/ha kg/ha % % % 2 6 2 1 1 1 1 2 8678 0.30 0.25 0.12 26.03 21.70 10.41 0.00 2 7 3 1 1 1 1 2 6968 0.35 0.31 0.17 24.39 21.60 11.85 0.00 26.95 14.37 0.00 kg/ha 2 8 4 1 1 1 1 2 8982 0.40 0.30 0.16 35.93 2 9 1 1 1 1 2 1 8850 0.88 0.27 0.15 77.88 23.90 13.28 0.00 2 10 2 1 1 1 2 1 10398 0.68 0.21 0.12 70.71 21.84 12.48 0.00 2 11 3 1 1 1 2 1 9464 0.82 0.27 0.12 77.60 25.55 11.36 0.00 2 12 4 1 1 1 2 1 8936 0.61 0.24 0.14 54.51 21.45 12.51 0.00 27.70 15.19 0.00 12.17 0.00 2 13 1 1 1 1 2 2 8934 0.80 0.31 0.17 71.47 2 14 2 1 1 1 2 2 9364 0.41 0.26 0.13 38.39 24.35 2 15 3 1 1 1 2 2 10830 0.47 0.25 0.14 50.90 27.08 15.16 0.00 2 16 4 1 1 1 2 2 9042 0.82 0.27 0.15 74.14 24.41 13.56 0.00 2 33 1 3 2 1 1 1 10046 2.24 0.31 0.16 225.03 31.14 16.07 0.00 2 34 2 3 2 1 1 1 10518 2.50 0.32 0.20 262.95 33.66 21.04 0.00 2 35 3 3 2 1 1 1 9428 2.35 0.32 0.17 221.56 30.17 16.03 0.00 2 36 4 3 2 1 1 1 8300 2.25 0.27 0.12 186.75 22.41 9.96 0.00 2 37 1 3 2 1 1 2 10776 1.85 0.26 0.13 199.36 28.02 14.01 0.00 2 38 2 3 2 I 1 2 7428 2.43 0.29 0.15 180.50 21.54 11.14 0.00 28.02 12.65 0.00 2 39 3 3 2 1 1 2 9038 2.08 0.31 0.14 187.99 2 40 4 3 2 1 1 2 9772 2.58 0.30 0.14 252.12 29.32 13.68 0.00 2 41 1 3 2 1 2 1 9816 1.57 0.24 0.12 154.11 23.56 11.78 0.00 2 42 2 3 2 1 2 1 9368 1.84 0.22 0.13 172.37 20.61 12.18 0.00 2 43 3 3 2 1 2 1 9240 1.58 0.19 0.11 145.99 17.56 10.16 0.00 2 44 4 3 2 1 2 1 7432 1.88 0.29 0.13 139.72 21.55 9.66 0.00 2 45 1 3 2 1 2 2 11368 1.76 0.20 0.11 200.08 22.74 12.50 0.00 2 46 2 3 2 1 2 2 9918 1.71 0.26 0.12 169.6() 25.79 11.90 0.00 2 47 3 3 2 1 2 2 7402 1.80 0.21 0.12 133.24 15.54 8.88 0.00 2 48 4 3 2 1 2 2 8218 1.95 0.31 0.15 160.25 25.48 12.33 0.00 2 17 1 2 2 2 1 1 5450 1.39 0.40 0.12 75.76 21.80 6.54 0.00 2 18 2 2 2 2 1 1 5192 1.40 0.46 0.11 72.69 23.88 5.71 0.00 2 19 3 2 2 2 1 1 3688 1.42 0.40 0.11 52.37 14.75 4.06 0.00 2 20 4 2 2 2 1 1 5272 1.15 0.39 0.12 60.63 20.56 6.33 0.00 2 21 2 2 2 1 2 4264 1.59 0.43 0.15 67.80 18.34 6.40 0.00 1 2 22 2 2 2 2 1 2 6018 0.84 0.38 0.09 50.55 22.87 5.42 0.00 2 23 3 2 2 2 1 2 3768 1.58 0.52 0.13 59.53 19.59 4.9(3 0.00 2 24 4 2 2 2 1 2 3398 1.28 0.40 0.13 43.49 13.59 4.42 0.00 0.12 2 25 1 2 2 2 2 1 6752 1.60 0.49 108.03 33.08 8.10 0.00 2 26 2 2 2 2 2 1 5090 1.20 0.33 0.09 61.1)8 16.80 4.58 0.00 2 27 3 2 2 2 2 1 6452 1.50 0.52 0.13 96.78 33.55 8.39 0.00 131 Appendix 3.2. Continued. Straw at harvest pit YR straw # rep bc fert gr St K yld K kg/ha Ca Mg P TN Kup Caup Mgup Pup % % kg/ha kg/ha kg/ha kg/ha % % % 4 2 2 2 2 1 6032 1.19 0.32 0.10 71.78 19.30 6.03 0.00 29 1 2 2 2 2 2 5970 1.31 0.37 0.11 78.21 22.09 6.57 0.00 30 2 2 2 2 2 2 4756 1.15 0.38 0.08 54.69 18.07 3.80 0.00 2 31 3 2 2 2 2 2 6440 1.37 0.35 0.11 88.23 22.54 7.08 0.00 2 32 4 2 2 2 2 2 5858 1.27 0.28 0.09 74.40 16.40 5.27 0.00 2 49 1 4 1 2 1 1 5810 0.64 0.46 0.15 37.18 26.73 8.72 0.00 2 50 2 4 1 2 1 1 7284 0.73 0.42 0.13 53.17 30.59 9.47 0.00 2 51 3 4 1 2 1 1 6810 0.71 0.46 0.15 48.35 31.33 10.22 0.00 2 52 4 4 1 2 1 1 6312 0.82 (1.52 0.14 51.76 32.82 8.84 0.00 2 53 1 4 1 2 1 2 7290 0.72 0.35 0.15 52.49 25.52 10.94 0.00 2 54 2 4 1 2 1 2 6456 0.00 0.00 0.00 0.00 2 55 3 4 1 2 1 2 6378 0.44 0.31 0.12 28.06 19.77 