Product datasheet
Anti-LIME antibody ab17025
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Overview
Product name
Anti-LIME antibody
Description
Goat polyclonal to LIME
Tested applications
WB
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat
Immunogen
Synthetic peptide:
ENVYESIKEMGL
, corresponding to amino acids 258-269 of Mouse LIME.
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Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab17025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.
Target
Relevance
LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of
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T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the
CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its
negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of
Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3
stimulation.
Cellular localization
Cell membrane; Single-pass type III membrane protein. Present in lipid rafts. Recruited to the
immunological synapse upon conjugation of T cell with antigen presenting cell
Anti-LIME antibody images
ab17025 at 1ug/ml on mouse heart lysate
(35ug).
Western blot - LIME antibody (ab17025)
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Terms and conditions
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