Product datasheet
Anti-SWAP70 antibody ab150325
2 Images
Overview
Product name
Anti-SWAP70 antibody
Description
Rabbit polyclonal to SWAP70
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human
SWAP70 (NP_055870.2).
Run BLAST with
Positive control
Run BLAST with
Human tonsil (germinal centre) tissue; Human spleen cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab150325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 69 kDa.
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with
citrate buffer pH 6 before commencing with IHC staining protocol.
Target
1
Function
Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF)
which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also
mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or
adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate
production and cell protrusion.
Tissue specificity
Expressed only in mature B-cells including those associated with mucosa-associated tissue and
bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and
fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages
(PubMed:12925760).
Sequence similarities
Contains 1 PH domain.
Domain
The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
Post-translational
modifications
Tyrosine-phosphorylated.
Cellular localization
Cytoplasm. Cell membrane. Nucleus. Cell projection > lamellipodium. In resting B-cells it is
localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane
and then translocates to the nucleus. In activated, class-switching B-cells it is associated with
membrane IgG but not IgM. Localized to loose actin filament arrays located behind actively
extending lamellipodia.
Anti-SWAP70 antibody images
Immunohistochemical analysis of Formalinfixed, paraffin-embedded Human tonsil
(germinal centre) tissue labeling SWAP70
with ab150325 at 10µg/ml.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-SWAP70
antibody (ab150325)
All lanes : Anti-SWAP70 antibody
(ab150325) at 0.25 µg/ml
Lane 1 : Human spleen cell lysate
Lane 2 : Human spleen cell lysate with
immunising peptide
Predicted band size : 69 kDa
Western blot - Anti-SWAP70 antibody (ab150325)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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