7.65 0.00 2 56 4 4 1 2 1 2 5852 0.58 0.38 0.11 33.94 22.24 6.44 0.00 2 57 1 4 1 2 2 1 8046 1.06 0.38 0.13 85.31 30.58 10.46 0.00 2 58 2 4 1 2 2 1 8684 0.86 0.32 0.11 74.68 27.79 9.55 0.00 2 59 3 4 1 2 2 1 8536 1.33 0.27 0.12 113.53 23.05 10.24 0.00 2 28 2 2 2 60 4 4 1 2 2 1 6888 1.25 0.38 0.14 86.10 26.17 9.64 0.00 2 61 1 4 1 2 2 2 6984 0.78 0.27 0.09 54.48 18.86 6.29 0.00 2 62 2 4 1 2 2 2 8898 0.49 0.31 0.09 43.60 27.58 8.01 0.00 2 63 3 4 1 2 2 2 7828 0.59 0.41 (1.11 46.19 32.09 8.61 0.00 2 64 4 4 1 2 2 2 7368 0.82 (1.25 0.08 60.42 18.42 5.89 0.00 3 1 1 1 1 1 1 1 9172 0.67 0.27 0.16 0.08 61.45 24.76 14.68 7.34 3 2 2 1 1 1 1 1 7001) 0.71 0.33 0.17 0.09 49.70 23.10 11.90 6.30 3 3 3 1 1 1 1 1 8647 0.00 0.00 0.00 0.00 3 4 4 1 1 1 1 1 9784 0.59 0.44 0.23 0.11 57.73 43.05 22.50 10.76 3 5 1 1 1 1 1 2 9925 0.42 0.32 0.18 0.09 41.69 31.76 17.87 8.93 3 6 2 1 1 1 1 2 9026 0.46 0.34 0.18 0.10 41.52 30.69 16.25 9.03 3 7 3 1 1 1 1 2 9744 0.00 0.00 0.00 0.00 3 8 4 1 1 1 1 2 8825 0.46 0.48 0.22 0.12 40.60 42.36 19.42 10.59 3 9 1 1 1 1 2 1 7401 0.86 0.30 0.15 0.10 63.65 22.20 11.10 7.40 3 10 2 1 1 1 2 1 7400 0.85 0.39 (1.18 0.10 62.90 28.86 13.32 7.40 3 11 3 1 1 1 2 3 12 4 1 1 1 2 1 1 1 2 1 8483 1.07 0.44 0.19 0.11 90.77 37.33 16.12 9.33 1 7982 0.78 0.40 0.18 0.10 62.26 31.93 14.37 7.98 2 9172 0.62 0.35 0.17 0.11 56.86 32.10 15.59 10.09 3 13 1 3 14 2 1 1 1 2 2 9611 0.00 0.00 0.00 0.00 3 15 3 1 1 1 2 2 10195 0.73 0.33 0.15 0.10 74.43 33.64 15.29 10.20 3 16 4 1 1 1 2 2 9222 0.92 0.31 (1.17 0.10 84.84 28.59 15.68 9.22 3 33 3 2 1 1 1 13012 2.80 0.30 0.18 0.20 364.35 39.04 23.42 26.02 1 132 Appendix 3.2. Continued. Straw at harvest pit YR straw # rep bc feri gr st K yid K Ca Mg P TN Kup Caup Mgup Pup % kg/ha kg/ha kg/ha kg/ha kg/ha % % % % 3 34 2 3 2 1 1 1 12192 2.09 0.20 0.14 0.10 254.80 24.38 3 35 3 3 2 1 1 1 11414 3.08 0.26 0.17 0.21 351.55 3 36 4 3 2 1 1 1 10509 2.77 0.31 0.16 0.19 291.09 3 37 1 3 2 1 1 2 12094 1.99 0.29 0.17 0.15 3 38 2 3 2 1 1 2 10874 2.85 0.33 0.22 0.18 3 39 3 3 2 1 1 2 11073 2.41 0.31 0.19 3 40 4 3 2 1 1 2 8929 2.49 0.39 3 41 1 3 2 1 2 1 9825 2.16 0.26 3 42 2 3 2 1 2 1 9869 1.68 0.20 17.07 12.19 29.68 19.40 23.97 32.58 16.81 19.97 240.67 35.07 20.56 18.14 309.91 35.88 23.92 19.57 0.19 266.85 34.33 21.04 21.04 0.18 0.18 222.33 34.82 16.07 16.07 0.21 0.15 212.23 25.55 20.63 14.74 0.12 0.09 165.80 19.74 11.84 8.88 3 43 3 3 2 1 2 1 9201 2.41 0.19 0.11 0.12 221.75 17.48 10.12 11.04 3 44 4 3 2 1 2 1 8027 2.71 0.27 0.14 0.16 217.52 21.67 11.24 12.84 3 45 1 3 2 1 2 2 9197 2.52 0.22 0.13 0.15 231.77 20.23 11.96 13.80 3 46 2 3 2 1 2 2 10359 2.13 0.22 0.12 0.10 220.65 22.79 12.43 10.36 3 47 3 3 2 1 2 2 10811 2.20 0.17 0.11 0.13 237.84 18.38 11.89 14.05 3 48 4 3 2 1 2 2 8669 2.24 0.32 0.16 0.14 194.19 27.74 13.87 12.14 3 17 1 2 2 2 1 1 8387 1.49 0.38 0.10 0.16 124.97 31.87 8.39 13.42 3 18 2 2 2 2 1 1 6582 1.35 0.27 0.08 0.12 88.86 17.77 5.27 7.90 3 19 3 2 2 2 1 1 8328 1.27 0.32 0.10 0.13 105.77 26.65 8.33 10.83 3 20 4 2 2 2 1 1 8368 1.68 0.40 0.12 0.17 140.59 33.47 10.04 14.23 3 21 1 2 2 2 1 2 7629 1.39 0.38 0.10 0.16 106.04 28.99 7.63 12.21 3 22 2 2 2 2 1 2 6388 1.28 0.28 0.09 0.12 81.77 17.89 