Product datasheet
Anti-MASP2 antibody - Carboxyterminal end ab65898
Overview
Product name
Anti-MASP2 antibody - Carboxyterminal end
Description
Rabbit polyclonal to MASP2 - Carboxyterminal end
Specificity
ab65898 recognizes full length MASP2, but not the Map19 form, MASP1 or MASP3.
Tested applications
WB
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide based on the carboxyterminal end of human MASP2.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab65898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for
chemiluminescent substrates. Predicted molecular weight: 76 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
1
Relevance
MASP2, MBL associated serine protease 2, is a serum protease that plays an important role in
the activation of the complement system via mannose-binding lectin (MBL). Binding of MBL to
carbohydrate groups triggers MASP2 activation by auto-catalytic cleavage which leads to
cleavage of C2 and C4, leading to their activation and to the formation of C3 convertase.
MASP2 also binds to L-Ficolin and H-Ficolin, and when the complex binds pathogens the MASP
is activated. Low MBL levels in serum are associated with increased susceptibility to infection,
and an Asp105Gly mutation in MASP2 is associated with low MASP2 levels in serum. Reduced
functional levels of MBL are also postulated to be associated with generation of autoantibodies
in systemic lupus erythematosus, acting through a decreased clearance of apoptotic material. A
truncated form of MASP2 that constitutes the amino 185 residues, Map19 is also a component
of the MBL-MASP-1 complex.
Cellular localization
Secreted
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
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