Product datasheet Anti-Human Kappa Chain antibody [EPR11633] ab170879 1 Image Overview Product name Anti-Human Kappa Chain antibody [EPR11633] Description Rabbit monoclonal [EPR11633] to Human Kappa Chain Tested applications WB Species reactivity Reacts with: Human Does not react with: Mouse, Rat Immunogen Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Human Kappa Chain. The exact sequence is proprietary. Database link: P01834 Positive control Human spleen, Human tonsil and Human fetal liver lysates General notes This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. Properties Form Liquid Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Storage buffer Preservative: 0.01% Sodium azide Constituents: 50% Glycerol, 0.05% BSA Purity Tissue culture supernatant Clonality Monoclonal Clone number EPR11633 Isotype IgG Applications Our Abpromise guarantee covers the use of ab170879 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. 1 Application Abreviews WB Application notes Notes 1/10000 - 1/50000. Predicted molecular weight: 12 kDa. Is unsuitable for ICC,IHC-P or IP. Target Relevance Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. Cellular localization Cytoplasmic Anti-Human Kappa Chain antibody [EPR11633] images All lanes : Anti-Human Kappa Chain antibody [EPR11633] (ab170879) at 1/10000 dilution Lane 1 : Human spleen lysate Lane 2 : Human tonsil lysate Lane 3 : Human fetal liver lysate Lysates/proteins at 10 µg per lane. Secondary Western blot - Anti-Human Kappa Chain HRP labeled goat anti-rabbit IgG at 1/2000 [EPR11633] antibody (ab170879) dilution Predicted band size : 12 kDa Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. 2 Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3