5.75 7.67 3 23 3 2 2 2 1 2 7961 1.38 0.31 0.10 0.13 109.86 24.68 7.96 10.35 3 24 4 2 2 2 1 2 7540 1.51 0.38 0.11 0.15 113.86 28.65 8.29 11.31 3 25 1 2 2 2 2 1 7106 1.23 0.38 0.10 0.12 87.41 27.00 7.11 8.53 3 26 2 2 2 2 2 1 6991 1.25 0.30 0.08 0.12 87.38 20.97 5.59 8.39 3 27 3 2 2 2 2 1 6602 1.27 0.38 0.11 0.13 83.84 25.09 7.26 8.58 3 28 4 2 2 2 2 1 6372 1.29 0.25 0.08 0.10 82.19 15.93 5.10 6.37 3 29 1 2 2 2 2 2 6682 1.27 0.29 0.09 0.11 84.86 19.38 6.01 7.35 3 30 2 2 2 2 2 2 7245 1.24 0.30 0.08 0.11 89.84 21.74 5.80 7.97 3 31 3 2 2 2 2 2 6473 1.45 0.31 0.10 0.14 93.86 20.07 6.47 9.06 3 32 4 2 2 2 2 2 7498 1.30 0.27 0.09 0.12 97.48 20.24 6.75 9.00 3 49 1 4 1 2 1 1 7165 0.49 0.36 0.11 0.07 35.11 25.79 7.88 5.02 3 50 2 4 1 2 1 1 7222 0.53 0.31 0.10 0.08 38.28 22.39 7.22 5.78 3 51 3 4 1 2 1 1 5713 0.52 0.36 0.11 0.09 29.71 20.57 6.28 5.14 3 52 4 4 1 2 1 I 6616 0.51 0.37 0.11 0.09 33.74 24.48 7.28 5.95 3 53 1 4 1 2 1 2 6765 (L3() 0.46 0.12 (1.09 20.3() 31.12 8.12 6.09 3 54 2 4 1 2 1 2 5484 (1.4(1 (1.43 (1.13 (3. 11) 21.94 23.58 7.13 5.48 133 Appendix 3.2. Continued. Straw at harvest pit Straw YR # rep bc fert gr St K yld kgjha P TN Kup Caup Mgup Pup % % % % % kg/ha kg/ha kgjha kg/ha K Ca Mg 3 55 3 4 1 2 1 2 6246 0.45 0.41 0.13 0.11 28.11 25.61 8.12 6.87 3 56 4 4 1 2 1 2 6497 0.41 0.34 0.12 0.11 26.37 22.35 7.56 6.84 3 57 1 4 1 2 2 1 7076 0.81 0.22 0.08 0.07 57.09 15.78 5.74 4.85 3 58 2 4 1 2 2 1 7145 0.72 0.24 0.09 0.08 51.23 16.84 6.10 5.40 3 59 3 4 1 2 2 1 6940 0.91 0.24 0.08 0.07 63.11 16.44 5.49 5.10 3 60 4 4 1 2 2 1 6974 1.11 0.22 0.08 0.10 77.64 15.54 5.46 6.67 3 61 1 4 t 2 2 2 7979 1.00 0.20 0.08 0.07 79.44 15.82 6.72 5.60 3 62 2 4 1 2 2 2 6674 0.53 0.22 0.07 0.09 35.60 t4.87 4.68 5.86 3 63 3 4 1 2 2 2 8042 0.89 0.20 0.09 0.09 71.74 15.70 7.01 7.04 3 64 4 4 1 2 2 2 7264 0.69 0.24 0.08 (1.08 50.42 17.36 5.62 5.86 134 Appendix 3.3. Seed yield components from four K sites in chapter 4. STRAW: 1-flail chop, 2-burn FERT1UTY: 1-tow, 2-high YEAR: 1-1989, 2-1990, 3-1991 LOCATION: 1rpughs if, 2=pughs pr, 3=coons If, 4=glasser pr GRASS: 1=tatI fescue, 2=perennial ryegrass straw 1=flail chop, 2=annual burn K: 1= K added, 2=no K seed pit YR # rep ac fert gr si K yld kg/ha SEED SEED K TP TN Ca % % % % 2.40 0,17 Mg % Kup Pup kg/ha kg/ha 0.16 8.16 Nup kg/ha Caup kg/ha 6.40 38.40 2.72 1 1 1 1 1 1 1 1 1600 0.51 0.40 1 2 2 1 1 1 1 1 1147 0.53 0.35 2.19 0,19 0.17 6.08 4.01 25,12 2,18 5.21 4.05 27.68 1.85 1 3 3 1 1 1 1 1 1158 0.45 0.35 239 0.16 0.17 1 4 4 1 1 1 1 1 1274 0.46 0.36 2.29 0.26 0.17 5.86 4.59 29.17 3.31 1 5 1 1 1 1 1 2 934 0.42 0.37 2.34 0.20 0.14 3.92 3.46 21.86 1.87 1 6 2 1 1 1 1 2 1044 0.43 0.34 2.23 0.18 0.16 4.49 3.55 23.28 1.88 1 7 .1 1 1 1 1 2 1481 0.44 0.36 2.42 0.19 0.15 6.52 5.33 35.84 2.81 6.05 4.89 29.75 3.35 5.03 3.77 25.03 2.17 1.55 1 8 4 1 I 1 1 2 1288 ((47 0.38 2.31 0.26 0.15 1 9 1 I 1 1 2 1 1143 0,44 0.33 2.19 0.19 (1.14 1 10 2 1 1 1 2 1 1035 0.42 0.34 2.32 0.15 0.12 4.35 3.52 24,01 1 11 3 1 1 1 2 1 1632 0.45 0.36 2.23 0.20 0.12 7.34 5.88 36.39 3.26 1 12 4 1 1 1 2 1 1382 0.45 0.34 2.18 0.13 0.12 6.22 4.70 30.13 1.80 1 13 1 1 1 1 2 2 1101 0.45 0.34 2.25 0.18 (1.15 4.95 3.74 24.77 