Product datasheet
Anti-CD49b antibody [TEA1/41] (CF405M) ab115797
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Overview
Product name
Anti-CD49b antibody [TEA1/41] (CF405M)
Description
Mouse monoclonal [TEA1/41] to CD49b (CF405M)
Conjugation
CF405M. Ex: 408nm, Em: 452nm
Tested applications
Flow Cyt
Species reactivity
Reacts with: Human
Immunogen
The details of the immunogen for this antibody are not available.
Positive control
Human platelets (peripheral blood).
General notes
CF405M (Abs/Em Max: 408/450 nm) US patent application no. 12/607,915. CF405M (Abs/Em
Max: 408/450nm). Direct replacement for Pacific Blue dye®, BD Horizon™ V450.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C.
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Purity
Protein G purified
Clonality
Monoclonal
Clone number
TEA1/41
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab115797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Flow Cyt
Abreviews
Notes
Use 5µl for 106 cells.
ab126026 - Mouse monoclonal IgG1, is suitable for use as an isotype control with
this antibody.
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Target
Relevance
Human CD49b is an integrin alpha 2 subunit that forms a heterodimer with beta 1 integrin and
functions as an adhesion molecule. CD49b is a 160 kDa glycoprotein that noncovalently
associates with the 130 kDa integrin beta 1 subunit (CD29) to form glycoprotein Ia/IIa (when
expressed on platelets), and very late (activation) antigen 2 ('VLA2') when found on T cells.
CD49b is expressed by platelets, approximately 50% of monocytes, long term cultivated T cells
and most adherent cell lines. Integrin alpha 2/beta 1 is a receptor for laminin, collagen, collagen
C propeptides, fibronectin and E cadherin. It recognizes the proline hydroxylated sequence G-FP-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens,
modulation of collagen and collagenase gene expression, force generation and organization of
newly synthesized extracellular matrix.
Cellular localization
Cell Membrane; Single pass type I membrane protein.
Anti-CD49b antibody [TEA1/41] (CF405M) images
ab115797 at 5µl/106 cells staining CD49b in
Human platelets by Flow Cytometry (shown in
red).
Isotype control (blue).
Flow Cytometry - Anti-CD49b antibody [TEA1/41]
(CF405M) (ab115797)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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