Product datasheet
Anti-MASP2 antibody ab65895
1 Image
Overview
Product name
Anti-MASP2 antibody
Description
Rabbit polyclonal to MASP2
Specificity
ab65895 recognizes full-length MASP2 and the Map19 form, but not MASP1 or MASP3. We
have a range of domain specific antibodies for this target. For a full list please see all MASP2
antibodies.
Tested applications
WB
Species reactivity
Reacts with: Human
Immunogen
A synthetic peptide based on the end of the first CUB domain, beginning of the EGF-like domain
of human MASP2.
Positive control
SK HEP 1 lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab65895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for
chemiluminescent substrates. Detects a band of approximately 76 kDa (predicted molecular
weight: 76 kDa).
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Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
MASP2, MBL associated serine protease 2, is a serum protease that plays an important role in
the activation of the complement system via mannose-binding lectin (MBL). Binding of MBL to
carbohydrate groups triggers MASP2 activation by auto-catalytic cleavage which leads to
cleavage of C2 and C4, leading to their activation and to the formation of C3 convertase.
MASP2 also binds to L-Ficolin and H-Ficolin, and when the complex binds pathogens the MASP
is activated. Low MBL levels in serum are associated with increased susceptibility to infection,
and an Asp105Gly mutation in MASP2 is associated with low MASP2 levels in serum. Reduced
functional levels of MBL are also postulated to be associated with generation of autoantibodies
in systemic lupus erythematosus, acting through a decreased clearance of apoptotic material. A
truncated form of MASP2 that constitutes the amino 185 residues, Map19 is also a component
of the MBL-MASP-1 complex.
Cellular localization
Secreted
Anti-MASP2 antibody images
All lanes : Anti-MASP2 antibody (ab65895)
at 1/5000 dilution
Lane 1 : SK HEP 1 lysate at 30 µl
Lane 2 : SK HEP 1 lysate at 20 µl
Lane 3 : SK HEP 1 lysate at 15 µl
Western blot - MASP2 antibody (ab65895)
Predicted band size : 76 kDa
Observed band size : 76 kDa
Additional bands at : 100 kDa,36 kDa,45
kDa,50 kDa. We are unsure as to the identity
of these extra bands.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
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Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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