1.98 1 14 2 1 1 1 2 2 933 0.47 0.36 2.42 0.22 0.16 4.39 3.36 22.58 2.05 28.32 2.51 1 15 3 1 1 1 2 2 1253 (1.51 0.35 2.26 (1.20 (1.16 6.39 4.39 1 16 4 1 1 1 2 2 1386 (1.53 0.34 2.08 0.17 0.13 7.35 4.71 28.83 2.36 1 33 I 3 2 I 1 1219 0.46 0.35 2.46 0.26 (1.15 5.61 4.27 29.99 3.17 (1.14 (I. 12 7.32 5.73 40.25 2.23 0.24 (1.15 5.31 3.98 28.82 2.89 5.81 4.04 31.98 1.90 1 34 2 3 2 1 35 3 3 2 1 1 1 1 1591 0.46 0.36 2.53 1 1 1206 0.44 0.33 2.39 1 36 4 3 2 1 1 1 1264 0.46 0.32 2.53 0.15 0.14 1 37 1 3 2 1 1 2 1448 0.47 0.34 2.42 0.17 0.16 6.81 4.92 35.04 2.46 1 38 2 3 2 1 1 2 1198 0.46 0.37 2.84 0.21 0.16 5.51 4.43 34.02 2.52 1 39 3 3 2 1 1 2 1445 0.46 0.39 2.93 0.2(1 0.15 6.65 5.64 42.34 2.89 1 40 4 3 2 1 1 2 1162 0.47 0.33 2.58 0.15 0.14 5.46 3.83 29.98 1.74 (1.15 3.04 2.36 16.27 1.30 1.96 1 41 1 3 2 1 2 1 621 0.49 0.38 2.62 (1.21 1 42 2 3 2 1 2 I 851 (1.47 (1.34 2.47 (1.23 (1.15 4.00 2.89 21.02 1 43 .5 3 2 1 2 I 1089 0.42 0.29 2.26 0.17 (1.15 4.57 3.16 24.61 1.85 1.04 0.73 5.55 0.38 4 3 2 1 2 1 236 0.44 (1.31 2.35 0.16 (1.15 45 1 3 2 1 2 2 618 (1.49 0.34 2.36 0.19 (1.16 3.03 2.10 14.58 1.17 46 2 3 2 1 2 2 758 0.48 0.35 2.36 0.15 0.13 3.64 2.65 17.89 1.14 1 44 1 1 135 Appendix 3.3. Continued. seed pit YR # rep bc fert gr K st yld kg/ha SEED SEED K TP TN Ca Mg Kup Pup Nup Caup 1 47 3 3 2 1 2 2 872 0.53 0.29 2.33 0.14 0.12 4.62 2.53 20.32 1.22 1 48 4 3 2 1 2 2 665 0.45 0.29 2.40 0.20 0.14 2.99 1.93 15.96 1.33 1 17 1 2 2 2 1 1 1220 0.51 0.34 2.03 0.17 0.16 6.22 4.15 24.77 2.07 1 18 2 2 2 2 1 1 1721 0.48 0.34 2.10 0.23 0.13 8.26 5.85 36.14 3.96 1 19 3 2 2 2 1 1 1486 0.48 0.33 2.04 0.21 0.17 7.13 4.90 30.31 3.12 1 20 4 2 2 2 1 1 1872 0.51 0.38 2.39 0.19 0.15 9.55 7.11 44.74 3.56 1 21 1 2 2 2 1 2 1357 0.52 0.34 2.10 0.18 0.14 7.06 4.61 28.50 2.44 1 22 2 2 2 2 1 2 1854 0.49 0.34 2.17 0.19 0.17 '3.08 6.30 40.23 3.52 1 23 3 2 2 2 1 2 1779 0.46 0.36 2.22 0.16 ((.14 8.18 6.40 39,49 2.85 1 24 4 2 2 2 1 2 1332 0.49 0.37 2.16 0.20 0.15 6.53 4,93 28.77 2.66 1 25 1 2 2 2 2 1 1357 0.42 0.35 2.12 0.19 0.14 5.70 4.75 28.77 2.58 1 26 2 2 2 2 2 1 1398 0.47 0.37 2.17 0.16 0.14 6.57 5.17 30.34 2.24 1 27 3 2 2 2 2 1 2182 0.55 0.35 2.16 0.18 ((.15 12.01) 7.64 47.13 3.93 1 28 4 2 2 2 2 1 1703 0.51 0.38 2.15 0.21 0.12 8.69 6.47 36.61 3.58 1 29 1 2 2 2 2 2 1555 0.47 0.35 2.07 0.18 (1.13 7.31 5.44 32.19 2.80 1 30 2 2 2 2 2 2 1229 0.52 0.34 2.01 0.24 0.15 6.39 4.18 24.70 2.95 1 31 3 2 2 2 2 2 2107 (1.47 0.34 1.99 0.16 0.16 9.9(1 7.16 41.93 3.37 1 32 4 2 2 2 2 2 1531 0.50 0.36 2.05 0.19 0.16 7.66 5.51 31.39 2.91 1 49 1 4 1 2 1 1 1910 (>44 0.34 2.32 0.21 0.17 8.4(1 6.49 44.31 4.01 1 50 2 4 1 2 1 1 1049 0.41 0.32 2.11 0.21 0.16 4.30 3.36 22.13 2.20 1 51 3 4 1 2 1 1 1045 (1,44 0.29 2.07 0.17 0.14 4.60 3.03 21.63 1.78 1 52 4 4 1 2 1 1 1030 (>42 0.35 2.26 0.16 0.15 4.33 3.61 23.28 1.65 1 53 1 4 1 2 1 2 1335 0.40 0.35 2.35 0.14 (1,13 5.34 4.67 31.37 1.87 1 54 2 4 1 2 1 2 1454 0.40 0.33 2.15 0.16 (1.14 5.82 4.80 31.26 2.33 1 55 3 4 1 2 1 2 1273 0.46 0.32 2.23 0.18 (1.15 5.86 4.07 28.39 2.29 1 56 4 4 1 2 1 2 1111 0.44 0.36 2.28 0.24 0.16 4.89 4.00 25.33 2.67 1 57 1 4 1 2 2 1 1264 0.44 0.32 2.19 0.25 0.16 5.56 4.04 27.68 3.16 1 58 2 