Product datasheet
Anti-LAIR1 antibody [113] ab95748
1 References 1 Image
Overview
Product name
Anti-LAIR1 antibody [113]
Description
Armenian Hamster monoclonal [113] to LAIR1
Tested applications
Flow Cyt
Species reactivity
Reacts with: Mouse
Immunogen
ARHO12 clone stably expressing mouse LAIR1 fused to a C terminal MYC tag.
Positive control
C57BL/6 splenocytes
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze /
thaw cycle.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2
Purity
Protein G purified
Clonality
Monoclonal
Clone number
113
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab95748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
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Application
Abreviews
Notes
Use 0.125-0.25µg for 105-8 cells. A 100 µl volume is recommended for staining
Flow Cyt
the cell sample.
ab18479-Armenian Hamster monoclonal IgG, is suitable for use as an isotype
control with this antibody.
Target
Function
Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic
function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in
recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the
increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory
role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ Tcells, downregulating IL2 and IFNG production while inducing secretion of transforming growth
factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF
secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as
prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of Ikappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors
towards dendritic cells.
Tissue specificity
Expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, Tcells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no
expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1
infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr
virus infection.
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Developmental stage
Complete loss of expression when naive B-cells proliferates and differentiates into Ig-producing
plasma cells under in vitro stimulation.
Domain
ITIM (immunoreceptor tyrosine-based inhibitor motif) motif is a cytoplasmic motif present in 2
copies in the intracellular part of LAIR1. When phosphorylated, ITIM motif can bind the SH2
domain of several SH2-containing phosphatases, leading to down-regulation of cell activation.
Post-translational
modifications
Phosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK.
N-glycosylated.
Cellular localization
Cell membrane.
Anti-LAIR1 antibody [113] images
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Staining of C57BL/6 splenocytes with 0.125
µg of Armenian Hamster IgG Isotype Control
Purified (purple line) or 0.125 µg of ab95748
(blue line) followed by Anti-Armenian Hamster
IgG PE. Total viable F4/80+ cells were used
for analysis.
Flow Cytometry - LAIR1 antibody [113] (ab95748)
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