Product datasheet
Anti-SH3GL2 antibody [EPR10215(B)] ab150431
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Overview
Product name
Anti-SH3GL2 antibody [EPR10215(B)]
Description
Rabbit monoclonal [EPR10215(B)] to SH3GL2
Tested applications
WB
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
Positive control
Human cerebellum, Mouse brain and Rat brain lysates.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20ºC.
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR10215(B)
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab150431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Application notes
Abreviews
Notes
1/10000 - 1/50000. Predicted molecular weight: 40 kDa.
Is unsuitable for ICC,IHC-P or IP.
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Target
Function
Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high
curvature.
Tissue specificity
Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.
Sequence similarities
Belongs to the endophilin family.
Contains 1 BAR domain.
Contains 1 SH3 domain.
Domain
An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into
helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved
membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the
pair of second amphipathic helices protruding towards the membrane-binding surface.
Cellular localization
Cytoplasm. Membrane. Concentrated in presynaptic nerve terminals in neurons.
Anti-SH3GL2 antibody [EPR10215(B)] images
All lanes : Anti-SH3GL2 antibody
[EPR10215(B)] (ab150431) at 1/10000
dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Western blot - Anti-SH3GL2 antibody
[EPR10215(B)] (ab150431)
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 40 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Valid for 12 months from date of delivery
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Terms and conditions
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