4 1 2 2 1 878 0.42 0.32 2.16 0.14 0.13 3.69 2.81 18.96 1.23 1 59 3 4 1 2 2 1 1410 0.51 0.38 2.30 0.21 0.15 7.19 5.36 32.43 2.96 1 60 4 4 1 2 2 1 1223 (1.47 0.37 2.29 0.15 ((.13 5.75 4,53 28.01 1.83 1 61 1 4 I 2 2 2 943 (3.46 0.36 2.29 0.20 0.14 4.34 3.39 21.59 1.89 1 62 2 4 1 2 2 2 1148 0.45 0.39 2.33 (1.19 0.15 5.17 4.48 26.75 2.18 1 63 3 4 1 2 2 2 1516 0.44 0.37 2.28 0.21 0.15 6.67 5.61 34.56 3.18 1 64 4 4 1 2 2 2 1264 0.43 (1.36 2.25 0.18 (1.14 5.44 4.55 28.44 2.28 1 1585 (>62 0.34 2.09 9.83 5.39 33.13 1546 0.61 0.34 2.15 9,43 5.26 33.24 1511 0.63 0.33 2.04 9.52 4.99 30.82 1314 0.62 0.39 2.25 8.15 5.12 29.57 211 1111 22211111 23311111 24411111 25111112 1374 136 Appendix 3.3. Continued. seed p0 YR # rep bc fert gr K sE yld kg/ha 26211112 27311112 28411112 29111121 2 1 1347 2 2 1512 1 2 2 1369 1 1 2 2 1506 1 1 2 2 1392 1 1899 1 1 2 I 1 1 2 2 12 4 1 1 1 2 13 1 1 1 1 2 14 2 1 1 2 15 3 1 2 16 4 1 Ca Mg Kup Pup Nup 1487 1522 1 3 TN 1402 1642 2 11 TP 1416 I 10 2 K SEED 1509 1 2 SEED 0.00 0.00 0.00 2339 0.61 0.33 2.lf 14.27 7.72 50.52 1969 0.62 0.35 2.24 12.21 6.89 44.11 1 1997 0.59 0.35 2.1 11.78 6,99 43.33 2 2022 0.59 0.33 2.0 11.93 6.67 42.26 2 33 1 3 2 1 1 2 34 2 3 2 1 1 2 35 3 3 2 1 1 2 36 4 3 2 1 1 2 37 1 3 2 1 1 2 38 2 3 2 1 1 2 1611 2 39 3 3 2 1 1 2 2011 2 40 4 3 2 1 1 2 1924 2 41 1 3 2 1 2 1 2136 2 42 2 3 2 1 2 1 1878 2 43 3 3 2 1 2 1 2163 2 44 4 3 2 1 2 1 1822 2 45 1 3 2 1 2 2 2491 2 46 2 3 2 1 2 2 2389 2 47 3 3 2 1 2 2 1483 1 1 2 48 4 3 2 1 2 2 2133 2 17 1 2 2 2 1 1 1691 0.64 0.31 1.65 10.82 5.24 27.90 2 18 2 2 2 2 1 1 1699 0.69 0.29 1.73 11.72 4.93 29.39 2 19 3 2 2 2 1 1 1191 0.75 0.3 1.63 8.93 3.57 19.41 2 20 4 2 2 2 1 1 1617 0.58 0.27 1.6 9.38 4.37 25.87 2 21 1 2 2 2 1 2 1573 0.6 0.31 1.66 9.44 4.88 26.11 2 22 2 2 2 2 1 2 1876 0.62 0.31 1.79 11.63 5.82 33.58 2 23 3 2 2 2 1 2 1263 058 (1.27 1.56 7.33 341 19.70 2 24 4 2 2 2 1 2 1028 0,5Q 0.32 1.86 6.07 3.29 19.12 2 25 1 2 2 2 2 1 1637 0.66 (1.28 1.46 10.80 4.58 23.90 2 26 2 2 2 2 2 1 1598 0.66 0.27 1.58 10.55 4.31 25.25 2 27 3 2 2 2 2 1 1841 0.65 0.29 1.66 11.97 5.34 30.56 Caup 137 Appendix 3.3. Continued. seed pit YR # rep bc fert gr K si yld 28 4 2 2 2 29 1 2 2 30 2 2 2 31 3 TN Mg K TP 1812 0.66 0.31 1.7 1196 5.62 30.80 kg/ha 2 SEED SEED Ca Kup Pup Nup 2 2 1 2 2 2 2 1824 0.64 0.3 1.63 11.67 5.47 29.73 2 2 2 2 1684 0.65 0.27 1.54 10.95 4.55 25.93 2 2 2 2 2 1958 0.62 0.28 1.51 12.14 5.48 29.57 2 32 4 2 2 2 2 2 1836 0.67 0.33 1.82 12.30 6.06 33.42 2 49 1 4 1 2 1 1 1475 0.5 0.27 1.43 7.38 3.98 21.09 2 50 2 4 1 2 1 1 1501 0.51 0.29 1.59 7.66 4.35 23.87 2 51 3 4 1 2 1 1 1886 0.52 0.31 1.73 9.81 5.85 32.63 2 52 4 4 1 2 1 1 1523 0.51 0.32 1.79 7.77 4.87 27.26 2 53 1 4 1 2 1 2 1394 0.49 0.29 1.64 6.83 4.04 22.86 2 54 2 4 1 2 1 2 1377 0.46 0.3 1.68 6.33 4.13 23.13 2 55 3 4 1 2 1 2 1559 0.48 0.29 1.66 7.48 4.52 25.88 2 56 4 4 1 2 1 2 1535 0.51 0.35 1.88 7.83 5.37 28.86 2 57 1 4 1 2 2 I 1795 0.51 0.33 1.92 9.15 5.92 34.46 2 58 2 4 1 2 2 1 2(115 0.52 0.3 1.7 10.48 6.05 34.26 2 59 3 4 1 2 2 1 1550 0.58 0.31 1.66 8.99 4.81 25.73 2 60 4 4 1 2 2 1 1447 0.56 (1.31 1.7 8.11) 4.49 24.60 2 61 1 4 1 2 2 2 1501 0.5 0.28 1.55 7.51 4.2(1 23.27 2 62 2 4 