Product datasheet Anti-RPS2 antibody [EPR10834(B)] ab155961 4 Images Overview Product name Anti-RPS2 antibody [EPR10834(B)] Description Rabbit monoclonal [EPR10834(B)] to RPS2 Tested applications WB, IHC-P, ICC/IF Species reactivity Reacts with: Mouse, Rat, Human Immunogen Synthetic peptide corresponding to residues in Human RPS2 (P15880) Positive control HeLa, A549 or 293T cell lysate, Human kidney or pancreas tissue, HeLa cells. General notes This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. Properties Form Liquid Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. Storage buffer Preservative: 0.01% Sodium azide Constituents: 50% Glycerol, 0.05% BSA Purity Tissue culture supernatant Clonality Monoclonal Clone number EPR10834(B) Isotype IgG Applications Our Abpromise guarantee covers the use of ab155961 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application WB Abreviews Notes 1/1000 - 1/10000. Predicted molecular weight: 31 kDa. 1 Application Abreviews IHC-P Notes 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended. ICC/IF Application notes 1/100 - 1/250. Is unsuitable for Flow Cyt or IP. Target Sequence similarities Belongs to the ribosomal protein S5P family. Contains 1 S5 DRBM domain. Anti-RPS2 antibody [EPR10834(B)] images All lanes : Anti-RPS2 antibody [EPR10834(B)] (ab155961) at 1/1000 dilution Lane 1 : HeLa cell lysate Lane 2 : A549 cell lysate Lane 3 : 293T cell lysate Lysates/proteins at 10 µg per lane. Secondary Western blot - Anti-RPS2 antibody HRP labelled goat anti-rabbit at 1/2000 [EPR10834(B)] (ab155961) dilution Predicted band size : 31 kDa Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling RPS2 with ab155961 at a 1/1000 dilution. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961) 2 Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling RPS2 with ab155961 at a 1/50 dilution. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961) Immunofluorescence analysis of HeLa cells labeling RPS2 with ab155961 at a 1/100 dilution. Immunocytochemistry/ Immunofluorescence Anti-RPS2 antibody [EPR10834(B)] (ab155961) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3