Product datasheet
Anti-CD11c antibody [3.9] (Allophycocyanin) ab95764
1 Abreviews 2 Images
Overview
Product name
Anti-CD11c antibody [3.9] (Allophycocyanin)
Description
Mouse monoclonal [3.9] to CD11c (Allophycocyanin)
Conjugation
Allophycocyanin. Ex: 645nm, Em: 660nm
Tested applications
Flow Cyt
Species reactivity
Reacts with: Human
Immunogen
Human rheumatoid synovial fluid cells/monocytes.
Positive control
Normal Human peripheral blood cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 1X PBS, pH 7.2
Purity
Protein G purified
Clonality
Monoclonal
Clone number
3.9
Isotype
IgG1
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab95764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Flow Cyt
Abreviews
Notes
Use 5µl for 106 cells. ab37391-Mouse monoclonal IgG1, is suitable for use as an
isotype control with this antibody.
Target
1
Function
Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in
fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially
important in monocyte adhesion and chemotaxis.
Tissue specificity
Predominantly expressed in monocytes and granulocytes.
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo
protease cleavage.
Cellular localization
Membrane.
Anti-CD11c antibody [3.9] (Allophycocyanin) images
Staining of normal Human peripheral blood
cells with Mouse IgG1 ? Isotype Control APC
(open histogram) or ab95764 (filled
histogram). Cells in the monocyte gate were
used for analysis.
Flow Cytometry - CD11c antibody [3.9]
(Allophycocyanin) (ab95764)
ab36956 staining CD11c in the Human
PBMCs by Flow Cytometry. Cells were
harvested in a Ficoll-hypaque gradient. The
sample was incubated with the primary
antibody (1/100 in 2% Human sserum, 1mM
EDTA in PBS) for 25 minutes at 4°C.
Gating Strategy: Lymphocytes
Flow Cytometry - Anti-CD11c antibody [3.9]
(Allophycocyanin) (ab95764)
This image is courtesy of an anonymous Abreview
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