1 2 2 2 1795 0.5 1)35 1.85 8.98 6.28 33.21 2 63 3 4 1 2 2 2 1663 (1.53 0.33 1.79 8.81 5.49 29.77 2 64 4 4 1 2 2 2 1415 (1.58 (1.33 1.71 8.21 4.67 24.20 Caup 3 1 1 1 1 1 1 1 1176 0.49 13.34 0.31) 0.21 5.80 3.95 3.51 3 2 2 1 1 1 1 1 896 0.50 0.33 0.31 (1.21 4.51 2.95 2.73 3 3 3 I 1 1 1 1 1034 0.52 11.37 0.26 (1.21 5.38 3.80 2.67 3 4 4 1 I 1 1 1229 0.47 0.37 0.25 0.21 5.84 4.50 3.12 3 5 1 1 1 1 1 2 1139 0.49 0.36 0.26 0.21 5.59 4.14 3.00 3 6 2 1 1 1 1 2 1117 0.48 0.36 (1.31 0.21 5.39 4.03 3.41 3 7 3 1 1 1 1 2 1116 (1.47 0.35 0.3(1 0.22 5.21 3.94 3.34 3 8 4 1 1 1 1 2 1010 (1.47 0.36 0.27 0.22 4.73 3.60 2.76 3 9 1 1 1 I 2 1 836 0.52 0.38 0.30 0.311 4.44 3.24 2.57 3 10 2 1 1 1 2 1 1001 (1.48 0.36 0.23 0.2(1 4.83 3.63 2.81 3 11 3 1 1 1 2 1 1198 (1.47 0.36 (1.26 (1.21 5.66 4.32 3.12 3 12 4 1 I 1 2 I 982 (1.46 11.37 (1.27 (1.21 4,49 3.59 2.67 3 13 1 1 1 1 2 2 1176 1)49 0.42 0.28 0.22 5.81 4.94 3.24 3 14 2 1 1 1 2 2 1146 (1,44 (1.39 0.27 0.21 5.09 4.49 3.08 3 15 3 1 1 1 2 2 1228 (1.48 0.38 0.27 0.22 5.87 4.68 3.29 3 16 4 1 1 1 2 2 1125 0.46 0.36 0.26 0.21 5.22 4.01 2.91 3 33 1 3 2 1 1 1 1715 0.60 1)42 0.22 0.21 10.31 7.29 3.83 1 138 Appendix 3.3. Continued. seed pit YR # rep bc fert gr st K yld SEED SEED TN Mg K TP 2 3 2 1 1 1 1521 0.57 0.39 0.21 0.21 8.64 5.97 3.22 35 3 3 2 1 1 1 1434 0.59 0.42 0.21 0.20 8.49 6.03 2.94 3 36 4 3 2 1 1 1 1709 0.58 0.41 0.23 0.19 9.97 7.07 3.98 3 37 1 3 2 1 1 2 1712 0.56 0.41 0.21 0.20 9.65 7.06 3.66 3 38 2 3 2 1 1 2 1315 0.64 0.46 0.23 0.24 8.39 6.02 3.01 3 39 3 3 2 1 1 2 1379 0.61 0.42 0.19 0.21 8.47 5.78 2.56 3 40 4 3 2 1 1 2 1648 0.61 0.42 0.24 0.20 9.99 6.91 3.98 3 41 1 3 2 1 2 1 1466 0.61 0.43 0.20 0.20 8.97 6.26 2.94 3 42 2 3 2 1 2 1 1347 0.54 0.40 0.21 0.20 7.27 5.44 2.82 5.97 2.73 kg/ha 3 34 3 Ca Kup Pup Nup Caup 3 43 3 3 2 1 2 1 1359 0.59 0.44 0.20 0.21 7.95 3 44 4 3 2 1 2 1 1108 0.66 0.42 0.21 0.20 7.29 4.61 2.30 3 45 1 3 2 1 2 2 1428 0.59 0.40 0.22 0.22 8.42 5.78 3.17 3 46 2 3 2 1 2 2 1565 0.66 0.35 0.23 0.23 10.32 5.50 3.61 3 47 3 3 2 1 2 2 1654 0,69 0.38 0.21 0.23 11.45 6.32 3.46 3 48 4 3 2 1 2 2 1340 0.68 0.39 0.19 0.22 9,15 5.23 2.53 3 17 1 2 2 2 1 1 1943 0.66 0.42 0.19 0.16 11.67 8.08 3.76 3 18 2 2 2 2 1 1 1713 0.56 0.37 0.19 0.15 9.60 6.37 3.17 3 19 3 2 2 2 1 1 2143 0.52 0.36 0.21 0.15 11.12 7.78 4.51 3 20 4 2 2 2 1 1 1623 0.56 0.40 0.17 0.16 9.17 6.46 2.81 0.58 0,39 0.21 0.16 11.26 7.47 3.98 3.40 3 21 1 2 2 2 1 2 1934 3 22 2 2 2 2 1 2 1799 0.55 0.39 0.19 0.16 9.82 6.98 3 23 3 2 2 2 1 2 1862 0.55 0.40 0.19 0.16 10.29 7.37 3.48 3 24 4 2 2 2 1 2 2258 (1.56 0.38 0.20 0.15 12.6$ 8.55 4.48 3 25 I 2 2 2 2 1 1654 1)55 0.37 0.19 (1.15 9.14 6.14 3.11 3 26 2 2 2 2 2 1 2052 ().6 I 0.40 0.19 0.15 12.49 8.26 3.97 3 27 3 2 2 2 2 1 1695 0.58 0.38 0.21) 0.16 9.76 6,41) 3.32 3 28 4 2 2 2 2 1 1670 1)59 0.38 0.19 0.15 9.93 6.40 3.18 3 29 1 2 2 2 2 2 1812 0.55 0.38 0.19 0.16 9.94 6.84 3.52 3 30 2 2 2 2 2 2 1523 0.62 (1.39 0.21 0.16 9.48 5.87 3.19 3 31 3 2 2 2 2 2 1478 0.58 0.39 0.19 0.16 8.64 5.70 2.83 3 32 4 2 2 2 2 2 1574 ((.60 0.39 0.19 0.16 9.44 6.16 3.01 3 49 1 4 1 2 1 1 2130 ((.53 0.38 0.22 0.17 11.18 8.01 4.77 3 51) 2 4 1 2 1 1 1255 (1.5$ (1.37 0.21 (1.17 7.23 4.64 2.66 3 51 3 4 1 2 1 1 1663 (1.53 (1.36 (1.23 (1.17 $77 5.92 3.83 0.56 3 52 4 4 1 1875 0.32 (1.24 0.16 10.43 6.06 4.58 3 53 1 4 1 2 1 2 2023 0.49 0.30 0.27 ((.16 9.94 6.14 5.55 3 54 2 4 1 2 1 2 1444 0.48 0.31 0.27 ((.17 6.93 4.51 3.92 1 2 1 139 Appendix 3.3. Continued. seed pit YR # rep toe len gr St K yld kg/ha SEED SEED K TP TN Ca Mg Kup Pup Nup Caup 2 1885 0.51 0.37 0.24 0.18 9.68 7.05 4.50 1 2 1649 0.54 0.39 0.23 0.18 8.89 6.45 3.77 2 2 1 1682 0.63 0.38 0.20 0.18 10.67 6.43 3.37 1 2 2 1 1762 0.62 0.33 0.23 0.16 10.97 5.82 4.13 1 2 2 1 1666 0.61 0.36 0.20 0.17 10.14 6.02 3.30 4 1 2 2 1 1682 0.59 0.40 0.19 0.17 9.94 6.69 3.18 4 1 2 2 2 1700 0.61 0.37 0.21 0.18 10.45 6.23 3.62 2 4 1 2 2 2 1820 0.58 0.39 0.22 0.18 10.64 7.13 4.00 63 3 4 1 2 2 2 1882 0.60 0.34 0.22 0.16 11.38 6.39 4.13 64 4 4 1 2 2 2 1795 0.64 0.36 0.21 0.16 11.50 6.41 3.69 3 55 3 3 4 56 4 4 1 2 3 57 1 4 1 3 58 2 4 3 59 3 4 3 60 4 3 61 1 3 62 3 3 1 2 1 140 Appendix 4 Data from chapter 5 Appendix 4.1 Soil solution and soil test levels at Hyslop in chapter 5. lime rate: 1=incorporatcd, 2=surface, 3=unlimed P rate, lb/a: 1=30,2= zero SOIL SOLUTION LEVELS lim P rep pH PO4 NO3 TP SOIL. TEST LEVELS S K CA Mg Zn Mn Cu Fe B AL Na pH WOl' K EOP Ca Mg mg/I mg/I mg/I mg/I mg/i mg/I mg/i mg/I mg/I mg/I mg/I mg/I mg/I mg/I 78.92 4.25 0.04 0.03 0.01 0.04 0.05 0.00 2.41 mg/kg mg/kg mg/k meq/lOOg6.2 141 242 92 10.5 0.75 1.69 6.3 98 - 1 1 1 6.55 0.39 0.42 0.46 68.35 8.43 1 1 2 5.34 0.14 1.25 0.47 41.96 18.21 55.44 3.36 0.04 0.17 0.02 0.04 0.09 0.27 1 1 3 6.79 0.28 0.00 0.51 85.37 15.77 110.51 6.73 0.03 0.06 0.04 0.04 0.07 0.42 2.74 6.5 1 1 4 6.81 0.21 0.23 0.12 48.98 10.81 62.20 3.54 0.02 0.05 0.00 0.02 0.05 0.00 2.55 6.6 1 2 1 6.41 0.07 0.29 0.50 93.07 19.24 106.43 6.02 0.02 0.09 0.01 0.02 0.05 0.05 2.02 1 2 2 6.52 0.07 0.00 0.49 57.82 13.81 78.27 4.25 0.03 0.03 0.02 0.03 0.12 0.33 2.47 1 2 3 7.07 0.09 0.00 0.39 50.24 11.09 68.92 3.54 0.00 0.00 0.00 0.01 0.00 0.00 2.05 7.1 1 2 4 6.74 0.10 0.00 0.16 63.65 10.51 79.30 4.25 0.01 0.01 0.00 0.01 0.05 0.04 2.46 2 1 1 5.44 0.07 0.00 0.28 77.22 11.82 83.31 4.96 0.05 0.54 0.01 0.04 0.07 0.21 2.20 2 I 2 4.54 0.09 0.00 0.10 120.96 15.92 138.69 7.79 0.08 0.76 0.03 0.04 0.07 0.88 2 1 3 6.70 0.17 0.00 0.07 49.31 15.71 72.07 4.25 0,03 0.06 0.05 0.04 0.07 0.49 2 1 4 5.47 0.12 0.00 0.79 70.30 10.61 72.72 4.60 0.06 0.08 0.00 0.01 0.05 0.00 2 2 1 5.98 0.03 0.00 0.08 88.28 13.37 95.83 4.25 0.08 0.47 0.00 0.04 0.05 2 2 2 4.10 0.07 0.00 0.27 119.97 23.40 131.53 9.03 0.07 0.46 0.05 0.07 2 2 3 5.78 0,05 0.00 (1.36 54.44 16.19 67.51 4.25 0.05 0.13 0.04 2 2 4 6.35 0.07 0.31 0.22 85.53 15.12 100.36 6.02 0.05 0.07 0.04 3 1 1 3.96 0.24 0.00 0.14 47.61 16.71 41.41 4.43 0.12 0.88 3 1 2 3.73 0.11 0.00 0.27 53.69 18.80 52.35 5.31 0.07 3 1 3 4.24 0.31 0.00 0.46 26.40 19.23 19.72 2.13 0.06 3 1 4 3.87 0.20 1.68 0.26 61.23 12.57 59.40 5,67 3 2 1 4.33 0.06 0.27 0.50 33.27 28.23 27.53 3 2 2 3.83 0.07 0.00 0.68 49.34 24.15 3 2 3 4.08 0.06 0.00 0.18 32.99 3 2 4 4.38 0.10 0.00 0.50 63.41 218 80 11.2 0.72 117 254 76 11.6 0.82 94 289 71 12.2 0.81 6.4 81 273 56 11.2 0.75 5.2 74 261 53 11.1 0.72 76 222 62 14.0 0.73 6.5 66 285 53 12.4 0.81 5.0 118 242 81 6.0 0.43 1.79 5.1 126 226 85 7.6 0.52 2.24 5.6 108 316 68 8.8 0.65 2.03 5.8 106 187 51 13.0 0.57 0.09 2.40 5.1 104 226 69 7.1 0.36 0.07 0.75 3.08 5.3 99 250 65 7.2 0.69 0.05 0.07 0.54 2.74 5.3 84 261 52 6.8 0.52 0.04 0.07 0.38 2.54 5.8 74 234 52 10.3 0.65 0.00 0.07 0.19 1.25 2.86 4.6 138 265 97 2.8 0.4 0.87 0.02 0.05 0.07 1.23 2.30 4.5 141 234 108 3.0 0.56 0.18 0.01 0.01 0.05 0.11 2.77 4.9 115 296 81 4.5 0.6 0.10 0.91 0.02 0.05 0.05 0.58 3.30 4.8 116 257 83 4.8 0.63 2.48 0.06 0.20 0.02 0.04 0.07 0.38 1.98 4.9 89 371 58 3.6 0.55 43.34 4.25 0.11 0.68 0.03 0.06 0.07 1.21 2.52 4.6 92 339 66 2.7 0.42 9.63 19.89 3.19 0.06 0.18 0.00 0.01 0.00 0.00 1.71 4.8 75 250 57 4.1 0.57 13.89 72.03 5.84 0.09 0.17 0.05 0.08 0.09 0.64 2.81 5.2 89 257 58 6.4 0.73 H H Appendix 4.2 Soil solution and soil test values from Saddle Butte in chapter 5. lime: 1=incorporated, 2=surface, 3=unlimed P: 1=30, 2= zero SOIL TEST LEVELS SOIL SOLUTION LEVELS lini P rep pH PO4 NO3 TP S K CA Mg Zn Mn Cu Fe B mg/i mg/i mg/i mg/i mg/i mg/I mg/i mg/i mg/I mg/i AL Na p11 WOP K EOP Ca Mg mg/I mg/i mg/i mg/I mg/i I 2 3 6.68 0.07 46.06 0.64 154.44 25.62 198.92 24.26 0.02 0.13 0.06 0.07 0.07 0.56 10.84 6.4 9 234 17 26.3 3.4 1 2 2 6.12 0.06 70.95 0.09 169.94 19.15 211.73 28.69 0.01 0.48 0.00 0.01 0.00 0.00 11.68 6.2 20 218 33 25.8 3.4 1 2 1 6.22 0.10 2.43 0.43 106.14 14.26 117.84 16.82 0.07 037 0.08 0.07 0.09 0.74 7.41 6.9 16 230 30 32.7 3.2 1 1 3 6.93 0.09 39.46 0.31 114.48 15.77 169.91 21.61 0.02 0.07 0.06 0.05 0.07 0,70 6.17 6.4 15 168 26 28.6 3.4 I I 2 5.14 0.12 9.34 0.44 171.44 15.15 160.46 20.37 0.09 0.15 0.02 0.01 0.00 0.00 6.47 5.8 30 195 43 22.5 3.3 I I 1 7.00 0.12 2.08 0.43 161.31 19.95 182.56 22.49 0.03 0.03 0.07 0.04 0.05 0.56 10.55 7.0 19 176 39 32.5 3.7 2 2 3 4.20 0.05 20.55 0.31 148.98 15.92 150.08 26.39 0.13 3.97 0.05 0.07 0.07 0.80 10.13 5.1 12 172 22 19.8 4.1 2 2 2 4.29 0.05 29.82 0.44 171.50 12.52 167.77 30.46 0.08 3.86 0.00 0.03 0.00 0.06 8.16 5.0 14 160 25 19.6 3.9 2 2 1 4.40 0.06 45.91 0.13 151.92 19.80 169.52 29.75 0.18 400 0.05 0.08 0.07 0.52 14.66 5.4 12 199 21 22.3 4.4 2 1 3 6.08 0.12 3.73 0.76 12733 14.48 130.77 21.25 0.10 1.28 0.08 0.11 0.07 0.84 7.77 5.2 14 133 31 21.5 4.2 2 1 2 5.88 0.05 22.95 0.47 181.82 14.67 180.67 27.27 0.08 0.30 0.02 0.04 0.05 0.19 7.80 5.6 23 164 31 22.5 3.8 2 1 1 5.03 0.12 6.82 0.85 142.35 14.51 151.17 22.31 0.11 0.92 0.06 0.05 0.09 0.55 8.95 5.5 36 160 54 21.0 3.8 3 2 3 3.68 0.04 22.45 0.42 120.48 13.12 118.77 24.09 (1.10 3.88 0.03 0.07 0.05 0.65 9.55 4.3 10 183 22 15.1 3.9 3 2 2 3.89 0.04 41.65 0.46 119.13 13.39 124.97 25.86 0.11 4.13 0.00 0.05 0.05 0.28 10.45 4.8 14 164 22 18.3 4.1 3 2 1 5.30 0.17 7.79 0.73 113.86 16.95 114.91 19.13 0.13 2.09 0.09 0.07 0.09 0.51 12.54 5.1 21 187 31 18.6 3.8 3 1 3 4.34 0.10 22.75 0.37 131.13 15.22 136.34 24.26 0.12 2.78 0.06 0.10 0.07 0.91 10.77 4.9 13 137 27 17.9 4.1 3 1 2 4.10 0.12 16.33 (1.46 123.01 12.49 107.59 24.44 0.11 5.56 0.02 0.08 0.05 0.52 11.03 4.7 24 211 41 16.4 4.2 3 1 1 3.95 0.09 0.97 0.38 96.07 11.68 97.63 17.53 0.13 4.46 0.09 0.11 0.09 0.98 7.72 4.7 26 218 39 15.5 3.9 mg/kg mg/kg mg/kg --meq/IOOg-- H NJ